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  4. Recombinant Human Protein phosphatase 1 regulatory subunit 1B (PPP1R1B)

Recombinant Human Protein phosphatase 1 regulatory subunit 1B (PPP1R1B)

  • 货号：
    CSB-YP880070HU
  • 规格：
  • 来源：
    Yeast
  • 其他：
  • 货号：
    CSB-EP880070HU-B
  • 规格：
  • 来源：
    E.coli
  • 共轭：
    Avi-tag Biotinylated

    E. coli biotin ligase (BirA) is highly specific in covalently attaching biotin to the 15 amino acid AviTag peptide. This recombinant protein was biotinylated in vivo by AviTag-BirA technology, which method is BriA catalyzes amide linkage between the biotin and the specific lysine of the AviTag.

  • 其他：
  • 货号：
    CSB-BP880070HU
  • 规格：
  • 来源：
    Baculovirus
  • 其他：
  • 货号：
    CSB-MP880070HU
  • 规格：
  • 来源：
    Mammalian cell
  • 其他：

产品详情

  • 纯度：
    >85% (SDS-PAGE)
  • 基因名：
  • Uniprot No.：
  • 别名：
    DARPP 32; DARPP-32; Dopamine and cAMP regulated neuronal phosphoprotein 32; Dopamine and cAMP regulated neuronal phosphoprotein; Dopamine and cAMP regulated phosphoprotein; Dopamine and cAMP regulated phosphoprotein DARPP 32 ; Dopamine and cAMP regulated phosphoprotein DARPP32; Dopamine- and cAMP-regulated neuronal phosphoprotein; FLJ20940; IPPD; Neuronal phosphoprotein DARPP 32; PPP1R1B; PPR1B_HUMAN; Protein phosphatase 1 regulatory (inhibitor) subunit 1B; Protein phosphatase 1 regulatory inhibitor subunit 1B; Protein phosphatase 1 regulatory subunit 1B
  • 种属：
    Homo sapiens (Human)
  • 蛋白长度：
    full length of Isoform 2
  • 表达区域：
    1-168
  • 氨基酸序列
    MLFRLSEHSSPEEEASPHQRASGEGHHLKSKRPNPCAYTPPSLKAVQRIAESHLQSISNLNENQASEEEDELGELRELGYPREEDEEEEEDDEEEEEEEDSQAEVLKVIRQSAGQKTTCGQGLEGPWERPPPLDESERDGGSEDQVEDPALSEPGEEPQRPSPSEPGT
  • 蛋白标签：
    Tag type will be determined during the manufacturing process.
    The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.
  • 产品提供形式：
    Lyophilized powder
    Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
  • 复溶：
    We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
  • 储存条件：
    Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
  • 保质期：
    The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
    Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
  • 货期：
    Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
    Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
  • 注意事项：
    Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
  • Datasheet ：
    Please contact us to get it.

产品评价

靶点详情

  • 功能：
    Inhibitor of protein-phosphatase 1.
  • 基因功能参考文献：
    1. t-Darpp phosphorylation at T39 seems to be crucial for t-Darpp-mediated PKA activation and this activation appears to occur through an association with RI and sequestering of RI away from PKAc. The t-Darpp-RI interaction could be a druggable target to reduce PKA activity in drug-resistant cancer. PMID: 28867659
    2. our results lend support for a potential role of DARPP-32 genetic variants in neural response to potential reward triggering cues PMID: 29048604
    3. To facilitate live-cell identification, a human embryonic stem cell line was generated using CRISPR-mediated gene editing at the DARPP32 locus. PMID: 28065797
    4. Study associates polymorphisms in DRD2, DARPP-32, and COMT genes with novel category learning performance. Modeling results suggest that striatal dopaminergic genes influence selective attention processes whereas cortical genes mediate the ability to update complex rule representations. PMID: 26918585
    5. Results show that expression of human DARPP-32 protein isoforms depends on the striatal neurodevelopmental stage with t-DARPP being specific for the human adult striatum. PMID: 27475250
    6. In vivo xenograft models demonstrated that overexpression of DARPP-32 promotes angiogenesis and tumour growth. PMID: 25779598
    7. This study strongly suggests that inflammation-regulated DARPP-32 constitutes a key component of H. pylori-mediated gastric tumourigenesis. PMID: 27590997
    8. This study did not find any statistical difference in the state-anxiety dimension for the rs12601930C/T, rs879606A/G, and rs3764352A/G genotypes, but statistical differences in the trait-anxiety dimension for the rs879606A/G (F = 5.207, P = 0.006) and rs3764352A/G (F = 3.960, P = 0.020) genotype. PMID: 27995567
    9. DARPP-32 is mainly cleaved at Thr(153) by calpain and this cleavage of DARPP-32 reduces CREB phosphorylation via loss of its inhibitory function on PP1; results suggest a novel mechanism of DARPP-32-CREB signalling dysregulation in Alzheimer's disease PMID: 26178297
    10. No significant differences in mRNA expression levels of DRD2 and DARPP-32 were found among controls, patients with psychotic disorder not otherwise specified, and schizophrenia/schizophreniform disorder. PMID: 26561806
    11. Striatal function was indexed by a gene coding for DARPP-32, which is densely expressed in the striatum where it is necessary for synaptic plasticity PMID: 26818509
    12. Findings show that PPP1R1B-STARD3 fusion transcript has a key role in subsets of gastric cancers through the activation of PI3K/AKT signaling. PMID: 24276243
    13. The results of this study found that the mean t-DARPP expression level in the caudate was higher in patients with schizophrenia than in control individuals. PMID: 24704945
    14. Results suggest that variation in PPP1R1B affects the abundance of the splice variant t-DARPP-32 mRNA and may reflect potential molecular mechanisms implicated in schizophrenia and affective disorders PMID: 23295814
    15. The immunostaining was more decreased for DARPP-32 than for the morphological changes of striatal neurons in multiple system atrophy. PMID: 23715974
    16. This study showed that variations in genotypes relevant for DARPP-32 functions are associated more with aging-related impairments in the explicit than the implicit component of sequence learning. PMID: 24035787
    17. density of DARPP-32-immunoreactive (IR) neurons in the II and III layers of the RAIC was significantly decreased (p<0.05) in the schizophrenia group compared with the healthy control group. PMID: 21821092
    18. Bcl-2 and DARPP-32 have roles in regulating inositol 1,4,5-trisphosphate receptor phosphorylation and promoting cell survival PMID: 24395794
    19. High DARPP32/STAT3, DARPP32/STAT5B, and STAT5B/STAT3 ratios were associated with longer patient progression free survival. PMID: 23250732
    20. The present study provides novel evidence for an association between the DARPP-32 gene (SNP rs907094) and attentional control of auditory perception. PMID: 23639477
    21. Findings show a novel mechanism by which DARPP-32 promotes cell invasion by regulating CXCR4-mediated activation of the MT1-MMP/MMP-2 pathway. PMID: 23160836
    22. PPP1R1B rs12601930 was associated with projection and splitting. rs3764352 was associated with splitting. No significant association was found between rs879606 and defenses. PPP1R1B polymorphisms are involved in immature defenses. PMID: 23080070
    23. Our findings support a role for the DRD2 and PPP1R1B genes in conferring risk for autism in families with only affected males and show an additive effect of these genes towards prediction of affected status in our families. PMID: 22559203
    24. Our findings uncover a novel mechanism of TRAIL resistance mediated by DARPP-32, whereby it inhibits the intrinsic apoptosis pathway through upregulation of BCL-xL PMID: 22589394
    25. DARPP32 has a role in pluripotent stem cell differentiation toward medium-sized spiny neurons PMID: 23250204
    26. no significant association of BDNF or DARPP-32 polymorphisms with schizophrenia in Malays. PMID: 22576830
    27. the decrease in DARPP-32 in schizophrenia was more marked in neurons of dorsolateral prefrontal cortex than in other cells or other brain regions PMID: 22179181
    28. DARPP-32 increases interactions between epidermal growth factor receptor and ERBB3 to promote tumor resistance to gefitinib. PMID: 21741919
    29. [review] Genetic polymorphisms modulating DARPP-32 mRNA expression and cognition in humans are associated with changes in activation in the entire striatum, and striatal connectivity with frontal cortex, without connection to any other brain region. PMID: 20631684
    30. the DARPP-32-related pathogenesis in schizophrenia may be more severe in the superior temporal gyrus than previously found in the prefrontal cortex PMID: 21453742
    31. Results suggest that polymorphisms in the DARPP-32 gene are involved in the biological mechanisms that confer the traits of novelty seeking and harm avoidance. PMID: 21369787
    32. Findings demonstrate that t-DARPP regulates beta-catenin/TCF activity, thereby implicating a novel oncogenic signaling in upper gastrointestinal cancers. PMID: 21447180
    33. our data suggest: (i) C14ORF28, GNB2L1, MLLT3, DRD2 and DARPP-32 are important in the pathogenesis of schizophrenia and bipolar disorder PMID: 20874815
    34. Findings underscore the potential role of t-DARPP in regulating cell growth and proliferation through PI3 kinase-dependent mechanism. PMID: 20836878
    35. This study did not find DAOA significant associations with schizophrenia. Thus, PPP1R1B genes do not fit the antagonistic pleiotropy PMID: 20483474
    36. The composite expression score, calculated from immunostaining patterns, increased significantly from normal or gastritis to metaplasia, dysplasia, and adenocarcinoma (P < .001). PMID: 20580047
    37. DARPP-32 changes the usage of tra2-beta1 dependent alternative exons in a concentration-dependent manner, suggesting that the DARPP-32:tra2-beta1 interaction is a molecular link between signaling pathways and pre-mRNA processing. PMID: 20074680
    38. t-Darpp and DARPP-32 expression are novel prognostic and predictive biomarkers in breast cancer. PMID: 19301121
    39. DARPP 32 frequently are overexpressed in common subtypes of human adenocarcinomas suggest that these proteins may be important in tumorigenesis. PMID: 14508844
    40. overexpression of DARPP-32 is associated with gastric cancer PMID: 14991576
    41. DARPP-32 expression arises after a phase of dysplasia esophageal squamous cell carcinoma; tumours expressing DARPP-32 progress less rapidly than DARPP-32-negative tumours PMID: 15188007
    42. multiple domains in I-1 target cellular PP1 complexes, and I-1 has a role as a cellular regulator of eIF2alpha phosphorylation PMID: 15345721
    43. Expression of Darpp-32 is associated with a potent antiapoptotic advantage for cancer cells through a p53-independent mechanism that involves preservation of mitochondrial potential and increased Bcl2 levels. PMID: 16061638
    44. Thus, DARPP-32 signaling downstream of DDR1 is a potential new target for effective anti-metastatic breast cancer therapy PMID: 17027969
    45. Potential involvement of PPP1R1B in the etiology of nicotine dependence. PMID: 17171661
    46. DARPP-32 plays a pivotal role in cognitive function and possibly in the pathogenesis of schizophrenia. PMID: 17290303
    47. The up-regulation of ZNRD1 significantly inhibited the drug sensitivity of gastric cancer cells over-expressing DARPP-32, indicating that ZNRD1 may be important in the DARPP-32-mediated MDR of gastric cancer PMID: 17492506
    48. The present study suggests that DARPP-32 decreases in the DLPFC of patients with schizophrenia and bipolar disorder. PMID: 17521792
    49. The results of this study do not preclude the possibility that the PPP1R1B is a susceptibility gene for schizophrenia in the Chinese population. PMID: 17618027
    50. The decreased expression of DARPP-32 in oral premalignant and malignant lesions suggests a tumor suppressor role for this protein in the tumorigenesis of these lesions. PMID: 17695523

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  • 亚细胞定位：
    Cytoplasm.
  • 蛋白家族：
    Protein phosphatase inhibitor 1 family
  • 数据库链接：

    HGNC: 9287

    OMIM: 604399

    KEGG: hsa:84152

    STRING: 9606.ENSP00000254079

    UniGene: Hs.286192