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  4. Recombinant Human Serum paraoxonase/arylesterase 1 (PON1)

Recombinant Human Serum paraoxonase/arylesterase 1 (PON1)

In Stock
  • 货号：
    CSB-EP018369HU
  • 规格：
    ￥1344
  • 图片：
    • (Tris-Glycine gel) Discontinuous SDS-PAGE (reduced) with 5% enrichment gel and 15% separation gel.
  • 其他：

产品详情

  • 纯度：
    Greater than 90% as determined by SDS-PAGE.
  • 基因名：
    PON1
  • Uniprot No.：
  • 别名：
    A esterase 1; A-esterase 1; Aromatic esterase 1; Arylesterase 1; Arylesterase B type; ESA; Esterase A; K 45; K-45; MVCD5; Paraoxonase 1; Paraoxonase; Paraoxonase B type; Paraoxonase, plasma; Paraoxonase1; PON 1; PON; PON1; PON1_HUMAN; Serum aryldiakylphosphatase; Serum aryldialkylphosphatase 1; Serum paraoxonase/arylesterase 1
  • 种属：
    Homo sapiens (Human)
  • 蛋白长度：
    Full Length of Mature Protein
  • 来源：
    E.coli
  • 分子量：
    66.6kDa
  • 表达区域：
    2-355aa
  • 氨基酸序列
    AKLIALTLLGMGLALFRNHQSSYQTRLNALREVQPVELPNCNLVKGIETGSEDLEILPNGLAFISSGLKYPGIKSFNPNSPGKILLMDLNEEDPTVLELGITGSKFDVSSFNPHGISTFTDEDNAMYLLVVNHPDAKSTVELFKFQEEEKSLLHLKTIRHKLLPNLNDIVAVGPEHFYGTNDHYFLDPYLQSWEMYLGLAWSYVVYYSPSEVRVVAEGFDFANGINISPDGKYVYIAELLAHKIHVYEKHANWTLTPLKSLDFNTLVDNISVDPETGDLWVGCHPNGMKIFFYDSENPPASEVLRIQNILTEEPKVTQVYAENGTVLQGSTVASVYKGKLLIGTVFHKALYCEL
    Note: The complete sequence including tag sequence, target protein sequence and linker sequence could be provided upon request.
  • 蛋白标签：
    N-terminal GST-tagged
  • 产品提供形式：
    Liquid or Lyophilized powder
    Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
  • 缓冲液：
    Tris-based buffer,50% glycerol
  • 储存条件：
    Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
  • 保质期：
    The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
    Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
  • 货期：
    3-7 business days
  • 注意事项：
    Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
  • 产品描述：

    The expression of recombinant human PON1 protein includes the construction of the expression vector containing the recombinant DNA and the transformation of the expression vector into the E.coli, which provides a variety of macromolecules and components required for transcription and translation. The recombinant DNA was formed by fusing the N-terminal GST tag sequence to the designated sequence encoding the 2-355aa of the human PON1 protein. This N-terminal 6xHis-tagged recombinant human PON1 protein is also characterized by high purity, >90%. Under SDS-PAGE condition, this recombinant PON1 protein migrated to the band of about 55 kDa molecular weight.

    Commonly, the expression of PON1 has been mainly found in liver. PON1, as the high-density lipoprotein (HDL)-associated serum enzyme, it exhibits a broad substrate specificity. Low PON1 activity found in children may increase their susceptibility to organophosphates. PON1 is responsible for protecting against exposure to some organophosphorus (OP) pesticides as well as vascular disease. Many findings suggested that PON1 participates in the activities of pesticide sensitivity, cardiovascular disease, and drug metabolism. Meanwhile, PON1 as the antioxidant enzyme, it has been recently confirmed as potential prognostic predictor of acute ischemic stroke. Certain studies indicated that Decreased PON1 activity and PON1 polymorphisms are associated with chronic kidney disease in type 2 diabetes mellitus. More important, researchers recently implied the association of PON1 polymorphisms activity and colorectal cancer predisposition.

  • Datasheet & COA：
    Please contact us to get it.

产品评价

靶点详情

  • 功能：
    Hydrolyzes the toxic metabolites of a variety of organophosphorus insecticides. Capable of hydrolyzing a broad spectrum of organophosphate substrates and lactones, and a number of aromatic carboxylic acid esters. Mediates an enzymatic protection of low density lipoproteins against oxidative modification and the consequent series of events leading to atheroma formation.
  • 基因功能参考文献：
    1. serum levels decrease in states of high oxidative stress like metabolic syndrome, obesity, uncontrolled diabetes, and dyslipidemia PMID: 29843993
    2. CD was found to be associated with the decrease in the levels of PON-1, which correlates well with activity of the disease and reflects the intensification of inflammation, as well as intensified lipid peroxidation. High sensitivity and specificity of PON-1 determines its selection as a good screening test for CD severity. PMID: 30314292
    3. The analysis of the effect of the individual SNPs(PON1, IL-6, ITGB3, and ALDH2 ) and GRS groups on different lipid profile parameters revealed no significant association of any of the tested SNPs with any lipid parameter, however, the GRS groups showed marginally significant for TC and highly significant association for TG, LDL-c and HDL-c PMID: 30261890
    4. It findings provide genetic evidence that the PON1-L55M variant may be a factor contributing to the glycemic control in type 2 diabetes. PMID: 29782842
    5. Low serum paraoxonase 1 activity is associated with the development of type 2 diabetes mellitus. PMID: 29156090
    6. T allele and TT genotype are associated with COPD, and the PON1-108C>T polymorphism could be a potential predictor of the disease. PMID: 29858231
    7. study showed that altered HDL subclasses distribution, changed PON1 activities on different HDL subclasses as well as diminished anti-oxidative protection could be important factors in atherosclerosis development in CKD and ESRD patients PMID: 30130521
    8. Data suggest that patients with ARF (acute renal failure) caused by septic shock have low serum PON1 activities, cholesterol, and HDL-cholesterol; high serum PON1 activities are associated with severity of septic shock; extra-renal depuration techniques produce further increases in serum PON1 activities related to duration of stay in ICU and increases in serum urea levels. This study was conducted in Spain. PMID: 30165052
    9. Studied association of genetic polymorphisms of paraoxanase 1(PON1) in Turkish patients with pulmonary embolism (PE). Results suggest that there is no relationship between both PON 1 polymorphisms and PE in the Turkish population. PMID: 29682786
    10. Low PON1 expression is associated with breast cancer. PMID: 29970690
    11. serum concentrations of trace elements and their relationships with paraoxonase-1 in morbidly obese women PMID: 29773198
    12. Low PON1 expression is associated with Atherosclerosis. PMID: 29254890
    13. The aim of this study was to investigate whether obesity is associated with PON1 activity and whether this association is influenced by oxidative stress, inflammation and HDL cholesterol (HDL-C) concentration. PMID: 29168398
    14. Our results showed that ApoE epsilon4 and PON1-55M alleles act synergistically to increase the risk of systemic lupus erythematosus by 1.47 times PMID: 29273831
    15. Low PON1 expression is associated with endometrial cancer. PMID: 30178714
    16. The rs3735590 polymorphism of PON1 acts as a prognostic biomarker in COPD patients treated by CABG. PMID: 29953969
    17. Results showed significantly decreased levels of PON1 in patients with chronic liver diseases (controls 185 +/- 14 U/l, NAFLD 160 +/- 15 U/l, chronic hepatitis 99 +/- 18 U/l, cirrhosis (52 +/- 11 U/l). PMID: 29322801
    18. rs662_A allele carriers may benefit from intake of vegetables and thus be more effectively protected from ischemic stroke. Carriers of the G allele may still remain at greater risk for ischemic stroke even when they consume a high level of vegetables. PMID: 29215590
    19. Impaired anti-oxidant and anti-atherogenic HDL properties associated with weakened PON1 function and lipid peroxidation may contribute to the development of atherosclerosis-related diseases in T2DM. PMID: 29626583
    20. the Q192R polymorphism in the PON1 gene is associated with familial hypercholesterolemia (FH) in the Saudi population; the R allele, QR, and dominant model genotypes were associated with FH PMID: 29229890
    21. activity of PON1 in the control group was significantly higher in comparison with diabetic patients PMID: 28866115
    22. PON1-L55M T alleles are associated with dementia risk. PMID: 28657841
    23. PON1 Q192R polymorphism has a weak association with coronary heart disease risk in Chinese PMID: 29952962
    24. rs854560 polymorphism may modulate the risk of coronary artery disease in response to cigarette smoking in Polish population. PMID: 29118461
    25. PLA2G7 and PON1 are overexpressed in prostatic neoplasm patients and can be detected early in blood. PMID: 29050675
    26. PON1 as an important candidate gene influencing genetic pathophysiology of polycystic ovarian syndrome. PMID: 29604466
    27. It demonstrates that the impact of Y71 substitutions on PON1's lactonase activity is minimal, whereas the kcat for the paraoxonase activity is negatively perturbed by up to 100-fold, suggesting greater mutational robustness of the native activity. PMID: 28026940
    28. genetic association studies in population of women in Greece: Data suggest than a genetic polymorphism in PON1 (Q192R) is associated with gestational diabetes in the population studied. Transcription of the PON1 gene does not appear to be impaired in leukocytes from women with gestational diabetes. PMID: 28347194
    29. The patients with CT OR TT genotype at loci rs3735590 had a lower risk of CAVS than patients with the CC genotype. PMID: 29462797
    30. HDL-C, but not its anti-oxidant constituent, PON-1, is inversely, continuously and independently associated with future risk of hypertension. PMID: 28667918
    31. In donor retina from patients with diabetes, all three PON1, PON2 and PON3 were expressed, and there was a significant increase in PON3 expression compared to control. This might be the reason for the increased thiolactonase activity observed in diabetic retina compared to control PMID: 28862184
    32. Serum PON1 levels showed that oxidative stress was severe in otosclerosis. PMID: 27387094
    33. PON1 L55M genetic polymorphisms may be associated with the risk of breast cancer and could potentially serve as useful genetic markers for tumor prognosis in some populations of Chinese women. PMID: 28445984
    34. Paraoxonase-1 (PON1) induces metastatic potential and apoptosis escape via its antioxidative function in lung cancer cells PMID: 28467805
    35. This study is the first study to investigate serum PON1 enzyme activity in patients with cutaneous anthrax. We concluded that oxidative stress was increased whereas serum PON1 activity was decreased in patients with cutaneous anthrax. These results indicate that lower PON1 activity is associated with an oxidant-antioxidant imbalance. PMID: 27461010
    36. The L55M polymorphism associated with systemic lupus erythematosus and anti-phospholipid syndrome in a population from Cairo, Egypt, while the Q192R polymorphism played no role in disease susceptibility. PMID: 28185016
    37. PON1 (Q192R and L55M) polymorphisms may play a crucial role in pathogenesis and susceptibility of insulin resistance thus leads to the development of type 2 diabetes in South Indian population. PMID: 29409844
    38. PON1 protein can be detected in plasma and resides in the high-density lipoprotein fraction and protects against Oxidative stress by hydrolyzing certain oxidized lipids in lipoproteins, macrophages and Atherosclerotic lesions. PMID: 29308836
    39. Our study provides preliminary support for the involvement of organophosphate pesticides and PON1 in Parkinson's disease-related motor, cognitive, or depressive symptom progression. PMID: 28689109
    40. The distribution of genotype frequencies in the assessed women (PON1 Q192R polymorphism) was QQ = 20%, QR = 48% and RR = 32%. Significantly higher serum FABP4 levels were found in women with genotype QR/RR (20.6 +/- 2.20 ng/mL), when compared with the levels found in the QQ group (12.8 +/- 1.70 ng/mL) (p = .004). PMID: 27712128
    41. rare genetic variation in PON1 was associated with ischemic stroke, with stronger associations identified in those of AA. Increased focus on PON1 enzyme function and its role in cerebrovascular disease is warranted. PMID: 24711634
    42. PON1 arylesterase activity correlated negatively with sCD40L, ADMA, and sICAM-1 levels in overweight patients with newly diagnosed untreated hyperlipidaemia. PMID: 28602123
    43. 5-amino-2-methylbenzenesulfonamide showed competitive inhibition and so molecular docking studies were performed for this compound in order to assess the probable binding mechanism into the active site of hPON1. PMID: 28665493
    44. The results of various studies in different populations indicate that some SNPs of the PON1 gene are associated with stroke. PMID: 28779954
    45. Genotype RR of PON1 Q192R is an independent risk factor predicting re-stenting in Chinese acute coronary syndrome patients after coronary stenting PMID: 27450784
    46. Clinical observations focusing on gene polymorphisms of PON indicate that three different genotypes of polymorphism PON1Q192R have unequal degrees atheroprotective properties. PMID: 29215249
    47. the measurement of serum PON1 concentration post-radiotherapy (RT) could be an efficient prognostic biomarker, and may be used as an index of the efficacy of the RT. PMID: 29176871
    48. Patients with the Q allele of the PON1 Q192R polymorphism who were treated with statins exhibited improvement in glucose metabolism, especially in insulin secretion, suggesting the importance of genotyping PON1 Q192R to identify those who could benefit from statin therapy. PMID: 29233102
    49. PON1 and CYP2C19 polymorphisms were associated with lower clopidogrel responsiveness in this sample. PMID: 28076455
    50. Our study showed that although lipoic acid up-regulates PON3 but down-regulates PON1 mRNA expression, it increases both PON1 and PON3 protein levels and arylesterase activity in HepG2 cells. We can report that lipoic acid may be useful for preventing atherosclerosis at therapeutic doses. PMID: 28653653

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  • 相关疾病：
    Microvascular complications of diabetes 5 (MVCD5)
  • 亚细胞定位：
    Secreted, extracellular space.
  • 蛋白家族：
    Paraoxonase family
  • 组织特异性：
    Plasma, associated with HDL (at protein level). Expressed in liver, but not in heart, brain, placenta, lung, skeletal muscle, kidney or pancreas.
  • 数据库链接：

    HGNC: 9204

    OMIM: 168820

    KEGG: hsa:5444

    STRING: 9606.ENSP00000222381

    UniGene: Hs.370995