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  4. Recombinant Human N-acyl-phosphatidylethanolamine-hydrolyzing phospholipase D (NAPEPLD)

Recombinant Human N-acyl-phosphatidylethanolamine-hydrolyzing phospholipase D (NAPEPLD)

  • 货号：
    CSB-YP764247HU
  • 规格：
  • 来源：
    Yeast
  • 其他：
  • 货号：
    CSB-EP764247HU
  • 规格：
  • 来源：
    E.coli
  • 其他：
  • 货号：
    CSB-EP764247HU-B
  • 规格：
  • 来源：
    E.coli
  • 共轭：
    Avi-tag Biotinylated

    E. coli biotin ligase (BirA) is highly specific in covalently attaching biotin to the 15 amino acid AviTag peptide. This recombinant protein was biotinylated in vivo by AviTag-BirA technology, which method is BriA catalyzes amide linkage between the biotin and the specific lysine of the AviTag.

  • 其他：
  • 货号：
    CSB-BP764247HU
  • 规格：
  • 来源：
    Baculovirus
  • 其他：
  • 货号：
    CSB-MP764247HU
  • 规格：
  • 来源：
    Mammalian cell
  • 其他：

产品详情

  • 纯度：
    >85% (SDS-PAGE)
  • 基因名：
    NAPEPLD
  • Uniprot No.：
  • 别名：
    C7orf18; DKFZp781D1098; FMP30 ; Mbldc1; N acyl phosphatidylethanolamine hydrolyzing phospholipase D; N acyl phosphatidylethanolamine phospholipase D; N-acyl phosphatidylethanolamine phospholipase D; N-acyl-phosphatidylethanolamine-hydrolyzing phospholipase D; NAPE hydrolyzing phospholipase D; NAPE-hydrolyzing phospholipase D; NAPE-PLD; NAPEP_HUMAN; NAPEPLD
  • 种属：
    Homo sapiens (Human)
  • 蛋白长度：
    full length protein
  • 表达区域：
    1-393
  • 氨基酸序列
    MDENESNQSL MTSSQYPKEA VRKRQNSARN SGASDSSRFS RKSFKLDYRL EEDVTKSKKG KDGRFVNPWP TWKNPSIPNV LRWLIMEKDH SSVPSSKEEL DKELPVLKPY FITNPEEAGV REAGLRVTWL GHATVMVEMD ELIFLTDPIF SSRASPSQYM GPKRFRRSPC TISELPPIDA VLISHNHYDH LDYNSVIALN ERFGNELRWF VPLGLLDWMQ KCGCENVIEL DWWEENCVPG HDKVTFVFTP SQHWCKRTLM DDNKVLWGSW SVLGPWNRFF FAGDTGYCPA FEEIGKRFGP FDLAAIPIGA YEPRWFMKYQ HVDPEEAVRI HTDVQTKKSM AIHWGTFALA NEHYLEPPVK LNEALERYGL NAEDFFVLKH GESRYLNNDD ENF
  • 蛋白标签：
    Tag type will be determined during the manufacturing process.
    The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.
  • 产品提供形式：
    Lyophilized powder
    Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
  • 复溶：
    We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
  • 储存条件：
    Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
  • 保质期：
    The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
    Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
  • 货期：
    Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
    Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
  • 注意事项：
    Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
  • Datasheet ：
    Please contact us to get it.

产品评价

靶点详情

  • 功能：
    D-type phospholipase that hydrolyzes N-acyl-phosphatidylethanolamines (NAPEs) to produce bioactive N-acylethanolamines/fatty acid ethanolamides (NAEs/FAEs) and phosphatidic acid. Cleaves the terminal phosphodiester bond of diacyl- and alkenylacyl-NAPEs, primarily playing a role in the generation of long-chain saturated and monounsaturated NAEs in the brain. May control NAPE homeostasis in dopaminergic neuron membranes and regulate neuron survival, partly through RAC1 activation. As a regulator of lipid metabolism in the adipose tissue, mediates the crosstalk between adipocytes, gut microbiota and immune cells to control body temperature and weight. In particular, regulates energy homeostasis by promoting cold-induced brown or beige adipocyte differentiation program to generate heat from fatty acids and glucose. Has limited D-type phospholipase activity toward N-acyl lyso-NAPEs.
  • 基因功能参考文献：
    1. The AC genotype and C allele of NAPE-PLD rs12540583 locus are risk factors for schizophrenia. PMID: 29652995
    2. NAPE-PLD forms homodimers partly separated by an internal channel and uniquely adapted to associate with phospholipids. PMID: 25684574
    3. our results do not support a clear role of FAAH, CNR1 and NAPE-PLD in BD and lithium response. PMID: 24126189
    4. a major physiological role of NAPE-PLD PMID: 24018423
    5. The differential expression of NAPE-PLD and FAAH suggests that Anandamide could play an important role in the pathophysiology of preeclampsia. PMID: 23122699
    6. NAPE-PLD expression increase steadily after infancy, peaking in adulthood. PMID: 22827915
    7. The results of this study suggested that NAPEPLD was alterated in multip;e sclerosis. PMID: 21251115
    8. a common haplotype in NAPEPLD, an enzyme involved in endocannabinoid synthesis, was protective against obesity. PMID: 20885390
    9. Observational study of gene-disease association. (HuGE Navigator) PMID: 20885390
    10. During the menstrual cycle, NAPE-PLD immunoreactivity was down-regulated in the secretory epithelial gland compared to the proliferative epithelial gland and unaffected in the stroma. PMID: 20369362
    11. The human NAPE-PLD was activated by phosphatidylethanolamine and inhibited by the beta-lactamase substrate nitrocefin. PMID: 19715685

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  • 亚细胞定位：
    Golgi apparatus membrane; Peripheral membrane protein. Early endosome membrane; Peripheral membrane protein. Nucleus envelope. Nucleus, nucleoplasm.
  • 蛋白家族：
    NAPE-PLD family
  • 组织特异性：
    Widely expressed. Highest expression in brain, kidney and testis (at protein level). Expressed in adipose tissue (at protein level).
  • 数据库链接：

    HGNC: 21683

    OMIM: 612334

    KEGG: hsa:222236

    STRING: 9606.ENSP00000340093

    UniGene: Hs.324271