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  4. Recombinant Human Phenylalanine-4-hydroxylase (PAH)

Recombinant Human Phenylalanine-4-hydroxylase (PAH)

In Stock
  • 货号：
    CSB-EP017396HU
  • 规格：
    ￥1344
  • 图片：
    • (Tris-Glycine gel) Discontinuous SDS-PAGE (reduced) with 5% enrichment gel and 15% separation gel.
  • 其他：

产品详情

  • 纯度：
    Greater than 90% as determined by SDS-PAGE.
  • 基因名：
  • Uniprot No.：
  • 别名：
    PAH; PH; PH4H_HUMAN; Phe 4 monooxygenase; Phe-4-monooxygenase; Phenylalanine 4 hydroxylase; Phenylalanine hydroxylase; Phenylalanine-4-hydroxylase; PKU; PKU1
  • 种属：
    Homo sapiens (Human)
  • 蛋白长度：
    Full Length of Mature Protein
  • 来源：
    E.coli
  • 分子量：
    67.7kDa
  • 表达区域：
    2-452aa
  • 氨基酸序列
    STAVLENPGLGRKLSDFGQETSYIEDNCNQNGAISLIFSLKEEVGALAKVLRLFEENDVNLTHIESRPSRLKKDEYEFFTHLDKRSLPALTNIIKILRHDIGATVHELSRDKKKDTVPWFPRTIQELDRFANQILSYGAELDADHPGFKDPVYRARRKQFADIAYNYRHGQPIPRVEYMEEEKKTWGTVFKTLKSLYKTHACYEYNHIFPLLEKYCGFHEDNIPQLEDVSQFLQTCTGFRLRPVAGLLSSRDFLGGLAFRVFHCTQYIRHGSKPMYTPEPDICHELLGHVPLFSDRSFAQFSQEIGLASLGAPDEYIEKLATIYWFTVEFGLCKQGDSIKAYGAGLLSSFGELQYCLSEKPKLLPLELEKTAIQNYTVTEFQPLYYVAESFNDAKEKVRNFAATIPRPFSVRYDPYTQRIEVLDNTQQLKILADSINSEIGILCSALQKIK
    Note: The complete sequence including tag sequence, target protein sequence and linker sequence could be provided upon request.
  • 蛋白标签：
    N-terminal 6xHis-SUMO-tagged
  • 产品提供形式：
    Liquid or Lyophilized powder
    Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
  • 缓冲液：
    Tris-based buffer,50% glycerol
  • 储存条件：
    Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
  • 保质期：
    The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
    Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
  • 货期：
    3-7 business days
  • 注意事项：
    Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
  • Datasheet & COA：
    Please contact us to get it.

产品评价

靶点详情

  • 功能：
    Catalyzes the hydroxylation of L-phenylalanine to L-tyrosine.
  • 基因功能参考文献：
    1. Molecular diagnostics for DNAJC12 variants are thus mandatory in all patients in which deficiencies of PAH and BH4 are genetically excluded. PMID: 29174366
    2. Mutational spectrum of the phenylalanine hydroxylase gene in patients with phenylketonuria in the central region of China has been reported. PMID: 29390883
    3. a spectrum of PAH mutations complied from 35 PKU children who are all Chinese Han population from north Jiangsu, is reported in this study. PMID: 29413232
    4. strong association between particular mutations and minihaplotypes could be useful for prenatal diagnosis and preimplantation genetic diagnosis in affected families PMID: 28676969
    5. PAH mutation was associated with hyperphenylalaninemia. PMID: 29032371
    6. Report a novel intron 11 regulatory element, which is involved in exon 11 splicing, as revealed by the investigated pathogenic effect of variants c.1199+17G>A and c.1199+20G>C, identified in PKU patients. Both mutations cause exon 11 skipping in a minigene system. PMID: 29684050
    7. three novel variants of the PAH gene, p.E178K (c.532G>A), p.V245M (c.733G>A), p.S250F (c.749C>T), showed impaired protein expression and enzyme activity. PMID: 29653233
    8. Among phenylketonuria patients, some with autism at the time of evaluation, six mutations were identified: p.E280K, p.G352Vfs, IVS10nt11, p.I224T, p.R261Q, and p.R252W. Study found no correlation between autism and mutations affecting the phenylalanine hydroxylase gene, but the age of die PMID: 26759449
    9. Studies involving co-expression of differently phenylalanine hydroxylase (PAH) alleles have shown that one variant form can influence the other when assembled into a tetramer and have effect on enzyme activity in vitro, and on BH4 responsiveness and PKU phenotype in patients carrying them. [review] PMID: 26919687
    10. 17 VUS (37%; 7 in ACADM, 9 in GALT, and 1 in PAH) were reclassified from uncertain (6 to benign or likely benign and 11 to pathogenic or likely pathogenic). We identified common types of missing information that would have helped make a definitive classification and categorized this information by ease and cost to obtain PMID: 27308838
    11. Our findings contribute to better understanding of structure and function of PAH mutated enzymes and optimal treatment of PKU patients carrying these mutations using BH4 supplementation. PMID: 28653649
    12. We obtained a PAH gene variant spectrum for the Northern Chinese population and devised a strategy for gene diagnosis using phenylketonuria pedigrees. PMID: 28982351
    13. Phenylalanine hydroxylase gene mutations are associated with phenylketonuria. PMID: 28604955
    14. DNA methylated alleles of the phenylalanine hydroxylase promoter remodeled at elevated phenylalanine levels in newborns with hyperphenylalaninemia have been characterized. PMID: 28389235
    15. In this study, we assigned the phenotypic outcome of three of the five novel mutations and furthermore six not previously classified mutations to one of the four PKU categories. PMID: 26542770
    16. PAH mutation analyses provided further support for genotype-phenotype correlations in patients with hyperphenylalaninemia. The high incidence of phenylketonuria in Nagasaki, the westernmost part of Japan, might be due to migration of people with PAH mutations from China and Korea, and geographic factors. PMID: 27173423
    17. Our study highlighted two novel promoter KLF1 and 3'-region C/EBPalpha motifs in the phenylalanine hydroxylase (PAH) gene which decrease transcription in vitro and, thus, could be considered as PAH expression modifiers. PMID: 27447460
    18. The results of the in vitro residual PAH activity have major implications, both for our understanding of genotype-phenotype correlations, and thereby existing inconsistencies, but also for the elucidation of the molecular basis of tetrahydrobiopterin (BH4) responsiveness. PMID: 27620137
    19. Data provide the structural evidence for a dietary I-phenylalanine (Phe) binding pocket at the subunit-subunit interface of a N-terminal regulatory domain (PAH-RD) dimer, and demonstrate that PAH-RD dimerization depends on Phe binding. PMID: 27049649
    20. The co-expression of two distinct PAH variants revealed possible dominance effects (positive or negative) by one of the variants on residual PAH activity as a result of interallelic complementation. PMID: 26803807
    21. PAH gene mutation analysis combined with STR linkage analysis can provide rapid and accurate prenatal diagnosis for phenylketonuria families PMID: 26600521
    22. The mutation spectrum of PAH gene in Henan seems to differ from that of other regions. Independent assortment of mutant alleles may result in a complex genotype-phenotype correlation. PMID: 27264808
    23. This study identified one novel PAH variant-c.699C>G-and and tries to show a genotype-phenotype relationship also regarding BH4-responsiveness. PMID: 25894915
    24. This study is the first report on tested population genetic structure using VNTR alleles at the PAH gene PMID: 26025954
    25. Aberrant methylation is observed in leukocytes of PAH deficient phenylketonuria patients and is influenced by phenylalanine exposure. PMID: 25990862
    26. We determined phenylalanine hydroxylase function of 30 frequent homozygous and compound heterozygous genotypes covering 55% of the study population. PMID: 25596310
    27. Mutational spectrum was presented for PAH gene in PAH deficiency patients from different parts of Mexico. New mutations were described. PMID: 24941924
    28. mutation spectrum of the gene PAH in the Qinghai population was similar to that in other populations in North China PMID: 26575882
    29. Combining in silico analysis and molecular dynamics simulations (in total 3 mus) we described the structural impact of the mutations, which allowed us to separate 32 out of 34 mutations between groups A and B. PMID: 25750018
    30. 15 different mutations of phenylalanine hydroxylase gene were detected in patients with phenylketonuria. PMID: 25863075
    31. R241C, R408Q and Ex6-96A>G are the most common mutations in PAH in phenylalanine hydroxylase deficiency patients. PMID: 25863076
    32. 15 different mutations were found in 27 unrelated Kurdish PKU patients. IVS4 + 1G > C (c.441 + 1G > C) and IVS7 - 5 T > C (c.843 - 5 T > C) are novel mutations. PAH mutations differ between the Kermanshah province and other parts of Iran. PMID: 24048906
    33. We demonstrated the high expression of PAH and a large increase of PAH activity in differenciated liver progenitor cells. PMID: 24825084
    34. findings suggest that common genetic variations in Phenylalanine hydroxylase are associated with verbal memory in healthy adults. PMID: 23898865
    35. In this study of the PAH mutation spectrum in the Taiwanese population, 139 alleles were identified including 34 different mutations. PMID: 24401910
    36. Mutations were detected in the exons and flaking introns of PAH gene of 44 families with classical phenylketonuria. PMID: 25449068
    37. lipoprotein synthesis in PAH-deficient children, particularly in PKU children, was suppressed in early life. PMID: 24607329
    38. Two polymorphic variants of PAH appear to be risk factors for NSCL/P, rs7485331 and rs12425434 in a Polish population. PMID: 24606907
    39. This is probably the first report of identification of a significantly low proportion of missense PAH mutations from PKU families and together with the presence of a high proportion of splice, insertion-deletion, and nonsense mutations. PMID: 24130151
    40. Analysis of the published data shows similar percentage of the "BH4-responsive" variants of a PAH gene in patients from other countries of Eastern Europe PMID: 24350308
    41. Twenty phenylalanine hydroxylase gene mutations were discovered. PMID: 24510552
    42. A total of 98 mutations were detected in 110 phenylalanine hydroxylase alleles. PMID: 24510568
    43. 125 new mutations were found in exons 6, 7 and 12 of PAH in patients with hyperphenylalaninemia. PMID: 24078561
    44. Genotype-phenotype correlation of PAH gene mutations in phenylketonuria in a Syrian population. PMID: 23856132
    45. The observed phenotype is not always consistent with genotype predicting effect in Chinese phenylalanine hydroxylase deficiency patients. PMID: 23932990
    46. The five most prevalent PAH mutations found in patients were p.R408W, IVS12 + 1G>A, p.R261Q, p.R158Q and IVS2 + 5G>C. PMID: 22526846
    47. A new model for allosteric regulation of phenylalanine hydroxylase: implications for disease and therapeutics. PMID: 23296088
    48. Thirteen different mutations were identified in the PAH gene in Lebanese patients with phenylalanine hydroxylase deficiency. PMID: 23220018
    49. The p.G352fsdelG mutation in the PAH gene does not appear to be prevalent in the Moroccan population and would be responsible for only few cases of phenylketonuria. PMID: 22808937
    50. PAH exon 11 is vulnerable due to a weak 3' splice site. PMID: 22698810

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  • 相关疾病：
    Phenylketonuria (PKU); Non-phenylketonuria hyperphenylalaninemia (Non-PKU HPA); Hyperphenylalaninemia (HPA)
  • 蛋白家族：
    Biopterin-dependent aromatic amino acid hydroxylase family
  • 数据库链接：

    HGNC: 8582

    OMIM: 261600

    KEGG: hsa:5053

    STRING: 9606.ENSP00000448059

    UniGene: Hs.560019