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  4. Recombinant Human Thymidine phosphorylase (TYMP)

Recombinant Human Thymidine phosphorylase (TYMP)

  • 货号：
    CSB-YP025392HU
  • 规格：
  • 来源：
    Yeast
  • 其他：
  • 货号：
    CSB-EP025392HU
  • 规格：
  • 来源：
    E.coli
  • 其他：
  • 货号：
    CSB-EP025392HU-B
  • 规格：
  • 来源：
    E.coli
  • 共轭：
    Avi-tag Biotinylated

    E. coli biotin ligase (BirA) is highly specific in covalently attaching biotin to the 15 amino acid AviTag peptide. This recombinant protein was biotinylated in vivo by AviTag-BirA technology, which method is BriA catalyzes amide linkage between the biotin and the specific lysine of the AviTag.

  • 其他：
  • 货号：
    CSB-BP025392HU
  • 规格：
  • 来源：
    Baculovirus
  • 其他：
  • 货号：
    CSB-MP025392HU
  • 规格：
  • 来源：
    Mammalian cell
  • 其他：

产品详情

  • 纯度：
    >85% (SDS-PAGE)
  • 基因名：
    TYMP
  • Uniprot No.：
  • 别名：
    ECGF 1; ECGF; ECGF1; Endothelial cell growth factor 1; Endothelial cell growth factor 1 platelet derived; Endothelial cell growth factor, platelet-derived; Gliostatin; hPD ECGF; MEDPS1; MNGIE; MTDPS1; PD ECGF; PD-ECGF; PDECGF; PDEGF; Platelet derived endothelial cell growth factor; Platelet derived endothelial growth factor; Platelet-derived endothelial cell growth factor; TdRPase; Thymidine phosphorylase; TP; Tymp; TYPH_HUMAN
  • 种属：
    Homo sapiens (Human)
  • 蛋白长度：
    Full length protein
  • 表达区域：
    11-482
  • 氨基酸序列
    APPAPGDFSG EGSQGLPDPS PEPKQLPELI RMKRDGGRLS EADIRGFVAA VVNGSAQGAQ IGAMLMAIRL RGMDLEETSV LTQALAQSGQ QLEWPEAWRQ QLVDKHSTGG VGDKVSLVLA PALAACGCKV PMISGRGLGH TGGTLDKLES IPGFNVIQSP EQMQVLLDQA GCCIVGQSEQ LVPADGILYA ARDVTATVDS LPLITASILS KKLVEGLSAL VVDVKFGGAA VFPNQEQARE LAKTLVGVGA SLGLRVAAAL TAMDKPLGRC VGHALEVEEA LLCMDGAGPP DLRDLVTTLG GALLWLSGHA GTQAQGAARV AAALDDGSAL GRFERMLAAQ GVDPGLARAL CSGSPAERRQ LLPRAREQEE LLAPADGTVE LVRALPLALV LHELGAGRSR AGEPLRLGVG AELLVDVGQR LRRGTPWLRV HRDGPALSGP QSRALQEALV LSDRAPFAAP SPFAELVLPP QQ
  • 蛋白标签：
    Tag type will be determined during the manufacturing process.
    The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.
  • 产品提供形式：
    Lyophilized powder
    Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
  • 复溶：
    We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
  • 储存条件：
    Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
  • 保质期：
    The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
    Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
  • 货期：
    Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
    Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
  • 注意事项：
    Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
  • Datasheet ：
    Please contact us to get it.

产品评价

靶点详情

  • 功能：
    May have a role in maintaining the integrity of the blood vessels. Has growth promoting activity on endothelial cells, angiogenic activity in vivo and chemotactic activity on endothelial cells in vitro.; Catalyzes the reversible phosphorolysis of thymidine. The produced molecules are then utilized as carbon and energy sources or in the rescue of pyrimidine bases for nucleotide synthesis.
  • 基因功能参考文献：
    1. Data show that thymidine phosphorylase (TYMP) positive patients had higher systemic immune-inflammation index (SII) score. PMID: 27283904
    2. Investigated the contribution of myeloma-expressed thymidine phosphorylase (TP) to bone lesions. In osteoblast progenitors, TP up-regulated the methylation of RUNX2 and osterix, leading to decreased bone formation. In osteoclast progenitors, TP up-regulated the methylation of IRF8, leading to increased bone resorption. PMID: 27559096
    3. TP(thymidine phosphorylase ) curbed the expression of three proteins-IRF8, RUNX2, and osterix. This downregulation was epigenetically driven: High levels of 2DDR, a product of TP secreted by myeloma cells, activated PI3K/AKT signaling and increased the methyltransferase DNMT3A's expression PMID: 27658717
    4. TP expression is associated with tumor stage, histological grade and thrombocytosis in patients with renal cell carcinoma PMID: 27532673
    5. Studied the role of thymidine phosphorylase (TP) in promoting the invasion and metastasis of hepatocellular carcinoma (HCC). It was shown that TP correlated with MMP2 and MMP9 expression, and that TP mediated the migration of tumor cells through activity of MMPs in HCC cell lines. PMID: 28530649
    6. High VEGF expression was subsequently correlated with a short overall survival rate for patients exhibiting lymph node metastasis (P=0.0128); however, there was no significant difference in overall survival rate regarding the expression levels of TP and CD34 PMID: 26676225
    7. Pretreatment expression of thymidine phosphorylase/HIF-1alpha were found to predict pathologic response and outcomes in clinical stage II/III rectal cancer receiving neoadjuvant chemoradiotherapy. PMID: 26617778
    8. Gastrointestinal cancer patient carriers of the thymidine phosphorylase rs11479 T allele, with a low platelet count, could be at risk of a poor outcome. PMID: 25027354
    9. 4-methylumbelliferone exerts its antitumor effect on ovarian cancer through suppressing thymidine phosphorylase expression. PMID: 25304388
    10. action of thymidine phosphorylase in non-small cell lung cancer: crosstalk with Nrf2 and HO-1 PMID: 24819505
    11. These results demonstrate that the use of AAV to direct TYMP expression in liver is feasible as a potentially safe gene therapy strategy for Mitochondrial neurogastrointestinal encephalomyopathy. PMID: 24448160
    12. thymidine phosphorylase could strongly influence gastric cancer progression via the dual activities of angiogenesis and lymphangiogenesis PMID: 24798152
    13. We suggest that a chip including DPYD, TYMS, TYMP, TK1, and TK2 genes is a potential tool to predict response in LARC following fluoropyrimidine-based CCRT PMID: 24455740
    14. our study shows that IFN-alpha enhanced 5'-DFUR-induced apoptosis in gastric cancer cells by upregulation of TP expression, which is partially regulated by activation of ERK signaling PMID: 24027750
    15. Case Report: thymidine phosphorylase deficiency is a multisystem and ultimately fatal disease. PMID: 20151198
    16. thymidine phosphorylase is more likely expressed by malignant B cells in higher-grade lymphomas PMID: 24617035
    17. the first evidence of the significant impact of the TYMP genotype upon the clinical outcome of patients treated with HLA-identical sibling allo-SCT. PMID: 23813863
    18. The expressions of PD-ECGF and VEGF are higher in the colorectal carcinoma patients with schistosomiasis than in the colorectal carcinoma patients without schistosomiasis. PMID: 24024441
    19. These findings suggest that thymidine phosphorylase plays an important role in angiogenesis, extracellular matrix remodeling and in the prognosis of patients with colorectal cancer PMID: 23160694
    20. Sp1 binding sites in the gliostatin (GLS)promoter are essential for GLS mRNA expression. PMID: 22534375
    21. Thymidine phosphorylase induced STAT3 phosphorylation and increased total amount of unphosphorylated-STAT3 in vascular smooth muscle cells (VSMC), which may play a critical role in inhibiting VSMC proliferation PMID: 22668509
    22. A report of two brothers in whom novel TYMP gene mutations (c.215-13_215delinsGCGTGA; c.1159 2T > A) were associated with different clinical presentations and outcomes. PMID: 22618301
    23. PD-ECGF and VEGF have a synergetic effect in the proliferation of micro-vessels, and they play an important role in the proliferation and involution of hemangioma. PMID: 21837996
    24. The enzymatic activity of thymidine phosphorylase was required for the enhanced expression of IL-8 in human cancer cells. PMID: 22751949
    25. TP gene expression levels in primary CRC tissues and the primary tumor site may be useful predictors of the efficacy of oral UFT/LV chemotherapy. PMID: 22752215
    26. Data indicate that high thymidine phosphorylase (TP) tumor gene expression was associated with obtaining pathological complete response (pCR). PMID: 22610353
    27. Elevated cytoplasmic hnRNP K and TP overexpression are associated with poorer survival in oral squamous cell carcinoma patients PMID: 22321252
    28. High TP/PD-ECGF expression and thrombocytosis can be regarded as valuable tools for predicting overall survival in patients with gastric carcinoma. PMID: 22595739
    29. The expression of PD-ECGF may play an important role in the development and invasion of renal cell carcinoma. PMID: 22340655
    30. In Chinese advanced gastric cancer, Thymidine Phosphorylase positive & beta-tubulin III negative might predict response and prognosis to capecitabine plus paclitaxel chemotherapy. PMID: 21586171
    31. In grade 3 samples of CIN (Cervical Intraepithalial Neoplasia), expression of TP (separately or in combination with Ki-67 antigen) appeared to be related to imminent progression to invasive cancer. TP levels were shown to correlate directly with CIN grade PMID: 21809665
    32. Thymidine phosphorylase involvement in the enhancement of IgE production PMID: 21252618
    33. A significant correlation was noticed between HIF-1alpha and vascular endothelial growth factor expression while the expression levels of thymidine phosphorylase, cyclooxygenase and microvessel density were significantly higher in high HIF-2alpha tumors. PMID: 21362301
    34. Both TP and MK are important for angiogenesis in laryngeal squamous cell carcinoma. The expression of TP, MK and CD105 were all correlated with T-stage and lymph node metastasis. PMID: 18476626
    35. Thymidine phosphorylase did not increase the tumour invasion, but stimulated the migration and invasion of endothelial cells by two different mechanisms. PMID: 21386840
    36. transition state for the arsenolytic reaction PMID: 21222488
    37. altered function of hnRNP H1/H2 in tumor cells is a novel determinant of aberrant thymidine phosphorylase splicing thereby resulting in acquired chemoresistance to TP-activated fluoropyrimidine anticancer drugs. PMID: 21068389
    38. thymidine phosphorylase is expressed differently in gallbladder and bile duct cancer PMID: 20955617
    39. MNGIE is caused by mutations in the gene encoding thymidine phosphorylase, which lead to absolute or nearly complete loss of its catalytic activity, producing systemic accumulations of its substrates, thymidine and deoxyuridine. PMID: 20585803
    40. thymidine phosphorylase has a role in the induction of early growth response protein-1 and thrombospondin-1 by 5-fluorouracil in human cancer carcinoma cells PMID: 20372793
    41. Thymidine phosphorylase-derived sugars from overexpressing tumor cells accumulate in the cytoskeleton and to some extent in the cell membrane, enabling endothelial cells to migrate and invade towards tumor sites. PMID: 20488166
    42. TP may have a role in angiogenesis-dependent growth and migration of cholangiocarcinoma and in response to 5-fluorouracil chemotherapy PMID: 20355241
    43. Increased thymidine phosphorylase expression is associated with bladder cancers. PMID: 20112501
    44. dThdPase activity in gastric cancer cells was found to be correlated with venous invasion, supporting previous findings that it plays a role in tumor angiogenesis PMID: 9857235
    45. induction in tumoral stroma, but not in tumor cells, may promote angiogenesis in adenocarcinoma of the lung; may be important prognostic factor in adenocarcinoma of the lung PMID: 11920479
    46. inhibits the intracellular apoptotic signal transduction in the Fas-induced apoptotic pathway PMID: 11925935
    47. COX-2 is upregulated in endometrial cancer and facilitates tumor growth via angiogenesis produced in associated with VEGF and TP. PMID: 11957147
    48. thymidine phosphorylase is a major angiogenic factor in prostate carcinoma and its upregulation is likely to occur in the context of host immune response PMID: 11986782
    49. summary of research advances concerning thymidine phosphorylase in breast carcinoma (review) PMID: 12174926
    50. Biochemical defects of thymidine phosphorylase (hPD-ECGF) and a pathogenic G-to-A transition mutation at nucleotide 435 in the hPD-ECGF gene have been identified in two affected siblings with mitochondrial neurogastrointestinal encephalomyopathy (MNGIE). PMID: 12177387

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  • 相关疾病：
    Mitochondrial DNA depletion syndrome 1, MNGIE type (MTDPS1)
  • 蛋白家族：
    Thymidine/pyrimidine-nucleoside phosphorylase family
  • 数据库链接：

    HGNC: 3148

    OMIM: 131222

    KEGG: hsa:1890

    STRING: 9606.ENSP00000252029

    UniGene: Hs.180903