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  4. Recombinant Human Semaphorin-3F (SEMA3F)

Recombinant Human Semaphorin-3F (SEMA3F)

  • 货号：
    CSB-YP622647HU
  • 规格：
  • 来源：
    Yeast
  • 其他：
  • 货号：
    CSB-EP622647HU
  • 规格：
  • 来源：
    E.coli
  • 其他：
  • 货号：
    CSB-EP622647HU-B
  • 规格：
  • 来源：
    E.coli
  • 共轭：
    Avi-tag Biotinylated

    E. coli biotin ligase (BirA) is highly specific in covalently attaching biotin to the 15 amino acid AviTag peptide. This recombinant protein was biotinylated in vivo by AviTag-BirA technology, which method is BriA catalyzes amide linkage between the biotin and the specific lysine of the AviTag.

  • 其他：
  • 货号：
    CSB-BP622647HU
  • 规格：
  • 来源：
    Baculovirus
  • 其他：

产品详情

  • 纯度：
    >85% (SDS-PAGE)
  • 基因名：
    SEMA3F
  • Uniprot No.：
  • 别名：
    SEM3F_HUMAN; sema domain immunoglobulin domain Ig short basic domain secreted 3F; sema domain immunoglobulin domain Ig short basic domain secreted semaphorin 3F; Sema III/F; Sema IV; SEMA-IV; SEMA3F; SEMA4; SemaIII/F; SemaIV; SEMAK; Semaphorin IV; Semaphorin-3F; Semaphorin3F; SemaphorinIV
  • 种属：
    Homo sapiens (Human)
  • 蛋白长度：
    Full Length of Mature Protein
  • 表达区域：
    19-785
  • 氨基酸序列
    SF PTQDHLPATP RVRLSFKELK ATGTAHFFNF LLNTTDYRIL LKDEDHDRMY VGSKDYVLSL DLHDINREPL IIHWAASPQR IEECVLSGKD VNGECGNFVR LIQPWNRTHL YVCGTGAYNP MCTYVNRGRR AQATPWTQTQ AVRGRGSRAT DGALRPMPTA PRQDYIFYLE PERLESGKGK CPYDPKLDTA SALINEELYA GVYIDFMGTD AAIFRTLGKQ TAMRTDQYNS RWLNDPSFIH AELIPDSAER NDDKLYFFFR ERSAEAPQSP AVYARIGRIC LNDDGGHCCL VNKWSTFLKA RLVCSVPGED GIETHFDELQ DVFVQQTQDV RNPVIYAVFT SSGSVFRGSA VCVYSMADIR MVFNGPFAHK EGPNYQWMPF SGKMPYPRPG TCPGGTFTPS MKSTKDYPDE VINFMRSHPL MYQAVYPLQR RPLVVRTGAP YRLTTIAVDQ VDAADGRYEV LFLGTDRGTV QKVIVLPKDD QELEELMLEE VEVFKDPAPV KTMTISSKRQ QLYVASAVGV THLSLHRCQA YGAACADCCL ARDPYCAWDG QACSRYTASS KRRSRRQDVR HGNPIRQCRG FNSNANKNAV ESVQYGVAGS AAFLECQPRS PQATVKWLFQ RDPGDRRREI RAEDRFLRTE QGLLLRALQL SDRGLYSCTA TENNFKHVVT RVQLHVLGRD AVHAALFPPL SMSAPPPPGA GPPTPPYQEL AQLLAQPEVG LIHQYCQGYW RHVPPSPREA PGAPRSPEPQ DQKKPRNRRH HPPDT
  • 蛋白标签：
    Tag type will be determined during the manufacturing process.
    The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.
  • 产品提供形式：
    Lyophilized powder
    Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
  • 复溶：
    We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
  • 储存条件：
    Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
  • 保质期：
    The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
    Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
  • 货期：
    Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
    Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
  • 注意事项：
    Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
  • Datasheet ：
    Please contact us to get it.

产品评价

靶点详情

  • 功能：
    May play a role in cell motility and cell adhesion.
  • 基因功能参考文献：
    1. SEMA3F plays a role as a tumor suppressor in Oral squamous cell carcinoma cell proliferation, migration and invasion. PMID: 29299034
    2. in situ hybridization analysis revealed that Sema 3C and Sema 3F are expressed at the RNA level in the endometriosis affected peritoneum PMID: 27558236
    3. Semaphorin 3F placenta tissue expression was significantly reduced in preeclampsia. In addition, semaphorin 3F level at delivery was significantly lower in serum, amniotic fluid and venous umbilical blood of preeclamptic patients compared with normal pregnant women. PMID: 28350837
    4. There is a positive association between the expression of AKAP12 and Semaphorin 3F in prostate cancer, suggesting that the activation of Semaphorin 3F by AKAP12 may be involved in prostate cancer progression and metastasis. PMID: 28698137
    5. Study demonstrates an anti-tumoral role of SEMA3F in ileal NETs. We thus suggest that SEMA3F and/or its cellular signaling pathway could represent a target for ileal NET therapy. PMID: 26447612
    6. SEMA3F was downregulated in colorectal cancer tissues as compared to matched adjacent non-tumor tissues PMID: 26722466
    7. A new SEMA3F transcript is expressed in all breast cell lines and breast cancer biopsies, and is translated into a new semaphorin 3F isoform. PMID: 26784191
    8. SEMA3F-NRP2 interactions inhibit intracellular PI-3K activity, mTORC2-dependent signaling, RhoA activity and cytoskeletal stress fiber formation. PMID: 26156437
    9. Data indicate that semaphorin 3F (SEMA3F) and its receptor neuropilin-2 (NRP2) are expressed in the thymus. PMID: 25068647
    10. SEMA3F functions as a suppressor of colorectal cancer metastasis by down-regulating the ASCL2-CXCR4 signaling axis. PMID: 25866254
    11. SEMA3F may represent an antilymphangiogenic metastasis suppressor gene widely lost during cancer progression, hence serving as a prognostic biomarker and an attractive target for therapeutic intervention to halt metastasis. PMID: 25952650
    12. Infantile hemangioma-derived stem cells and endothelial cells are inhibited by SEMA3E and SEMA3F. PMID: 26086095
    13. Data suggest that SEMA3F C-terminal domain exhibits high-affinity binding of neuropilin-1 (NRP1; thus inhibiting binding of vascular endothelial growth factor A to NRP1); this interaction may be involved in anti-angiogenic activity of SEMA3F. PMID: 24079887
    14. A functional role for Semaphorin 3F in the outer retina where it acts as a vasorepulsive cue to maintain physiologic avascularity. PMID: 23603393
    15. This study demonstrated a marked loss of noradrenergic and sensory nerve fibers in polyp mucosa, which was associated with a strong increase of semaphorin 3F and 3A. PMID: 22093159
    16. It was concluded that hypoxia regulates VEGF and SE MA3F activities through transcriptional repression of their common receptor NRP2, providing a novel mechanism by which hypoxia induces tumor angiogenesis, growth and metastasis. PMID: 21610314
    17. Endogenous SEMA3F acts as a suppressor of the growth and metastasis of human colorectal cancer cells. PMID: 21349996
    18. SEMA3F, CLEC16A, LAMA3, and PCSK2 variants have roles in myocardial infarction in Japanese individuals PMID: 20036365
    19. semaphorin-3B and semaphorin-3F have roles in ovarian cancer PMID: 20124444
    20. Transient SEMA-3F gene transfection may inhibit the proliferation of Tca8113 cells. PMID: 18476556
    21. Metastatic tumor cells overexpress c-myc, leading to upregulation of Id2 expression; the aberrantly elevated amount of Id2 represses SEMA3F expression and, as a consequence, enhances the ability of tumor cells to migrate and invade. PMID: 20388805
    22. SEMA3F and VEGF have antagonistic actions affecting motility in primary tumor cell PMID: 12659673
    23. SEMA3F is a potent metastasis inhibitor that targets both tumor and stromal cells PMID: 15520858
    24. SEMA3F suppresses lung neoplasm progression in an experimental model PMID: 15967098
    25. Results suggest that p53 negatively regulates tumor vessel formation and cell growth via the SEMA3F-NRP2 pathway. PMID: 17308083
    26. combinations of sema3A and sema3F may be able to inhibit tumor angiogenesis more effectively than single semaphorins. PMID: 17569671
    27. Semaphorin 3F mRNA forms a G quartet-containing structure, which is recognized with high affinity and specificity by the RGG box domain of the fragile X mental retardation protein. PMID: 17693432
    28. ABL2/ARG is a novel mediator of SEMA3F-induced RhoA inactivation and collapsing activity. PMID: 18660502
    29. SEMA3F loss was associated with changes in cell signaling: increased phospho-AKT in normoxia and hypoxia-induced factor 1alpha protein. Exogenous addition of SEMA3F could modulate ZEB-1-induced angiogenesis in a chorioallantoic membrane assay. PMID: 19177200
    30. Semaphorin3F reverses Multicellular resistance by regulating alpha(v)beta3 PMID: 19657188
    31. These data indicate that polymorphisms in SEMA3F are associated with prostate cancer risk and poor prognosis in Hispanic and nonHispanic white men PMID: 19683737
    32. Soluble neuropilin-2Fc did not inhibit repulsion but increased the repellent effect of semaphorin 3F. PMID: 19790074
    33. Sema3F inhibits tumor development from MDA-MB-435 and MDA-MB-231 and but not MCF-7 or MDA-MB-68 cancer cells. It inhibits tumor angiogenesis in all of the formed tumors. The inhibition is correlated with the expression of neuropilins of the tumors cells. PMID: 18818766

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  • 亚细胞定位：
    Secreted.
  • 蛋白家族：
    Semaphorin family
  • 组织特异性：
    Expressed abundantly but differentially in a variety of neural and nonneural tissues. There is high expression in mammary gland, kidney, fetal brain, and lung and lower expression in heart and liver.
  • 数据库链接：

    HGNC: 10728

    OMIM: 601124

    KEGG: hsa:6405

    STRING: 9606.ENSP00000002829

    UniGene: Hs.32981