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  4. Recombinant Human Protein-S-isoprenylcysteine O-methyltransferase (ICMT), partial

Recombinant Human Protein-S-isoprenylcysteine O-methyltransferase (ICMT), partial

  • 货号：
    CSB-YP010956HU
  • 规格：
  • 来源：
    Yeast
  • 其他：
  • 货号：
    CSB-EP010956HU
  • 规格：
  • 来源：
    E.coli
  • 其他：
  • 货号：
    CSB-EP010956HU-B
  • 规格：
  • 来源：
    E.coli
  • 共轭：
    Avi-tag Biotinylated

    E. coli biotin ligase (BirA) is highly specific in covalently attaching biotin to the 15 amino acid AviTag peptide. This recombinant protein was biotinylated in vivo by AviTag-BirA technology, which method is BriA catalyzes amide linkage between the biotin and the specific lysine of the AviTag.

  • 其他：
  • 货号：
    CSB-BP010956HU
  • 规格：
  • 来源：
    Baculovirus
  • 其他：
  • 货号：
    CSB-MP010956HU
  • 规格：
  • 来源：
    Mammalian cell
  • 其他：

产品详情

  • 纯度：
    >85% (SDS-PAGE)
  • 基因名：
    ICMT
  • Uniprot No.：
  • 别名：
    1700008E11Rik; C80758; fcmt; Gm13095; HSTE14; Icmt; ICMT_HUMAN; Isoprenylcysteine carboxylmethyltransferase; MGC118506; MGC39955 ; MGC95322; MST098; MSTP098; OTTMUSG00000010406; pcCMT; PCMT; PPMT; Prenylated protein carboxyl methyltransferase; Prenylcysteine carboxyl methyltransferase; Protein-S-isoprenylcysteine O-methyltransferase; RP23-421E12.4; STE14
  • 种属：
    Homo sapiens (Human)
  • 蛋白长度：
    Partial
  • 蛋白标签：
    Tag type will be determined during the manufacturing process.
    The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.
  • 产品提供形式：
    Lyophilized powder
    Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
  • 复溶：
    We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
  • 储存条件：
    Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
  • 保质期：
    The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
    Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
  • 货期：
    Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
    Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
  • 注意事项：
    Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
  • Datasheet ：
    Please contact us to get it.

产品评价

靶点详情

  • 功能：
    Catalyzes the post-translational methylation of isoprenylated C-terminal cysteine residues.
  • 基因功能参考文献：
    1. Using cell culturing system, mouse model and patient samples, this work is the first to demonstrate the essential roles of Icmt in ovarian cancer via Ras signaling, particularly on its response to chemotherapy. PMID: 29746868
    2. Apoptosis, but not autophagy, that is induced via p21-activated BNIP3 expression accounts for the efficacy of ICMT inhibition in sensitive pancreatic cancer cells in both in vitro and in vivo models. In contrast, cells resistant to ICMT inhibition demonstrated no mitochondria dysfunction or p21 signaling changes under ICMT suppression PMID: 28167504
    3. ICMT catalyzed carboxylmethylation is critical for RAB4A activation and interaction with effectors, its localization to endosomes and recycling vesicles, and hence important for RAB4A-dependent integrin beta3 recycling to plasma membrane. PMID: 28604748
    4. ICMT function is critical for the tumorigenesis capacity of naturally occurring mutant KRAS human cancer cells. PMID: 28192404
    5. simultaneous inhibition of BCR-ABL1 and Icmt may represent a potential therapeutic strategy for CML. PMID: 26706195
    6. impact of Icmt on tumorigenic processes PMID: 25151967
    7. Silencing ICMT in osteosarcoma cells decreased Notch1 signaling in response to stimulation with cell-surface ligands. PMID: 24216479
    8. a role for isoprenylcysteine carboxylmethyltransferase in cell migration and adhesion. PMID: 20798596
    9. modulation of pcCMT activity in retinoic acid (RA)-treated SH-SY5Y neuroblastoma cells PMID: 11906819
    10. ICMTase is a critical component of the redox-sensitive VCAM-1-selective signaling pathway and appears to activate a discrete inflammatory signaling pathway, at least in part, through the methylation of Rac1 in endothelial cells. PMID: 12006387
    11. findings suggest that inhibition of isoprenylcysteine-O-carboxyl methyltransferase (ICMT) causes endothelial cell apoptosis by attenuation of Ras GTPase methylation and activation and its downstream antiapoptotic signaling pathway. PMID: 12631708
    12. These studies identify isoprenylcysteine carboxyl methyltransferase as a potential target for reducing the growth of K-Ras- and B-Raf-induced malignancies. PMID: 14966563
    13. ICMT inhibition induces endothelial cell apoptosis through GRP94 PMID: 17347446
    14. Icmt traverses the Eendoplasmic reticulum membrane eight times, with both N and C termini disposed toward the cytosol and with a helix-turn-helix structure comprising transmembrane (TM) segments 7 and 8. PMID: 19158273
    15. Inhibition of isoprenylcysteine carboxylmethyltransferase (Icmt) decreases activation of RhoA and Rac1, and in the absence of Icmt activity, addition of exogenous RhoA or Rac1 partially rescues directed and random migration, respectively. PMID: 19651782

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  • 亚细胞定位：
    Endoplasmic reticulum membrane; Multi-pass membrane protein.
  • 蛋白家族：
    Class VI-like SAM-binding methyltransferase superfamily, Isoprenylcysteine carboxyl methyltransferase family
  • 组织特异性：
    Ubiquitously expressed. Expressed at higher levels in the cerebellum and putamen than in other brain regions. Abundant expression seen in the Purkinje cells and pontine neurons.
  • 数据库链接：

    HGNC: 5350

    OMIM: 605851

    KEGG: hsa:23463

    STRING: 9606.ENSP00000343552

    UniGene: Hs.515688