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  4. Recombinant Mouse Cell cycle checkpoint control protein RAD9A (Rad9a)

Recombinant Mouse Cell cycle checkpoint control protein RAD9A (Rad9a)

  • 货号：
    CSB-YP896673MO
  • 规格：
  • 来源：
    Yeast
  • 其他：
  • 货号：
    CSB-EP896673MO
  • 规格：
  • 来源：
    E.coli
  • 其他：
  • 货号：
    CSB-EP896673MO-B
  • 规格：
  • 来源：
    E.coli
  • 共轭：
    Avi-tag Biotinylated

    E. coli biotin ligase (BirA) is highly specific in covalently attaching biotin to the 15 amino acid AviTag peptide. This recombinant protein was biotinylated in vivo by AviTag-BirA technology, which method is BriA catalyzes amide linkage between the biotin and the specific lysine of the AviTag.

  • 其他：
  • 货号：
    CSB-BP896673MO
  • 规格：
  • 来源：
    Baculovirus
  • 其他：
  • 货号：
    CSB-MP896673MO
  • 规格：
  • 来源：
    Mammalian cell
  • 其他：

产品详情

  • 纯度：
    >85% (SDS-PAGE)
  • 基因名：
  • Uniprot No.：
  • 别名：
    Rad9a; Rad9; Cell cycle checkpoint control protein RAD9A; mRAD9; EC 3.1.11.2; DNA repair exonuclease rad9 homolog A; Rad9-like protein
  • 种属：
    Mus musculus (Mouse)
  • 蛋白长度：
    full length protein
  • 表达区域：
    1-389
  • 氨基酸序列
    MKCLITGGNV KVLGKAVHSL SRIGDELYLE PLKDGLSLRT VNSSRSAYAC FLFAPLFFQQ YQAASPGQDL LRCKILMKAF LSVFRSLAIV EKSVEKCCIS LSGSHSHLVV QLHCKYGVKK THNLSFQDCE SLQAVFDPAS CPHLLRTPAR VLAEAVLSFP LALTEVTLGI GRGRRVILRS YQEEEADSTS KAMVTETSIG DEDFQQLHAP EGIAVTFCLK EFRGLLSFAE SANLPLTIHF DVPGRPVIFT IEDSLLDAHF VLATLLEQDS CSQGPCSPKP HQPVPQKQAH STPHLDDFTS DDIDCYMIAM ETTGGNEGSG AQPSTSLPPV SLASHDLAPT SEEEAEPSTV PGTPPPKKFR SLFFGSILAP VHSPQGPNPV LAEDSDGEG
  • 蛋白标签：
    Tag type will be determined during the manufacturing process.
    The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.
  • 产品提供形式：
    Lyophilized powder
    Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
  • 复溶：
    We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
  • 储存条件：
    Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
  • 保质期：
    The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
    Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
  • 货期：
    Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
    Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
  • 注意事项：
    Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
  • Datasheet ：
    Please contact us to get it.

产品评价

靶点详情

  • 功能：
    Component of the 9-1-1 cell-cycle checkpoint response complex that plays a major role in DNA repair. The 9-1-1 complex is recruited to DNA lesion upon damage by the RAD17-replication factor C (RFC) clamp loader complex. Acts then as a sliding clamp platform on DNA for several proteins involved in long-patch base excision repair (LP-BER). The 9-1-1 complex stimulates DNA polymerase beta (POLB) activity by increasing its affinity for the 3'-OH end of the primer-template and stabilizes POLB to those sites where LP-BER proceeds; endonuclease FEN1 cleavage activity on substrates with double, nick, or gap flaps of distinct sequences and lengths; and DNA ligase I (LIG1) on long-patch base excision repair substrates. The 9-1-1 complex is necessary for the recruitment of RHNO1 to sites of double-stranded breaks (DSB) occurring during the S phase. RAD9A possesses 3'->5' double stranded DNA exonuclease activity.
  • 基因功能参考文献：
    1. Rad9a is indispensable for spermatogonia differentiation and testicular development in mice. PMID: 27861152
    2. RAD9 has a prominent role in the ATR-Chk1 pathway that is necessary for successful formation of the damage-sensing complex and DNA damage checkpoint signaling. PMID: 26667770
    3. We demonstrated that Mrad9 null enhances chromatid aberration frequency induced by radiation in bystander mouse embryonic stem cells PMID: 25234738
    4. RAD9A is essential for male fertility and for repair of DNA double-strand breaks during meiotic prophase I. PMID: 23788429
    5. A review of the many activities assigned to Rad9, and speculation as to which influence its function in tumor development. PMID: 22034047
    6. Data show that Rad9 plays dual roles in generating functional antibodies and in maintaining the integrity of the whole genome in B cells. PMID: 20729201
    7. Rad9A-mediated Claspin localization is a vital step during checkpoint activation. PMID: 20081369
    8. tousled-like kinases play important roles in DNA repair, not only by modulation of chromatin assembly via Asf1, but also by a more direct function in processing the ends of a tousled-like kinase via interaction with Rad9. PMID: 20021694
    9. HRAD9 and Mrad9 are part of a gene family and reveal a new genetic element encoding a product that interacts with multiple, known cell cycle checkpoint control proteins. PMID: 14500360
    10. Results establish mouse rad9 as a key mammalian genetic element of pathways that regulate the cellular response to DNA damage, maintenance of genomic integrity, and proper embryonic development. PMID: 15282322
    11. The encoded mammalian proteins participate in promoting resistance to DNA damage, cell cycle checkpoint control, DNA repair, and apoptosis. PMID: 16365875
    12. Rad9 levels are high in prostate cancer cells due at least in part to aberrant methylation or gene amplification PMID: 18316588
    13. Mrad9 plays an important role in maintaining genomic stability and preventing tumor development in keratinocytes PMID: 18632607
    14. These results suggest that phosphorylation of Rad9 plays a critical role in the activation of the S phase/Mitosis checkpoint, and that downstream proteins cdc2 and 14-3-3sigma mediate this function. PMID: 18769057
    15. Rad9 is a signal in the earlier stage of epithelial cell cycle regulation and plays protective roles in alveolar epithelial cells in the pathogenesis of acute lung injury. PMID: 18809378
    16. Disruption of the Rad9-MLH1 interaction by a single-point mutation in Rad9 leads to significantly reduced DNA mismatch repair activity. PMID: 18842633

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  • 亚细胞定位：
    Nucleus.
  • 蛋白家族：
    Rad9 family
  • 组织特异性：
    Expressed in heart, brain, spleen, lung, liver, skeletal muscle, kidney and testis.
  • 数据库链接：

    KEGG: mmu:19367

    STRING: 10090.ENSMUSP00000025740

    UniGene: Mm.277629