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  4. Recombinant Mouse Nuclear factor 1 A-type (Nfia)

Recombinant Mouse Nuclear factor 1 A-type (Nfia)

  • 货号：
    CSB-YP015754MO
  • 规格：
  • 来源：
    Yeast
  • 其他：
  • 货号：
    CSB-EP015754MO
  • 规格：
  • 来源：
    E.coli
  • 其他：
  • 货号：
    CSB-EP015754MO-B
  • 规格：
  • 来源：
    E.coli
  • 共轭：
    Avi-tag Biotinylated

    E. coli biotin ligase (BirA) is highly specific in covalently attaching biotin to the 15 amino acid AviTag peptide. This recombinant protein was biotinylated in vivo by AviTag-BirA technology, which method is BriA catalyzes amide linkage between the biotin and the specific lysine of the AviTag.

  • 其他：
  • 货号：
    CSB-BP015754MO
  • 规格：
  • 来源：
    Baculovirus
  • 其他：
  • 货号：
    CSB-MP015754MO
  • 规格：
  • 来源：
    Mammalian cell
  • 其他：

产品详情

  • 纯度：
    >85% (SDS-PAGE)
  • 基因名：
    Nfia
  • Uniprot No.：
  • 别名：
    Nfia; Nuclear factor 1 A-type; NF1-A; Nuclear factor 1/A; CCAAT-box-binding transcription factor; CTF; Nuclear factor I/A; NF-I/A; NFI-A; TGGCA-binding protein
  • 种属：
    Mus musculus (Mouse)
  • 蛋白长度：
    Full length protein
  • 表达区域：
    1-532
  • 氨基酸序列
    MKLADSVMAG KASDGSIKWQ LCYDISARTW WMDEFHPFIE ALLPHVRAFA YTWFNLQARK RKYFKKHEKR MSKEEERAVK DELLSEKPEV KQKWASRLLA KLRKDIRPEY REDFVLTVTG KKPPCCVLSN PDQKGKMRRI DCLRQADKVW RLDLVMVILF KGIPLESTDG ERLVKSPQCS NPGLCVQPHH IGVSVKELDL YLAYFVHAAD SSQSESPSQP SEADIKDQPE NGHLGFQDSF VTSGVFSVTE LVRVSQTPIA AGTGPNFSLS DLESSSYYSM SPGAMRRSLP STSSTSSTKR LKSVEDEMDS PGEEPFYTGQ GRSPGSGSQS SGWHEVEPGL PSPSTLKKSE KSGFSSPSPS QTSSLGTAFT QHHRPVITGP RASPHATPST LHFPTSPIIQ QPGPYFSHPA IRYHPQETLK EFVQLVCPDA GQQAGQVGFL NPNGSSQGKV HNPFLPTPML PPPPPPPMAR PVPLPMPDTK PPTTSTEGGA ASPTSPTYST PSTSPANRFV SVGPRDPSFV NIPQQTQSWY LG
  • 蛋白标签：
    Tag type will be determined during the manufacturing process.
    The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.
  • 产品提供形式：
    Lyophilized powder
    Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
  • 复溶：
    We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
  • 储存条件：
    Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
  • 保质期：
    The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
    Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
  • 货期：
    Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
    Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
  • 注意事项：
    Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
  • Datasheet ：
    Please contact us to get it.

产品评价

靶点详情

  • 功能：
    Recognizes and binds the palindromic sequence 5'-TTGGCNNNNNGCCAA-3' present in viral and cellular promoters and in the origin of replication of adenovirus type 2. These proteins are individually capable of activating transcription and replication.
  • 基因功能参考文献：
    1. Of these, NFIA emerged as the regulator yielding the strongest molecular and cellular phenotypes. To examine its regulatory function, we profiled the genomic localization of NFIA, identifying it as a key early regulator of terminal brown fat cell differentiation. PMID: 28181539
    2. The NFIA identifies a subset of bipolar cells as well as Muller glia and astrocytes. During early postnatal development, NFIA labels astrocytes on the day of birth, AII amacrine cells at postnatal (P) day 5, and Muller glia by P10, when horizontal cells also transiently exhibit NFIA immunofluorescence. PMID: 29071714
    3. NFIA expression regulates glioma tumorigenesis and glia-specific enhancers enhancers contribute to glioma formation. PMID: 28892058
    4. NFIA activates the cell-type-specific enhancers and facilitates the binding of PPARgamma to control the brown fat gene program. PMID: 28812581
    5. Dihydrocapsaicin can significantly decrease proinflammatory cytokines through enhancing NFIA and inhibiting NF-kappaB expression PMID: 27267730
    6. Astrocyte induction by Zbtb20 is suppressed by knockdown of NFIA. PMID: 27000654
    7. NFI-A disrupts myeloid cell differentiation and maturation in septic mice PMID: 26259914
    8. NFIA and NFIB are endogenously expressed in gradients with high caudo-medial to low rostro-lateral expression ... this expression pattern deviates from that of PAX6, suggesting that PAX6 does not drive Nfi expression PMID: 26021864
    9. RP5-833A20.1/miR-382-5p/NFIA pathway was essential to the regulation of cholesterol homeostasis and inflammatory reactions. PMID: 25265644
    10. Nfia cooperates with sonic hedgehog signaling to initiate medulloblastoma. PMID: 24167280
    11. Our data thus suggest that NFI nucleosomal interaction may contribute to the partitioning of distinct chromatin domains and to epigenetic gene expression regulation PMID: 23402308
    12. As maturing cerebellar granule neurons become more hyperpolarized, NFATc4 binding declines leading to onset of NFI temporal binding and the NFI switch program PMID: 23407945
    13. NFIA and NFIB are expressed in both the developing and the adult olfactory bulb indicative of a regulatory role for these transcription factors in the development of this facet of the olfactory system. PMID: 22886731
    14. NFIA is expressed in oligodendrocyte progenitors, but not differentiated oligodendrocytes during mouse embryonic development. PMID: 22807310
    15. NFIA is a transcription factor that plays a crucial role in the onset of gliogenesis; we found that its induction is regulated by the transcription factor Sox9 and that this relationship mediates the initiation of gliogenesis PMID: 22500632
    16. The studies demonstrate for the first time a requirement for the NFI-A transcription factor in antigen receptor-induced T-cell activation events. PMID: 21602176
    17. Nfia plays a central role in nervous system development, driving the expression of astrocyte-specific genes but also suppressing the progenitor cell state to allow differentiation to occur. PMID: 20610746
    18. findings suggest that NFI-A, in particular its brain-specific isoform, represses cell adhesion molecule L1 gene expression. PMID: 20003413
    19. Abnormal development of forebrain midline glia and commissural projections in Nfia knock-out mice. PMID: 12514217
    20. nuclear factor I has a role in the intrinsic control of cerebellar granule neuron gene expression PMID: 15466411
    21. brain development, especially oligodendrocyte maturation, is delayed in Nfia-/- mice during the early postnatal period, which at least partly accounts for their phenotype PMID: 17475010
    22. The mouse Nfia mutant phenotype and the common features among five human cases indicate that NFIA haploinsufficiency contributes to a novel human central nervous system malformation syndrome that can also include ureteral and renal defects. PMID: 17530927
    23. new molecular mechanisms controlling osteoclast differentiation and function by the miRNA system and specifically by miRNA-223, which regulates NFI-A and the M-CSFR levels. PMID: 19059913

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  • 亚细胞定位：
    Nucleus.
  • 蛋白家族：
    CTF/NF-I family
  • 数据库链接：

    KEGG: mmu:18027

    STRING: 10090.ENSMUSP00000074899

    UniGene: Mm.31274