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  4. Recombinant Mouse LIM homeobox transcription factor 1-beta (Lmx1b)

Recombinant Mouse LIM homeobox transcription factor 1-beta (Lmx1b)

  • 货号：
    CSB-YP013019MO
  • 规格：
  • 来源：
    Yeast
  • 其他：
  • 货号：
    CSB-EP013019MO
  • 规格：
  • 来源：
    E.coli
  • 其他：
  • 货号：
    CSB-EP013019MO-B
  • 规格：
  • 来源：
    E.coli
  • 共轭：
    Avi-tag Biotinylated

    E. coli biotin ligase (BirA) is highly specific in covalently attaching biotin to the 15 amino acid AviTag peptide. This recombinant protein was biotinylated in vivo by AviTag-BirA technology, which method is BriA catalyzes amide linkage between the biotin and the specific lysine of the AviTag.

  • 其他：
  • 货号：
    CSB-BP013019MO
  • 规格：
  • 来源：
    Baculovirus
  • 其他：
  • 货号：
    CSB-MP013019MO
  • 规格：
  • 来源：
    Mammalian cell
  • 其他：

产品详情

  • 纯度：
    >85% (SDS-PAGE)
  • 基因名：
    Lmx1b
  • Uniprot No.：
  • 别名：
    Lmx1b; LIM homeobox transcription factor 1-beta; LIM/homeobox protein 1.2; LMX-1.2; LIM/homeobox protein LMX1B
  • 种属：
    Mus musculus (Mouse)
  • 蛋白长度：
    Full length protein
  • 表达区域：
    1-395
  • 氨基酸序列
    MDIATGPESL ERCFPRGQTD CAKMLDGIKM EEHALRPGPA TLGVLLGSDC PHPAVCEGCQ RPISDRFLMR VNESSWHEEC LQCAACQQAL TTSCYFRDRK LYCKQDYQQL FAAKCSGCME KIAPTEFVMR ALECVYHLGC FCCCVCERQL RKGDEFVLKE GQLLCKGDYE KEKDLLSSVS PDESDSVKSE DEDGDMKPAK GQGSQSKGSG DDGKDPRRPK RPRTILTTQQ RRAFKASFEV SSKPCRKVRE TLAAETGLSV RVVQVWFQNQ RAKMKKLARR HQQQQEQQNS QRLGQEVLSS RMEGMMASYT PLAPPQQQIV AMEQSPYGSS DPFQQGLTPP QMPGNDSIFH DIDSDTSLTS LSDCFLGSSD VGSLQARVGN PIDRLYSMQS SYFAS
  • 蛋白标签：
    Tag type will be determined during the manufacturing process.
    The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.
  • 产品提供形式：
    Lyophilized powder
    Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
  • 复溶：
    We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
  • 储存条件：
    Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
  • 保质期：
    The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
    Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
  • 货期：
    Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
    Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
  • 注意事项：
    Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
  • Datasheet ：
    Please contact us to get it.

产品评价

靶点详情

  • 功能：
    Essential for the specification of dorsal limb fate at both the zeugopodal and autopodal levels.
  • 基因功能参考文献：
    1. s conclude that Lmx1b plays a key role as an anti-osteogenic factor in patterning the head mesenchyme into areas with different osteogenic competence. In turn, this patterning event is crucial to generating the proper organization of the bones and soft tissue joints of the calvaria. PMID: 29852132
    2. Data show that sustained expression of Lmx1a and Lmx1b is required for the survival of adult midbrain dopaminergic neurons. PMID: 27407143
    3. Lmx1b is required for the topographical organization of dopaminergic innervation in the striatum.Lmx1b regulates Plxnc1 expression. PMID: 29038581
    4. This is the first report to describe genome-wide Lmx1b binding during limb development. PMID: 28455377
    5. Otx2 critically depends on Lmx1b for the formation of mdDA neurons. PMID: 26444681
    6. results demonstrate an important role for the intersection of Lmx1b and Hoxb8::cre expression in the development of nociceptive dorsal horn circuits critical for mechanical and thermal pain processing. PMID: 25834049
    7. Co-immunoprecipitation experiments show that both wild-type and Icst LMX1B are found in complexes with LIM domain binding protein 1 (LDB1), resulting in lower levels of functional LMX1B in Icst heterozygotes than null heterozygotes PMID: 24809698
    8. We posit that microRNA modulation of the Lmx1b/Wnt axis in the early midbrain/isthmus could determine midbrain size and allocation of dopamine progenitors. PMID: 24348261
    9. Cell biological and biophysical experiments with primary podocytes isolated after 1 week of Lmx1b inactivation indicated dysregulation of actin cytoskeleton organization PMID: 23990680
    10. Lmx1b, a key transcription factor for the specification of 5-HT and dopaminergic transmitter phenotypes during embryogenesis, determines some peptide phenotypes in these neurons as well. PMID: 23532063
    11. Data indicate that tryptophan hydroxylase 2 Tph2-/- and LIM homeobox transcription factor 1 beta Lmx1b-/- females displayed a change in sexual preference. PMID: 23716677
    12. A protein complex was identified containing PSPC1, PSF and LMX1B, suggesting the existence of this complex in mesodiencephalic dopaminergic neurons. PMID: 23308148
    13. Lmx1b genes are associated with skeletal development. PMID: 22417325
    14. This study demonistrated that Lmx1b promotes a neuronal glutamate phenotype and suppresses a GABA one in the embryonic trigeminal brainstem complex PMID: 22397680
    15. This study demonstrated that Lmx1a and Lmx1b function cooperatively to regulate proliferation, specification, and differentiation of mDA progenitors, including their floor plate-like properties. PMID: 21880902
    16. data establish that Lmx1b influences the differentiation of multiple neuronal subtypes in the ventral midbrain, whereas Lmx1a appears to be exclusively devoted to the differentiation of the dopamine neuron lineage PMID: 21752929
    17. report 14 genes that require Lmx1b for their normal expression in the dorsal limb or the restriction of their expression to the ventral limb. PMID: 20589901
    18. link a specific transcription factor Lmx1b to trabecular meshwork formation and demonstrate specific requirements for Lmx1b in maintaining the integrity of adult anterior segment. PMID: 20568247
    19. In Lmx1b knockout ((-)(/)(-)) mice, V primary afferent projections to PrV are normal, albeit reduced in number, whereas the PrV-thalamic lemniscal pathway is sparse and develops late. PMID: 20621716
    20. obtained evidence that Lmx1a and Lmx1b cooperate with Foxa2 to specify mesDA neuron identity by gain-of-function approaches using transgenic mice PMID: 20035737
    21. Results indicate that whereas Lmx1b activity is required autonomously in skeletal progenitors to direct dorsal pattern, loss or gain of Lmx1b activity in skeletal progenitors has no effect on muscle or connective tissue patterning. PMID: 20215352
    22. When Lmx1b expression is deleted at the dorsal midline, Lmx1b/Lmx1a-null mutants fail to generate a hindbrain roof plate. PMID: 19741143
    23. Transcriptional induction of slit diaphragm genes by Lmx1b is required in podocyte differentiation. PMID: 11956244
    24. The LIM-homeodomain transcription factor Lmx1b plays a crucial role in podocytes PMID: 11956245
    25. Review. Studies of Lmx1b knockout mice show it is a transactivator required for coordinate expression of Col4a3 and Col4a4, and other podocyte function genes. PMID: 11978876
    26. During development of 5-HT neurons, Lmx1b is a critical intermediate factor that couples early specification with nerve terminal differentiation. PMID: 12897786
    27. Ectopic expression of Lmx1b plus Pet-1 is able to induce formation of 5-HT cells in the most ventral spinal cord, where Nkx2.2 is normally expressed. Combined expression of Lmx1b, Pet-1, and Nkx2.2, drives 5-HT differentiation in the dorsal spinal cord PMID: 14602809
    28. Myogenic precursors in limb bud were devoid of Lmx1b expression. Lmx1b is not only expressed at early stages of limb development but maintains its pattern, at least until after birth. New details of Lmx1b expression on cellular basis within limb. PMID: 15007643
    29. Lmx1b expression localized to dorsal joint-forming regions, to developing tendons and ligaments PMID: 15183306
    30. data identify Lmx1b as a key regulator of spinal cord roof plate induction and function PMID: 15215291
    31. Results indicate that Lmx1b has a pivotal role in genetic cascades that control the assembly of circuitry in the superficial dorsal horn. PMID: 15229182
    32. Lmx1b is required for the differentiation and migration of neurons within the dorsal spinal cord. The inability of afferent sensory neurons to migrate into the dorsal horn is entirely consistent with diminished pain responses in nail patella syndrome PMID: 15562281
    33. A critical role is revealed for Lmx1b in maintaining the differentiated status of serotonergic (5-HT) neurons in conditional knock-out Lmx1bf/f/p mice compared with wild-type mice. PMID: 17151281
    34. Lmx1b plays an essential role in the development of the tectum and cerebellum by regulating expression of Fgf8, Wnt1 and several isthmus-related transcription factors in the midbrain-hindbrain boundary PMID: 17166916
    35. The podocyte-specific inactivation of Lmx1b, Ldb1 and E2a yields new insight into a transcriptional network in podocytes PMID: 17316599
    36. First report of a phenotype in heterozygous Lmx1b (Lmx1b+/-) knockout animals. PMID: 17657578
    37. Results suggest that LMX1B controls different targets along the proximal-distal axis of the limb, and suggest the existence of a dorsal proximal limb region that is rich in mRNAs requiring Lmx1b for their expression. PMID: 18351676
    38. Lmx1b is involved in the postnatal maturation of certain types of neurons and maintenance of their normal functions in the adult brain. PMID: 18512225
    39. expression of shRNA against GPM6A markedly reduced the expression of neuroectodermal-associated genes (OTX1, Lmx1b, En1, Pax2, Pax5, Sox1, Sox2, and Wnt1), and also the number of neural stem cells (NSC) derived from D3mshM6A cells PMID: 18522499
    40. These data demonstrate for the first time that LMX1B directly regulates transcription of a subset of NF-kappaB target genes in cooperation with nuclear p50/p65 NF-kappaB. PMID: 18996370
    41. Foxa1 and Foxa2 function both upstream of and cooperatively with Lmx1a and Lmx1b in a feedforward loop promoting mesodiencephalic dopaminergic neuron development PMID: 19607821

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  • 亚细胞定位：
    Nucleus.
  • 数据库链接：

    KEGG: mmu:16917

    STRING: 10090.ENSMUSP00000043616

    UniGene: Mm.39825