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  4. Recombinant Mouse Menin (Men1)

Recombinant Mouse Menin (Men1)

  • 货号：
    CSB-YP013694MO
  • 规格：
  • 来源：
    Yeast
  • 其他：
  • 货号：
    CSB-EP013694MO
  • 规格：
  • 来源：
    E.coli
  • 其他：
  • 货号：
    CSB-EP013694MO-B
  • 规格：
  • 来源：
    E.coli
  • 共轭：
    Avi-tag Biotinylated

    E. coli biotin ligase (BirA) is highly specific in covalently attaching biotin to the 15 amino acid AviTag peptide. This recombinant protein was biotinylated in vivo by AviTag-BirA technology, which method is BriA catalyzes amide linkage between the biotin and the specific lysine of the AviTag.

  • 其他：
  • 货号：
    CSB-BP013694MO
  • 规格：
  • 来源：
    Baculovirus
  • 其他：
  • 货号：
    CSB-MP013694MO
  • 规格：
  • 来源：
    Mammalian cell
  • 其他：

产品详情

  • 纯度：
    >85% (SDS-PAGE)
  • 基因名：
    Men1
  • Uniprot No.：
  • 别名：
    Men1; Menin
  • 种属：
    Mus musculus (Mouse)
  • 蛋白长度：
    Full length protein
  • 表达区域：
    1-611
  • 氨基酸序列
    MGLKAAQKTL FPLRSIDDVV RLFAAELGRE EPDLVLLSLV LGFVEHFLAV NRVIPTNVPE LTFQPSPAPD PPGGLTYFPV ADLSIIAALY ARFTAQIRGA VDLSLYPREG GVSSRELVKK VSDVIWNSLS RSYFKDRAHI QSLFSFITGT KLDSSGVAFA VVGACQALGL RDVHLALSED HAWVVFGPNG EQTAEVTWHG KGNEDRRGQT VNAGVAERSW LYLKGSYMRC DRKMEVAFMV CAINPSIDLH TDSLELLQLQ QKLLWLLYDL GHLERYPMAL GNLADLEELE PTPGRPDPLT LYHKGIASAK TYYQDEHIYP YMYLAGYHCR NRNVREALQA WADTATVIQD YNYCREDEEI YKEFFEVAND VIPNLLKEAA SLLETGEERT GEQAQGTQGQ GSALQDPECF AHLLRFYDGI CKWEEGSPTP VLHVGWATFL VQSLGRFEGQ VRQKVHIVSR EAEAAEAEEP WGDEAREGRR RGPRRESKPE EPPPPKKPAL DKGPGSGQSA GSGPPRKTSG TVPGTTRGGQ EVGNAAQAPA PAASPPPEGP VLTFQSEKMK GMKELLVATK INSSAIKLQL TAQSQVQMKK QKVSTPSDYT LSFLKRQRKG L
  • 蛋白标签：
    Tag type will be determined during the manufacturing process.
    The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.
  • 产品提供形式：
    Lyophilized powder
    Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
  • 复溶：
    We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
  • 储存条件：
    Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
  • 保质期：
    The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
    Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
  • 货期：
    Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
    Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
  • 注意事项：
    Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
  • Datasheet ：
    Please contact us to get it.

产品评价

靶点详情

  • 功能：
    Essential component of a MLL/SET1 histone methyltransferase (HMT) complex, a complex that specifically methylates 'Lys-4' of histone H3 (H3K4). Functions as a transcriptional regulator. Binds to the TERT promoter and represses telomerase expression. Plays a role in TGFB1-mediated inhibition of cell-proliferation, possibly regulating SMAD3 transcriptional activity. Represses JUND-mediated transcriptional activation on AP1 sites, as well as that mediated by NFKB subunit RELA. Positively regulates HOXC8 and HOXC6 gene expression. May be involved in normal hematopoiesis through the activation of HOXA9 expression. May be involved in DNA repair.
  • 基因功能参考文献：
    1. our results suggest that the influence of menin on synapse formation and synaptic plasticity occur via modulation of nAChR channel subunit composition and functional clustering. PMID: 28496137
    2. menin maintains the T-cell functions by limiting mTORC 1 activity and subsequent cellular metabolism. PMID: 30120246
    3. Results showed that the MEN1 gene was negatively correlated with lung fibrinogen markers and provide evidence that MEN1 plays a key role in the formation of pulmonary fibrosis by regulating the secretion of TGF-beta and the activation of TGF-beta/Smads signaling pathway. PMID: 30099020
    4. Men1 - a tumor suppressor gene - is critical for ossifying fibroma (OF) formation. Mice with targeted disruption of Men1 in osteoblasts develop multifocal OF in the mandible with a 100% penetrance. PMID: 28991228
    5. Men1-deficient osteocytes was expressed numerous soluble mediators such as C-X-C motif chemokine 10 (CXCL10 and a novel role for Men1 in osteocyte-osteoclast crosstalk by controlling osteoclastogenesis through the action of soluble factors. PMID: 28106886
    6. Gastrin induces nuclear export and proteasome degradation of menin in duodenal glial cells. PMID: 28859856
    7. gene expression analysis revealed that Menin was involved in the maintenance of the high expression of the previously identified Th2-specific genes rather than the induction of these genes. This result suggests that Menin plays a role in the maintenance of Th2 cell identity.This study confirmed the critical role of Menin in Th2 cell-mediated immune responses. PMID: 28659357
    8. Inhibition of miR-24 increases menin and TGF-beta1 expression, subsequently increasing hepatic fibrosis in FVB/NJ WT and Mdr2(-/-) mice. PMID: 28602220
    9. Menin and PRMT5 suppress GLP1R transcript levels and PKA-mediated phosphorylation of FOXO1 and CREB. PMID: 28270438
    10. Inactivation of Kmt2a in Men1-deficient mice accelerated pancreatic islet tumorigenesis and shortened the average life span. Increases in cell proliferation were observed in mouse pancreatic islet tumors upon inactivation of both Kmt2a and Men1. PMID: 27801610
    11. Data suggest that menin inhibits differentiation into terminal effectors and positively controls proliferation and survival of Ag-specific CD8(+) T cells that are activated upon infection; study uncovered an important role for menin in the immune response of CD8(+) T cells to infection PMID: 27798149
    12. Our data further confirms that deletion of Men1 alone does not favour carcinoid development, but rather cooperates with additional loci. PMID: 26860771
    13. Results indicate that fasted male Men1(+/-) mice, in the early stage of development of MEN1, display glucose metabolic disorders. These disorders are caused not by direct induction of insulin resistance, but via increased glucagon secretion and the consequent stimulation of hepatic glucose production. PMID: 27432891
    14. The inactivation of menin in the thyroid gland of young mice does not seem to change the histological pattern, but it influences the proliferation of follicular cells. Further molecular studies especially in aged mice are needed to better understand the correlation between certain oncogenes and the inactive status of menin. PMID: 27151693
    15. Data show that progranulin is upregulated in neuroendocrine tumors (NETs) and islets of the multiple endocrine neoplasia 1 protein (MEN1) mouse as well as in the serum of patients with pancreatic NETs associated with glucagonoma syndrome. PMID: 26495792
    16. Although mice lacking Men1 developed insulinomas as expected, elimination of ARC in this context did not significantly alter tumor load. Cellular rates of proliferation and death in these tumors were also not perturbed in the absence of ARC. PMID: 26709830
    17. The study characterized the binding position of Ezh2 and menin at all annotated genes in embryonic stem cells and B and T lymphocytes. PMID: 26324324
    18. Data demonstrate that loss of Men1 in pancreatic islet cells alters the epigenetic landscape of its target genes during early stages of pancreatic neuroendocrine tumor formation. PMID: 25537453
    19. Data show that menin, encoded by the MEN1 gene, inhibits the transcriptional activity of nuclear receptor liver X receptor alpha (LXRalpha). PMID: 25962847
    20. Conditional knockout of beta-catenin suppresses the tumorigenesis and growth of Men1-deficient pancreatic neuroendocrine tumors. PMID: 25517963
    21. miR-802 expression levels are increased in lung carcinoma tissues and targets the tumor suppressor menin and downregulates its expression. PMID: 24994111
    22. CDK4 is a critical downstream target of MEN1-dependent tumor suppression and is required for tumorigenic proliferation in the pituitary and pancreatic islet, whereas CDK2 is dispensable for tumorigenesis in these neuroendocrine cell types. PMID: 24531709
    23. This work shows that Men1 ablation in the pancreatic beta-cells leads to the inactivation of specific transcription factors, resulting in glucagon-expressing tumor development, which sheds light on the mechanisms of islet tumorigenesis. PMID: 25343275
    24. Menin regulates spinal glutamate-GABA balance through GAD65 contributing to neuropathic pain. PMID: 24905306
    25. Menin orchestrates Th17 cell differentiation and function by regulating both the induction and maintenance of target gene expression. PMID: 25136117
    26. Menin recruits SIRT1 to control hepatic CD36 expression and triglyceride accumulation through histone deacetylation PMID: 23867312
    27. Menin binding at the Bach2 locus and the Bach2 expression are decreased in the senescent CD4 T cells. PMID: 24694524
    28. Loss of MEN1 expression is associated with insulinoma. PMID: 24157940
    29. a novel mechanism whereby menin suppresses cell proliferation, at least partly by promoting the processing of certain miRNAs, including let-7a, leading to suppression of Irs2 expression and insulin signaling. PMID: 24563463
    30. Findings uncover a novel epigenetic link whereby menin directly represses Gli1 expression, independent of the canonical Hedgehog signaling pathway. PMID: 23928057
    31. Data indicate that the Menin-Yap1 axis responded to CCl4-induced liver inflammation. PMID: 24101467
    32. The trithorax protein partner menin acts in tandem with EZH2 to suppress C/EBPalpha and differentiation in MLL-AF9 leukemia. PMID: 23349306
    33. menin and MLL1 regulate distinct pathways during normal hematopoiesis, particularly in HSCs and B cells. PMID: 23908472
    34. The results of this study found e role of upregulated neuronal menin in the spinal cord in potentiating spinal synaptic plasticity in peripheral nerve injury-induced neuropathic hypersensitivity. PMID: 22858595
    35. Menin may inhibit MEN1 insulinoma by suppressing cyclin B2 expression via histone modification. PMID: 22193451
    36. Simultaneous heterozygous deletion of Men1 in animals with either heterozygous or homozygous deletion of Trp53 did not result in formation of tumours at any new sites. PMID: 22708734
    37. Findings indicate that MEN1 tumor suppressor gene replacement therapy can generate menin expression in pituitary tumors, and significantly reduce tumor cell proliferation. PMID: 22915754
    38. Epithelium-specific deletion of the multiple endocrine neoplasia type 1 locus resulted in hypergastrinemia due to antral G cell hyperplasia and a hyperproliferative epithelium, but no gastrinomas. PMID: 22766853
    39. a possible role for menin-dependent H3K4me3 at these genes in the initiation and progression of sporadic pancreatic endocrine tumors PMID: 22666422
    40. Data show that numerous genes were differentially expressed in the endocrine pancreas of 5-wk-old heterozygous multiple endocrine neoplasia 1 protein (Men1) mice. PMID: 22492302
    41. menin could induce endodermal differentiation of uncommitted cells by functionally modulating the RARs. PMID: 21833538
    42. findings show that the MEN1 gene suppresses malignant phenotypes of melanoma cells through multiple signalling pathways PMID: 21129151
    43. Menin expression is regulated by transforming growth factor beta signaling in leukemia cells. PMID: 21740816
    44. menin acts synergistically with the nuclear receptor PPARalpha to control gene expression of fatty acid oxidation PMID: 22001208
    45. Embryoid bodies formed from null mutant (Men1-/-) and wild-type (Men1+/+) embryonic stem cells were used as a model system to investigate the effects of the Men1 gene on pancreatic islet development. PMID: 21468568
    46. Menin interacts with beta-catenin and facilitates differentiation of osteoblast. PMID: 21264795
    47. menin regulates H3K27me3 and promoter DNA methylation via WT1, which plays an important role in regulating the function of the menin/PcG axis, H3K27 methylation, and DNA methylation, resulting in repression of gene transcription PMID: 21378168
    48. It was concluded that during pregnancy, placental hormones act through the prolactin receptor to increase beta-cell mass by up regulating beta-cell proliferation by engaging Jak2, Akt, menin/p18, and p21. PMID: 21239436
    49. Confocal microscopy analysis revealed that menin regulates AKT1 in part by reducing the translocation of AKT1 from the cytoplasm to the plasma membrane. PMID: 21127195
    50. Loss of Men1 is associated with prostate cancer lesions. PMID: 20663219

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  • 亚细胞定位：
    Nucleus.
  • 组织特异性：
    Widely expressed, with high levels in hippocampus, cerebral cortex, testis and thymus (at protein level). Also expressed at high levels in pancreatic islets, ovary and bone marrow. In the brain, highest expression in hippocampus pyramidal nerve cells (at
  • 数据库链接：

    KEGG: mmu:17283

    STRING: 10090.ENSMUSP00000109130

    UniGene: Mm.12917