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  4. Recombinant Mouse Nibrin (Nbn)

Recombinant Mouse Nibrin (Nbn)

  • 货号：
    CSB-YP015486MO
  • 规格：
  • 来源：
    Yeast
  • 其他：
  • 货号：
    CSB-EP015486MO
  • 规格：
  • 来源：
    E.coli
  • 其他：
  • 货号：
    CSB-EP015486MO-B
  • 规格：
  • 来源：
    E.coli
  • 共轭：
    Avi-tag Biotinylated

    E. coli biotin ligase (BirA) is highly specific in covalently attaching biotin to the 15 amino acid AviTag peptide. This recombinant protein was biotinylated in vivo by AviTag-BirA technology, which method is BriA catalyzes amide linkage between the biotin and the specific lysine of the AviTag.

  • 其他：
  • 货号：
    CSB-BP015486MO
  • 规格：
  • 来源：
    Baculovirus
  • 其他：
  • 货号：
    CSB-MP015486MO
  • 规格：
  • 来源：
    Mammalian cell
  • 其他：

产品详情

  • 纯度：
    >85% (SDS-PAGE)
  • 基因名：
  • Uniprot No.：
  • 别名：
    Nbn; Nbs1; Nibrin; Cell cycle regulatory protein p95; Nijmegen breakage syndrome protein 1 homolog
  • 种属：
    Mus musculus (Mouse)
  • 蛋白长度：
    full length protein
  • 表达区域：
    1-751
  • 氨基酸序列
    MWKLLPAAGA APGEPYRLLA GVEYVVGRKN CGILIENDQS ISRNHAVLTV NFPVTSLSQT DEIPTLTIKD NSKYGTFVNE EKMQTGLSCT LKTGDRVTFG VFESKFRVEY EPLVVCSSCL DVSGKTVLNQ AILQLGGLTA NNWTEECTHL VMSAVKVTIK TICALICGRP IIKPEYFSEF LKAVESKKQP PDIESFYPPI DEPAIGSKSV DLSGRHERKQ IFKGKTFVFL NAKQHKKLSS AVAFGGGEAR LMAEDDEEEQ SFFSAPGTCV VDVGITNTQL IISHSQKKWI HLIMDTLQRN GLRPIPEAEI GLAVIFMTTE NYCNPQGQPC TELKTTTPGP SLSQVLSANG KIIPSAPVNM TTYVADTESE PADTCMPLSE RPEEVKIPGL EQSSRKLSQE TFNIKEAPKP SSKANNVASD TLVRGKTPSY QLSPMKFPVA NKNKDWTSQQ QQNSIKNYFQ PCTRKRERDE DNPELSSCKS SRMELSCSLL EQTQPAGPSL WKSKEHQSQN ATLDREADTS SVGGMDIELN RKSPDRKPLP TETLRPRKRK DVDLATEEEV LEELLRSTKP ELAVQVKVEK QEADDTIRKK PRMDAERNRP LNGGSEPESN SALQEDEREK KDELQTESWS TKHEIANSDG LQDSSEELPR KLLLTEFRSL VVSNHNSTSR NLCVNECGPL KNFKKFKKAT FPGAGKLPHI IGGSDLVGHH ARKNTELEEW LKQEMEVQKQ QAKEESLADD LFRYNPNVKR R
  • 蛋白标签：
    Tag type will be determined during the manufacturing process.
    The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.
  • 产品提供形式：
    Lyophilized powder
    Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
  • 复溶：
    We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
  • 储存条件：
    Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
  • 保质期：
    The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
    Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
  • 货期：
    Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
    Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
  • 注意事项：
    Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
  • Datasheet ：
    Please contact us to get it.

产品评价

靶点详情

  • 功能：
    Component of the MRE11-RAD50-NBN (MRN complex) which plays a critical role in the cellular response to DNA damage and the maintenance of chromosome integrity. The complex is involved in double-strand break (DSB) repair, DNA recombination, maintenance of telomere integrity, cell cycle checkpoint control and meiosis. The complex possesses single-strand endonuclease activity and double-strand-specific 3'-5' exonuclease activity, which are provided by MRE11. RAD50 may be required to bind DNA ends and hold them in close proximity. NBN modulate the DNA damage signal sensing by recruiting PI3/PI4-kinase family members ATM, ATR, and probably DNA-PKcs to the DNA damage sites and activating their functions. It can also recruit MRE11 and RAD50 to the proximity of DSBs by an interaction with the histone H2AX. NBN also functions in telomere length maintenance by generating the 3' overhang which serves as a primer for telomerase dependent telomere elongation. NBN is a major player in the control of intra-S-phase checkpoint and there is some evidence that NBN is involved in G1 and G2 checkpoints. The roles of NBS1/MRN encompass DNA damage sensor, signal transducer, and effector, which enable cells to maintain DNA integrity and genomic stability. Forms a complex with RBBP8 to link DNA double-strand break sensing to resection. Enhances AKT1 phosphorylation possibly by association with the mTORC2 complex.
  • 基因功能参考文献：
    1. Loss of Nbn expression is associated with premature hair loss. PMID: 27050272
    2. the essential role of Nbs1 is via its interaction with Mre11 and that most of the Nbs1 protein is dispensable for Mre11 complex functions and suggest that Mre11 and Rad50 directly activate ATM. PMID: 28076792
    3. Low NBS1 expression is associated with B-cell lymphomas. PMID: 28819025
    4. Phosphorylation status of NBS1 determines how dysfunctional telomeres are repaired. PMID: 28216226
    5. findings show that NBS1 is crucial for macrophage function during normal aging PMID: 26324700
    6. TRIP13-deficient spermatocytes also progress to an H1t-positive stage if ATM activity is attenuated by hypomorphic mutations in Mre11 or Nbs1 or by elimination of the ATM-effector kinase CHK2 PMID: 25768017
    7. In the absence of wild type nibrin, the repair of spontaneous errors, presumably arising during DNA replication, makes a major contribution to the basal mutation rate. PMID: 25771721
    8. Nbs1 mutants initially accumulate replication intermediate, not DSBs. PMID: 24388752
    9. This report showed that ATM-Chk2-P53 signaling pathway and the AKT/mTOR signaling pathway are responsible for the enhanced apoptosis of the Nbn-deficient mature oligodendrocytes. PMID: 24272708
    10. JNK signaling and ATR signaling are likely to converge to regulate the cerebellar apoptosis of newborn Nbn-deficient mice. PMID: 23934213
    11. Nbn and Atm collaborate to prevent DSB accumulation and apoptosis during development in a tissue- and developmental stage-specific manner. PMID: 23935957
    12. the antagonism and redundancy of ATMIN and NBS1 constitute a crucial regulatory mechanism for ATM signaling and function. PMID: 23219553
    13. Nbs1-deficient neocortex shows accumulative endogenous DNA damage and defective activation of Ataxia telangiectasia and Rad3-related (ATR)-Chk1 pathway upon DNA damage. PMID: 22212482
    14. NBS1 haploinsufficiency results in increased mammary tumor latency and metastasis. PMID: 22576691
    15. a distinct function of Nbs1 and Atr in neurogenesis PMID: 22078264
    16. murine cells have evolved mechanisms to ensure the functional redundancy of Pif1 or Nbs1 in the regulation of chromosome healing. PMID: 21945094
    17. Atm has cellular roles not regulated by Nbs1 in the murine cerebellum. PMID: 21300797
    18. deletion of the entire Nbs1 protein in T-cell precursors (Nbs1(T-del)) results in severe lymphopenia and a hindrance to the double-negative 3 (DN3)-to-DN4 transition in early T-cell development PMID: 20921278
    19. Study concludes that Nbs1 and Rad54 function cooperatively, but in separate pathways to counteract DNA double-strand break. PMID: 19782649
    20. MRE11-RAD50-NBS1 complex dictates DNA repair independent of H2AX. PMID: 19910469
    21. Atm and NBS1 function in overlapping as well as distinct pathways in regulating cellular response to DNA damage and animal development. PMID: 11889050
    22. murine model of Nijmegen breakage syndrome, the Nbs1(DeltaB/DeltaB) mouse PMID: 11967151
    23. Gamma-irradiation enhanced tumor development in Nbn(+/-) mice, giving rise to a high frequency of epithelial tumors, mostly in the thyroid and lung, as well as lymphomas. PMID: 14612522
    24. Data show that NBS1, H2AX, and p53 play synergistic roles in ATM-dependent DNA damage responses and tumor suppression PMID: 15632067
    25. Nibrin plays a role in the repair of gamma-irradiation damage, maintenance of chromosomal stability, and the recombination of Ig constant region genes in B lymphocytes. PMID: 15668383
    26. Nbs1 is critical for efficient Ig class-switch recombination and maintenance of the integrity of chromosomal structure and number. PMID: 15668392
    27. Nbs1 is a novel p53-independent Mdm2 binding protein and links Mdm2 to the Mre11-Nbs1-Rad50-regulated DNA repair response PMID: 15734743
    28. Inactivation of the Nbn gene in neural tissues results in a combination of the neurological anomalies characteristic of Nijmegen breakage syndrome, ataxia telangiectasia and ATLD, including microcephaly, growth retardation, cerebellar defects and ataxia. PMID: 15821748
    29. overexpression of NBS1 is an oncogenic event that contributes to transformation through the activation of PI3-kinase/Akt PMID: 16036916
    30. Nbs1 functions in terminal differentiation of the lens fibre cells and in cataractogenesis. PMID: 16790366
    31. Cells lacking Nbs1 display reduced homologous repair of the single double-strand break in chromosomally integrated substrate. PMID: 17082765
    32. the defects observed in Nbs1(DeltaC/DeltaC) result from impaired phosphorylation of ATM targets PMID: 17429352
    33. REVIEW of phosphorylation signaling cascade response to DNA damage.Phosphorylation of NBS1 is regulated by the acetylation status of the protein, which is modulated by SIRT1 deacetylase. PMID: 18156798
    34. The (NBS1 [MRN]) complex accumulates at sites of DNA double-strand breaks (DSBs) in microscopically discernible nuclear foci. PMID: 18411308
    35. Accelerated DNA repair required both the NBS1 protein and Hdm2, accompanied by phosphorylation of Hdm2, dissociation of NBS-1 and Hdm2, inhibition of NBS-1 degradation, and accelerated phosphorylation of ATM. PMID: 18723444
    36. Sequences in mouse nibrin required to direct the nuclear localization of the MRE11/RAD50/nibrin complex were identified by site-specific mutagenesis. PMID: 19075003
    37. During V(D)J recombination, MRN deficiency leads to the aberrant joining of Rag double-strand breaks (DSBs) and to the accumulation of unrepaired coding ends. PMID: 19221393
    38. the NBS1/ATR/BRCA1 repair machinery affects centrosome behavior, and this might be a crucial role in the prevention of malignances. PMID: 19244116
    39. This study demonstrated that Nbs1 loss seriously hampered the organization of glial cells in the optic nerve as well as in the retina PMID: 19345213
    40. NBS1 is required for alternative NHEJ of hairpin coding ends, suppresses alternative NHEJ of signal ends, and promotes proper resolution of inversional recombination intermediates. PMID: 19362533
    41. Results suggest a model in which MRN stabilizes distant breaks and processes DNA termini to facilitate repair by both the classical and alternative NHEJ pathways. PMID: 19633670
    42. The role of Mre11/Rad50/Nbs1 at dysfunctional telomeres is multifaceted, involving both repression of NHEJ in G(2) through end resection and induction of NHEJ in G(1) through ATM-dependent signaling. PMID: 19667071

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  • 亚细胞定位：
    Nucleus. Nucleus, PML body. Chromosome, telomere. Chromosome.
  • 组织特异性：
    High expression in the liver, heart and testis. Low expression in all other tissues analyzed. In the cerebellum the postmitotic Purkinje cells are marked specifically.
  • 数据库链接：

    KEGG: mmu:27354

    STRING: 10090.ENSMUSP00000029879

    UniGene: Mm.20866