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  4. Recombinant Mouse Cytochrome b-245 light chain (Cyba)

Recombinant Mouse Cytochrome b-245 light chain (Cyba)

  • 货号：
    CSB-YP723412MO
  • 规格：
  • 来源：
    Yeast
  • 其他：
  • 货号：
    CSB-EP723412MO
  • 规格：
  • 来源：
    E.coli
  • 其他：
  • 货号：
    CSB-EP723412MO-B
  • 规格：
  • 来源：
    E.coli
  • 共轭：
    Avi-tag Biotinylated

    E. coli biotin ligase (BirA) is highly specific in covalently attaching biotin to the 15 amino acid AviTag peptide. This recombinant protein was biotinylated in vivo by AviTag-BirA technology, which method is BriA catalyzes amide linkage between the biotin and the specific lysine of the AviTag.

  • 其他：
  • 货号：
    CSB-BP723412MO
  • 规格：
  • 来源：
    Baculovirus
  • 其他：
  • 货号：
    CSB-MP723412MO
  • 规格：
  • 来源：
    Mammalian cell
  • 其他：

产品详情

  • 纯度：
    >85% (SDS-PAGE)
  • 基因名：
  • Uniprot No.：
  • 别名：
    CybaCytochrome b-245 light chain; Cytochrome b(558) alpha chain; Cytochrome b558 subunit alpha; Neutrophil cytochrome b 22 kDa polypeptide; Superoxide-generating NADPH oxidase light chain subunit; p22 phagocyte B-cytochrome; p22-phox; p22phox
  • 种属：
    Mus musculus (Mouse)
  • 蛋白长度：
    Full Length of Mature Protein
  • 表达区域：
    2-192
  • 氨基酸序列
    GQIEWAMWA NEQALASGLI LITGGIVATA GRFTQWYFGA YSIAAGVLIC LLEYPRGKRK KGSTMERCGQ KYLTSVVKLF GPLTRNYYVR AALHFLLSVP AGFLLATILG TVCLAIASVI YLLAAIRGEQ WTPIEPKPKE RPQVGGTIKQ PPTNPPPRPP AEVRKKPSEG EEEAASAGGP QVNPMPVTDE VV
  • 蛋白标签：
    Tag type will be determined during the manufacturing process.
    The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.
  • 产品提供形式：
    Lyophilized powder
    Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
  • 复溶：
    We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
  • 储存条件：
    Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
  • 保质期：
    The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
    Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
  • 货期：
    Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
    Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
  • 注意事项：
    Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
  • Datasheet ：
    Please contact us to get it.

产品评价

靶点详情

  • 功能：
    Critical component of the membrane-bound oxidase of phagocytes that generates superoxide. Associates with NOX3 to form a functional NADPH oxidase constitutively generating superoxide.
  • 基因功能参考文献：
    1. In p22phox(-/-) mice, hypoxic pulmonary vasoconstriction (HPV) was significantly impaired. In the chronic hypoxic setting, lack of p22phox was associated with improved right ventricular function and decreased pulmonary vascular remodelling. PMID: 28729471
    2. Together with the increased p22phox expression in lungs of asthmatic patients, findings demonstrate a crucial role of p22phox-dependent NADPH oxidase for the development of mucus hypersecretion and airway hyperresponsiveness in house dust mite-induced model of asthma. PMID: 28510479
    3. Identification of a PPAR-gamma --> NF-kappaB --> p22phox neuroprotective signaling cascade opens a new avenue for protecting the brain against ischemic insult. PMID: 26108185
    4. Knockout of the cytochrome P450 reductase by CRISPR/Cas9 technology (POR(-/-)) in HEK293 cells overexpressing Nox4 or Nox5 did not interfere with ROS production in intact cells. However, POR(-/-) abolished the signal in NADPH-stimulated assays using membrane fractions from the very same cells. Moreover, membranes of rat smooth muscle cells treated with angiotensin II showed an increased NADPH-dependent signal with lucigen PMID: 27863990
    5. p22phox mRNA expression was increased in diet-induced obese (DIO) mice. PMID: 26297224
    6. The present study provides evidence that augmented EGFRtk impairs vascular function by NADPH oxidase-dependent mechanism. Therefore, EGFRtk and oxidative stress should be potential targets to treat vascular dysfunction in TD2. PMID: 26036345
    7. Vascular hnRNP-C expression is regulated by ROS derived from NADPH oxidases and that the effects of NADPH oxidase on vascular activation are mediated in part by protein kinase CK1alpha. PMID: 24035466
    8. Enhanced p22(phox) expression causes vascular dysfunction through ERK1/2 and p38-mitogen-activated protein kinase-dependent mechanisms in male type 2 diabetic mice. PMID: 24486509
    9. The first quantitative characterization of the interactions made between the cytosolic regulators NOXO1 and NOXA1 and membrane-bound p22(phox), is presented. PMID: 20454568
    10. renal expression of Nox-2, p22(phox), and p47(phox), components of NADPH oxidase, are upregulated in GSD-Ia mice compared with controls PMID: 20195241
    11. cytochrome b558 expression is downregulated via the reduction of heme availability after NADPH oxidase is inhibited by HO-1 PMID: 15123630
    12. role in resistance to mucosal and systemic candidiasis of endogenous origin PMID: 15731028
    13. LPS treatment enhanced protein levels of p22(phox), a catalytic subunit of NADPH oxidase, and increased NADPH oxidase activity and levels of superoxide radicals and hydrogen peroxide. PMID: 16973969
    14. p22phox expression was increased during glucose-induced oxidative stress in microvascular endothelial cells. PMID: 17443690
    15. collagen synthesis in cardiac fibroblasts involves a facilitative interaction between TGFbeta(1)-NADPH oxidase and LOX-1 PMID: 18182394
    16. mice of the nmf333 strain, animal model of p22(phox) deficiency, exhibit a compound phenotype consisting of both a CGD-like immune defect and a balance disorder caused by the aberrant development of gravity-sensing organs. PMID: 18292807
    17. Suggest that a NOX4 isotype plus p22 phox account for the swelling-induced increase in the reactive oxygen species production in NIH3T3 cells. PMID: 18417717
    18. p22phox is expressed in the retinal pigment epithelial cells and inner retinal neurons. A small-interfering RNA designed against p22phox efficiently reduced the expression of the protein in the eye when delivered by recombinant adeno-associated virus PMID: 18665154
    19. Chronic intermittent hypoxia-induced pulmonary hypertension was associated with increased lung levels of the NADPH oxidase subunits, Nox4 and p22phox. PMID: 18952568
    20. p22phox is involved in invadopodia formation; Tks5 and p22(phox) can associate with each other, suggesting that Tks5 is part of the Nox complex PMID: 19755709

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  • 亚细胞定位：
    Cell membrane.
  • 蛋白家族：
    P22phox family
  • 组织特异性：
    The strongest level of expression is found in kidney, peritoneal neutrophils and peritoneal macrophages, and a lower level in spleen and small intestine. Very low level of expression can be noted in brain, liver, testis, and heart.
  • 数据库链接：

    KEGG: mmu:13057

    STRING: 10090.ENSMUSP00000017604

    UniGene: Mm.271671