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  4. Recombinant Mouse 72 kDa type IV collagenase (Mmp2)

Recombinant Mouse 72 kDa type IV collagenase (Mmp2)

  • 货号：
    CSB-YP014666MO
  • 规格：
  • 来源：
    Yeast
  • 其他：
  • 货号：
    CSB-EP014666MO
  • 规格：
  • 来源：
    E.coli
  • 其他：
  • 货号：
    CSB-EP014666MO-B
  • 规格：
  • 来源：
    E.coli
  • 共轭：
    Avi-tag Biotinylated

    E. coli biotin ligase (BirA) is highly specific in covalently attaching biotin to the 15 amino acid AviTag peptide. This recombinant protein was biotinylated in vivo by AviTag-BirA technology, which method is BriA catalyzes amide linkage between the biotin and the specific lysine of the AviTag.

  • 其他：
  • 货号：
    CSB-BP014666MO
  • 规格：
  • 来源：
    Baculovirus
  • 其他：
  • 货号：
    CSB-MP014666MO
  • 规格：
  • 来源：
    Mammalian cell
  • 其他：

产品详情

  • 纯度：
    >85% (SDS-PAGE)
  • 基因名：
  • Uniprot No.：
  • 别名：
    Mmp2; 72 kDa type IV collagenase; EC 3.4.24.24; 72 kDa gelatinase; Gelatinase A; Matrix metalloproteinase-2; MMP-2) [Cleaved into: PEX]
  • 种属：
    Mus musculus (Mouse)
  • 蛋白长度：
    Full Length of Mature Protein
  • 表达区域：
    110-662
  • 氨基酸序列
    Y NFFPRKPKWD KNQITYRIIG YTPDLDPETV DDAFARALKV WSDVTPLRFS RIHDGEADIM INFGRWEHGD GYPFDGKDGL LAHAFAPGTG VGGDSHFDDD ELWTLGEGQV VRVKYGNADG EYCKFPFLFN GREYSSCTDT GRSDGFLWCS TTYNFEKDGK YGFCPHEALF TMGGNADGQP CKFPFRFQGT SYNSCTTEGR TDGYRWCGTT EDYDRDKKYG FCPETAMSTV GGNSEGAPCV FPFTFLGNKY ESCTSAGRND GKVWCATTTN YDDDRKWGFC PDQGYSLFLV AAHEFGHAMG LEHSQDPGAL MAPIYTYTKN FRLSHDDIKG IQELYGPSPD ADTDTGTGPT PTLGPVTPEI CKQDIVFDGI AQIRGEIFFF KDRFIWRTVT PRDKPTGPLL VATFWPELPE KIDAVYEAPQ EEKAVFFAGN EYWVYSASTL ERGYPKPLTS LGLPPDVQQV DAAFNWSKNK KTYIFAGDKF WRYNEVKKKM DPGFPKLIAD SWNAIPDNLD AVVDLQGGGH SYFFKGAYYL KLENQSLKSV KFGSIKSDWL GC
  • 蛋白标签：
    Tag type will be determined during the manufacturing process.
    The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.
  • 产品提供形式：
    Lyophilized powder
    Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
  • 复溶：
    We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
  • 储存条件：
    Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
  • 保质期：
    The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
    Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
  • 货期：
    Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
    Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
  • 注意事项：
    Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
  • Datasheet ：
    Please contact us to get it.

产品评价

靶点详情

  • 功能：
    Ubiquitinous metalloproteinase that is involved in diverse functions such as remodeling of the vasculature, angiogenesis, tissue repair, tumor invasion, inflammation, and atherosclerotic plaque rupture. As well as degrading extracellular matrix proteins, can also act on several nonmatrix proteins such as big endothelial 1 and beta-type CGRP promoting vasoconstriction. Also cleaves KISS at a Gly-|-Leu bond. Appears to have a role in myocardial cell death pathways. Contributes to myocardial oxidative stress by regulating the activity of GSK3beta. Cleaves GSK3beta in vitro. Involved in the formation of the fibrovascular tissues.; PEX, the C-terminal non-catalytic fragment of MMP2, posseses anti-angiogenic and anti-tumor properties and inhibits cell migration and cell adhesion to FGF2 and vitronectin. Ligand for integrin alpha-v/beta-3 on the surface of blood vessels.; Mediates the proteolysis of CHUK/IKKA and initiates a primary innate immune response by inducing mitochondrial-nuclear stress signaling with activation of the pro-inflammatory NF-kappaB, NFAT and IRF transcriptional pathways.
  • 基因功能参考文献：
    1. In obese mice, periodontitis caused the downregulation of MMP2, and upregulation of TIMP1 and TGF-beta1 at transcriptional and translational levels. PMID: 29322806
    2. In the initial periods of AP progression, an increased expression of MMP9 in the TLR2 KO and MyD88 KO mice was observed. In the final periods of AP progression, a reduction of MMP2 expression and an increase of MMP9 expression in the TLR2 KO mice were observed. MMP2 and MMP9 production was modulated for TLR2 and MyD88 during apical periodontitis progression PMID: 29267523
    3. Diet and exercise affect atheromatous MMP2/9 activity by modulating the systemic inflammatory milieu, with sVCAM-1, resistin, and adiponectin closely interacting with each other and with visceral fat. PMID: 28883215
    4. calpains inhibition plays crucial roles in vascular restenosis by preventing neointimal hyperplasia at the early stage via suppression of the MMP2/TGF-beta1 pathway. PMID: 27453531
    5. Aneurysmal-prone factors induced HIF-1alpha can cause overexpression of MMP-2 and MMP-9 and promote aneurysmal progression. PMID: 27363580
    6. These studies illustrated an important role of MMP2 in cognitive and motor behaviors and confirm its importance in NPC activities crucial to brain development, growth and response to and recovery from injury. PMID: 28666838
    7. Secretagogin-dependent MMP2 release from neurons regulates neuroblast migration. PMID: 28223495
    8. This novel mouse model will be a very useful tool for evaluating the mechanistic pathways and for development of novel therapies in cigarette smoke-associated lung emphysema. PMID: 29428733
    9. matrix metalloproteinase 2 (Mmp2) transcript is a target of miR-195a-3p, and that silencing Mmp2 phenocopied the reduced proliferation and migration of MSCs. The therapeutic potential of miR-195a-3p as an angiogenesis inhibitor was also demonstrated in a laser-induced choroidal neovascularization mouse model. PMID: 26989874
    10. developed a novel selective radiolabeled MMP2/9 inhibitor, suitable for single photon emission computed tomography (SPECT) imaging that effectively targets atherosclerotic lesions in mice PMID: 29190653
    11. MMP-2 and MMP-9 have roles in early stages of experimental autoimmune encephalomyelitis induction; MMP-9 from an immune cell source is required in EAE for initial infiltration of leukocytes into the central nervous system PMID: 27831901
    12. This study illustrated that tumour-derived MMP2 has at least two roles in tumour malignancy; to enhance tumour invasiveness by degrading the extracellular matrix and to enhance tumour growth by promoting vessel maturation and function. PMID: 29065106
    13. MMP-2 and -9 expression were suppressed significantly by treatment with SB-3CT. The data demonstrated, for the first time, that SB-3CT strongly reduced corneal lymphangiogenesis and macrophage infiltration during inflammation. PMID: 28669039
    14. animals were submitted to the evaluation of Blood-Brain Barrier permeability and MMP-2 and MMP-9 in striatum, hippocampus and cerebral cortex PMID: 27915985
    15. High MMP2 expression is associated with abdominal aortic aneurysm. PMID: 28179581
    16. Low MMP2 expression is associated with liver fibrosis. PMID: 28118605
    17. Cleavage of beta-DG still occurred when both MMP-2 and MMP-9 were knocked out in gamma - sarcoglycan-deficient mice. The study found that up-regulation of MMP-14 is capable of cleaving beta-DG, and it may be involved in the pathogenesis of sarcoglycanopathy. PMID: 28821434
    18. NH2-terminal truncated MMP-2 "primes" the kidney to enhanced susceptibility to I-R injury via induction of mitochondrial dysfunction. PMID: 28331061
    19. Study demonstrated evidence of beta-dystroglycan cleavage by matrix metalloproteinase-2/-9 in permanent middle cerebral artery occlusion mouse brains; this cleavage was implicated in aquaporin-4 redistribution and brain edema in cerebral ischemia. PMID: 27038751
    20. These results indicate that increased MMP2 and MMP9 activity in the brains of mouse adenovirus type 1-infected susceptible mice may be due to MMP activity produced by endothelial cells, astrocytes, and microglia, which in turn may contribute to blood-brain barrier disruption and encephalitis in susceptible mice. PMID: 28053109
    21. Studies define a novel HMGA1-MMP-2 pathway involved in a subset of human carcinosarcomas and tumor progression in murine models. PMID: 27001612
    22. activation of astrocyte MMP2/JNK1/2 contributes to the pathogenesis of pain hypersensitivity in the complex regional pain syndrome model PMID: 27919822
    23. Ceramide 1-phosphate -stimulated macrophage migration is a receptor mediated effect, and point to MMP-2 and -9 as possible therapeutic targets to control inflammation. PMID: 27164414
    24. MMP-2 potentiates shear-induced platelet activation by enhancing thrombus formation PMID: 26510894
    25. Identify novel MMP-2/cardiac sPLA2 pathway that endows the heart with important endocrine functions, including regulation of inflammation and lipid metabolism in the liver. PMID: 26567374
    26. 129/SvEv mice are more susceptible to abdominal aortic aneurysms compared to C57Bl/6 mice and suggest roles for MMP2/9. PMID: 26546710
    27. Report cross-talk between macrophages, smooth muscle cells, and endothelial cells in response to cigarette smoke alters MMP2/9 levels. PMID: 26318311
    28. MMP-2 silenced hypoxic fibroblasts under hyperglycemic conditions have impaired angiogenic potential. Collagen I/IV secretion is decreased and cell migration is prevented. PMID: 26985676
    29. N-terminal truncated isoform-MMP-2, but not full-length-MMP-2, is the major isoform of MMP-2 involved in skeletal muscle Ischemia-reperfusion injury. PMID: 26213293
    30. These data indicate that oxygen-glucose deprivation-triggered Cav-1 S-nitrosylation interacts with tPA-induced ERK activation to augment MMP2 and 9 secretion and subsequent extracellular matrix degradation. PMID: 26881424
    31. Type IV collagenases, MMP-2 and MMP-9, play important roles in hair cycle, and this could be mediated by induced expression of VEGF, IGF-1, and TGF-beta. PMID: 26451090
    32. Matrix Metalloproteinase-2 Knockout and Heterozygote Mice Are Protected from Hydronephrosis and Kidney Fibrosis after Unilateral Ureteral Obstruction PMID: 26673451
    33. Early MMP-2/MMP-9 activity is not a determinant of long-term recovery after traumatic brain injury in the immature mouse. PMID: 26588471
    34. Taken together, these findings indicate for the first time that AnxA2 phosphorylation and actin remodeling evoked by oxidative stress depend on the sphingolipid pathway, via MMP2 and p38MAPK. PMID: 25574848
    35. Matrix Metalloproteinase-2 (MMP-2) Gene Deletion Enhances MMP-9 Activity, Impairs PARP-1 Degradation, and Exacerbates Hepatic Ischemia and Reperfusion Injury in Mice PMID: 26355684
    36. OPN might be responsible for vascular remodeling diseases associated with hypertension by increasing MMP-2 production in vascluar smooth muscle cells. PMID: 25986148
    37. Further investigation of MMP2 inhibitors of TIMP2/TIMP4 showed an upregulated TIMP2 expression, but not TIMP4. low-dose pre-radiation attenuates the skin inflammation and ROS production induced by medium-dose UV radiation PMID: 26133107
    38. negatively regulates cardiac secreted phospholipase A2 to modulate inflammation and fever PMID: 25820137
    39. Data show that discoidin domain receptor (DDR) 2 siRNA-mediated suppression of extracellular regulated kinase (ERK) 1 and 2 and nuclear factor of kappa B (NF-kappaB) could down-regulate the expressions of matrix metalloproteinase (MMP) 2 and 9. PMID: 25733533
    40. Findings indicate the importance of MMP-2 in central nervous system development and dendritogenesis, and highlight the importance of a correct developmental wiring for adult brain morphology and function. PMID: 24652381
    41. Dietary supplementation with n-3 PUFAs may have protective anti-inflammatory effects mediated through modulation of MMPs and TIMPs PMID: 25512019
    42. Cytokine-induced MMP-2 activity specifically at the inflammatory border collectively act to accelerate leukocyte chemotaxis across the parenchymal border. PMID: 25704809
    43. MMP2/9 expression and activity are elevated in lacrimal glands of two murine models of Sjogren's syndrome, suggesting that manipulation of MMP2/9 activity might be a potential therapeutic target in chronically inflamed lacrimal glands. PMID: 26244298
    44. Data indicate that gelatinase A (MMP-2) deficient embryonic fibroblasts show reduced differentiation into adipocytes. PMID: 25869489
    45. beta-elemene downregulates expression of uPA, uPAR, MMP-2, and MMP-9 in a murine intraocular melanoma model PMID: 25405459
    46. Smoking equivalent levels of nicotine exposure induces VCAM-1, MMP-2, and MMP-9 expression. PMID: 25381636
    47. PDGF-D intensifies fibrogenesis by interfering with the fibrolytic activity of the TIMP-1/MMP-2/MMP-9 system, and PDGF-D signaling is mediated through both PDGF-alpha and -beta receptors. PMID: 25576870
    48. PPARdelta-mediated modulation of MMP-2 secretion and elastin expression may contribute to the maintenance of skin integrity by inhibiting ROS generation PMID: 25149191
    49. Study reveals the dual role of MMP2 in ECM degradation, as well as ECM synthesis in pathogenesis of thoracic aortic aneurysms. PMID: 25657308
    50. In conclusion, ATRA may increase expression of MMP-2 and MMP-9 by the potential signal pathway of RAR-alpha and RAR-gamma in injury podocyte induced by adriamycin, but not RAR-beta. PMID: 24694005

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  • 亚细胞定位：
    [Isoform 1]: Secreted, extracellular space, extracellular matrix. Membrane. Nucleus.; [Isoform 2]: Cytoplasm. Mitochondrion.
  • 蛋白家族：
    Peptidase M10A family
  • 数据库链接：

    KEGG: mmu:17390

    STRING: 10090.ENSMUSP00000034187

    UniGene: Mm.29564