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  4. Recombinant Human Transcription regulator protein BACH1 (BACH1)

Recombinant Human Transcription regulator protein BACH1 (BACH1)

  • 货号：
    CSB-YP002526HU
  • 规格：
  • 来源：
    Yeast
  • 其他：
  • 货号：
    CSB-EP002526HU
  • 规格：
  • 来源：
    E.coli
  • 其他：
  • 货号：
    CSB-EP002526HU-B
  • 规格：
  • 来源：
    E.coli
  • 共轭：
    Avi-tag Biotinylated

    E. coli biotin ligase (BirA) is highly specific in covalently attaching biotin to the 15 amino acid AviTag peptide. This recombinant protein was biotinylated in vivo by AviTag-BirA technology, which method is BriA catalyzes amide linkage between the biotin and the specific lysine of the AviTag.

  • 其他：
  • 货号：
    CSB-BP002526HU
  • 规格：
  • 来源：
    Baculovirus
  • 其他：
  • 货号：
    CSB-MP002526HU
  • 规格：
  • 来源：
    Mammalian cell
  • 其他：

产品详情

  • 纯度：
    >85% (SDS-PAGE)
  • 基因名：
  • Uniprot No.：
  • 别名：
    BACH 1; BACH 1.3; Bach1; BACH1_HUMAN; Basic leucine zipper transcription factor 1; basic region leucine zipper transcriptional regulator BACH1; BTB and CNC homolog 1; BTB and CNC homology 1; BTB and CNC homology 1; basic leucine zipper transcription factor 1; HA2303; OTTHUMP00000096563; OTTHUMP00000096564; Transcription regulator protein BACH1
  • 种属：
    Homo sapiens (Human)
  • 蛋白长度：
    Full length protein
  • 表达区域：
    1-736
  • 氨基酸序列
    MSLSENSVFA YESSVHSTNV LLSLNDQRKK DVLCDVTIFV EGQRFRAHRS VLAACSSYFH SRIVGQADGE LNITLPEEVT VKGFEPLIQF AYTAKLILSK ENVDEVCKCV EFLSVHNIEE SCFQFLKFKF LDSTADQQEC PRKKCFSSHC QKTDLKLSLL DQRDLETDEV EEFLENKNVQ TPQCKLRRYQ GNAKASPPLQ DSASQTYESM CLEKDAALAL PSLCPKYRKF QKAFGTDRVR TGESSVKDIH ASVQPNERSE NECLGGVPEC RDLQVMLKCD ESKLAMEPEE TKKDPASQCP TEKSEVTPFP HNSSIDPHGL YSLSLLHTYD QYGDLNFAGM QNTTVLTEKP LSGTDVQEKT FGESQDLPLK SDLGTREDSS VASSDRSSVE REVAEHLAKG FWSDICSTDT PCQMQLSPAV AKDGSEQISQ KRSECPWLGI RISESPEPGQ RTFTTLSSVN CPFISTLSTE GCSSNLEIGN DDYVSEPQQE PCPYACVISL GDDSETDTEG DSESCSAREQ ECEVKLPFNA QRIISLSRND FQSLLKMHKL TPEQLDCIHD IRRRSKNRIA AQRCRKRKLD CIQNLESEIE KLQSEKESLL KERDHILSTL GETKQNLTGL CQKVCKEAAL SQEQIQILAK YSAADCPLSF LISEKDKSTP DGELALPSIF SLSDRPPAVL PPCARGNSEP GYARGQESQQ MSTATSEQAG PAEQCRQSGG ISDFCQQMTD KCTTDE
  • 蛋白标签：
    Tag type will be determined during the manufacturing process.
    The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.
  • 产品提供形式：
    Lyophilized powder
    Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
  • 复溶：
    We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
  • 储存条件：
    Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
  • 保质期：
    The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
    Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
  • 货期：
    Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
    Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
  • 注意事项：
    Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
  • Datasheet ：
    Please contact us to get it.

产品评价

靶点详情

  • 功能：
    Transcriptional regulator that acts as repressor or activator, depending on the context. Binds to NF-E2 DNA binding sites. Plays important roles in coordinating transcription activation and repression by MAFK. Together with MAF, represses the transcription of genes under the control of the NFE2L2 oxidative stress pathway.
  • 基因功能参考文献：
    1. BACH1 is overexpressed in prostate cancer PMID: 28889753
    2. BACH1 may inhibit the progression of colorectal cancer through BACH1/CXCR4 pathway. PMID: 29481800
    3. BACH1 overexpression impaired the association between p53 and SP1 via competitive binding p53, and antagonized the impact of p53 on MGMT expression. PMID: 28000777
    4. In light of a pivotal role of NRF2 and BACH1 in response to oxidative stress and regulation of HO-1, we examined if smoke-induced HO-1 expression is modulated through the NRF2/BACH1 axis. We demonstrated that smoke causes significant nuclear translocation of NRF2, but only a slight decrease in nuclear BACH1. PMID: 29125538
    5. these findings suggested that increased miR-155 expression in activated monocytes leads to enhanced phagocytic activity via BACH-1 regulation in beta-thalassemia/HbE. PMID: 28685309
    6. The electrophilic character of quinones ensure their conjugation with Bach1, which is important for the downregulation of Bach1 and the upregulation of Nrf2 signaling. PMID: 28645578
    7. Our results suggest that the bach1-specific small interfering RNA effectively decrease CXCR4 receptor, matrix metalloproteinase-9 expression and breast adenocarcinoma cells invasive, also increased the expression of tumor-suppressive microRNA-203 and miR-145 PMID: 28349828
    8. BACH1 down-regulation in HT29 colon cancer cells had no effect on cell growth but did inhibit cell migration by decreasing metastasis-related genes expression. PMID: 27657827
    9. This study indicates that the expression of BACH1 could be elevated as a compensatory mechanism to decrease the globin chain imbalance as well as to reduce the oxidative stress found in hemoglobin E/beta-thalassemia. PMID: 26377036
    10. A short while ago some studies suggested that BACH1 is involved in cancers, especially breast cancer. This factor is mentioned as a novel master regulator which adjusts several genes involved in bone metastasis of breast cancer, especially CXCR4 and MMP1, two main genes in the enhancement of cancer cell migration and invasion to distant organs. [Review] PMID: 27108804
    11. Bach1 suppresses cell proliferation and induces cell-cycle arrest and apoptosis. PMID: 27057283
    12. Induction of GSH-related genes xCT and GCLM were oxygen and Bach1-insensitive during long-term culture under 5% O2, providing the first evidence that genes related to GSH synthesis mediate protection afforded by Nrf2-Keap1 defense pathway PMID: 26698668
    13. heme oxygenase-1 expression is induced by gold nanoparticles through Nrf2 activation and Bach1 export in human vascular endothelial cells PMID: 26445536
    14. Cyanidin-3-O-glucoside protects HUVECs from palmitic acid-induced injuryby modulating the balance of Nrf2 versus Bach1 inside the nucleus so influencing upregulation of electrophile responsive element mediated gene expression. PMID: 26422990
    15. sensitizer-induced up-regulation of both the endogenous HMOX1 and the luciferase constructs under the control of the HMOX1-ARE or the full HMOX1 promoter appear to be under the control of both Nrf2 and Bach1. PMID: 26244607
    16. This study demonstrated that Bach1 overexpression in Down syndrome correlates with the alteration of the HO-1/BVR-a system. PMID: 25391381
    17. Bach1 suppresses angiogenesis after ischemic injury and impairs Wnt/beta-catenin signaling by disrupting the interaction between beta-catenin and TCF4 and by recruiting histone deacetylase 1 to the promoter of TCF4-targeted genes. PMID: 26123998
    18. Higher HMOX1 expression correlated with higher expression of Bach-1 (Spearman's rho = 0.586, p = 0.000001) and miR-122 (Spearman's rho = 0.270, p = 0.014059). PMID: 24752012
    19. The Bach1-dependent repression of the HO-1 expression is under the control of the Hx-dependent uptake of extracellular heme PMID: 24613679
    20. The arsenic-induced Nrf2 pathway activation in hepatocytes suggested that the translocation of Bach1 was associated with the regulation of Nrf2 pathway by arsenic. PMID: 23738048
    21. In colorectal cancer, Nrf2 expression is closely correlated with Keap1 in the tumor and with Bach1 in the normal tissue. PMID: 23455180
    22. CXCR3-B mediates a growth-inhibitory signal in breast cancer cells through the modulations of nuclear translocation of Bach-1 and Nrf2 and down-regulation of HO-1. PMID: 24366869
    23. BACH1 acts in a double-negative (overall positive) feedback loop to inhibit RKIP transcription in breast cancer cells PMID: 24395801
    24. Identify a role for SCF(FBXL17) in controlling the threshold for NRF2-dependent gene activation via BACH1 repressor turnover. PMID: 24035498
    25. Data indicate that transcription factors MafK and Bach1 regulate expression of heme oxygenase-1 (HO-1). PMID: 23737527
    26. let-7 miRNA directly acts on the 3'-UTR of Bach1 and negatively regulates expression of this protein, and thereby up-regulates HMOX1 gene expression. PMID: 22698995
    27. miR-155 may function as an oncogene by targeting BACH1 PMID: 22307849
    28. Bach1 depletion resulted in disordered mitotic chromosome alignment, which was rescued by Bach1 mutants lacking the BTB or DNA binding domains, suggesting its transcription-independent mechanism. PMID: 22289179
    29. We conclude that BACH1 is a bona fide Nrf2 target gene and that induction of BACH1 by Nrf2 may serve as a feedback-inhibitory mechanism for antioxidant-response-element-mediated gene regulation. PMID: 21812759
    30. The BACH1 target genes in HEK 293 cells are involved in heme degradation, redox regulation, cell cycle/apoptosis pathways and subcellular transport processes. PMID: 21555518
    31. The BTB and CNC homology 1 (BACH1) target genes are involved in the oxidative stress response and in control of the cell cycle. PMID: 21555518
    32. Bach1 plays a critical role in regulating HO-1 gene expression in AML cells and its expression suppresses their survival by downregulating HO-1 expression. PMID: 20345481
    33. prevents radiation-induced upregulation of heme oxygenase 1 gene expression in keratinocytes PMID: 19874887
    34. miR-196 directly acts on the 3'-UTR of Bach1 messenger RNA and translationally represses the expression of this protein. PMID: 20127796
    35. Bach1 functions as a hypoxia-inducible repressor for the HO-1 gene, thereby contributing to fine-tuning of oxygen homeostasis in human cells PMID: 12511571
    36. nuclear export of Bach1 constitutes an important regulatory mechanism to relieve the Bach1-mediated repression of genes such as heme oxygenase 1 PMID: 14504288
    37. Bach1 binding to the Maf recognition element in the microlocus control region is blocked by heme in the beta-globin regulation pathway PMID: 14660636
    38. BACH1 plays a role in the development of Alzheimer's disease (AD)-like neuropathology in Down syndrome and in pathogenesis of AD per se. PMID: 15068237
    39. BACH1 protein is significantly overexpressed in fetal Down syndrome cerebral cortex and may contribute to abnormal brain development or at least to defective transcription machinery in Down syndrome. PMID: 15068251
    40. These results indicated that heme plays an important role in the induction of alpha-globin gene expression through disrupting the interaction of Bach1 and the NA site in HS-40 enhancer in erythroid cells. PMID: 15464985
    41. Bach-1 has a specific and selective ability to repress expression of hepatic heme oxygenase-1 PMID: 15465821
    42. BACH1 acts as a transcriptional repressor in the regulation of Maf recognition-element-dependent genes in megakaryocytes. PMID: 15613547
    43. key role of down-regulation of Bach1 and up-regulation of heme oxygenase 1 in diminishing cytotoxic effects of hepatitis C virus proteins in human hepatocytes. PMID: 16530877
    44. Reviewer presents the Bach1-heme oxygenase(HO)-1 system as an important defense mechanism against oxidative stress--a mechanism in which Bach1 is a critical regulator of HO-1. PMID: 16724942
    45. Data show that beta-carotene, combined with cigarette smoke condensate (TAR), regulates heme oxygenase-1 (HO-1) via its transcriptional factor Bach1 and modulates cell growth. PMID: 16771696
    46. The effect of Bach1 and Nrf2 on heme oxygenase 1 expression via cobalt protoporphyrin in human liver cells is reported. PMID: 17065227
    47. BACH-1 protein levels are low in cells expressing either the human miR-155 or miR-K12-11 of Kaposi's sarcoma-associated herpesvirus. PMID: 17881434
    48. Loss of BACH1 function in human keratinocytes results almost exclusively in HMOX1 induction PMID: 18550526
    49. Bach1 inhibits expression of oxidative stress responsive genes by competing with Nrf2, the key activator of oxidative stress response. Bach1 inhibits p53-dependent cellular senescence induced by oxidative stress PMID: 19591297
    50. regulates oxidative stress response in cellular senescence. (review) PMID: 19618874

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  • 亚细胞定位：
    Nucleus.
  • 蛋白家族：
    BZIP family, CNC subfamily
  • 数据库链接：

    HGNC: 935

    OMIM: 602751

    KEGG: hsa:571

    STRING: 9606.ENSP00000286800

    UniGene: Hs.154276