Recombinant Mouse Oncomodulin (Ocm)

In the production of recombinant Mouse Ocm protein, the gene for Ocm (Yeast) was cloned into a vector and expressed as Ocm protein in Yeast. The plasmids with the copy of Ocm, or the expression vector, were often used to enhance gene expression. Every step of production was undergone with a strict QC system. N-terminal 6xHis tag was used in the process. The purity is 90% determined by SDS-PAGE.

Oncomodulin (OCM) is a small EF-hand Ca2+-binding protein (CaBP) of approximately 12 kDa belonging to the parvalbumin family. Initially, OCM was considered oncogenic due to lack of evidence of any expression in normal post-embryonic tissue. However, decades after its initial discovery, OCM was identified as a major protein in sensory cells of the guinea pig cochlea. There are only a limited number of studies on the function of OCM largely due to its very restricted temporal and spatial expression patterns. Also, only a few OCM studies have addressed: intracellular concentration, affinity for metal ions, mobility, and Ca2+-sensing capacity. Existing findings show that OCM is evolutionarily distinguished from the majority of lower vertebrate β-parvalbumins. OCM may have an ambiguous function that depends upon the cell type in which it is expressed, and this ambiguity further distinguishes it from other EF-hand CaBPs. In sensory cells, recent studies suggest that OCM plays an essential role in maintaining auditory function, most likely affecting OHC motility mechanisms. In immune cells, OCM may be secreted in response to inflammatory signals and facilitates axon regeneration.