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  4. Recombinant Human Inactive tyrosine-protein kinase 7 (PTK7), partial

Recombinant Human Inactive tyrosine-protein kinase 7 (PTK7), partial

In Stock
  • 货号：
    CSB-YP622651HU
  • 规格：
    ￥1500
  • 图片：
    • (Tris-Glycine gel) Discontinuous SDS-PAGE (reduced) with 5% enrichment gel and 15% separation gel.
    • Based on the SEQUEST from database of Yeast host and target protein, the LC-MS/MS Analysis result of CSB-YP622651HU could indicate that this peptide derived from Yeast-expressed Homo sapiens (Human) PTK7.
    • Based on the SEQUEST from database of Yeast host and target protein, the LC-MS/MS Analysis result of CSB-YP622651HU could indicate that this peptide derived from Yeast-expressed Homo sapiens (Human) PTK7.
  • 其他：

产品详情

  • 纯度：
    Greater than 90% as determined by SDS-PAGE.
  • 基因名：
  • Uniprot No.：
  • 别名：
    8430404F20Rik; CCK 4; CCK-4; Colon carcinoma kinase 4; Human colon carcinoma kinase 4 (CCK4) mRNA complete cds; Inactive tyrosine-protein kinase 7; mPTK7/CCK4; OTTHUMP00000217016; Protein tyrosine kinase PTK 7; Protein-tyrosine kinase 7; Pseudo tyrosine kinase receptor 7; PTK 7; PTK7; PTK7 protein tyrosine kinase 7; PTK7 protein tyrosine kinase; PTK7_HUMAN; Serum response factor; Srf; Tyrosine-protein kinase-like 7
  • 种属：
    Homo sapiens (Human)
  • 蛋白长度：
    Extracellular Domain
  • 来源：
    Yeast
  • 分子量：
    76.6kDa
  • 表达区域：
    31-704aa
  • 氨基酸序列
    AIVFIKQPSSQDALQGRRALLRCEVEAPGPVHVYWLLDGAPVQDTERRFAQGSSLSFAAVDRLQDSGTFQCVARDDVTGEEARSANASFNIKWIEAGPVVLKHPASEAEIQPQTQVTLRCHIDGHPRPTYQWFRDGTPLSDGQSNHTVSSKERNLTLRPAGPEHSGLYSCCAHSAFGQACSSQNFTLSIADESFARVVLAPQDVVVARYEEAMFHCQFSAQPPPSLQWLFEDETPITNRSRPPHLRRATVFANGSLLLTQVRPRNAGIYRCIGQGQRGPPIILEATLHLAEIEDMPLFEPRVFTAGSEERVTCLPPKGLPEPSVWWEHAGVRLPTHGRVYQKGHELVLANIAESDAGVYTCHAANLAGQRRQDVNITVATVPSWLKKPQDSQLEEGKPGYLDCLTQATPKPTVVWYRNQMLISEDSRFEVFKNGTLRINSVEVYDGTWYRCMSSTPAGSIEAQARVQVLEKLKFTPPPQPQQCMEFDKEATVPCSATGREKPTIKWERADGSSLPEWVTDNAGTLHFARVTRDDAGNYTCIASNGPQGQIRAHVQLTVAVFITFKVEPERTTVYQGHTALLQCEAQGDPKPLIQWKGKDRILDPTKLGPRMHIFQNGSLVIHDVAPEDSGRYTCIAGNSCNIKHTEAPLYVVDKPVPEESEGPGSPPPYKMIQT
    Note: The complete sequence including tag sequence, target protein sequence and linker sequence could be provided upon request.
  • 蛋白标签：
    N-terminal 6xHis-tagged
  • 产品提供形式：
    Liquid or Lyophilized powder
    Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
  • 缓冲液：
    Tris-based buffer,50% glycerol
  • 储存条件：
    Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
  • 保质期：
    The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
    Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
  • 货期：
    3-7 business days
  • 注意事项：
    Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
  • 产品描述：

    The synthesis of this Recombinant Human PTK7 protein depends on the utilization of recombinant DNA technology to a large degree. DNA sequences that encoded the PTK7 protein could be inserted into a vector and introduced into an expression host, Yeast, where it could be easily expressed in and purified from. The expression of this PTK7 protein was at 31-704aa. N-terminal 6xHis tag was fused with this protein. The purity is 90%+ determined by SDS-PAGE.

    PTK7 (also known as CCK4) is gene encoding a protein named inactive tyrosine-protein kinase 7(PTK7) in human. The protein encoded by this gene is also known as colon carcinoma kinase 4 (CCK4), protein-tyrosine kinase 7, pseudo tyrosine kinase receptor 7 and tyrosine-protein kinase-like 7. The protein encoded by this gene is a positive regulator of canonical Wnt signaling pathway with its coreceptor activity. Moreover, this protein is also involved other biological processes, such as actin cytoskeleton reorganization, cell adhesion, cell migration and signal transduction.

  • Datasheet & COA：
    Please contact us to get it.

产品评价

靶点详情

  • 功能：
    Inactive tyrosine kinase involved in Wnt signaling pathway. Component of both the non-canonical (also known as the Wnt/planar cell polarity signaling) and the canonical Wnt signaling pathway. Functions in cell adhesion, cell migration, cell polarity, proliferation, actin cytoskeleton reorganization and apoptosis. Has a role in embryogenesis, epithelial tissue organization and angiogenesis.
  • 基因功能参考文献：
    1. miR2055p is involved in the proliferation, migration and invasion of colorectal cancer cells through inhibiting PTK7. PMID: 29488611
    2. Higher expression of PTK7 significantly indicates worse prognosis in human malignancies PMID: 28924970
    3. Inhibition of PTK7 by siRNA treatment significantly decreases the viability of atypical teratoid rhabdoid tumors (ATRT)patient-derived tumor cell lines.These studies provide the groundwork for future preclinical in vivo studies aiming to investigate the efficacy of PTK7 inhibition on ATRT tumor growth PMID: 28442586
    4. PTK7 localization and protein stability is affected by canonical Wnt ligands. PMID: 28420671
    5. These findings demonstrate that PTK7 upregulates MMP9 through activation of AP-1 and NF-kappaB and, thus increases invasive properties of ESCC cells. PMID: 27689325
    6. PTK7 overexpression is associated with esophageal squamous cell carcinoma. PMID: 28545451
    7. Our data indicate that PTK7 protein expression is associated with the prognosis of oral tongue squamous cell carcinoma PMID: 28547980
    8. PTK7 expression was correlated with tumor differentiation (P=0.027), lymph node metastasis (P=0.005), distant metastasis (P=0.001) and TNM stage (P=0.028) of colorectal cancer patients PMID: 27499181
    9. High PTK7 expression is associated with radioresistance in esophageal squamous cell carcinoma. PMID: 27557627
    10. PTK7 enriches in self-renewing, multipotent stem cells of the human colon. PMID: 26549850
    11. findings implicate PTK7 as a risk factor for NTDs and provide additional evidence for a pathogenic role of PCP signaling in these malformations PMID: 26368655
    12. overexpression in PTK7-negative cancer cells led to increased metastatic events. PTK7 expression thus represents a potential prognostic biomarker and a novel therapeutic target in CRC. PMID: 25962058
    13. data demonstrates that PTK7 regulates the activity of KDR biphasically by inducing oligomerization of KDR molecules at lower concentrations and by surrounding KDR molecules at higher concentrations. PMID: 25986862
    14. PTK7 regulates Id1 expression in CD44-high glioma cell lines. Targeting PTK7 could be an effective strategy for treating glioma with high CD44 expression. PMID: 25204555
    15. The results suggest that N-acylethanolamine acid amidase and protein tyrosine kinase 7 may be used as potential tissue biomarkers to avoid overtreatment of non-aggressive prostate cancer PMID: 24741114
    16. Expression level of PTK7 was significantly decreased from benign to malignant ovarian epithelial tumors. survival analysis showed that patients with negative expression of PTK7 protein had poorer outcome than those with positive expression. PMID: 25550828
    17. PTK7 acts as a cancer-related gene and may be a potent prognostic marker for Hepatocellular carcinoma(HCC). PMID: 25796105
    18. results provide convincing evidence that both PTK7 expression and proteolysis, rather than the level of the cellular full-length PTK7 alone, contribute to efficient directional cell motility and metastasis in cancer PMID: 25006253
    19. PTK7 expression plays an important role in the invasiveness of intrahepatic cholangiocarcinoma (ICC) cells and leads to a poor prognosis in ICC patients. PMID: 24587299
    20. PTK 7 is a transforming gene and prognostic marker for breast cancer and nodal metastasis involvement. PMID: 24409301
    21. This work defines PTK7 as a highly and specifically expressed gene in adenocarcinoma and a potential therapeutic target in this subset of NSCLC. PMID: 24654231
    22. Increased expression of PTK7 was inversely correlated with overall survival. PMID: 23663482
    23. in patients treated by chemotherapy, patients with PTK7-negative tumors seemed to have better DFS than those with PTK7-positive tumors, particularly for patients treated with only anthracycline-therapy drugs PMID: 24023307
    24. PTK7 is a novel marker of epithelial to mesenchymal transitions PMID: 23209741
    25. cleavage of the PTK7 ectodomain by an ADAM proteinase was coupled with the membrane type-1 matrix metalloproteinase (MT1-MMP) cleavage of the PKP(621) downward arrowLI site in the seventh Ig-like domain of PTK7. PMID: 23095747
    26. PTK7 has utility as a biomarker for detecting minimal residual disease of T cell acute lymphoblastic leukemia in the bone marrow PMID: 22898210
    27. a novel role for PTK7 in the tumorigenesis via generation of PTK7-CTF2 by sequential cleavage of ADAM17 and gamma-secretase. PMID: 22665490
    28. aberrations in the membrane type 1 matrix metalloproteinase/PTK7 axis are detrimental to cell movements that shape the body plan PMID: 21518755
    29. Observations may indicate a role for PTK7 in cell proliferation and cell apoptosis and may provide a potential therapeutic pathway for the treatment of a variety of cancers. PMID: 21103379
    30. Protein Tyrosine Kinase 7 is required for beta-catenin-dependent transcriptional events induced by canonical Wnt ligands PMID: 21132015
    31. Results indicate that PTK7 is a major target of MT1-MMP. PMID: 20837484
    32. PTK7 is a planar cell polarity component expressed in the myeloid progenitor compartment that conveys promigratory and antiapoptotic signals into the cell and represents an independent prognosis factor in patients treated with induction chemotherapy. PMID: 20558616
    33. cloning, sequencing and expression of gene and its splice variants PMID: 12427550
    34. Lack of a thermodynamically meaningful self-association propensity for the CCK4 transmembrane domains demonstrates that the conserved GxxxG motif is not sufficient to drive CCK4 transmembrane helix-helix interactions. PMID: 15683231
    35. mechanism of PTK7 upregulation in colorectal cancer PMID: 17671748
    36. PTK7 plays an important role not only in tube formation, migration, and invasion of endothelial cells but also in angiogenesis. PMID: 18471990

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  • 亚细胞定位：
    Membrane; Single-pass type I membrane protein. Cell junction. Note=Colocalizes with MMP14 at cell junctions. Also localizes at the leading edge of migrating cells.
  • 蛋白家族：
    Protein kinase superfamily, Tyr protein kinase family, Insulin receptor subfamily
  • 组织特异性：
    Highly expressed in lung, liver, pancreas, kidney, placenta and melanocytes. Weakly expressed in thyroid gland, ovary, brain, heart and skeletal muscle. Also expressed in erythroleukemia cells. But not expressed in colon.
  • 数据库链接：

    HGNC: 9618

    OMIM: 601890

    KEGG: hsa:5754

    STRING: 9606.ENSP00000230419

    UniGene: Hs.90572