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  4. Recombinant Human Origin recognition complex subunit 2 (ORC2)

Recombinant Human Origin recognition complex subunit 2 (ORC2)

  • 货号：
    CSB-YP614412HU
  • 规格：
  • 来源：
    Yeast
  • 其他：
  • 货号：
    CSB-EP614412HU
  • 规格：
  • 来源：
    E.coli
  • 其他：
  • 货号：
    CSB-EP614412HU-B
  • 规格：
  • 来源：
    E.coli
  • 共轭：
    Avi-tag Biotinylated

    E. coli biotin ligase (BirA) is highly specific in covalently attaching biotin to the 15 amino acid AviTag peptide. This recombinant protein was biotinylated in vivo by AviTag-BirA technology, which method is BriA catalyzes amide linkage between the biotin and the specific lysine of the AviTag.

  • 其他：
  • 货号：
    CSB-BP614412HU
  • 规格：
  • 来源：
    Baculovirus
  • 其他：
  • 货号：
    CSB-MP614412HU
  • 规格：
  • 来源：
    Mammalian cell
  • 其他：

产品详情

  • 纯度：
    >85% (SDS-PAGE)
  • 基因名：
  • Uniprot No.：
  • 别名：
    orc2; ORC2_HUMAN; ORC2L; Origin of replication 2 (yeast homolog) like; Origin recognition complex protein 2 homolog; Origin recognition complex subunit 2; Origin recognition complex subunit 2 isoform B; Origin recognition complex subunit 2 like (yeast); Origin recognition complex, subunit 2 homolog; Origin recognition complex, subunit 2, S. cerevisiae, homolog of; Origin recognition complex, subunit 2-like (S. cerevisiae)
  • 种属：
    Homo sapiens (Human)
  • 蛋白长度：
    full length protein
  • 表达区域：
    1-577
  • 氨基酸序列
    MSKPELKEDK MLEVHFVGDD DVLNHILDRE GGAKLKKERA QLLVNPKKII KKPEYDLEED DQEVLKDQNY VEIMGRDVQE SLKNGSATGG GNKVYSFQNR KHSEKMAKLA SELAKTPQKS VSFSLKNDPE ITINVPQSSK GHSASDKVQP KNNDKSEFLS TAPRSLRKRL IVPRSHSDSE SEYSASNSED DEGVAQEHEE DTNAVIFSQK IQAQNRVVSA PVGKETPSKR MKRDKTSDLV EEYFEAHSSS KVLTSDRTLQ KLKRAKLDQQ TLRNLLSKVS PSFSAELKQL NQQYEKLFHK WMLQLHLGFN IVLYGLGSKR DLLERFRTTM LQDSIHVVIN GFFPGISVKS VLNSITEEVL DHMGTFRSIL DQLDWIVNKF KEDSSLELFL LIHNLDSQML RGEKSQQIIG QLSSLHNIYL IASIDHLNAP LMWDHAKQSL FNWLWYETTT YSPYTEETSY ENSLLVKQSG SLPLSSLTHV LRSLTPNARG IFRLLIKYQL DNQDNPSYIG LSFQDFYQQC REAFLVNSDL TLRAQLTEFR DHKLIRTKKG TDGVEYLLIP VDNGTLTDFL EKEEEEA
  • 蛋白标签：
    Tag type will be determined during the manufacturing process.
    The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.
  • 产品提供形式：
    Lyophilized powder
    Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
  • 复溶：
    We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
  • 储存条件：
    Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
  • 保质期：
    The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
    Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
  • 货期：
    Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
    Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
  • 注意事项：
    Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
  • Datasheet ：
    Please contact us to get it.

产品评价

靶点详情

  • 功能：
    Component of the origin recognition complex (ORC) that binds origins of replication. DNA-binding is ATP-dependent. The specific DNA sequences that define origins of replication have not been identified yet. ORC is required to assemble the pre-replication complex necessary to initiate DNA replication. Binds histone H3 and H4 trimethylation marks H3K9me3, H3K20me3 and H4K27me3. Stabilizes LRWD1, by protecting it from ubiquitin-mediated proteasomal degradation. Also stabilizes ORC3.
  • 基因功能参考文献：
    1. the ORC2 complex with E2 restricts viral replication in the maintenance phase of the viral replication program PMID: 27701460
    2. ORC2 SUMOylation promotes recruitment of KDM5A to centromeres. PMID: 27052177
    3. Protein phosphatase 1 dephosphorylates Orc2 to promote the binding of ORC to chromatin and replication origins for the subsequent round of the cell cycle. PMID: 24792176
    4. These results suggest that PP1 dephosphorylates Orc2 to promote the binding of ORC to chromatin. PMID: 24732362
    5. Gemcitabine resistance is dependent on Plk1-mediated phosphorylation of Orc2 and Hbo1. PMID: 23188630
    6. Orc2 protects ORCA from ubiquitin-mediated degradation in vivo. PMID: 22935713
    7. The phosphorylation of ORC2 dissociates origin recognition complex (ORC) from chromatin and replication origins and inhibits binding of ORC to newly replicated DNA. PMID: 22334659
    8. Plk1 phosphorylation of Orc2 promotes DNA replication under conditions of stress PMID: 21947279
    9. the human origin recognition complex has a role in sequence-independent DNA binding and replication initiation PMID: 12897055
    10. The precise nucleotide of binding for ORC2 was identified near the stgart site for leading-strand synthesis; the transition to the post-replicative complex is accompanied by a 17 bp displacement of ORC2 towards the start site. PMID: 12912926
    11. Study using HCT116 haplo-insufficient cells and Orc2 hypomorphic cells demonstrates that human Ku80 and Orc2 bind to replication origins independently of each other. PMID: 15910003
    12. The low level of Orc2 in Orc2 hypomorphic cells hinders normal cell cycle progression by delaying the activation of G1 cyclin-dependent kinases. PMID: 16407251
    13. human Orc2 has specific domains that are required in vivo for assembly and nuclear localization of the origin recognition complex PMID: 16762929

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  • 亚细胞定位：
    Nucleus.
  • 蛋白家族：
    ORC2 family
  • 数据库链接：

    HGNC: 8488

    OMIM: 601182

    KEGG: hsa:4999

    STRING: 9606.ENSP00000234296

    UniGene: Hs.444870