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  4. Recombinant Human Spindle and kinetochore-associated protein 2 (SKA2)

Recombinant Human Spindle and kinetochore-associated protein 2 (SKA2)

  • 中文名称：
    人SKA2重组蛋白
  • 货号：
    CSB-YP823890HU
  • 规格：
  • 来源：
    Yeast
  • 其他：
  • 中文名称：
    人SKA2重组蛋白
  • 货号：
    CSB-EP823890HU
  • 规格：
  • 来源：
    E.coli
  • 其他：
  • 中文名称：
    人SKA2重组蛋白
  • 货号：
    CSB-EP823890HU-B
  • 规格：
  • 来源：
    E.coli
  • 共轭：
    Avi-tag Biotinylated

    E. coli biotin ligase (BirA) is highly specific in covalently attaching biotin to the 15 amino acid AviTag peptide. This recombinant protein was biotinylated in vivo by AviTag-BirA technology, which method is BriA catalyzes amide linkage between the biotin and the specific lysine of the AviTag.

  • 其他：
  • 中文名称：
    人SKA2重组蛋白
  • 货号：
    CSB-BP823890HU
  • 规格：
  • 来源：
    Baculovirus
  • 其他：
  • 中文名称：
    人SKA2重组蛋白
  • 货号：
    CSB-MP823890HU
  • 规格：
  • 来源：
    Mammalian cell
  • 其他：

产品详情

  • 纯度：
    >85% (SDS-PAGE)
  • 基因名：
    SKA2
  • Uniprot No.：
  • 别名：
    FAM33A; Family with sequence similarity 33; member A; FLJ12758; MGC110975; Protein FAM33A; SKA 2; SKA2; SKA2_HUMAN; Spindle and kinetochore associated complex subunit 2; Spindle and kinetochore associated protein 2; Spindle and kinetochore-associated protein 2; Spindle and KT (kinetochore) associated 2; Spindle and KT associated 2
  • 种属：
    Homo sapiens (Human)
  • 蛋白长度：
    full length protein
  • 表达区域：
    1-121
  • 氨基酸序列
    MEAEVDKLEL MFQKAESDLD YIQYRLEYEI KTNHPDSASE KNPVTLLKEL SVIKSRYQTL YARFKPVAVE QKESKSRICA TVKKTMNMIQ KLQKQTDLEL SPLTKEEKTA AEQFKFHMPD L
  • 蛋白标签：
    Tag type will be determined during the manufacturing process.
    The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.
  • 产品提供形式：
    Lyophilized powder
    Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
  • 复溶：
    We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
  • 储存条件：
    Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
  • 保质期：
    The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
    Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
  • 货期：
    Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
    Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
  • 注意事项：
    Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
  • Datasheet ：
    Please contact us to get it.

产品评价

靶点详情

  • 功能：
    Component of the SKA1 complex, a microtubule-binding subcomplex of the outer kinetochore that is essential for proper chromosome segregation. Required for timely anaphase onset during mitosis, when chromosomes undergo bipolar attachment on spindle microtubules leading to silencing of the spindle checkpoint. The SKA1 complex is a direct component of the kinetochore-microtubule interface and directly associates with microtubules as oligomeric assemblies. The complex facilitates the processive movement of microspheres along a microtubule in a depolymerization-coupled manner. In the complex, it is required for SKA1 localization. Affinity for microtubules is synergistically enhanced in the presence of the ndc-80 complex and may allow the ndc-80 complex to track depolymerizing microtubules.
  • 基因功能参考文献：
    1. decreased neuronal cell SKA complex genes' expression levels affect neuronal cell viability and neurite development PMID: 29268205
    2. study shows that decreased gene and protein expression of SKA2 observed in the prefrontal cortex of suicide victims is specific to suicide, which was not observed in the brain of nonsuicidal patients. It also indicates reduced SKA2 expression in suicide is independent of psychiatric diagnosis, since it is observed in all diagnostic groups studied. PMID: 26902949
    3. Possible association between SKA2 expression in prefrontal cortex and MDD. PMID: 27030168
    4. Data show that HOTAIR might act as an endogenous 'sponge' of miR-141, thereby regulating the derepression of SKA2. PMID: 27121316
    5. The study adds evidence that CREB, a tumor oncogene, promotes renal cell carcinoma proliferation. It probably achieves this by increasing SKA2 expression PMID: 26824422
    6. Kinetochores mature through Ska1/ska2/Ska3 complex recruitment and this is required for improved load-bearing capacity and silencing of the spindle assembly checkpoint. PMID: 28495837
    7. p53 negatively regulates the expression of the PRR11-SKA2 bidirectional transcription unit through NF-Y, suggesting that the inability to repress the PRR11-SKA2 bidirectional transcription unit after loss of p53 might contribute to tumorigenesis. PMID: 28257042
    8. these data establish the importance of SKA2 for cortisol stress responsivity and the development of post-traumatic stress disorder and provide further evidence that SKA2 is a promising biomarker for stress-related disorders including PTSD. PMID: 26361058
    9. Results suggest that DNA methylation(adj) of SKA2 in blood indexes stress-related psychiatric phenotypes and neurobiology, pointing to its potential value as a biomarker of stress exposure and susceptibility. PMID: 26324104
    10. DNA methylation of the SKA2 gene has been implicated as a biomarker of suicide risk and posttraumatic stress disorder. PMID: 27038412
    11. SKA2 is hypothesized to reduce the ability to suppress cortisol following stress. PMID: 26305478
    12. PRR11-SKA2 bidirectional transcription unit, which is a novel direct target of NF-Y, is essential for the accelerated proliferation and motility of lung cancer cells PMID: 26162986
    13. SKA2 significantly interacted with anxiety and stress to explain about 80% of suicidal behavior and progression from suicidal ideation to suicide attempt.SKA2 is a novel genetic and epigenetic marker involved in the etiology of suicide, suicidal behaviors PMID: 25073599
    14. The structure of the Ska core complex is a W-shaped dimer of coiled coils, formed by intertwined interactions between Ska1, Ska2, and Ska3. PMID: 22483620
    15. Data suggest that Aurora B phosphorylation antagonizes the interaction between the Ska1-3 complex and the KMN network, thereby controlling Ska recruitment to KTs and stabilization of KT-MT attachments. PMID: 22371557
    16. blocking of miR-301 in A549 cells leads to a decrease in the expression of the host gene, ska2. PMID: 20470754
    17. These data suggest that the Ska1/Ska2 complex plays a critical role in the maintenance of the metaphase plate and/or spindle checkpoint silencing. PMID: 17093495
    18. Study discovered that FAM33A is protein partners for GRs and is involved in cell proliferation and GC signalling. PMID: 18583474

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  • 亚细胞定位：
    Cytoplasm, cytoskeleton, spindle. Chromosome, centromere, kinetochore.
  • 蛋白家族：
    SKA2 family
  • 数据库链接：

    HGNC: 28006

    OMIM: 616674

    KEGG: hsa:348235

    STRING: 9606.ENSP00000333433

    UniGene: Hs.463607