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  4. Recombinant Human Rho-related GTP-binding protein RhoJ (RHOJ)

Recombinant Human Rho-related GTP-binding protein RhoJ (RHOJ)

  • 中文名称：
    人RHOJ重组蛋白
  • 货号：
    CSB-YP872495HU
  • 规格：
  • 来源：
    Yeast
  • 其他：
  • 中文名称：
    人RHOJ重组蛋白
  • 货号：
    CSB-EP872495HU
  • 规格：
  • 来源：
    E.coli
  • 其他：
  • 中文名称：
    人RHOJ重组蛋白
  • 货号：
    CSB-EP872495HU-B
  • 规格：
  • 来源：
    E.coli
  • 共轭：
    Avi-tag Biotinylated

    E. coli biotin ligase (BirA) is highly specific in covalently attaching biotin to the 15 amino acid AviTag peptide. This recombinant protein was biotinylated in vivo by AviTag-BirA technology, which method is BriA catalyzes amide linkage between the biotin and the specific lysine of the AviTag.

  • 其他：
  • 中文名称：
    人RHOJ重组蛋白
  • 货号：
    CSB-BP872495HU
  • 规格：
  • 来源：
    Baculovirus
  • 其他：
  • 中文名称：
    人RHOJ重组蛋白
  • 货号：
    CSB-MP872495HU
  • 规格：
  • 来源：
    Mammalian cell
  • 其他：

产品详情

  • 纯度：
    >85% (SDS-PAGE)
  • 基因名：
    RHOJ
  • Uniprot No.：
  • 别名：
    ARHJ; Ras homolog family member J; Ras homolog gene family member J; RAS like family 7 member B; Ras like protein family member 7B; Ras-like protein family member 7B; RASL 7B; RASL7B; Rho related GTP binding protein RhoJ; Rho-related GTP-binding protein RhoJ; RHOI; Rhoj; RHOJ_HUMAN; Tc10 like GTP binding protein TCL; TC10 like Rho GTPase; Tc10-like GTP-binding protein; TC10B; TCL
  • 种属：
    Homo sapiens (Human)
  • 蛋白长度：
    full length protein
  • 表达区域：
    1-211
  • 氨基酸序列
    MNCKEGTDSS CGCRGNDEKK MLKCVVVGDG AVGKTCLLMS YANDAFPEEY VPTVFDHYAV TVTVGGKQHL LGLYDTAGQE DYNQLRPLSY PNTDVFLICF SVVNPASYHN VQEEWVPELK DCMPHVPYVL IGTQIDLRDD PKTLARLLYM KEKPLTYEHG VKLAKAIGAQ CYLECSALTQ KGLKAVFDEA ILTIFHPKKK KKRCSEGHSC C
  • 蛋白标签：
    Tag type will be determined during the manufacturing process.
    The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.
  • 产品提供形式：
    Lyophilized powder
    Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
  • 复溶：
    We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
  • 储存条件：
    Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
  • 保质期：
    The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
    Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
  • 货期：
    Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
    Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
  • 注意事项：
    Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
  • Datasheet ：
    Please contact us to get it.

产品评价

靶点详情

  • 功能：
    Plasma membrane-associated small GTPase specifically involved in angiogenesis. Required for endothelial cell migration during vascular development via its interaction with GLUL. Elicits the formation of F-actin-rich structures, thereby regulating endothelial cell migration.
  • 基因功能参考文献：
    1. Short-term treatment of nascent melanoma tumors with PAK inhibitors that block RhoJ signaling halts the growth of BRAF mutant melanoma tumors in vivo and induces apoptosis in melanoma cells in vitro via a BAD-dependent mechanism. As up to 50% of BRAF mutant human melanomas express high levels of RhoJ, these studies nominate the RhoJ-BAD signaling network as a therapeutic vulnerability for fledgling BRAF mutant human tumor PMID: 28753606
    2. These results identify amino acids within the N terminus and a loop region distal to the nucleotide binding pocket of TCL capable of allosterically regulating nucleotide exchange and thus influence membrane association of the protein. PMID: 27660391
    3. FMNL3 interacts with Cdc42 and RhoJ, two Rho family GTPases known to be required for lumen formation. FMNL3 and RhoJ are concentrated at the early apical surface, or AMIS, and regulate the formation of radiating actin cables from this site. PMID: 26299518
    4. RhoJ-knockout mice showed reduced tumour growth and diminished tumour vessel density, identifying a role for RhoJ in mediating tumour angiogenesis. Studies give insight into the molecular function of RhoJ in regulating cell motility. PMID: 24928894
    5. These results identify RhoJ blockade as a selective and effective therapeutic strategy for targeting tumor vasculature with minimal side effects. PMID: 24434213
    6. These observations identify RHOJ as a melanoma linchpin determinant that regulates both actin cytoskeletal dynamics and chemoresistance by activating PAK1. PMID: 23253891
    7. Arhgef15 acts as an endothelial cell-specific GEF to mediate VEGF-induced Cdc42 activation and potentiate RhoJ inactivation, thereby promoting actin polymerization and cell motility. PMID: 23029280
    8. we identified RhoJ and its effector PAK1, as key modulators of melanoma cell sensitivity to DNA damage PMID: 22971344
    9. Our study supports a novel role for the Rho family member RhoJ in ednothelial cell morphogenesis and in particular in lumen formation PMID: 21628409
    10. RhoJ is endothelial-expressed in vivo, activated by vascular endothelial growth factor, localizes to focal adhesions, regulates endothelial cell migration and tube formation, and modulates actomyosin contractility and focal adhesion numbers. PMID: 21148427
    11. TCL is essential for clathrin-dependent endocytosed receptors to enter the early/sorting endosomes PMID: 12960428

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  • 亚细胞定位：
    Cell membrane; Lipid-anchor; Cytoplasmic side.
  • 蛋白家族：
    Small GTPase superfamily, Rho family
  • 组织特异性：
    Specifically expressed in endothelial cells in different tissues, such as brain, heart, lung and liver.
  • 数据库链接：

    HGNC: 688

    OMIM: 607653

    KEGG: hsa:57381

    STRING: 9606.ENSP00000316729

    UniGene: Hs.656339