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  4. Recombinant Mouse Cyclin-dependent kinase inhibitor 1C (Cdkn1c)

Recombinant Mouse Cyclin-dependent kinase inhibitor 1C (Cdkn1c)

  • 货号：
    CSB-YP005088MO
  • 规格：
  • 来源：
    Yeast
  • 其他：
  • 货号：
    CSB-EP005088MO
  • 规格：
  • 来源：
    E.coli
  • 其他：
  • 货号：
    CSB-EP005088MO-B
  • 规格：
  • 来源：
    E.coli
  • 共轭：
    Avi-tag Biotinylated

    E. coli biotin ligase (BirA) is highly specific in covalently attaching biotin to the 15 amino acid AviTag peptide. This recombinant protein was biotinylated in vivo by AviTag-BirA technology, which method is BriA catalyzes amide linkage between the biotin and the specific lysine of the AviTag.

  • 其他：
  • 货号：
    CSB-BP005088MO
  • 规格：
  • 来源：
    Baculovirus
  • 其他：
  • 货号：
    CSB-MP005088MO
  • 规格：
  • 来源：
    Mammalian cell
  • 其他：

产品详情

  • 纯度：
    >85% (SDS-PAGE)
  • 基因名：
  • Uniprot No.：
  • 别名：
    Cdkn1c; Kip2; Cyclin-dependent kinase inhibitor 1C; Cyclin-dependent kinase inhibitor p57; p57Kip2
  • 种属：
    Mus musculus (Mouse)
  • 蛋白长度：
    Full length protein
  • 表达区域：
    1-348
  • 氨基酸序列
    MGMSDVYLRS RTAMERLASS DTFPVIARSS ACRSLFGPVD HEELGRELRM RLAELNAEDQ NRWDFNFQQD VPLRGPGRLQ WMEVDSESVP AFYRETVQVG RCRLQLGPRP PPVAVAVIPR SGPPAGEAPD GLEEAPEQPP SAPASAVVAE PTPPATPAPA SDLTSDPIPE VTLVATSDPT PDPIPDANPD VATRDGEEQV PEQVSEQGEE SGAEPGDELG TEPVSEQGEE QGAEPVEEKD EEPEEEQGAE PVEEQGAEPV EEQNGEPVEE QDENQEQRGQ ELKDQPLSGI PGRPAPGTAA ANANDFFAKR KRTAQENKAS NDVPPGCPSP NVAPGVGAVE QTPRKRLR
  • 蛋白标签：
    Tag type will be determined during the manufacturing process.
    The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.
  • 产品提供形式：
    Lyophilized powder
    Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
  • 复溶：
    We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
  • 储存条件：
    Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
  • 保质期：
    The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
    Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
  • 货期：
    Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
    Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
  • 注意事项：
    Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
  • Datasheet ：
    Please contact us to get it.

产品评价

靶点详情

  • 功能：
    Potent tight-binding inhibitor of several G1 cyclin/CDK complexes (cyclin E-CDK2, cyclin D2-CDK4, and cyclin A-CDK2) and, to lesser extent, of the mitotic cyclin B-CDC2. Negative regulator of cell proliferation. May play a role in maintenance of the non-proliferative state throughout life.
  • 基因功能参考文献：
    1. Cdkn1c expression and methylation may associate with cell cycle exit and differentiation of odontoblasts. PMID: 30014245
    2. progenitors are dependent on p57(KIP2)-mediated slowing of replication forks for self-renewal, a novel function for cyclin-dependent kinase inhibitors. The switch to differentiation entails rapid down-regulation of p57(KIP2) with a consequent global increase in replication fork speed and an abruptly shorter S phase. PMID: 28560351
    3. Our data indicate that mouse embryonic stem cells are induced into islet-like cells in vitro. The gene imprinting status of Kcnq1 and Cdkn1c may be changed in differentiated cells during the induction in vitro. PMID: 28926866
    4. the consequence of elevated Cdkn1c expression on dopamine-related behaviours highlighting the importance of correct dosage of this imprinted gene in the brain PMID: 28857482
    5. Immunohistochemistry revealed robust p57(kip2) staining in trophoblast giant cells and in the ectoplacental cone at E8.5. p57(kip2) protein was seen in giant cells and throughout the labyrinth, although its abundance was reduced in the junctional zone at E9.5, and became more diffuse by E12.5. PMID: 26988311
    6. Cdkn1c-luciferase mice offer non-invasive tools to identify factors that disrupt epigenetic processes. PMID: 28147266
    7. this study identifies E2A target genes in embryonic neural stem cells and demonstrates that E47 regulates neuronal differentiation via p57(KIP2). PMID: 28939666
    8. This is the first report linking elevated Cdkn1c to altered behaviour in mice. Importantly, the findings from our study may have relevance for Silver Russell Syndrome and highlight a potentially underreported aspect of this disorder. PMID: 27798108
    9. Dnmt3a-cKO muscles exhibit fewer Pax7+ SCs, which show increased expression of p57Kip2 protein PMID: 27415617
    10. indicate that the effects of insulin-like growth factor 2 (IGF2) are mediated by direct upregulation of the cyclin-dependent kinase inhibitor p57 (p57). PMID: 26872540
    11. Data show that transforming growth factor beta (TGFbeta)-induced changes in Gata2 transcription factor and cyclin-dependent kinase inhibitor 1C (P57) expression in hematopoietic progenitors are conveyed through Smad signaling via Smad4 protein. PMID: 26876150
    12. This study reveals a key requirement for Cdkn1c in the early development of the brown adipose lineages. PMID: 26963625
    13. Lhx6 and Lhx8 promote palate development through negative regulation of a cell cycle inhibitor gene, p57Kip2 PMID: 26071365
    14. hepatoblasts in p57(Kip2)-/- mice were highly proliferative and had deficient maturation compared with those in wild-type (WT) mice. PMID: 26165599
    15. Data indicate that cyclin-dependent kinase inhibitor 1C (P57 was post-transcriptionally regulated by microRNA miR-221 in embryonic stem (ES) cells. PMID: 25086570
    16. Data show that disruption of potassium voltage-gated channel, KQT-like subfamily Q, member1 (KCNQ1) results in increased expression of cyclin-dependent kinase inhibitor 1C (Cdkn1c) only when the mutation is on the paternal allele. PMID: 26100882
    17. In the absence of maintained DNA methylation (Dnmt1(-/-)), Cdkn1c escapes silencing demonstrating the requirement for DNA methylation in long term silencing in vivo. PMID: 20372090
    18. A muscle-specific regulatory element of p57(kip2) directly activated by muscle regulatory factors in myoblasts but repressed by the Notch targets Hes1/Hey1 in progenitor cells, is identified. PMID: 25005473
    19. The regulation of NSC quiescence by p57 might thus have important implications for the short-term (extrinsic stimuli-dependent) and long-term (age-related) modulation of neurogenesis. PMID: 23481253
    20. A novel function was uncovered for p57 that integrates external signals transduced by p38 stress-activated protein kinase to control the cell-cycle, establishing a checkpoint in G1 which is critical to permit cellular adaptation to stress. PMID: 22569127
    21. p57(Kip2) coordinates multiple stages of corticogenesis and exhibits distinct and common activities compared with related family member p27(Kip1). PMID: 21245411
    22. We particularly focus our attention on p57(Kip2) changes in cancers and pharmacological approaches for modulating p57(Kip2) levels. PMID: 21816904
    23. Cdkn1c mutants attained a 20% increase in weight during gestation but experienced a rapid reversal of this positive growth trajectory very late in gestation. PMID: 21729874
    24. The data suggested that p57KIP2 and p27KIP1 control neuronal output for distinct cortical layers by regulating different stages of precursor proliferation. PMID: 22223678
    25. cooperates with p27(Kip1)to maintain hematopoietic stem cell quiescence through interactions with Hsc70 PMID: 21885020
    26. Observations indicate that p57Kip2 regulates the activities of E2f1 and p53 to ensure the proper proliferation and survival of cells in the subcommissural organ (SCO) as well as of Pax2-positive cells in the cerebellar primordium. PMID: 21844226
    27. Cdkn1c, an imprinted gene with expression only from the maternal allele, is the major regulator of embryonic growth in the IC2 imprinted domain on mouse distal chromosome 7. PMID: 17517131
    28. Data provide evidence that sik1 via p57Kip2 plays a central role in the time clock mechanism that regulates cardiac cell proliferation and differentiation. PMID: 20140255
    29. Induction of p57(KIP2) expression by p73beta PMID: 11891335
    30. VEGF has a role in placental hyperplasia of p57Kip2 null mouse embryos PMID: 12482580
    31. SCFSkp2 complex plays an important role in cell-cycle progression by determining the abundance of p57Kip2 and that of the related CDK inhibitor p27Kip1. PMID: 12925736
    32. p57KIP2 modulates stress-activated signaling by functioning as an endogenous inhibitor of JNK/SAPK PMID: 12963725
    33. p57Kip2 influences postmitotic differentiation of dopamine neurons. PMID: 14671317
    34. p57kip2 mediates proliferative actions of PTHrP in chondrocytes. PMID: 15124025
    35. p57(Kip2) is involved in the regulation of several aspects of the T cell cycle PMID: 15294951
    36. Methylation of the 5' DMR plays an important role in the imprinting of the Cdkn1c gene. PMID: 15647320
    37. There is a marked loss or absence of p57(kip2) expression and high expression of PCNA in hepatocellular carcinoma PMID: 15754413
    38. Cdkn1c gene expression was analyzed at three different stages in differentiating MyoD(-)C2C12 myoblasts PMID: 15890200
    39. Maintenance of bone marrow side population cells in G0/G1 may be controlled by gene expression of p57(Kip2). PMID: 16176803
    40. Recent advances in epigenetic control of the CDKN1C/KCNQ1OT1 imprinted domain in both humans and mice, causing Beckwith-Wiedemann syndrome and cancer. PMID: 16575194
    41. Hes1 and p57 coordinate cell cycle exit and self-renewal of pancreatic progenitors during an early stage in organogenesis to regulate the number of pancreatic progenitors.They also eliminate cells with aberrant cell cycle characteristics. PMID: 16899237
    42. Data show that the Notch effector Herp2 is expressed in lens epithelium and directly suppresses p57Kip2 expression, providing a molecular link between Notch signaling and the cell cycle control machinery during lens development. PMID: 17709399
    43. These data suggest that forced cardiac expression of p57Kip2 does not affect myocardial growth, differentiation and baseline function but attenuates injury from ischemia-reperfusion in the adult mouse heart. PMID: 18312674
    44. cyclin-dependent kinase inhibitor p57(Kip2) and vascular endothelial growth factor mRNAs are selectively translated by an IRES-independent mechanism under hypoxic stress PMID: 18430730
    45. p57(Kip2) is an important gene in prostate cancer tumorigenesis PMID: 18483241
    46. Data show that the minimal promoter element able to confer MyoD responsiveness contains multiple Sp1 and Egr1 recognition sites and that both transcription factors are necessary for the increase in p57 RNA. PMID: 18513743
    47. C/EBPbeta transactivates p57(Kip2) to promote transition from proliferation to hypertrophic differentiation of chondrocytes during endochondral ossification PMID: 19229324

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  • 亚细胞定位：
    Nucleus.
  • 蛋白家族：
    CDI family
  • 组织特异性：
    Expressed in the heart, brain, lung, skeletal muscle, kidney, pancreas and testis. High levels are seen in the placenta while low levels are seen in the liver.
  • 数据库链接：

    KEGG: mmu:12577

    STRING: 10090.ENSMUSP00000128828

    UniGene: Mm.168789