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  4. Recombinant Human Vitamin K-dependent protein C (PROC)

Recombinant Human Vitamin K-dependent protein C (PROC)

  • 货号：
    CSB-YP018742HU
  • 规格：
  • 来源：
    Yeast
  • 其他：
  • 货号：
    CSB-EP018742HU-B
  • 规格：
  • 来源：
    E.coli
  • 共轭：
    Avi-tag Biotinylated

    E. coli biotin ligase (BirA) is highly specific in covalently attaching biotin to the 15 amino acid AviTag peptide. This recombinant protein was biotinylated in vivo by AviTag-BirA technology, which method is BriA catalyzes amide linkage between the biotin and the specific lysine of the AviTag.

  • 其他：
  • 货号：
    CSB-BP018742HU
  • 规格：
  • 来源：
    Baculovirus
  • 其他：
  • 货号：
    CSB-MP018742HU
  • 规格：
  • 来源：
    Mammalian cell
  • 其他：

产品详情

  • 纯度：
    >85% (SDS-PAGE)
  • 基因名：
  • Uniprot No.：
  • 别名：
    Activation peptide; Anticoagulant protein C; APC; Autoprothrombin IIA; Blood coagulation factor XIV; EC 3.4.21.69; PC; proC; PROC_HUMAN; PROC1 ; Protein C (inactivator of coagulation factors Va and VIIIa); THPH3; THPH4; Vitamin K dependent protein C
  • 种属：
    Homo sapiens (Human)
  • 蛋白长度：
    Full Length of Mature Protein
  • 表达区域：
    43-461
  • 氨基酸序列
    ANSFLEEL RHSSLERECI EEICDFEEAK EIFQNVDDTL AFWSKHVDGD QCLVLPLEHP CASLCCGHGT CIDGIGSFSC DCRSGWEGRF CQREVSFLNC SLDNGGCTHY CLEEVGWRRC SCAPGYKLGD DLLQCHPAVK FPCGRPWKRM EKKRSHLKRD TEDQEDQVDP RLIDGKMTRR GDSPWQVVLL DSKKKLACGA VLIHPSWVLT AAHCMDESKK LLVRLGEYDL RRWEKWELDL DIKEVFVHPN YSKSTTDNDI ALLHLAQPAT LSQTIVPICL PDSGLAEREL NQAGQETLVT GWGYHSSREK EAKRNRTFVL NFIKIPVVPH NECSEVMSNM VSENMLCAGI LGDRQDACEG DSGGPMVASF HGTWFLVGLV SWGEGCGLLH NYGVYTKVSR YLDWIHGHIR DKEAPQKSWA P
  • 蛋白标签：
    Tag type will be determined during the manufacturing process.
    The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.
  • 产品提供形式：
    Lyophilized powder
    Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
  • 复溶：
    We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
  • 储存条件：
    Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
  • 保质期：
    The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
    Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
  • 货期：
    Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
    Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
  • 注意事项：
    Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
  • Datasheet ：
    Please contact us to get it.

产品评价

靶点详情

  • 功能：
    Protein C is a vitamin K-dependent serine protease that regulates blood coagulation by inactivating factors Va and VIIIa in the presence of calcium ions and phospholipids. Exerts a protective effect on the endothelial cell barrier function.
  • 基因功能参考文献：
    1. The fusion protein recapitulates the functional and structural properties of the thrombin-thrombomodulin complex, prolongs the clotting time by generating pharmacological quantities of activated protein C and effectively diagnoses protein C deficiency in human plasma. PMID: 28294177
    2. Polymorphisms of F2, PROC, PROZ, and F13A1 Genes are Associated With Recurrent Spontaneous Abortion in Chinese Han Women. PMID: 29363996
    3. Laboratory tests and direct sequencing of PROC and SERPINC1 were conducted for the patient and his family members. Coagulation tests revealed that the patient presented type I antithrombin deficiency combined with decreased protein C activity resulting from a small insertion mutation c.848_849insGATGT in SERPINC1 and a short deletion variant c.572_574delAGA in PROC. PMID: 28861852
    4. Patients with rheumatoid arthritis, polymyositis, systemic lupus erythematosus and ankylosing spondylitis showed higher serum levels of than healthy control individuals. PMID: 29303701
    5. Using protein C-factor VII chimera demonstrate that APC light chain amino acid residues outside the EPCR-binding site enable cytoprotective PAR1 signaling. PMID: 28834159
    6. We recently identified a venous thrombosis patient whose plasma level of protein C antigen is normal, but its anticoagulant activity is only 34 % of the normal level. Genetic analysis revealed that the proband and her younger brother carry a novel heterozygous mutation c.346G>A, p.Gly74Ser (G74S) in PROC. PMID: 28405673
    7. Developed functional protein S assays that measure both the activated protein C- and TFPI-cofactor activities of protein S in plasma, which are hardly if at all affected by the FV Leiden mutation. PMID: 28211163
    8. Protein C seems inversely associated with mortality in patients with advanced prostate, lung and pancreatic cancer. Further research should validate protein C as a biomarker for mortality, and explore the effects of protein C on progression of cancer. PMID: 28376316
    9. Genetically higher protein C was associated with a lower risk of ischaemic heart disease. PMID: 27882376
    10. EPCR occupancy recruits G-protein coupled receptor kinase 5, thereby inducing beta-arrestin-2 biased PAR1 signaling by both APC and thrombin. In PMID: 27561318
    11. A novel mutation of A309V in PROC was determined in a family of two patients with late onset protein C deficiency PMID: 28111891
    12. loss of the convertase furin or PC5/6 in hepatocytes results in a approximately 30% decrease in APC levels, with no significant contribution from PACE4. We conclude that prior convertase cleavage of protein C in hepatocytes is critical for its thrombin activation. PMID: 28468828
    13. Both probands had hereditary protein C deficiencies, for which their parents were all carriers. PMID: 28186585
    14. Variants influencing circulating protein C levels in the CELSR2-PSRC1-SORT1 region may indicate a novel genetic link between lipoprotein metabolism and hemostasis. PMID: 28082259
    15. These findings suggest that the anti-inflammatory function of APC at therapeutic concentrations may include the inhibition of NETosis in an EPCR-, PAR3-, and Mac-1-dependent manner, providing additional mechanistic insight into the diverse functions of neutrophils and APC in disease states including sepsis. PMID: 28408624
    16. Collectively, these data depict a pivotal role for AMPK signaling in inhibiting ER stress responses via the activation of APC during MGO-induced cardiomyocyte apoptosis. Thus, APC may be a potential novel therapeutic target for the management of diabetic cardiovascular complications such as diabetic cardiomyopathy. PMID: 27794481
    17. An activation-resistant homozygous protein C R229W mutation was linked to perinatal intracranial bleeding and delayed onset of thrombosis. PMID: 27172833
    18. Structural and functional studies of novel heterozygous mutations in the PROC gene confirmed that the mutations were pathogenetic in two Chinese families with types I and II protein C deficiency. PMID: 27517348
    19. The odds ratio of developing idiopathic fatal pulmonary embolism as a variant carrier for PROC is 85.6 (95% CI, 13.0-448.9; P = 2.0 x 10- 5. PMID: 28174134
    20. Study detected a statistically significant positive correlation between expanded disability status scale scores and thrombomodulin levels (p<0.01) and a 10% positive correlation between expanded disability status scale scores and APC levels in multiple sclerosis patients PMID: 27456888
    21. Low protein C expression is associated with diabetes mellitus. PMID: 27345772
    22. Genetic variants in thrombomodulin and endothelial protein C receptor genes are additively associated with mortality in acute respiratory distress syndrome in European ancestry PMID: 27215212
    23. Protein C and TAFI are concurrently activated in a thrombomoulin-dependent manner and do not compete for the thrombin-TM complex, raising the possibility that they interact with distinct activation complexes. PMID: 26663133
    24. We herewith present the first case of fetal ventriculomegaly and neonatal stroke associated with heterozygous PROC mutation. PMID: 26250584
    25. Elevated levels of circulating microparticles can play a role in carriers of mild and severe inherited thrombophilia resulting from protein C deficiency. PMID: 26354831
    26. deficiency associated with venous thromboembolism in pregnancy PMID: 25586317
    27. ApoER2 contributes cooperatively with endothelial cell protein C receptor and protease activated receptor 1 to APC-initiated endothelial antiapoptotic and barrier protective signaling. PMID: 26800564
    28. Results show that free activated protein C binds to membrane EPCR in ovarian cancer cells inducing cell migration via MEK-ERK and Rho-GTPase pathways. The cancer cells become highly aggressive, and secondary nodules develop because of fibrin inhibition. PMID: 26082331
    29. Low levels of protein C during pregnancy were not associated with adverse pregnancy outcome. PMID: 25879167
    30. Report activation of protein C and down-regulation of EPCR in trophobolasts stimulated with TNF-alpha. PMID: 25667200
    31. levels of protein C and soluble thrombomodulin in critically ill patients with acute kidney injury PMID: 25790110
    32. We conclude that the Cohn process significantly influences the anticoagulant activity of PC. Compared to the antigen, PC lost greater than 80% of its anticoagulant activity, but retained its amidolytic activity, during the Cohn process. PMID: 25730025
    33. Data show the molecular model of the complex of activated protein C (APC) with alphaVbeta3 integrin obtained by protein-protein docking approach. PMID: 24251463
    34. Our findings identify PROC on 2q14 as a novel candidate for childhood asthma PMID: 25643747
    35. Protein C is activated during in vitro thrombolysis. PMID: 26552309
    36. Results provide evidence that APC selectively inhibits proliferation and the inflammatory signaling pathways of rheumatoid synovial fibroblasts. PMID: 24096826
    37. High avidity anti-protein C antibodies by provide marker for a severe thrombotic phenotype in antiphospholipid syndrome. PMID: 25196808
    38. A single measurement of protein C was able to identify severe coagulopathy early in the course of sepsis. PMID: 24410881
    39. Suggest that PC deficiency is an independent predictor for the progression of critical limb ischemia. PMID: 25115555
    40. Hereditary protein C deficiency in a family with venous thrombosis was associated with two missense mutations in the PROC gene. PMID: 25748729
    41. Heterozygous F139V mutations caused mild reduction of protein C function, which might be the reason for survival of compound heterozygous or homozygous protein C deficiency adults carrying these mutations. PMID: 24911457
    42. A novel PC mutation in a healthy subject results in type II PC deficiency as diagnosed by commercial kits. PMID: 25651845
    43. Dialysis patients who are carriers of the AG/GG genotypes of the PROC rs1799809 had a 1.2-fold increased 5-year all-cause mortality risk. PMID: 24816905
    44. The study reports effects of the prothrombin Yukuhashi mutation on thrombomodulin-protein C anticoagulant system. PMID: 25149909
    45. observed increased APC resistance in carriers of fibrinogen gamma gene (FGG) haplotype 2, which is associated with reduced levels of the alternatively spliced fibrinogen gamma' chain. We then studied the effects of fibrinogen and its gamma' chain on APC resistance. PMID: 24951429
    46. Although platelet fVa shows slightly superior resistance to aPC's effects compared to plasma fVa, neither fVa is sufficiently cleaved in simulations of acute traumatic coagulopathy or pharmacologically-delivered aPC to diminish coagulation parameters. PMID: 24921658
    47. The inflammation-induced activation of the protein C pathway provides negative feedback inhibition of coagulation and exerts coagulation-independent anti-inflammatory and cytoprotective effects. PMID: 24759138
    48. Dysfunction of protein C in Japanese and Chinese in this review is significantly associated with increased risk for venous thromboembolism. PMID: 24233386
    49. The PROC TAA haplotype was associated with a high protein C level and increased risk of venous thrombosis. PMID: 24158116
    50. Decreased APC levels in patients with type 2 diabetes may serve as a biomarker to indicate the early development of diabetic nephropathy. PMID: 24660539

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  • 相关疾病：
    Thrombophilia due to protein C deficiency, autosomal dominant (THPH3); Thrombophilia due to protein C deficiency, autosomal recessive (THPH4)
  • 亚细胞定位：
    Secreted. Golgi apparatus. Endoplasmic reticulum.
  • 蛋白家族：
    Peptidase S1 family
  • 组织特异性：
    Plasma; synthesized in the liver.
  • 数据库链接：

    HGNC: 9451

    OMIM: 176860

    KEGG: hsa:5624

    STRING: 9606.ENSP00000234071

    UniGene: Hs.224698