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  4. Recombinant Mouse Alpha-defensin 4 (Defa4)

Recombinant Mouse Alpha-defensin 4 (Defa4)

  • 货号：
    CSB-YP006656MO
  • 规格：
  • 来源：
    Yeast
  • 其他：
  • 货号：
    CSB-EP006656MO
  • 规格：
  • 来源：
    E.coli
  • 其他：
  • 货号：
    CSB-EP006656MO-B
  • 规格：
  • 来源：
    E.coli
  • 共轭：
    Avi-tag Biotinylated

    E. coli biotin ligase (BirA) is highly specific in covalently attaching biotin to the 15 amino acid AviTag peptide. This recombinant protein was biotinylated in vivo by AviTag-BirA technology, which method is BriA catalyzes amide linkage between the biotin and the specific lysine of the AviTag.

  • 其他：
  • 货号：
    CSB-BP006656MO
  • 规格：
  • 来源：
    Baculovirus
  • 其他：
  • 货号：
    CSB-MP006656MO
  • 规格：
  • 来源：
    Mammalian cell
  • 其他：

产品详情

  • 纯度：
    >85% (SDS-PAGE)
  • 基因名：
    Defa4
  • Uniprot No.：
  • 别名：
    Defa4; Defcr4Alpha-defensin 4; Defensin-related cryptdin-4
  • 种属：
    Mus musculus (Mouse)
  • 蛋白长度：
    Cytoplasmic domain
  • 表达区域：
    59-92
  • 氨基酸序列
    LR GLLCYCRKGH CKRGERVRGT CGIRFLYCCP RR
  • 蛋白标签：
    Tag type will be determined during the manufacturing process.
    The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.
  • 产品提供形式：
    Lyophilized powder
    Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
  • 复溶：
    We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
  • 储存条件：
    Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
  • 保质期：
    The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
    Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
  • 货期：
    Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
    Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
  • 注意事项：
    Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
  • Datasheet ：
    Please contact us to get it.

产品评价

靶点详情

  • 功能：
    Probably contributes to the antimicrobial barrier function of the small bowel mucosa.
  • 基因功能参考文献：
    1. Depleting CRP4 is incompatible with a proper cardiac development and function. CRP4 is a novel antiremodeling factor regulated, at least to some extent, by cardiac cGMP. PMID: 28138039
    2. Backbone cyclization provides a means for engineeringalpha-defensins to improve their biological activity while providing excellent stability to serum degradation and highlighting the potential of peptide scaffolds for development of novel compounds. PMID: 22040603
    3. bactericidal activity predominantly affects noncommensal bacteria PMID: 21099205
    4. the prosegment of Crp4 inhibits Crp4 bactericidal activity by blocking peptide-mediated perturbation of target cell membranes PMID: 12574157
    5. Crp4 disulfide arrangement confers essential protection from degradation by this critical activating proteinase, allowing antibacterial action. PMID: 15297466
    6. function of the conserved salt bridge in Crp4 is not linked to bactericidal activity or proteolytic stability of the mature peptide. PMID: 16857681
    7. CRP4 mediates cGMP/PKG stimulation of SM-specific gene expression, and PKG plays an important role in regulating the phenotype of VSMCs PMID: 17878170
    8. Kinetics of Crp4 translocation coupled with peptide-induced vesicle leakage are reported. PMID: 17910476
    9. Beta-sheet-containing Crp4 induces membrane permeabilization more effectively than disulfide-null Crp4 by translocating and forming transient membrane defects. PMID: 18973303
    10. Lys-for-Arg replacements in cryptdin 4 attenuated bactericidal activity PMID: 19737896

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  • 亚细胞定位：
    Secreted.
  • 蛋白家族：
    Alpha-defensin family
  • 组织特异性：
    Paneth cells of the small bowel.
  • 数据库链接：

    KEGG: mmu:13238

    UniGene: Mm.426634