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  4. Recombinant Mouse Homeobox protein SIX2 (Six2)

Recombinant Mouse Homeobox protein SIX2 (Six2)

  • 货号：
    CSB-YP730782MO
  • 规格：
  • 来源：
    Yeast
  • 其他：
  • 货号：
    CSB-EP730782MO
  • 规格：
  • 来源：
    E.coli
  • 其他：
  • 货号：
    CSB-EP730782MO-B
  • 规格：
  • 来源：
    E.coli
  • 共轭：
    Avi-tag Biotinylated

    E. coli biotin ligase (BirA) is highly specific in covalently attaching biotin to the 15 amino acid AviTag peptide. This recombinant protein was biotinylated in vivo by AviTag-BirA technology, which method is BriA catalyzes amide linkage between the biotin and the specific lysine of the AviTag.

  • 其他：
  • 货号：
    CSB-BP730782MO
  • 规格：
  • 来源：
    Baculovirus
  • 其他：
  • 货号：
    CSB-MP730782MO
  • 规格：
  • 来源：
    Mammalian cell
  • 其他：

产品详情

  • 纯度：
    >85% (SDS-PAGE)
  • 基因名：
    Six2
  • Uniprot No.：
  • 别名：
    Six2; Homeobox protein SIX2; Sine oculis homeobox homolog 2
  • 种属：
    Mus musculus (Mouse)
  • 蛋白长度：
    full length protein
  • 表达区域：
    1-296
  • 氨基酸序列
    MSMLPTFGFT QEQVACVCEV LQQGGNIERL GRFLWSLPAC EHLHKNESVL KAKAVVAFHR GNFRELYKIL ESHQFSPHNH AKLQQLWLKA HYIEAEKLRG RPLGAVGKYR VRRKFPLPRS IWDGEETSYC FKEKSRSVLR EWYAHNPYPS PREKRELAEA TGLTTTQVSN WFKNRRQRDR AAEAKERENS ENSNSSSHNP LASSLNGSGK SVLGSSEDEK TPSGTPDHSS SSPALLLSPP PPPGLPSLHS LGHPPGPSAV PVPVPGGGGA DPLQHHHSLQ DSILNPMSAN LVDLGS
  • 蛋白标签：
    Tag type will be determined during the manufacturing process.
    The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.
  • 产品提供形式：
    Lyophilized powder
    Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
  • 复溶：
    We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
  • 储存条件：
    Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
  • 保质期：
    The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
    Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
  • 货期：
    Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
    Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
  • 注意事项：
    Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
  • Datasheet ：
    Please contact us to get it.

产品评价

靶点详情

  • 功能：
    Transcription factor that plays an important role in the development of several organs, including kidney, skull and stomach. During kidney development, maintains cap mesenchyme multipotent nephron progenitor cells in an undifferentiated state by opposing the inductive signals emanating from the ureteric bud and cooperates with WNT9B to promote renewing progenitor cells proliferation. Acts through its interaction with TCF7L2 and OSR1 in a canonical Wnt signaling independent manner preventing transcription of differentiation genes in cap mesenchyme such as WNT4. Also acts independently of OSR1 to activate expression of many cap mesenchyme genes, including itself, GDNF and OSR1. During craniofacial development plays a role in growth and elongation of the cranial base through regulation of chondrocyte differentiation. During stomach organogenesis, controls pyloric sphincter formation and mucosal growth through regulation of a gene network including NKX2-5, BMPR1B, BMP4, SOX9 and GREM1. During branchial arch development, acts to mediate HOXA2 control over the insulin-like growth factor pathway. Also may be involved in limb tendon and ligament development. Plays a role in cell proliferation and migration.
  • 基因功能参考文献：
    1. Reveal a unique dose response of nephron progenitors to the level of SIX2 protein in which the role of SIX2 in progenitor proliferation versus self-renewal is separable. PMID: 29217079
    2. Embryonic day 18.5 Six2Frs2alphaKO kidneys were hypoplastic and not cystic, postnatal day (P) 7 mutants had proximal tubular-derived cysts that nearly replaced the renal parenchyma by P21. Mutants had high proximal tubular proliferation rates and interstitial fibrosis, similar to known polycystic kidney disease models. PMID: 27853247
    3. GATA1 may be a potential regulator of Six2-maintained population of nephron progenitor cells. PMID: 28842839
    4. Six2 mediates the protective effects of GDNF on damaged DA neurons by regulating Smurf1 expression. PMID: 27148690
    5. Differentiation of nephron progenitors requires downregulation of Six2, a transcription factor required for progenitor maintenance, and that Notch signaling is necessary and sufficient for Six2 downregulation. PMID: 27633993
    6. Zeb1 promotes proliferation and apoptosis and inhibits the migration of metanephric mesenchyme cells, in association with Six2. PMID: 27509493
    7. In Six2-positive nephrogenic progenitors, GLI3 repressor decreased progenitor cell proliferation reducing the number of nephrogenic precursor structures in the Pallister-Hall syndrome mouse model. PMID: 26604140
    8. Mechanistically, LIF activates STAT, which binds to a Stat consensus sequence in the Six2 proximal promoter and sustains SIX2 levels. PMID: 26321142
    9. Dicer ablation in the early metanephric mesenchyme results in severe renal dysgenesis despite normal initial specification of nephron progenitors and ureteric bud outgrowth. PMID: 24500693
    10. miR181c downregulates the expression of Six2, restrain the proliferation and promote the apoptosis that even makes the nephron progenitor phenotype lose MM cells, suggesting a potential role of miR181c during the kidney development. PMID: 25187057
    11. results reveal a functional link between Eya1, Six2, and Myc in driving the expansion and maintenance of the multipotent progenitors during nephrogenesis PMID: 25458011
    12. Our findings establish Six2 as a regulator of metastasis in human breast cancers and demonstrate an epigenetic function for SIX family transcription factors in metastatic progression through the regulation of E-cadherin. PMID: 25348955
    13. These results implied that inhibition of Nf1 may delay metanephros development via down-regulation of Six2. PMID: 24573885
    14. Osr1 plays crucial roles in Six2-dependent maintenance of nephron progenitors during mammalian nephrogenesis. PMID: 24598167
    15. this study concluded that by targeting the transcription factor gene Six2, miR-181b inhibits the proliferation of metanephric mesenchymal cells in vitro and might play an important role in the formation of nephrons. PMID: 24055707
    16. Wnt and BMP signaling cooperate with Hox in the control of Six2 expression in limb tendon precursor. PMID: 23499659
    17. The Six2 and Lef/Tcf factors form a regulatory complex that promotes progenitor maintenance while entry of beta-catenin into this complex promotes nephrogenesis. PMID: 22902740
    18. Results demonstrate impaired osmoregulatory mechanisms consistent with chronic renal failure in the Br/+ mouse and indicate that six2 haploinsufficiency has a direct effect on postnatal fluid and electrolyte handling associated with fluid imbalance. PMID: 21653879
    19. wnt9b signaling regulates stem cell proliferation and differention in the developing kidney depending on the activity of the transcription factor Six2 in the responding cell. PMID: 21350016
    20. Six4 and Six5 repressed, while Six1 activated myogenin expression, suggesting that the differential regulation of myogenin expression is responsible for the differential effects of Six genes PMID: 20696153
    21. Inactivation of Six2 in mouse identifies a novel genetic mechanism controlling development and growth of the cranial base. PMID: 20515681
    22. Notch2 activation did not skew the cell fate toward the proximal nephron but resulted in severe kidney dysgenesis and depletion of Six2-positive progenitors. PMID: 20299358
    23. Six2 activates Gdnf expression PMID: 15327782
    24. Hoxa2 regulation of Six2 is confined to a 0.9 kb fragment of the Six2 promoter and that Hoxa2 binds to this promoter region PMID: 15634706
    25. our results suggest a cell-intrinsic role of Six2 in the survival of early-generated trigeminal sensory neurons PMID: 16938278
    26. in the developing kidney, Six2 activity is required for maintaining the mesenchymal progenitor population in an undifferentiated state PMID: 17036046
    27. Six2 and Six6 differ in their DNA-binding mechanisms and in the consequences of their interaction with protein tyrosine phosphatase Eyes Absent in Six2/6-DNA-binding ability. PMID: 18293925
    28. propose that Six2 acts to mediate Hoxa2 control over the insulin-like growth factor pathway during branchial arch development PMID: 18321982
    29. Data suggest a mutation in a novel cis-acting regulatory region inhibits Six2 expression and is associated with frontonasal dysplasia and renal hypoplasia. PMID: 18570229
    30. Defects are associated with embryological deficiencies of Six2, suggesting that proper levels of this protein during nephrogenesis are critical for normal glomerular development and adult renal function PMID: 19193724
    31. Six2 activity regulates a gene network which includes Bmp4, Bmpr1b, Nkx2.5, Sox9, & Gremlin during the genesis of the pyloric sphincter in the mouse. It maintains a Bmp4-free PS territory for proper morphogenesis of the PS in the mouse stomach. PMID: 19660448
    32. Data demonstrate that a single enhancer site upstream of the Six2 coding sequence is responsible for both activation by Hox11 proteins in the kidney and repression by Hoxa2 in the branchial arch and facial mesenchyme in vivo. PMID: 19716816

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  • 亚细胞定位：
    Nucleus.
  • 蛋白家族：
    SIX/Sine oculis homeobox family
  • 组织特异性：
    Expressed in phalangeal tendons, in smooth muscle and in head and body mesenchyme.
  • 数据库链接：

    KEGG: mmu:20472

    STRING: 10090.ENSMUSP00000024947

    UniGene: Mm.5039