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  4. Recombinant Human herpesvirus 1 ICP47 protein (US12)

Recombinant Human herpesvirus 1 ICP47 protein (US12)

  • 货号：
    CSB-YP360979HWY
  • 规格：
    ￥2616
  • 图片：
    • (Tris-Glycine gel) Discontinuous SDS-PAGE (reduced) with 5% enrichment gel and 15% separation gel.
  • 其他：

产品详情

  • 纯度：
    Greater than 90% as determined by SDS-PAGE.
  • 基因名：
    US12
  • Uniprot No.：
  • 别名：
    US12; ICP47 protein; Immediate-early protein IE12; Immediate-early-5; Infected cell protein 47; US12 protein; Vmw12
  • 种属：
    Human herpesvirus 1 (strain 17) (HHV-1) (Human herpes simplex virus 1)
  • 蛋白长度：
    Full Length
  • 来源：
    Yeast
  • 分子量：
    11.8kDa
  • 表达区域：
    1-88aa
  • 氨基酸序列
    MSWALEMADTFLDTMRVGPRTYADVRDEINKRGREDREAARTAVHDPERPLLRSPGLLPEIAPNASLGVAHRRTGGTVTDSPRNPVTR
    Note: The complete sequence including tag sequence, target protein sequence and linker sequence could be provided upon request.
  • 蛋白标签：
    N-terminal 6xHis-tagged
  • 产品提供形式：
    Liquid or Lyophilized powder
    Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
  • 缓冲液：
    Tris-based buffer,50% glycerol
  • 储存条件：
    Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
  • 保质期：
    The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
    Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
  • 货期：
    Basically, we can dispatch the products out in 1-3 working days after receiving your orders. Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
    Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
  • 注意事项：
    Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
  • 产品描述：

    The fusion tag N-terminal 6xHis tag gene was added to the gene sequence corresponding to the yeast of the HHV-1 US12 protein to form the recombinant DNA. The recombinant DNA was cloned into the expression vector and then transfected into the yeast cells for expression. Following purification, the product is the recombinant HHV-1 US12 protein carrying N-terminal 6xHis tag. The SDS-PAGE assessed the purity of this recombinant US12 protein up to 90%. It had an apparent molecular weight of approximately 14 kDa.

    US12 is gene providing instructions for making a protein named human herpesvirus 1 ICP47 protein (abbreviated US12) and belongs to Herpesviridae US12 family. US12, a HSV immediate early protein, is small protein produced by the herpes simplex virus, is considered as an important factor in the evasion of cellular immune responses in HSV-infected cells. It is expressed very early after infection and can inhibit CD8+ T cell recognition of infected cells. Recent studies have established clearly that ICP47 can bind with high affinity to human TAP and inhibit the transport of peptides into the endoplasmic reticulum.

  • Datasheet & COA：
    Please contact us to get it.

产品评价

靶点详情

  • 功能：
    Plays a role in the inhibition of host immune response. Binds specifically to transporters associated with antigen processing (TAP), thereby blocking peptide-binding and translocation by TAP as well as subsequent loading of peptides onto MHC class I molecules. Empty MHC I molecules are retained in the endoplasmic reticulum and ultimately directed to proteasomal degradation. In consequence, infected cells are masked for immune recognition by cytotoxic T-lymphocytes.
  • 基因功能参考文献：
    1. The structure shows that herpes simplex virus ICP47 traps human TAP in an inactive conformation distinct from the normal transport cycle. PMID: 27935481
    2. cryo-electron microscopy structure of human TAP in complex with its inhibitor ICP47, a small protein produced by the herpes simplex virus I PMID: 26789246
    3. Results indicate that recombinant adenovirus r-H-ICP47 had the abilities of reducing the proliferation of lymphocytes activated by dendritic cells (DCs). PMID: 23774531
  • 亚细胞定位：
    Host cytoplasm. Host nucleus.
  • 蛋白家族：
    Herpesviridae US12 family
  • 数据库链接：

    KEGG: vg:2703441