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  4. Recombinant Mouse Growth factor receptor-bound protein 10 (Grb10)

Recombinant Mouse Growth factor receptor-bound protein 10 (Grb10)

  • 货号：
    CSB-YP009887MO
  • 规格：
  • 来源：
    Yeast
  • 其他：
  • 货号：
    CSB-EP009887MO
  • 规格：
  • 来源：
    E.coli
  • 其他：
  • 货号：
    CSB-EP009887MO-B
  • 规格：
  • 来源：
    E.coli
  • 共轭：
    Avi-tag Biotinylated

    E. coli biotin ligase (BirA) is highly specific in covalently attaching biotin to the 15 amino acid AviTag peptide. This recombinant protein was biotinylated in vivo by AviTag-BirA technology, which method is BriA catalyzes amide linkage between the biotin and the specific lysine of the AviTag.

  • 其他：
  • 货号：
    CSB-BP009887MO
  • 规格：
  • 来源：
    Baculovirus
  • 其他：
  • 货号：
    CSB-MP009887MO
  • 规格：
  • 来源：
    Mammalian cell
  • 其他：

产品详情

  • 纯度：
    >85% (SDS-PAGE)
  • 基因名：
  • Uniprot No.：
  • 别名：
    Grb10; Meg1Growth factor receptor-bound protein 10; GRB10 adapter protein; Maternally expressed gene 1 protein
  • 种属：
    Mus musculus (Mouse)
  • 蛋白长度：
    full length protein
  • 表达区域：
    1-621
  • 氨基酸序列
    MNNDINSSVE SLNSACNMQS DTDTAPLLED GQHASNQGAA SSSRGQPQAS PRQKMQRSQP VHILRRLQEE DQQLRTASLP AIPNPFPELT GAAPGSPPSV APSSLPPPPS QPPAKHCGRC EKWIPGENTR GNGKRKIWRW QFPPGFQLSK LTRPGLWTKT TARFSKKQPK NQCPTDTVNP VARMPTSQME KLRLRKDVKV FSEDGTSKVV EILTDMTARD LCQLLVYKSH CVDDNSWTLV EHHPQLGLER CLEDHEIVVQ VESTMPSESK FLFRKNYAKY EFFKNPVNFF PDQMVNWCQQ SNGGQAQLLQ NFLNTSSCPE IQGFLQVKEV GRKSWKKLYV CLRRSGLYYS TKGTSKEPRH LQLLADLEES SIFYLIAGKK QYNAPNEHGM CIKPNKAKTE MKELRLLCAE DEQIRTCWMT AFRLLKYGML LYQNYRIPQR KGLPPPFNAP MRSVSENSLV AMDFSGQIGR VIDNPAEAQS AALEEGHAWR KRSTRMNILS SQSPLHPSTL NAVIHRTQHW FHGRISREES HRIIKQQGLV DGLFLLRDSQ SNPKAFVLTL CHHQKIKNFQ ILPCEDDGQT FFTLDDGNTK FSDLIQLVDF YQLNKGVLPC KLKHHCIRVA L
  • 蛋白标签：
    Tag type will be determined during the manufacturing process.
    The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.
  • 产品提供形式：
    Lyophilized powder
    Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
  • 复溶：
    We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
  • 储存条件：
    Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
  • 保质期：
    The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
    Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
  • 货期：
    Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
    Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
  • 注意事项：
    Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
  • Datasheet ：
    Please contact us to get it.

产品评价

靶点详情

  • 功能：
    Adapter protein which modulates coupling of a number of cell surface receptor kinases with specific signaling pathways. Binds to, and suppress signals from, activated receptors tyrosine kinases, including the insulin (INSR) and insulin-like growth factor (IGF1R) receptors. The inhibitory effect can be achieved by 2 mechanisms: interference with the signaling pathway and increased receptor degradation. Delays and reduces AKT1 phosphorylation in response to insulin stimulation. Blocks association between INSR and IRS1 and IRS2 and prevents insulin-stimulated IRS1 and IRS2 tyrosine phosphorylation. Recruits NEDD4 to IGF1R, leading to IGF1R ubiquitination, increased internalization and degradation by both the proteasomal and lysosomal pathways. A similar role in the mediation of ubiquitination has also been suggested with INSR. Negatively regulates Wnt signaling by interacting with LRP6 intracellular portion and interfering with the binding of AXIN1 to LRP6. Positive regulator of the KDR/VEGFR-2 signaling pathway. May inhibit NEDD4-mediated degradation of KDR/VEGFR-2.
  • 基因功能参考文献：
    1. ablation of growth factor receptor bound protein 10 (Grb10) in muscle is sufficient to affect muscle size and metabolism, supporting an important role for this protein in growth and metabolic pathways PMID: 29370381
    2. DNA methylation at CpG island 1 (CGI1) of Grb10 in blastocysts were also significantly decreased after vitrification PMID: 28696244
    3. This study reveals a function for the imprinted gene Grb10 in regulating hematopoietic stem cell self-renewal. PMID: 27806297
    4. In tumors, Grb10 loss independently promotes Ras pathway hyperactivation, which promotes hyperproliferation, an early feature of tumor development. PMID: 26000738
    5. This study identified Grb10 as a critical regulator of lipid metabolism, the programming of the browning phenotype, and thermogenesis in adipose tissues. PMID: 24746805
    6. Negative regulation of Grb10 Interacting GIGYF2 protein on IGF-1 receptor signaling pathway caused diabetic mice cognitive impairment. PMID: 25268761
    7. Grb10 is involved in BCR-ABL-positive leukemia in mice. PMID: 25249015
    8. Grb10 is a key genetic component of developmental programming. PMID: 24586114
    9. Glucose uptake was higher in Grb10(-/-) primary myotubes in the basal state and was associated with enhanced insulin signaling and an increase in GLUT4 translocation to the plasma membrane. PMID: 24664951
    10. Grb10 expression in mouse embryo is regulated by Lmx1a transcription factor. PMID: 23106268
    11. Results suggest consecutive passaging may affect epigenetic modifications of Grb10 in adult fibroblast cells. PMID: 22971350
    12. Study has identified Grb10 as an important regulator of beta-cell proliferation and demonstrated that reducing the expression level of Grb10 could provide a novel means to increase beta-cell mass and reduce beta-cell apoptosis. PMID: 22923474
    13. Grb10-ablated muscle from adult mice shows coordinate gene changes that oppose those of muscle wasting pathologies, highlighting Grb10 as a potential therapeutic target for these conditions PMID: 22623587
    14. Data suggest that GRB10 is involved in pancreatic alpha cell survival and, as result, plays an important role in regulating basal glucagon secretion and glucose tolerance; studies include both apoptotic and anti-apoptotic signaling of GRB10. PMID: 22222503
    15. mTORC1-mediated phosphorylation stabilized Grb10, leading to feedback inhibition of the phosphatidylinositol 3-kinase (PI3K) and extracellular signal-regulated, mitogen-activated protein kinase (ERK-MAPK) pathways PMID: 21659605
    16. analysis of the interaction between the growth factor-binding protein GRB10 and the E3 ubiquitin ligase NEDD4 PMID: 20980250
    17. Grb10 is, so far, a unique imprinted gene, able to influence distinct physiological processes, fetal growth and adult behaviour, owing to actions of the two parental alleles in different tissues PMID: 21270893
    18. Grb10 gene is expressed from the paternal allele in brain, but from the maternal allele in most other tissues. PMID: 17101788
    19. These results suggest a role for the Grb10/Nedd4 complex in regulating ubiquitination and stability of the IGF-IR, and they suggest that Grb10 serves as an adapter to form a bridge between Nedd4 and the IGF-IR. PMID: 12697834
    20. Grb10 is a critical component of the insulin receptor (IR) signaling complex that provides a functional link between IR and p85 phosphatidylinositol (PI) 3-kinase and regulates PI 3-kinase activity. PMID: 12783867
    21. The majority of Grb10 expression arising from the maternally inherited allele. Grb10 action is essentially independent of insulin-like growth factor 2. Grb10 is a potent growth inhibitor. PMID: 12829789
    22. Ovexpressed Grb10 effects insulin-regulated glucose transport. PMID: 15664450
    23. Taken together, these results indicate that Meg1/Grb10 interacts with both insulin and IGF1 receptors in vivo, and negatively regulates the IGF growth pathways via these receptors. PMID: 15752742
    24. Grb10 functions as a specific endogenous suppressor of IGF-I-stimulated cell signaling and DNA synthesis. PMID: 16037382
    25. Grb10 adapter protein creates a subpopulation of Raf-1 with specific anti-apoptotic activity in fibroblasts. PMID: 17535812
    26. Grb10 forms a link between fetal growth and glucose-regulated metabolism in postnatal life and is a candidate for involvement in the process of fetal programming of adult metabolic health. PMID: 17562854
    27. Peripheral disruption of the Grb10 gene enhances insulin signaling and sensitivity in vivo. PMID: 17620412
    28. These results indicate that Grb10 and Nedd4 play a critical role in mediating IGF-IR down-regulation by promoting ligand-dependent multiubiquitination of the IGF-IR, which is required for receptor internalization and regulates mitogenesis. PMID: 18286479
    29. With high fat diet, the diabetes onset rate of Meg1 Tg mice increased up to 60%. These results suggest that Meg1 Tg mice resemble human 2DM. PMID: 18633161
    30. Data indicate that bivalent chromatin, in combination with neuronal factors, controls the paternal expression of Grb10 in brain. PMID: 18650936
    31. study reports co-localization of adaptor proteins nShc & Grb10, with Kv1.3 channel in the olfactory bulb neural lamina; BDNF-induced current suppression of the Kv1.3 channel was prevented by both nShc and Grb10 PMID: 19166614
    32. Fetal and neonatal growth was very sensitive to the expression level of Meg1/Grb10 Type I, indicating that the 2-fold increment of the Meg1/Grb10 Type I is one of the major causes of the growth retardation. PMID: 19174477
    33. Grb10 and Grb14 have roles in regulation of insulin signaling and glucose homeostasis PMID: 19541746
    34. Grb10 may play an important role during the development process in mouse. PMID: 19586879
    35. Grb10 can influence reproductive strategy through the allocation of maternal resources such that offspring number is offset against size. PMID: 19833122

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  • 亚细胞定位：
    Cytoplasm. Note=When complexed with NEDD4 and IGF1R, follows IGF1R internalization, remaining associated with early endosomes. Uncouples from IGF1R before the sorting of the receptor to the lysosomal compartment.
  • 蛋白家族：
    GRB7/10/14 family
  • 组织特异性：
    Widely expressed.
  • 数据库链接：

    KEGG: mmu:14783

    STRING: 10090.ENSMUSP00000091011

    UniGene: Mm.273117