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  4. Recombinant Mouse Hepatocyte nuclear factor 6 (Onecut1)

Recombinant Mouse Hepatocyte nuclear factor 6 (Onecut1)

  • 货号：
    CSB-YP016336MO
  • 规格：
  • 来源：
    Yeast
  • 其他：
  • 货号：
    CSB-EP016336MO
  • 规格：
  • 来源：
    E.coli
  • 其他：
  • 货号：
    CSB-EP016336MO-B
  • 规格：
  • 来源：
    E.coli
  • 共轭：
    Avi-tag Biotinylated

    E. coli biotin ligase (BirA) is highly specific in covalently attaching biotin to the 15 amino acid AviTag peptide. This recombinant protein was biotinylated in vivo by AviTag-BirA technology, which method is BriA catalyzes amide linkage between the biotin and the specific lysine of the AviTag.

  • 其他：
  • 货号：
    CSB-BP016336MO
  • 规格：
  • 来源：
    Baculovirus
  • 其他：
  • 货号：
    CSB-MP016336MO
  • 规格：
  • 来源：
    Mammalian cell
  • 其他：

产品详情

  • 纯度：
    >85% (SDS-PAGE)
  • 基因名：
  • Uniprot No.：
  • 别名：
    Onecut1; Hnf6; Hnf6a; Hepatocyte nuclear factor 6; HNF-6; One cut domain family member 1; One cut homeobox 1
  • 种属：
    Mus musculus (Mouse)
  • 蛋白长度：
    Full length protein
  • 表达区域：
    1-465
  • 氨基酸序列
    MNAQLTMEAI GELHGVSHEP VPAPADLLGG SPHARSSVGH RGSHLPPAHP RSMGMASLLD GGSGGSDYHH HHRAPEHSLA GPLHPTMTMA CETPPGMSMP TTYTTLTPLQ PLPPISTVSD KFPHHHHHHH HHHHPHHHQR LAGNVSGSFT LMRDERGLAS MNNLYTPYHK DVAGMGQSLS PLSGSGLGSI HNSQQGLPHY AHPGAAMPTD KMLTPNGFEA HHPAMLGRHG EQHLTPTSAG MVPINGLPPH HPHAHLNAQG HGQLLGTARE PNPSVTGAQV SNGSNSGQME EINTKEVAQR ITTELKRYSI PQAIFAQRVL CRSQGTLSDL LRNPKPWSKL KSGRETFRRM WKWLQEPEFQ RMSALRLAAC KRKEQEHGKD RGNTPKKPRL VFTDVQRRTL HAIFKENKRP SKELQITISQ QLGLELSTVS NFFMNARRRS LDKWQDEGGS NSGSSSSSSS TCTKA
  • 蛋白标签：
    Tag type will be determined during the manufacturing process.
    The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.
  • 产品提供形式：
    Lyophilized powder
    Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
  • 复溶：
    We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
  • 储存条件：
    Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
  • 保质期：
    The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
    Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
  • 货期：
    Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
    Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
  • 注意事项：
    Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
  • Datasheet ：
    Please contact us to get it.

产品评价

靶点详情

  • 功能：
    Transcriptional activator. Binds the consensus sequence 5'-DHWATTGAYTWWD-3' on a variety of gene promoters such as those of HNF3B and TTR. Important for liver genes transcription. Stimulates the expression of Onecut3 in the developing endoderm.
  • 基因功能参考文献：
    1. These results suggest that Oc1 and Pdx1 cooperate prior to their divergence, in pancreatic progenitors, to allow for proper differentiation and functional maturation of beta cells. PMID: 27292642
    2. Expression of motor neuron effector genes is maintained by transient enhancers bound by Isl1/Lhx3 in nascent motor neurons and Isl1/Onecut1 in maturing hypaxial motor neurons. PMID: 27939581
    3. Data show that growth hormone (GH)/lipopolysaccharides (LPS) co-treatment enhanced hepatocyte nuclear factor 6 (Hnf6) expression with corresponding attenuation of apoptosis in WT-derived hepatocytes, but not in KO hepatocytes. PMID: 27936029
    4. HNF6 binding was sex independent at a majority of binding sites, and HNF6 peaks were frequently associated with cobinding by multiple other liver transcription factors, consistent with HNF6 playing a global regulatory role in both male and female liver. PMID: 26218442
    5. with Lmx1a to promote ventral midbrain neural stem cells differentiation into dopamine neuron through Wnt1-Lmx1a pathway. PMID: 25747864
    6. Onecut1 and Onecut2 have roles in operating downstream of Pax6 to regulate horizontal cell development PMID: 25794677
    7. similar to Onecut1, Onecut2 is also necessary for the formation of normal numbers of horizontal cells in the developing retina. PMID: 25313862
    8. identified downstream genes of Oc1 and Oc2 that shows that the onecut factors suppress the production of the late cell type, rods, indicating that the two factors contribute to the competence of retinal progenitor cells for early retinal cell fates PMID: 25228773
    9. Sex-dependent growth hormone secretion patterns affect the expression of HNF6 in liver. PMID: 25451687
    10. Liver cell plasticity is competent for regeneration of intrahepatic bile duct independent of Notch signaling via Rbpj and Hnf6. PMID: 24631193
    11. in Hnf6-/- mice, the organization of Purkinje cells became abnormal during a second phase of their development. PMID: 23669529
    12. Insight on the role of HNF6 in pancreatic cancer development could lead to its use as a biomarker for early detection and prognosis. PMID: 24638272
    13. Let-7b, miR-495, and their targets constitute a gene network that is required to establish and maintain pancreatic acinar cell differentiation. Let-7b and miR-495 repress HNF6 and are expressed in developing acini. PMID: 23684747
    14. this study showed that Onecut1 is essential for horizontal cell genesis and retinal integrity in mice. PMID: 23926259
    15. Onecut1 suppresses rod photoreceptor genesis. PMID: 23867227
    16. HNF-6 controls in spinal motor neurons a genetic program that coordinates the formation of hindlimb neuromuscular junctions PMID: 23227180
    17. HNF6 and Sox9 are new biomarkers of pancreatic acinar-to-ductal metaplasia and constitute candidate targets for preventive treatment in cases when acinar-to-ductal metaplasia may lead to cancer. PMID: 22271799
    18. Onecut1 and Onecut2 are expressed in both developing and mature horizontal cells of the retina. PMID: 21830221
    19. HNF-6 and Notch signaling interact in vivo to control expression of downstream mediators essential to the normal development of the intrahepatic biliary system. PMID: 21898486
    20. HNF6 has both early and late functions in the developing pancreas and is essential for maintenance of Ngn3 expression and proper pancreatic duct morphology. PMID: 19766716
    21. These results indicate that the expression of HNF-6 and OC-2 regulators of liver and pancreas development is modulated by miR-495 and miR-218. PMID: 19913497
    22. Our data unveil a complex and dynamic expression profile of the OC proteins in spinal MN, which suggests that these factors may participate in regulatory networks that control different steps of motor neuron development. PMID: 19800948
    23. HNF6 is required for gallbladder formation, for normal differentiation of the BEC(biliary epithelial cells) and for proper morphogenesis of the IHBD(intrahepatic bile ducts) and EHBD(extrahepatic bile ducts). PMID: 11934848
    24. diminished hepatic HNF-6 levels are required for repair in response to biliary injury and that it regulates expression of genes that possess differentiation-specific function that are inhibitory to proliferation. PMID: 12029624
    25. results show that although HNF-6 is not required to open chromatin of the hepatic promoter of the glucokinase gene, it stimulates transcription of the glucokinase gene in the liver PMID: 12189444
    26. the development of pancreatic beta cells, the differentiation of insulin-producing cells in embryoid bodies did not require HNF-6 PMID: 12687336
    27. Because parenchymal cells, but not hemopoietic cells, express hnf6 in normal liver, the s conclude that the transcription factor HNF-6 controls B lymphopoiesis in fetal liver and exerts this control indirectly by acting in parenchymal cells. PMID: 12874218
    28. The role of HNF6 in different stages of pancreatic endocrine cell development is reviewed.The precise temporal requirement of HNF6 in the production of terminally differentiated endocrine cells remains unclear. PMID: 15037986
    29. study determines solution structures of the 162 amino acid residue DNA-binding domain of the hepatic nuclear factor-6alpha protein PMID: 15169783
    30. hepatocyte nuclear factor-6/Onecut-1 controls the expression of its paralog Onecut-3 in developing mouse endoderm PMID: 15381696
    31. Onecut transcription factor HNF-6 inhibits activin/TGF beta signaling in the parenchyma, and this allows normal hepatocyte differentiation. PMID: 16103213
    32. The sequential activation of vHNF1, HNF6, and Pdx1 in the endoderm appears to control the generation of pancreatic precursors. PMID: 16380477
    33. HNF-6 controls a network of genes involved in cilium formation and in hereditary polycystic diseases. PMID: 16472605
    34. In liver regeneration hepatocyte nuclear factor 6(HNF6)levels stimulate hepatocyte proliferation through transcriptional induction of cell cycle regulatory genes. PMID: 16618419
    35. Downregulation of Hnf6 expression in beta-cells during development is essential to achieve a mature, glucose-responsive beta-cell. PMID: 17130469
    36. HNF-6 and OC-2 play partially redundant roles in developing pancreas, antero-posterior patterning of the gut and in enteroendocrine differentiation. PMID: 17400205
    37. Data suggest that the Onecut transcription factors HNF-6/OC-1 and OC-2 regulate early liver expansion by controlling hepatoblast migration. PMID: 17936262
    38. the GH/HNF-6 axis is a potential in vivo mechanism underlying GH diverse function in the liver to modulate the liver repair response to bile duct ligation. PMID: 18511741

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  • 亚细胞定位：
    Nucleus.
  • 蛋白家族：
    CUT homeobox family
  • 数据库链接：

    KEGG: mmu:15379

    STRING: 10090.ENSMUSP00000058020

    UniGene: Mm.303355