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  4. Recombinant Human Mitochondrial cardiolipin hydrolase (PLD6)

Recombinant Human Mitochondrial cardiolipin hydrolase (PLD6)

  • 货号：
    CSB-EP836649HUe1
  • 规格：
    ￥2832
  • 图片：
    • (Tris-Glycine gel) Discontinuous SDS-PAGE (reduced) with 5% enrichment gel and 15% separation gel.
  • 其他：

产品详情

  • 纯度：
    Greater than 85% as determined by SDS-PAGE.
  • 基因名：
    PLD6
  • Uniprot No.：
  • 别名：
    Choline phosphatase 6; Mitochondrial cardiolipin hydrolase; Mitochondrial phospholipase; MitoPLD; Phosphatidylcholine hydrolyzing phospholipase D6; Phosphatidylcholine-hydrolyzing phospholipase D6; Phospholipase D family member 6; Phospholipase D6; PLD 6; pld6; PLD6_HUMAN; Protein zucchini homolog; ZUC; Zucchini, Drosophila, homolog of
  • 种属：
    Homo sapiens (Human)
  • 蛋白长度：
    Full Length
  • 来源：
    E.coli
  • 分子量：
    28.3 kDa
  • 表达区域：
    1-252aa
  • 氨基酸序列
    MGRLSWQVAAAAAVGLALTLEALPWVLRWLRSRRRRPRREALFFPSQVTCTEALLRAPGAELAELPEGCPCGLPHGESALSRLLRALLAARASLDLCLFAFSSPQLGRAVQLLHQRGVRVRVVTDCDYMALNGSQIGLLRKAGIQVRHDQDPGYMHHKFAIVDKRVLITGSLNWTTQAIQNNRENVLITEDDEYVRLFLEEFERIWEQFNPTKYTFFPPKKSHGSCAPPVSRAGGRLLSWHRTCGTSSESQT
    Note: The complete sequence including tag sequence, target protein sequence and linker sequence could be provided upon request.
  • 蛋白标签：
    Tag-Free
  • 产品提供形式：
    Liquid or Lyophilized powder
    Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
  • 缓冲液：
    Tris-based buffer，50% glycerol
  • 储存条件：
    Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
  • 保质期：
    The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
    Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
  • 货期：
    Basically, we can dispatch the products out in 1-3 working days after receiving your orders. Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
    Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
  • 注意事项：
    Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
  • 产品描述：
    重组人线粒体心磷脂水解酶（PLD6）是一种在线粒体中表达的酶，其功能与膜脂代谢和线粒体DNA修复等生物过程有关。下面是重组人PLD6的一般步骤：
    设计和合成基因：基于人PLD6的氨基酸序列，设计并合成含有PLD6基因的DNA序列。这可以通过基因合成公司或PCR方法完成。
    克隆基因：将PLD6基因插入到表达载体中，这可以通过PCR扩增和限制性内切酶切割的方法完成。建议选择一些经过验证的表达载体，如pET系列载体，这些载体中包含了一些促进蛋白表达的元件，如启动子、增强子、终止子等。
    转染宿主细胞：在细胞中转染含有PLD6基因的表达载体。这些宿主细胞可以选择常见的哺乳动物细胞系，如CHO细胞、HEK293细胞等。
    培养和表达蛋白：根据表达载体中的启动子和调节元件，诱导PLD6蛋白在转染的宿主细胞中表达。在培养期间，需要添加抗生素等来保持表达载体的稳定。
    蛋白质的纯化：通过细胞破碎和离心等方法提取含有PLD6蛋白的细胞裂解液。然后使用亲和层析、离子交换层析、凝胶过滤层析等方法对蛋白进行纯化。
    蛋白质的鉴定和活性检测：使用SDS-PAGE、wb等方法对纯化后的PLD6蛋白进行鉴定和定量。此外，可以使用酶活性分析等方法来检测其生物学活性和功能。
    需要注意的是，线粒体蛋白的表达和纯化需要特殊的条件和方法。因此，在进行PLD6的表达和纯化时，需要注意线粒体蛋白的稳定性和功能性，避免蛋白质的降解和失活。
  • Datasheet & COA：
    Please contact us to get it.

产品评价

靶点详情

  • 功能：
    Presents phospholipase and nuclease activities, depending on the different physiological conditions. Interaction with Mitoguardin (MIGA1 or MIGA2) affects the dimer conformation, facilitating the lipase activity over the nuclease activity. Plays a key role in mitochondrial fusion and fission via its phospholipase activity. In its phospholipase role, it uses the mitochondrial lipid cardiolipin as substrate to generate phosphatidate (PA or 1,2-diacyl-sn-glycero-3-phosphate), a second messenger signaling lipid. Production of PA facilitates Mitofusin-mediated fusion, whereas the cleavage of PA by the Lipin family of phosphatases produces diacylgycerol (DAG) which promotes mitochondrial fission. Both Lipin and DAG regulate mitochondrial dynamics and membrane fusion/fission, important processes for adapting mitochondrial metabolism to changes in cell physiology. Mitochondrial fusion enables cells to cope with the increased nucleotide demand during DNA synthesis. Mitochondrial function and dynamics are closely associated with biological processes such as cell growth, proliferation, and differentiation. Mediator of MYC activity, promotes mitochondrial fusion and activates AMPK which in turn inhibits YAP/TAZ, thereby inducing cell growth and proliferation. The endonuclease activity plays a critical role in PIWI-interacting RNA (piRNA) biogenesis during spermatogenesis. Implicated in spermatogenesis and sperm fertility in testicular germ cells, its single strand-specific nuclease activity is critical for the biogenesis/maturation of PIWI-interacting RNA (piRNA). MOV10L1 selectively binds to piRNA precursors and funnels them to the endonuclease that catalyzes the first cleavage step of piRNA processing to generate piRNA intermediate fragments that are subsequently loaded to Piwi proteins. Cleaves either DNA or RNA substrates with similar affinity, producing a 5' phosphate end, in this way it participates in the processing of primary piRNA transcripts. piRNAs provide essential protection against the activity of mobile genetic elements. piRNA-mediated transposon silencing is thus critical for maintaining genome stability, in particular in germline cells when transposons are mobilized as a consequence of wide-spread genomic demethylation. PA may act as signaling molecule in the recognition/transport of the precursor RNAs of primary piRNAs. Interacts with tesmin in testes, suggesting a role in spermatogenesis via association with its interacting partner.
  • 基因功能参考文献：
    1. Propose that MIGA proteins promote mitochondrial fusion by regulating mitochondrial phospholipid metabolism via MitoPLD. PMID: 26711011
    2. MitoPLD performs a critical function in a pathway that creates a specialized form of RNAi required by developing spermatocytes to suppress transposon mobilization during meiosis. PMID: 22281006
    3. Data suggest that mitochondrial-surface phosphatidic acid generated by MitoPLD/Zuc recruits or activates nuage components critical for piRNA production. PMID: 21397848
    4. PLD6, aka MitoPLD, encodes an N-terminal mitochondrial localization sequence, localizes to the outer mitochondrial surface, generates phosphatidic acid, a signaling lipid, and triggers trans-mitochondrial association; involved in mitochondrial fusion. PMID: 17028579
  • 亚细胞定位：
    Mitochondrion outer membrane; Single-pass membrane protein. Golgi apparatus.
  • 蛋白家族：
    Phospholipase D family, MitoPLD/Zucchini subfamily
  • 组织特异性：
    Predominantly expressed in testis and ovary, but not limited to gonads (at protein level). It is also found in brain, heart, pituitary gland, prostate, pancreas, thyroid, bone marrow, lung and muscle.
  • 数据库链接：

    HGNC: 30447

    OMIM: 614960

    KEGG: hsa:201164

    STRING: 9606.ENSP00000317177

    UniGene: Hs.729079