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  4. Recombinant Human Protein Wnt-2 (WNT2)

Recombinant Human Protein Wnt-2 (WNT2)

  • 货号：
    CSB-MP026133HU
  • 规格：
  • 来源：
    Mammalian cell
  • 其他：
  • 货号：
    CSB-EP026133HU-B
  • 规格：
  • 来源：
    E.coli
  • 共轭：
    Avi-tag Biotinylated

    E. coli biotin ligase (BirA) is highly specific in covalently attaching biotin to the 15 amino acid AviTag peptide. This recombinant protein was biotinylated in vivo by AviTag-BirA technology, which method is BriA catalyzes amide linkage between the biotin and the specific lysine of the AviTag.

  • 其他：

产品详情

  • 纯度：
    >85% (SDS-PAGE)
  • 基因名：
  • Uniprot No.：
  • 别名：
    Int 1 related protein; Int-1-like protein 1; Int-1-related protein; INT1L 1; INT1L1; IRP; IRP protein; ONCOGENE INT1 LIKE 1; Protein Wnt-2; Secreted growth factor; Wingless type MMTV integration site family member 2; WNT 2; WNT2; WNT2_HUMAN
  • 种属：
    Homo sapiens (Human)
  • 蛋白长度：
    Full Length of Mature Protein
  • 表达区域：
    26-360aa
  • 氨基酸序列
    SWWYMRATGGSSRVMCDNVPGLVSSQRQLCHRHPDVMRAISQGVAEWTAECQHQFRQHRWNCNTLDRDHSLFGRVLLRSSRESAFVYAISSAGVVFAITRACSQGEVKSCSCDPKKMGSAKDSKGIFDWGGCSDNIDYGIKFARAFVDAKERKGKDARALMNLHNNRAGRKAVKRFLKQECKCHGVSGSCTLRTCWLAMADFRKTGDYLWRKYNGAIQVVMNQDGTGFTVANERFKKPTKNDLVYFENSPDYCIRDREAGSLGTAGRVCNLTSRGMDSCEVMCCGRGYDTSHVTRMTKCGCKFHWCCAVRCQDCLEALDVHTCKAPKNADWTTAT
  • 蛋白标签：
    Tag type will be determined during the manufacturing process.
    The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.
  • 产品提供形式：
    Lyophilized powder
    Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
  • 复溶：
    We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
  • 储存条件：
    Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
  • 保质期：
    The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
    Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
  • 货期：
    Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
    Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
  • 注意事项：
    Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
  • Datasheet ：
    Please contact us to get it.

产品评价

靶点详情

  • 功能：
    Ligand for members of the frizzled family of seven transmembrane receptors. Functions in the canonical Wnt signaling pathway that results in activation of transcription factors of the TCF/LEF family. Functions as upstream regulator of FGF10 expression. Plays an important role in embryonic lung development. May contribute to embryonic brain development by regulating the proliferation of dopaminergic precursors and neurons.
  • 基因功能参考文献：
    1. Down-regulation of miR-30a-3p/5p promotes esophageal squamous cell carcinoma cell proliferation by activating the Wnt signaling pathway through inhibition of Wnt2 and Fzd2. PMID: 29259372
    2. The data suggest that Med19 expression correlates with aggressive characteristics of bladder cancer, and Med19 knockdown suppresses the proliferation and migration of cancer cells through down-regulating the Wnt/beta-catenin pathway. PMID: 28631286
    3. The findings of this study have shown that molecular mechanisms, including aberrant activation of the WNT2 gene signaling pathway, may be involved in the pathogenesis of preeclampsia. PMID: 29109390
    4. SNPs in WNT2 and FOXP2 are associated with clinical symptom severity of autism spectrum disorders. PMID: 28081867
    5. WNT2 insufficiency might cause impaired trophoblast cell proliferation and migration via downregulation of Wnt/beta-catenin signaling pathway. PMID: 28627774
    6. Three-dimensional organotypic co-culture assays revealed that WNT2-mediated fibroblast motility and extracellular matrix remodeling enhanced cancer cell invasion of cell lines even harboring mutations in the adenomatous polyposis coli/beta-catenin pathway. PMID: 28553956
    7. Chondrogenic potential was higher and Wnt/beta-catenin signaling was more potently activated by a GSK-3beta inhibitor in the posterior than in the anterior part of the human infant sclera. PMID: 27336854
    8. data enforced the evidence that knockdown of c-Fos inhibited cell proliferation, migration, and invasion, and promoted the apoptosis of OS cells accompanied by altered expression of Wnt2 and Fzd9 PMID: 28665975
    9. High WNT2 expression is associated with Cervical Carcinoma Metastasis. PMID: 27513465
    10. targeting beta-catenin with lipofection-delivered small interfering RNA oligonucleotide inhibited the proliferation and cell cycle arrest in G0/G1 phase and increased apoptosis of fibroblast cells, accompanied by downregulation of Wnt2 and cyclin D1 as well as the phosphorylation level of glycogen synthase kinase 3 beta in the keloid fibrosis PMID: 28656880
    11. Genetic polymorphisms in the WNT2 and CTNNB1 genes were closely associated with hepatocellular carcinoma risk and progression in a Chinese Han population PMID: 28328801
    12. The WNT2 rs39315 G allele was associated with advanced tumor stage in hepatocellular carcinoma. PMID: 26968103
    13. Wnt2 complements Wnt/beta-catenin signaling in regulating colorectal cancer cell proliferation. PMID: 26484565
    14. Wnt2/beta-catenin signaling was involved in stromal-epithelial cells interaction in endometriosis. PMID: 26116230
    15. High expression of Wnt2 is associated with pancreatic cancer progression promoted by pancreatic stellate cells. PMID: 25731618
    16. Hypermethylation of WNT2 is associated with colorectal cancer. PMID: 23694962
    17. The study demonistrated that expressions of Wnt-2 in high-grade brainstem gliomas were significantly higher than that in low-grade brainstem gliomas and normal brain tissues and were positively correlated with the expression of Ki-67. PMID: 23603171
    18. Decreased placental expression of Wnt2 and increased placental expression of sFRP4 may be associated with the pathogenesis of severe preeclampsia. PMID: 23322712
    19. We demonstrated an activation of Wnt-2 signaling via the Frizzled-8 receptor in non-small cell lung cancer cells PMID: 23815780
    20. The skin lesions of scleroderma patients showed obviously increased expression of cytoplasmic Wnt 2, nuclear Wnt 3a and beta-catenin, but markedly lowered cell membrane expression of beta-catenin than normal skin. PMID: 23268410
    21. significant association with SNP rs4730775 locus on chromosome 7 with genetic susceptibility to Peyronie's disease PMID: 22489561
    22. Pdgf signaling potentiates Wnt2-Wnt7b signaling to promote high levels of Wnt activity in mesenchymal progenitors that is required for proper development of endoderm-derived organs, such as the lung PMID: 22949635
    23. The WNT2 gene may play a suggestive role in language development in autistic disorder. PMID: 22522212
    24. Expression of WNT2 in pancreatic cancer cells suppresses anoikis, enhances anchorage-independent sphere formation, and increases metastatic propensity in vivo. PMID: 22763454
    25. Wnt/beta-catenin pathway forms a negative feedback loop during TGF-beta1 induced human normal skin fibroblast-to-myofibroblast transition PMID: 22041457
    26. a haplotype of WNT2 (rs2896218-rs6950765: G-G) is significantly associated with ASDs. PMID: 21575668
    27. DNA methylation within the WNT2 promoter in human placenta is associated with low birthweight. PMID: 21474991
    28. findings provide evidence for a significant association between WNT2 and autism. PMID: 19895723
    29. The present study does not support a major role for WNT2 in schizophrenia. PMID: 20492734
    30. Wnt2/beta-catenin signaling pathway is constitutively activated in esophageal squamous cell carcinoma (ESCC) cells, indicating that Wnt2/beta-catenin pathway plays an essential role in carcinogenesis of the esophagus. PMID: 19857041
    31. Wnt2 acts as an angiogenic factor for non-sinusoidal endothelium in vitro and in vivo. PMID: 19444628
    32. Up-regulation of WNT2 mRNA by estrogen might play a key role in some cases of human breast cancer through activation of the beta-catenin - TCF signaling pathway. PMID: 11712082
    33. Activating mutations of the WNT2 gene are not a major contributor to the development of autistic disorder. PMID: 11840514
    34. REVIEW: role in human gastrointestinal cancer PMID: 14533014
    35. Our interpretation of these findings is that it is unlikely that DNA variations in RELN and WNT2 play a significant role in the genetic predisposition to autism. PMID: 15048648
    36. Target genes involved in tumor WNT pathways in response to TGF-beta1 were found in cervix cancer cells. PMID: 15878915
    37. Wnt/beta-catenin signalling pathway is activated in most of gastric cancers, which may play pivotal roles either in gastric cancer formation or in tumour invasion and dissemination PMID: 15896469
    38. Inhibition of Wnt2 by monoclonal antibodies is of therapeutic interest in the development of more effective treatments for malignnt pleueral mesothelioma. PMID: 15900580
    39. The increased Wnt2 expression in gastric cancers is paralleled with reduction of membranous E-cadherin. PMID: 16132582
    40. The WNT2 gene is upregulated along the progression from low-grade dysplasia to esophageal adenocarcioma. PMID: 16407829
    41. Regulation of Fz4 expression by Wnt2. PMID: 17386109
    42. inhibition of both Wnt-2 and Gal-3 had synergistic effects on suppressing canonical Wnt signaling and inducing apoptosis, suggesting that aberrant canonical Wnt/beta-catenin signaling in colorectal cancer can be regulated at multiple levels PMID: 17534895
    43. potential coordinative effects of Wnt, NF-kappaB and/or Stat3 activation on gastric cancer formation presumably by promoting the transcription of their common target genes. PMID: 18184402
    44. Persistent upregulation in colorectal carcinoma cases studied. PMID: 18600204
    45. Wnt (Wnt2), Stat3, and Notch-1 and -2 signaling are correlated in human epidermal tumors. PMID: 18703315
    46. Knockdown of Wnt2 by siRNA in human U251 glioma cells inhibited cell proliferation and invasive ability, and induced apoptotic cell death PMID: 18949017
    47. The results suggest that WNT2 could act through its receptor FZD9 to regulate the beta-CATENIN pathway in cumulus cells, recruiting beta-CATENIN into plasma membranes and promoting the formation of adherens junctions involving CDH1. PMID: 19038973
    48. up-regulation of the Wnt-2 protein might play a role in the development of colorectal cancers. PMID: 19239325
    49. RNA samples from 21 neuroblastoma showed a highly significant FZD1 and/or MDR1 overexpression after treatment, underlining a role for FZD1-mediated Wnt/beta-catenin pathway in clinical chemoresistance. PMID: 19421142

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  • 亚细胞定位：
    Secreted, extracellular space, extracellular matrix. Secreted.
  • 蛋白家族：
    Wnt family
  • 组织特异性：
    Expressed in brain in the thalamus, in fetal and adult lung and in placenta.
  • 数据库链接：

    HGNC: 12780

    OMIM: 147870

    KEGG: hsa:7472

    STRING: 9606.ENSP00000265441

    UniGene: Hs.567356