Your Good Partner in Biology Research

  1. 首页
  2. 蛋白
  3. 重组蛋白
  4. Recombinant Human Riboflavin transporter 2 (RFT2), partial

Recombinant Human Riboflavin transporter 2 (RFT2), partial

  • 货号：
    CSB-YP873618HU
  • 规格：
  • 来源：
    Yeast
  • 其他：
  • 货号：
    CSB-EP873618HU
  • 规格：
  • 来源：
    E.coli
  • 其他：
  • 货号：
    CSB-EP873618HU-B
  • 规格：
  • 来源：
    E.coli
  • 共轭：
    Avi-tag Biotinylated

    E. coli biotin ligase (BirA) is highly specific in covalently attaching biotin to the 15 amino acid AviTag peptide. This recombinant protein was biotinylated in vivo by AviTag-BirA technology, which method is BriA catalyzes amide linkage between the biotin and the specific lysine of the AviTag.

  • 其他：
  • 货号：
    CSB-BP873618HU
  • 规格：
  • 来源：
    Baculovirus
  • 其他：
  • 货号：
    CSB-MP873618HU
  • 规格：
  • 来源：
    Mammalian cell
  • 其他：

产品详情

  • 纯度：
    >85% (SDS-PAGE)
  • 基因名：
    SLC52A3
  • Uniprot No.：
  • 别名：
    bA371L19.1; BVVLS; BVVLS1; C20orf54; C20orf54provided by HGNC; Chromosome 20 open reading frame 54; hRFT2; member 3; MGC10698; RFT2; RFVT3; Riboflavin transporter 2; riboflavin transporter; riboflavin transporter, member 3; S52A3_HUMAN; Slc52a3; solute carrier family 52 (riboflavin transporter), member 3; Solute carrier family 52; solute carrier family 52, riboflavin transporter, member 3
  • 种属：
    Homo sapiens (Human)
  • 蛋白长度：
    Partial
  • 蛋白标签：
    Tag type will be determined during the manufacturing process.
    The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.
  • 产品提供形式：
    Lyophilized powder
    Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
  • 复溶：
    We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
  • 储存条件：
    Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
  • 保质期：
    The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
    Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
  • 货期：
    Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
    Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
  • 注意事项：
    Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
  • Datasheet ：
    Please contact us to get it.

产品评价

靶点详情

  • 功能：
    Plasma membrane transporter mediating the uptake by cells of the water soluble vitamin B2/riboflavin that plays a key role in biochemical oxidation-reduction reactions of the carbohydrate, lipid, and amino acid metabolism. Humans are unable to synthesize vitamin B2/riboflavin and must obtain it via intestinal absorption.
  • 基因功能参考文献：
    1. The riboflavin transporter-3 (SLC52A3) 5'-flanking regions contain NF-kappaB p65/Rel-B-binding sites, which are crucial for mediating SLC52A3 transcriptional activity in esophageal squamous cell carcinoma (ESCC) cells. PMID: 29428966
    2. RFVT3 gene and protein expression levels were higher in DLD-1 and HT-29 compared to Caco2 cells. In tumor tissues of patients with CRC, RFVT3 gene expression levels were increased, while protein expression was reduced, with a small reduction in riboflavin amount. PMID: 29715086
    3. our knowledge, this is the first report to indicate that Rfvt3 contributes to placental riboflavin transport, and that disruption of Slc52a3 gene caused neonatal mortality with hyperlipidemia and hypoglycemia owing to riboflavin deficiency. PMID: 27272163
    4. This study found that RFT2 was overexpressed in glioma samples compared with normal brain tissue. PMID: 27584688
    5. RFVT3 is a target for posttranscriptional regulation by miR-423-5p in intestinal epithelial cells, and this regulation has functional consequences on intestinal riboflavin (RF) uptake process. PMID: 28912250
    6. Fourteen mutations in SLC52a3 were associated with Brown-Vialetto-Van Laere syndrome. PMID: 29053833
    7. SLC52A3 rs13042395 C>T polymorphism was associated with decreased cancer risk in the normal body mass index group, whereas no association was present in obesity group. PMID: 27600099
    8. Single nucleotide polymorphism rs13042395 in the SLC52A3 TT genotype carriers were likely to have reduced lymph node metastasis and longer relapse-free survival time. PMID: 27472962
    9. This study also identified a number of residues in the hRFVT-3 polypeptide that are important for its function/cell surface expression. PMID: 28637675
    10. RFT2 plays an important role in gastric carcinogenesis by modulating riboflavin absorption PMID: 26722538
    11. C20orf54 rs13042395 polymorphism was significantly associated with decreased ESCC and GCA risk especially for the subjects with under-weight or normal. PMID: 26154995
    12. A close association exists between functional SNP rs3746804 in C20orf54 and susceptibility to esophageal squamous cell carcinoma PMID: 25427582
    13. the single-nucleotide polymorphism rs13042395 in C20orf54 showed a significantly lower risk of esophageal squamous cell carcinoma in the younger age group but no significant association in the older group in a Korean population. PMID: 24152165
    14. Binding of Sp1 to the minimal SLC52A3 promoter. PMID: 25394472
    15. Increase in methylation of CpG 2 and CpG 3 in hRFT2gene promoter region is associated with the genesis of cervical squamous cell carcinoma. PMID: 24761851
    16. Results suggest that RFT2 contributes to esophageal squamous cell carcinoma tumorigenesis and may serve as a potential therapeutic target. PMID: 25045844
    17. C20orf54 expression were significantly up-regulated in CSCC. PMID: 24260322
    18. These results strongly suggest that RFVT3 would functionally be involved in riboflavin absorption in the apical membranes of intestinal epithelial cells PMID: 24264046
    19. Defective expression of C20orf54 is associated with the development of Kazak esophageal squamous cell carcinoma and this may represent a mechanism underlying the decreased plasma riboflavin levels in ESCC. PMID: 23275236
    20. RFT2 protein functional single nucleotide polymorphism might be associated with the development of esophageal squamous cell carcinoma. PMID: 22533825
    21. Identification of novel mutations that affect amino acid changes in Brown-Vialetto-Van Laere syndrome patients. PMID: 22718020
    22. Defective expression of RFT2 is associated with the development of gastric carcinoma and may result in decreased plasma riboflavin levels in GC. PMID: 22791947
    23. Single nucleotide polymorphism in C20orf54 gene is associated with esophageal squamous cell carcinoma. PMID: 22471455
    24. Mutations of riboflavin transporter-2 gene is associated with Brown-Vialetto-Van Laere syndrome. PMID: 22273710
    25. These results demonstrate a potential role for specific cysteine residues in the cell surface expression of riboflavin transporter 2 in human intestinal epithelial cells. PMID: 21512156
    26. results indicate that riboflavin transporter 2(RFT2) is a transporter involved in the epithelial uptake of riboflavin in the small intestine for its nutritional utilization PMID: 20724488
    27. Susceptibility loci at C20orf54 for esophageal squamous cell carcinoma. PMID: 20729853
    28. Discusses cloning of rat riboflavin transporter 2 and identification of a comparable protein in human. PMID: 19122205
    29. identified a candidate gene, C20orf54, in a consanguineous family with Brown-Vialetto-Van Laere syndrome with multiple affected individuals and subsequently demonstrated that mutations in this gene were the cause of disease in other, unrelated families PMID: 20206331

    显示更多

    收起更多

  • 相关疾病：
    Brown-Vialetto-Van Laere syndrome 1 (BVVLS1); Fazio-Londe disease (FALOND)
  • 亚细胞定位：
    Apical cell membrane; Multi-pass membrane protein. Cell membrane.; [Isoform 1]: Cell membrane; Multi-pass membrane protein. Nucleus membrane; Multi-pass membrane protein. Cytoplasm.; [Isoform 2]: Cytoplasm.
  • 蛋白家族：
    Riboflavin transporter family
  • 组织特异性：
    Predominantly expressed in testis. Highly expressed in small intestine and prostate.
  • 数据库链接：

    HGNC: 16187

    OMIM: 211500

    KEGG: hsa:113278

    STRING: 9606.ENSP00000217254

    UniGene: Hs.283865