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  4. Mouse Neutrophil defensin 4(DEFA4) ELISA kit

Mouse Neutrophil defensin 4(DEFA4) ELISA kit

  • 中文名称：
    小鼠中性粒细胞防御素4(DEFA4)酶联免疫试剂盒
  • 货号：
    CSB-EL006656MO
  • 规格：
    96T/48T
  • 价格：
    ￥3600/￥2500
  • 其他：

产品详情

  • 产品描述：

    This Mouse DEFA4 ELISA Kit was designed for the quantitative measurement of Mouse DEFA4 protein in serum, plasma, tissue homogenates, cell lysates. It is a Sandwich ELISA kit, its detection range is 0.312 ng/mL-20 ng/mL and the sensitivity is 0.078 ng/mL.

  • 别名：
    Defa4 ELISA kit; Defcr4Alpha-defensin 4 ELISA kit; Defensin-related cryptdin-4 ELISA kit
  • 缩写：
    DEFA4
  • Uniprot No.：
  • 种属：
    Mus musculus (Mouse)
  • 样本类型：
    serum, plasma, tissue homogenates, cell lysates
  • 检测范围：
    0.312 ng/mL-20 ng/mL
  • 灵敏度：
    0.078 ng/mL
  • 反应时间：
    1-5h
  • 样本体积：
    50-100ul
  • 检测波长：
    450 nm
  • 研究领域：
    Immunology
  • 测定原理：
    quantitative
  • 测定方法：
    Sandwich
  • 精密度：
    Intra-assay Precision (Precision within an assay): CV%<8%
    Three samples of known concentration were tested twenty times on one plate to assess.
    Inter-assay Precision (Precision between assays): CV%<10%
    Three samples of known concentration were tested in twenty assays to assess.
  • 线性度：
    To assess the linearity of the assay, samples were spiked with high concentrations of mouse DEFA4 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
    　 Sample Serum(n=4)
    1:1 Average % 85
    Range % 81-89
    1:2 Average % 93
    Range % 90-96
    1:4 Average % 88
    Range % 84-92
    1:8 Average % 101
    Range % 97-105
  • 回收率：
    The recovery of mouse DEFA4 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.
    Sample Type Average % Recovery Range
    Serum (n=5) 99 95-104
    EDTA plasma (n=4) 83 80-86
  • 标准曲线：
    These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.
    ng/ml OD1 OD2 Average Corrected
    20 2.654 2.689 2.672 2.515
    10 2.059 2.001 2.030 1.873
    5 1.312 1.335 1.324 1.167
    2.5 0.852 0.868 0.860 0.703
    1.25 0.505 0.512 0.509 0.352
    0.625 0.373 0.360 0.367 0.210
    0.312 0.266 0.268 0.267 0.110
    0 0.158 0.156 0.157 　
  • 数据处理：
  • 货期：
    3-5 working days

产品评价

靶点详情

  • 功能：
    Probably contributes to the antimicrobial barrier function of the small bowel mucosa.
  • 基因功能参考文献：
    1. Depleting CRP4 is incompatible with a proper cardiac development and function. CRP4 is a novel antiremodeling factor regulated, at least to some extent, by cardiac cGMP. PMID: 28138039
    2. Backbone cyclization provides a means for engineeringalpha-defensins to improve their biological activity while providing excellent stability to serum degradation and highlighting the potential of peptide scaffolds for development of novel compounds. PMID: 22040603
    3. bactericidal activity predominantly affects noncommensal bacteria PMID: 21099205
    4. the prosegment of Crp4 inhibits Crp4 bactericidal activity by blocking peptide-mediated perturbation of target cell membranes PMID: 12574157
    5. Crp4 disulfide arrangement confers essential protection from degradation by this critical activating proteinase, allowing antibacterial action. PMID: 15297466
    6. function of the conserved salt bridge in Crp4 is not linked to bactericidal activity or proteolytic stability of the mature peptide. PMID: 16857681
    7. CRP4 mediates cGMP/PKG stimulation of SM-specific gene expression, and PKG plays an important role in regulating the phenotype of VSMCs PMID: 17878170
    8. Kinetics of Crp4 translocation coupled with peptide-induced vesicle leakage are reported. PMID: 17910476
    9. Beta-sheet-containing Crp4 induces membrane permeabilization more effectively than disulfide-null Crp4 by translocating and forming transient membrane defects. PMID: 18973303
    10. Lys-for-Arg replacements in cryptdin 4 attenuated bactericidal activity PMID: 19737896

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  • 亚细胞定位：
    Secreted.
  • 蛋白家族：
    Alpha-defensin family
  • 组织特异性：
    Paneth cells of the small bowel.
  • 数据库链接：

    KEGG: mmu:13238

    UniGene: Mm.426634