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  4. Recombinant Mouse G1/S-specific cyclin-E1 (Ccne1)

Recombinant Mouse G1/S-specific cyclin-E1 (Ccne1)

  • 货号：
    CSB-YP723411MO
  • 规格：
  • 来源：
    Yeast
  • 其他：
  • 货号：
    CSB-EP723411MO
  • 规格：
  • 来源：
    E.coli
  • 其他：
  • 货号：
    CSB-EP723411MO-B
  • 规格：
  • 来源：
    E.coli
  • 共轭：
    Avi-tag Biotinylated

    E. coli biotin ligase (BirA) is highly specific in covalently attaching biotin to the 15 amino acid AviTag peptide. This recombinant protein was biotinylated in vivo by AviTag-BirA technology, which method is BriA catalyzes amide linkage between the biotin and the specific lysine of the AviTag.

  • 其他：
  • 货号：
    CSB-BP723411MO
  • 规格：
  • 来源：
    Baculovirus
  • 其他：
  • 货号：
    CSB-MP723411MO
  • 规格：
  • 来源：
    Mammalian cell
  • 其他：

产品详情

  • 纯度：
    >85% (SDS-PAGE)
  • 基因名：
  • Uniprot No.：
  • 别名：
    Ccne1; Ccne; G1/S-specific cyclin-E1
  • 种属：
    Mus musculus (Mouse)
  • 蛋白长度：
    full length protein
  • 表达区域：
    1-408
  • 氨基酸序列
    MPRERDSTDH SNMKEEGGSD LSVRSRKRKA NVAVFLQDPD EEIAKIDKTV KSEDSSQPWD DNSACVDPCS FIPTPNKEED NELEYPRTAF QPRKIRPPRA SPLPVLNWGN REEVWRIMLN KEKTYLRDEH FLQRHPLLQA RMRAVLLDWL MEVCEVYKLH RETFYLAQDF FDRYMASQHN IIKTLLQLIG ISALFIASKL EEIYPPKLHQ FAYVTDGACS GDEILTMELM MMKALKWRLS PLTIVSWLNV YVQVAYVNDT GEVLMPQYPQ QVFVQIAELL DLCVLDVGCL EFPYGVLAAS ALYHFSSLEL MQKVSGYQWC DIEKCVKWMV PFAMVIREMG SSKLKHFRGV PMEDSHNIQT HTNSLDLLDK AQAKKAILSE QNRISPPPSV VLTPPPSSKK QSSEQETE
  • 蛋白标签：
    Tag type will be determined during the manufacturing process.
    The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.
  • 产品提供形式：
    Lyophilized powder
    Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
  • 复溶：
    We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
  • 储存条件：
    Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
  • 保质期：
    The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
    Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
  • 货期：
    Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
    Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
  • 注意事项：
    Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
  • Datasheet ：
    Please contact us to get it.

产品评价

靶点详情

  • 功能：
    Essential for the control of the cell cycle at the G1/S (start) transition.
  • 基因功能参考文献：
    1. study suggests that hepatocellular carcinoma initiation specifically depends on CcnE1 and Cdk2, while HCC progression requires expression of any E-cyclin, but no Cdk2. PMID: 30150405
    2. ChIP-sequence/ChIP studies indicated that Ad4BP/SF-1 binds to the upstream region of Ccne1 (cyclin E1) gene during G1/S phase. PMID: 28325912
    3. These results demonstrate a repressor role for NFAT1 in cell cycle progression and Cyclin E expression in B lymphocytes, and suggest a potential function for NFAT1 protein in B cell malignancies. PMID: 27399331
    4. This approach allowed us to determine the identity of cyclin E protein partners, as well as phosphorylation substrates of cyclins E (cyclin E1and cyclin E2)and its associated kinase, Cdk2, in different mouse organs. PMID: 27828963
    5. Spermatocytes lacking cyclin E2 and one E1 allele (E1+/-E2-/-) displayed a high rate of telomere abnormalities but can progress to pachytene and diplotene stages. PMID: 26712234
    6. Myb regulates Cyclin E1 expression in normal gastrointestinal tract epithelial cells and is required during intestinal tumorigenesis PMID: 25934694
    7. These results highlight a new role for E-type cyclins (Ccne1 and Ccne2) as important regulators of male meiosis. PMID: 24586195
    8. Concurrent deletion of cyclin E1 and cyclin-dependent kinase 2 in hepatocytes inhibits DNA replication and liver regeneration in mice. PMID: 23787781
    9. Superoxide dismutase induces G1-phase cell cycle arrest by down-regulated expression of Cdk-2 and cyclin-E in sarcoma tumor cells. PMID: 23055394
    10. Ablation of cyclin E led to a decreased number of synapses, reduced number and volume of dendritic spines, and resulted in impaired synaptic plasticity and memory formation. PMID: 21944720
    11. Data show that gastric cancer markers MUC2, and oncogenes c-myc, cyclin E1 were expressed in the gastric carcinoma cell line 3I (MGCC3I). PMID: 20737421
    12. our fi ndings reveal a direct link between cyclin E and HIF-1 activities in mammary epithelial cells and implicate HIF-1 as a mediator of proliferation-independent phenotypes associated with high cyclin E expression in some human breast cancers. PMID: 21746877
    13. Report presents the first detailed analysis of cyclin E expression in postmitotic neurons during development and in the adult mouse brain PMID: 20863901
    14. Results show that deregulation of cyclin E expression contribute to infertility, due to inability of the spermatogonial cells to start the mitotic cycles prior to entering meiosis. PMID: 20962587
    15. Depletion of cyclin E promoted apoptosis of breast cancer cells and blocked proliferation and transformation. PMID: 20877462
    16. These results suggest that cyclin E up-regulation and p27(Kip1) loss-of-function act cooperatively on pituitary adenoma development. PMID: 20660298
    17. Data show that liver-specific ablation of the cyclin E regulator Cullin 3 in mice results in the persistence and massive expansion of hepatic progenitor cells. PMID: 20978349
    18. cyclin E has a role in megakaryocytic polyploidization PMID: 20392692
    19. Cyclin E is a target of WT1 transcriptional repression PMID: 11919196
    20. Involvement of CDK2/cyclin E and nucleophosmin/B23 in centrosome duplication PMID: 12429912
    21. Data suggest that the interaction between PKCeta and cyclin E is carefully regulated, and is correlated with the inactivated form of the cyclin E/Cdk2 complex. PMID: 12729791
    22. These findings define a molecular function for E type cyclins (cyclins E1 and E2) in cell cycle reentry and reveal a differential requirement for cyclin E in normal versus oncogenic proliferation. PMID: 12941272
    23. During embryonic development, the needs for this cyclin can be overcome in mitotic cycles but not in endoreplicating cells. PMID: 12970191
    24. essential functions for Fbw7 in controlling cyclin E and Notch signaling pathways PMID: 14766969
    25. This work indicates that-in addition to their function as CDK activators-E cyclins play kinase-independent functions in cell-cycle progression. PMID: 17218276
    26. cyclin E has a role in dysplasia and multiple pulmonary adenocarcinomas PMID: 17360482
    27. Akt-mediated liver growth promotes induction of cyclin E through a novel translational mechanism and a p21-mediated cell cycle arrest PMID: 17517888
    28. Overexpression of the low molecular weight cyclin E in transgenic mice induces metastatic mammary carcinomas through the disruption of the ARF-p53 pathway PMID: 17671189
    29. Cyclin E phosphorylation regulates cell proliferation in hematopoietic and epithelial lineages in vivo. PMID: 18559482
    30. a mechanism for the G(1)/S regulation with increased levels of p27(Kip1) binding to Cdk1/cyclin E complexes was elicited in Cdk2(-/-)Cdk4(-/-)Rb(-/-) (TKO) mutant mice embryonic fibroblasts PMID: 19129496
    31. Data indicate that the CDK2/cyclin E/p220(NPAT)/HINFP/histone gene signaling pathway at the G1/S phase transition is an essential, nonredundant cell cycle regulatory mechanism that is established early in embryogenesis. PMID: 19590016
    32. By interacting with Ku70, a cyclin E fragment liberates Bax, which participates in apoptosis by sustaining a positive feedback loop targeting mitochondria. This process is independent of p53 and new RNA or protein synthesis. PMID: 17581275

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  • 亚细胞定位：
    Nucleus.
  • 蛋白家族：
    Cyclin family, Cyclin E subfamily
  • 组织特异性：
    Found in adult spleen, and to a lesser extent in adult testis and brain.
  • 数据库链接：

    KEGG: mmu:12447

    STRING: 10090.ENSMUSP00000103658

    UniGene: Mm.16110