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  4. Recombinant Mouse Prosaposin (Psap)

Recombinant Mouse Prosaposin (Psap)

  • 货号：
    CSB-YP736809MO
  • 规格：
  • 来源：
    Yeast
  • 其他：
  • 货号：
    CSB-EP736809MO
  • 规格：
  • 来源：
    E.coli
  • 其他：
  • 货号：
    CSB-EP736809MO-B
  • 规格：
  • 来源：
    E.coli
  • 共轭：
    Avi-tag Biotinylated

    E. coli biotin ligase (BirA) is highly specific in covalently attaching biotin to the 15 amino acid AviTag peptide. This recombinant protein was biotinylated in vivo by AviTag-BirA technology, which method is BriA catalyzes amide linkage between the biotin and the specific lysine of the AviTag.

  • 其他：
  • 货号：
    CSB-BP736809MO
  • 规格：
  • 来源：
    Baculovirus
  • 其他：
  • 货号：
    CSB-MP736809MO
  • 规格：
  • 来源：
    Mammalian cell
  • 其他：

产品详情

  • 纯度：
    >85% (SDS-PAGE)
  • 基因名：
    Psap
  • Uniprot No.：
  • 别名：
    Psap; Sgp1; Prosaposin; Sulfated glycoprotein 1; SGP-1) [Cleaved into: Saposin-A; Saposin-B-Val; Saposin-B; Saposin-C; Saposin-D]
  • 种属：
    Mus musculus (Mouse)
  • 蛋白长度：
    Full Length of Mature Protein
  • 表达区域：
    17-557
  • 氨基酸序列
    SPVQ DPKTCSGGSA VLCRDVKTAV DCGAVKHCQQ MVWSKPTAKS LPCDICKTVV TEAGNLLKDN ATQEEILHYL EKTCEWIHDS SLSASCKEVV DSYLPVILDM IKGEMSNPGE VCSALNLCQS LQEYLAEQNQ KQLESNKIPE VDMARVVAPF MSNIPLLLYP QDHPRSQPQP KANEDVCQDC MKLVSDVQTA VKTNSSFIQG FVDHVKEDCD RLGPGVSDIC KNYVDQYSEV CVQMLMHMQD QQPKEICVLA GFCNEVKRVP MKTLVPATET IKNILPALEM MDPYEQNLVQ AHNVILCQTC QFVMNKFSEL IVNNATEELL VKGLSNACAL LPDPARTKCQ EVVGTFGPSL LDIFIHEVNP SSLCGVIGLC AARPELVEAL EQPAPAIVSA LLKEPTPPKQ PAQPKQSALP AHVPPQKNGG FCEVCKKLVL YLEHNLEKNS TKEEILAALE KGCSFLPDPY QKQCDDFVAE YEPLLLEILV EVMDPGFVCS KIGVCPSAYK LLLGTEKCVW GPSYWCQNME TAARCNAVDH CKRHVWN
  • 蛋白标签：
    Tag type will be determined during the manufacturing process.
    The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.
  • 产品提供形式：
    Lyophilized powder
    Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
  • 复溶：
    We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
  • 储存条件：
    Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
  • 保质期：
    The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
    Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
  • 货期：
    Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
    Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
  • 注意事项：
    Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
  • Datasheet ：
    Please contact us to get it.

产品评价

靶点详情

  • 功能：
    Behaves as a myelinotrophic and neurotrophic factor, these effects are mediated by its G-protein-coupled receptors, GPR37 and GPR37L1, undergoing ligand-mediated internalization followed by ERK phosphorylation signaling.; Saposin-A and saposin-C stimulate the hydrolysis of glucosylceramide by beta-glucosylceramidase (EC 3.2.1.45) and galactosylceramide by beta-galactosylceramidase (EC 3.2.1.46). Saposin-C apparently acts by combining with the enzyme and acidic lipid to form an activated complex, rather than by solubilizing the substrate.; Saposin-B stimulates the hydrolysis of galacto-cerebroside sulfate by arylsulfatase A (EC 3.1.6.8), GM1 gangliosides by beta-galactosidase (EC 3.2.1.23) and globotriaosylceramide by alpha-galactosidase A (EC 3.2.1.22). Saposin-B forms a solubilizing complex with the substrates of the sphingolipid hydrolases.; Saposin-D is a specific sphingomyelin phosphodiesterase activator (EC 3.1.4.12).; Saposins are specific low-molecular mass non-enzymatic proteins, they participate in the lysosomal degradation of sphingolipids, which takes place by the sequential action of specific hydrolases.
  • 基因功能参考文献：
    1. Accumulating evidence suggests that PGRN is essential for proper lysosomal function, but the precise mechanisms involved are not known. Here, we show that PGRN facilitates neuronal uptake and lysosomal delivery of prosaposin (PSAP), the precursor of saposin peptides that are essential for lysosomal glycosphingolipid degradation. PMID: 28541286
    2. Both PSAP reduction and overexpression lead to significantly elevated extracellular progranulin (PGRN) levels. Intriguingly, PSAP knockdown increases PGRN monomers, whereas PSAP overexpression increases PGRN oligomers, partly through a protein-protein interaction. PMID: 27356620
    3. Data show that matrix (ECM) stiffness leads to mechano-signal transduction in mesenchymal stem cells (MSC), which promotes mammary tumor growth in part through secretion of the signaling protein prosaposin. PMID: 28972074
    4. Data suggested that the abundance of Psap in sperm sample may be a sensitive endpoint to predict PCB exposure. PMID: 26045750
    5. Prosaposin facilitates sortilin-independent lysosomal trafficking of progranulin. PMID: 26370502
    6. This study demonistrated that Spiral ganglion degeneration and hearing loss as a consequence of satellite cell death in saposin B-deficient mice. PMID: 25698761
    7. Mesotrypsin generated saposins A-D from prosaposin, and mature caspase-14 contributed to this process by activating mesotrypsinogen to mesotrypsin. Knockdown of these proteases markedly down-regulated saposin A synthesis in skin equivalent models. PMID: 24872419
    8. Low levels of prosaposin and its receptors in the mouse brain suggest the participation of prosaponin in pathological changes in the brains of dystrophic mdx mice. PMID: 24244600
    9. These data show functional association between GPR37, prosaposin, and GM1 ganglioside in the plasma membrane. PMID: 24371137
    10. The saposin A and saposin B deficiency AB(-/-) mice develop accumulation of multiple glycosphingolipids in various organs. PMID: 23697974
    11. Prosaposin is involved in the regulation of muscle differentiation of regenerated fibres PMID: 23325523
    12. Saposins A and B deficiencies attenuated GalCer-beta-galactosylceramidase and GM1-beta-galactosidase functions in the degradation of lactosylceramide in the liver. PMID: 23446636
    13. These data suggest that prosaposin plays an important role in the neuronal maturation processes of the vestibular sensory epithelium and the maintenance of normal vestibular system function. PMID: 22326583
    14. The saposin C deficient mice backcrossed to point mutated GCase mimics the central nervous system phenotype and biochemistry of some type 3 (neuronopathic) variants of Gaucher disease. PMID: 20047948
    15. These results suggest the in vivo role of saposin C in axonal membrane homeostasis, the disruption of which leads to neurodegeneration in lysosomal storage disease. PMID: 20175216
    16. UGT1 aids in the folding of sequential domain-containing proteins such as prosaposin. PMID: 20498017
    17. Saposin C has multiple roles in glycosphingolipid catabolism and functions in Central Nervous System independent of its role as an stabilizer of GCase. PMID: 20015957
    18. Immunoblot studies indicated that prosaposin, already abundant in the brain of WT, was dramatically increased in Sap-D(-/-) PMID: 19907127
    19. A saposin-like motif within the first half of the prosaposin C terminus contains the sortilin recognition site. PMID: 19934382
    20. The growth promotion and anti-apoptotic activity of prosaposin is partly through the PI3K/Akt signal pathway. PMID: 20042390
    21. reconstruction of the order of internal duplications that gave rise to the four saposins by using phylogenetic tools PMID: 11734895
    22. The lysosomal transport of prosaposin requires the conditional interaction of its highly conserved d domain with sphingomyelin PMID: 11856752
    23. prosaposin-/- mice showed seminolipid level at approximately 150% of the normal level at the terminal stage PMID: 12810822
    24. data indicate that saposin C is required for acid beta-glucosidase resistance to proteolytic degradation in the cell PMID: 12813057
    25. mice deficient in prosaposin, the precursor to a family of endosomal lipid transfer proteins (LTP), exhibit specific defects in CD1d-mediated antigen presentation and lack Valpha14 NKT cells PMID: 14684827
    26. The trafficking of prosaposin and GM2 activator protein to the lysosome in sertoli cells is dependent on sortilin. PMID: 15236333
    27. Mice which had a mutation introduced into the prosaposin gene in the saposin D domain developed progressive polyuria and ataxia and loss of cerebellar Purkinje cells. PMID: 15345707
    28. Data show that there is evolutionary conservation of the prosaposin structure and alternative splicing, and in expression in the mouse and chick developing brain. PMID: 15469878
    29. The prosaposin variant containing the exon 8-encoded three amino acids is dispensable for normal mouse development and fertility as well as for prosaposin secretion and its lysosomal function. PMID: 15743835
    30. Experiments with knock-out mice lacking prosaposin suggest that prosaposin helps maintain normal innervation patterns to the organ of Corti. PMID: 17167097
    31. Combined saposin C and D deficiencies in mice lead to a neuronopathic phenotype. PMID: 17353235
    32. These findings delineate the roles of saposin B for the in vivo degradation of several glycosphingolipid hydrolases and its primary function in maintenance of CNS function. PMID: 18480170
    33. inactivation of the sortilin gene produces a significant decrease of prosaposin in the lysosomes PMID: 19732768

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  • 亚细胞定位：
    [Prosaposin]: Secreted.; Lysosome.
  • 数据库链接：

    KEGG: mmu:19156

    STRING: 10090.ENSMUSP00000137476

    UniGene: Mm.277498