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  4. Recombinant Human Importin subunit alpha-3 (KPNA4)

Recombinant Human Importin subunit alpha-3 (KPNA4)

  • 货号：
    CSB-YP012486HU
  • 规格：
  • 来源：
    Yeast
  • 其他：
  • 货号：
    CSB-EP012486HU
  • 规格：
  • 来源：
    E.coli
  • 其他：
  • 货号：
    CSB-EP012486HU-B
  • 规格：
  • 来源：
    E.coli
  • 共轭：
    Avi-tag Biotinylated

    E. coli biotin ligase (BirA) is highly specific in covalently attaching biotin to the 15 amino acid AviTag peptide. This recombinant protein was biotinylated in vivo by AviTag-BirA technology, which method is BriA catalyzes amide linkage between the biotin and the specific lysine of the AviTag.

  • 其他：
  • 货号：
    CSB-BP012486HU
  • 规格：
  • 来源：
    Baculovirus
  • 其他：
  • 货号：
    CSB-MP012486HU
  • 规格：
  • 来源：
    Mammalian cell
  • 其他：

产品详情

  • 纯度：
    >85% (SDS-PAGE)
  • 基因名：
    KPNA4
  • Uniprot No.：
  • 别名：
    FLJ31113; IMA3_HUMAN; Importin alpha 3; Importin alpha 4 subunit; Importin alpha Q1; Importin subunit alpha 3; Importin subunit alpha-3; IPO A3; IPOA 3; IPOA3; Karyopherin alpha 4 (importin alpha 3); Karyopherin alpha 4; Karyopherin alpha 4 importin alpha 3; Karyopherin alpha 4 subunit; Karyopherin subunit alpha 4 ; Karyopherin subunit alpha-4; KPNA 4; Kpna4; MGC12217; MGC26703; OTTHUMP00000213880; QIP 1; Qip1; Qip1 protein; SRP 3; SRP3
  • 种属：
    Homo sapiens (Human)
  • 蛋白长度：
    Full Length of Mature Protein
  • 表达区域：
    2-521
  • 氨基酸序列
    ADNEKLDNQ RLKNFKNKGR DLETMRRQRN EVVVELRKNK RDEHLLKRRN VPHEDICEDS DIDGDYRVQN TSLEAIVQNA SSDNQGIQLS AVQAARKLLS SDRNPPIDDL IKSGILPILV HCLERDDNPS LQFEAAWALT NIASGTSEQT QAVVQSNAVP LFLRLLHSPH QNVCEQAVWA LGNIIGDGPQ CRDYVISLGV VKPLLSFISP SIPITFLRNV TWVMVNLCRH KDPPPPMETI QEILPALCVL IHHTDVNILV DTVWALSYLT DAGNEQIQMV IDSGIVPHLV PLLSHQEVKV QTAALRAVGN IVTGTDEQTQ VVLNCDALSH FPALLTHPKE KINKEAVWFL SNITAGNQQQ VQAVIDANLV PMIIHLLDKG DFGTQKEAAW AISNLTISGR KDQVAYLIQQ NVIPPFCNLL TVKDAQVVQV VLDGLSNILK MAEDEAETIG NLIEECGGLE KIEQLQNHEN EDIYKLAYEI IDQFFSSDDI DEDPSLVPEA IQGGTFGFNS SANVPTEGFQ F
  • 蛋白标签：
    Tag type will be determined during the manufacturing process.
    The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.
  • 产品提供形式：
    Lyophilized powder
    Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
  • 复溶：
    We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
  • 储存条件：
    Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
  • 保质期：
    The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
    Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
  • 货期：
    Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
    Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
  • 注意事项：
    Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
  • Datasheet ：
    Please contact us to get it.

产品评价

靶点详情

  • 功能：
    Functions in nuclear protein import as an adapter protein for nuclear receptor KPNB1. Binds specifically and directly to substrates containing either a simple or bipartite NLS motif. Docking of the importin/substrate complex to the nuclear pore complex (NPC) is mediated by KPNB1 through binding to nucleoporin FxFG repeats and the complex is subsequently translocated through the pore by an energy requiring, Ran-dependent mechanism. At the nucleoplasmic side of the NPC, Ran binds to importin-beta and the three components separate and importin-alpha and -beta are re-exported from the nucleus to the cytoplasm where GTP hydrolysis releases Ran from importin. The directionality of nuclear import is thought to be conferred by an asymmetric distribution of the GTP- and GDP-bound forms of Ran between the cytoplasm and nucleus. In vitro, mediates the nuclear import of human cytomegalovirus UL84 by recognizing a non-classical NLS. In vitro, mediates the nuclear import of human cytomegalovirus UL84 by recognizing a non-classical NLS.
  • 基因功能参考文献：
    1. Importin alpha3 facilitates the nuclear transport of the Ran guanine nucleotide exchange factor RCC1. PMID: 29042532
    2. Our results suggest a potential role of Hsa-miR-567 as a novel prognostic biomarker for BC and as regulator of KPNA4. PMID: 28000015
    3. Our results showed that miR-181 family expression was closely correlated with TCGA subtypes and patients' overall survival, indicating that miR-181b. PMID: 26283154
    4. upon DNA damage transport adaptor importin-alpha4 imports XPA into the nucleus in an ATR-dependent manner. PMID: 23861882
    5. Importin alpha3/Qip1 is involved in multiplication of mutant influenza virus with alanine mutation at amino acid 9 independently of nuclear transport function. PMID: 23383277
    6. Impalpha3 is an HIV integrase-interacting cofactor and is required for efficient HIV-1 nuclear import and replication. PMID: 20554775
    7. stress-induced accumulation of non-ubiquitinated p53 in the cytoplasm promotes interaction with importin-alpha3 and rapid import PMID: 19927155
    8. Expression of importin alpha3, a protein involved in p50/p65 nuclear import, was decreased in cells overexpressing PHB. PMID: 19710421
    9. Our results indicate that the nuclear import of RHA is mediated by the importin-alpha3/importin-beta-dependent pathway and suggest that the specificity for importin may regulate the functions of cargo proteins. PMID: 16375861
    10. Daxx interacts selectively with importin alpha3 through its NLS1 and NLS2 sequences. Conversely, importin alpha3 utilizes two NLS-binding sites for Daxx interaction, suggesting that it mediates nuclear import of Daxx. PMID: 17661348

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  • 亚细胞定位：
    Cytoplasm. Nucleus.
  • 蛋白家族：
    Importin alpha family
  • 组织特异性：
    Highly expressed in testis, ovary, small intestine, heart, skeletal muscle, lung and pancreas, but barely detectable in kidney, thymus, colon and peripheral blood leukocytes.
  • 数据库链接：

    HGNC: 6397

    OMIM: 602970

    KEGG: hsa:3840

    STRING: 9606.ENSP00000334373

    UniGene: Hs.467866