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  4. Recombinant Mouse Alpha-defensin 5 (Defa5)

Recombinant Mouse Alpha-defensin 5 (Defa5)

In Stock
  • 货号：
    CSB-EP006657MO
  • 规格：
    ￥2328
  • 图片：
    • (Tris-Glycine gel) Discontinuous SDS-PAGE (reduced) with 5% enrichment gel and 15% separation gel.
  • 其他：

产品详情

  • 纯度：
    Greater than 90% as determined by SDS-PAGE.
  • 基因名：
    Defa5
  • Uniprot No.：
  • 别名：
    Defa5; Defcr5; Alpha-defensin 5; Defensin-related cryptdin-5
  • 种属：
    Mus musculus (Mouse)
  • 蛋白长度：
    Full Length of Mature Protein
  • 来源：
    E.coli
  • 分子量：
    31.2kDa
  • 表达区域：
    59-93aa
  • 氨基酸序列
    SKKLICYCRIRGCKRRERVFGTCRNLFLTFVFCCS
    Note: The complete sequence including tag sequence, target protein sequence and linker sequence could be provided upon request.
  • 蛋白标签：
    N-terminal GST-tagged
  • 产品提供形式：
    Liquid or Lyophilized powder
    Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
  • 缓冲液：
    Tris-based buffer,50% glycerol
  • 储存条件：
    Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
  • 保质期：
    The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
    Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
  • 货期：
    3-7 business days
  • 注意事项：
    Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
  • 产品描述：

    Recombinant Mouse Defa5 is an E.coli cell-expressed protein (Full Length of Mature Protein). E.coli expression systems are popular because bacteria are easy to culture, grow fast and produce high yields of recombinant protein. Traditional strategies for Mouse Defa5 recombinant protein expression involve transforming the recombinant plasmid into the E. coli host. The cells were directly replicated, transcribed and translated, and IPTG was added to express the desired protein. Typically, the cells are then lysed to extract the expressed protein for subsequent purification. Also, many proteins become insoluble as inclusion bodies that are very difficult to recover, so mild conditions and subsequent cumbersome protein-refolding procedures are used to ensure the final provided protein is soluble.

    Defa5 is a gene encoding a protein named alpha-defensin 5 which belongs to alpha-defensin family (also reffered to a family of microbicidal and cytotoxic peptides). The members of the defensin family are highly similar in protein sequence and distinguished by a conserved cysteine motif, which are involved in host defense, and help to maintain homeostasis of intestinal microbiota. Emerging evidence has reported that human alpha defensin 5 is highly expressed in secretory granules of crypt Paneth cells (PCs) of the small intestine (ileum) and is a candidate biomarker to delineate inflammatory bowel disease.

  • Datasheet & COA：
    Please contact us to get it.

产品评价

靶点详情

  • 功能：
    Probably contributes to the antimicrobial barrier function of the small bowel mucosa.
  • 亚细胞定位：
    Secreted.
  • 蛋白家族：
    Alpha-defensin family
  • 数据库链接：

    STRING: 10090.ENSMUSP00000093074

    UniGene: Mm.426634