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  4. Recombinant Mouse Isocitrate dehydrogenase [NADP] Cytoplasmic domain (Idh1), partial

Recombinant Mouse Isocitrate dehydrogenase [NADP] Cytoplasmic domain (Idh1), partial

  • 货号：
    CSB-EP010989MO
  • 规格：
    ￥1836
  • 图片：
    • (Tris-Glycine gel) Discontinuous SDS-PAGE (reduced) with 5% enrichment gel and 15% separation gel.
  • 其他：

产品详情

  • 纯度：
    Greater than 90% as determined by SDS-PAGE.
  • 基因名：
  • Uniprot No.：
  • 别名：
    Idh1; Isocitrate dehydrogenase [NADP] cytoplasmic; IDH; EC 1.1.1.42; Cytosolic NADP-isocitrate dehydrogenase; IDP; NADP(+)-specific ICDH; Oxalosuccinate decarboxylase
  • 种属：
    Mus musculus (Mouse)
  • 蛋白长度：
    Partial
  • 来源：
    E.coli
  • 分子量：
    49.2kDa
  • 表达区域：
    7-407aa
  • 氨基酸序列
    GGSVVEMQGDEMTRIIWELIKEKLILPYVELDLHSYDLGIENRDATNDQVTKDAAEAIKKYNVGVKCATITPDEKRVEEFKLKQMWKSPNGTIRNILGGTVFREAIICKNIPRLVTGWVKPIIIGRHAYGDQYRATDFVVPGPGKVEITYTPKDGTQKVTYMVHDFEEGGGVAMGMYNQDKSIEDFAHSSFQMALSKGWPLYLSTKNTILKKYDGRFKDIFQEIYDKKYKSQFEAQKICYEHRLIDDMVAQAMKSEGGFIWACKNYDGDVQSDSVAQGYGSLGMMTSVLICPDGKTVEAEAAHGTVTRHYRMYQKGQETSTNPIASIFAWSRGLAHRAKLDNNTELSFFAKALEDVCIETIEAGFMTKDLAACIKGLPNVQRSDYLNTFEFMDKLGENLKA
    Note: The complete sequence including tag sequence, target protein sequence and linker sequence could be provided upon request.
  • 蛋白标签：
    N-terminal 6xHis-tagged
  • 产品提供形式：
    Liquid or Lyophilized powder
    Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
  • 缓冲液：
    Tris-based buffer,50% glycerol
  • 储存条件：
    Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
  • 保质期：
    The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
    Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
  • 货期：
    Basically, we can dispatch the products out in 1-3 working days after receiving your orders. Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
    Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
  • 注意事项：
    Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
  • Datasheet & COA：
    Please contact us to get it.

产品评价

靶点详情

  • 基因功能参考文献：
    1. Vitamin C treatment induced an IDH1(R132H)-dependent reduction in cell proliferation. PMID: 28663574
    2. IDH1 expression regulates TCA cycle in glioma and affects glioma cell growth.GDH2 promotes growth of IDH1-expressing gliomas. PMID: 29097607
    3. Through the use of a conditional mutant mouse model that confers a less aggressive tumor phenotype, findings provide new insight into the effects of mutant Idh1 on a candidate cell of origin for glioma, mutant Idh1's role in disrupting the microenvironment from which gliomas arise, and mutant Idh1's effect on the course of glioma progression. PMID: 28148827
    4. IDH1 mutations caused down-regulation of leukocyte chemotaxis, resulting in repression of the tumor-associated immune system. PMID: 28465358
    5. Idh1(R132H) mutation in the major adult neurogenic stem cell niche causes a phenotype resembling gliomagenesis PMID: 27693047
    6. Using whole RNA sequencing of bone marrow cells in iron-overloaded mice, it was observed that Idh1 and Aco1, enzymes involved in the TCA cycle, were elevated. PMID: 27380194
    7. data suggest that mutant IDH1 contributes to malignancy in the T-cell lineage and may alter the metabolic profile of malignant T cells PMID: 26787889
    8. The results suggest that Idh1 has a physiological function in protecting cells from oxidative stress by regulating the intracellular NADP(+)/NADPH ratio. PMID: 25882048
    9. These results support the translational potential of immunotherapeutic targeting of gliomas carrying IDH1 mutations R132H. PMID: 25849072
    10. MiR-181a regulates lipid metabolism via IDH1 PMID: 25739786
    11. revealed a role for IDH1 in the synthesis/turnover of phospholipids in developing astrocytes and highlight the lipid alterations resulting from the loss of wild-type IDH1 activity. PMID: 25218477
    12. these data show that mutant IDH or d-2HG causes persistence of chondrocytes, giving rise to rests of growth-plate cells that persist in the bone as enchondromas. PMID: 25730874
    13. WA might exert its chemopreventive activity via inhibiting not only oncogenic activation, but also IDH1 inactivation. PMID: 23107437
    14. Brain-specific Idh1-KI mice show brain hemorrhage accompanied by high D2HG levels but decreased ROS PMID: 22925884
    15. Suggest that decreased IDPc expression renders melanocytes more vulnerable to oxidative stress, and IDPc plays an important antioxidant function in melanocytes. PMID: 22264756
    16. Absence of IDH1 mutations in low-grade gliomas (LGGs) identifies a novel entity of LGGs with distinctive location, infiltrative behavior, specific molecular alterations, and dismal outcome. PMID: 21080178
    17. a possible association between IDH mutations and trisomy 8 in myelodysplastic syndromes and acute myeloid leukemia. PMID: 20861910
    18. IDPc would be a major NADPH producer required for fat and cholesterol synthesis. PMID: 15254034
    19. The results suggest that IDPc plays an important protective role in apoptosis of HL-60 cells induced by singlet oxygen. PMID: 17646934
    20. IDPc is expressed differentially along tubules in patterns that may contribute to differences in susceptibility to injury, major enzyme in cytosolic NADPH generation in kidney, downregulated with ischemia-reperfusion PMID: 19106211

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  • 亚细胞定位：
    Cytoplasm, cytosol.
  • 蛋白家族：
    Isocitrate and isopropylmalate dehydrogenases family
  • 数据库链接：

    KEGG: mmu:15926

    STRING: 10090.ENSMUSP00000095316

    UniGene: Mm.9925