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  4. Recombinant Human High mobility group nucleosome-binding domain-containing protein 5 (HMGN5)

Recombinant Human High mobility group nucleosome-binding domain-containing protein 5 (HMGN5)

  • 货号：
    CSB-YP016099HU
  • 规格：
  • 来源：
    Yeast
  • 其他：
  • 货号：
    CSB-EP016099HU
  • 规格：
  • 来源：
    E.coli
  • 其他：
  • 货号：
    CSB-EP016099HU-B
  • 规格：
  • 来源：
    E.coli
  • 共轭：
    Avi-tag Biotinylated

    E. coli biotin ligase (BirA) is highly specific in covalently attaching biotin to the 15 amino acid AviTag peptide. This recombinant protein was biotinylated in vivo by AviTag-BirA technology, which method is BriA catalyzes amide linkage between the biotin and the specific lysine of the AviTag.

  • 其他：
  • 货号：
    CSB-BP016099HU
  • 规格：
  • 来源：
    Baculovirus
  • 其他：
  • 货号：
    CSB-MP016099HU
  • 规格：
  • 来源：
    Mammalian cell
  • 其他：

产品详情

  • 纯度：
    >85% (SDS-PAGE)
  • 基因名：
    HMGN5
  • Uniprot No.：
  • 别名：
    High mobility group nucleosome binding domain 5; High mobility group nucleosome-binding domain-containing protein 5; Hmgn5; HMGN5_HUMAN; NBP 45; NSBP 1; NSBP1; Nucleosomal binding protein 1; Nucleosome binding protein 1; Nucleosome-binding protein 1
  • 种属：
    Homo sapiens (Human)
  • 蛋白长度：
    Full length protein
  • 表达区域：
    1-282
  • 氨基酸序列
    MPKRKAAGQG DMRQEPKRRS ARLSAMLVPV TPEVKPKRTS SSRKMKTKSD MMEENIDTSA QAVAETKQEA VVEEDYNENA KNGEAKITEA PASEKEIVEV KEENIEDATE KGGEKKEAVA AEVKNEEEDQ KEDEEDQNEE KGEAGKEDKD EKGEEDGKED KNGNEKGEDA KEKEDGKKGE DGKGNGEDGK EKGEDEKEEE DRKETGDGKE NEDGKEKGDK KEGKDVKVKE DEKEREDGKE DEGGNEEEAG KEKEDLKEEE EGKEEDEIKE DDGKKEEPQS IV
  • 蛋白标签：
    Tag type will be determined during the manufacturing process.
    The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.
  • 产品提供形式：
    Lyophilized powder
    Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
  • 复溶：
    We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
  • 储存条件：
    Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
  • 保质期：
    The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
    Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
  • 货期：
    Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
    Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
  • 注意事项：
    Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
  • Datasheet ：
    Please contact us to get it.

产品评价

靶点详情

  • 功能：
    Preferentially binds to euchromatin and modulates cellular transcription by counteracting linker histone-mediated chromatin compaction.
  • 基因功能参考文献：
    1. Findings suggest that a miR-140-5p/HMGN5/autophagy regulatory loop plays a critical role in chemoresistance in osteosarcoma. In conclusion, our data elucidated that miR-140-5p promoted autophagy mediated by HMGN5 and sensitized osteosarcoma cells to chemotherapy. PMID: 28341864
    2. High HMGN5 expression is associated with osteosarcoma invasion. PMID: 28627703
    3. We observed decreased miR-409-3p expression in glioma, and overexpression of miR-409-3p repressed glioma cell invasion and proliferation by targeting HMGN5, an oncogene associated with glioma PMID: 28109076
    4. HMGN5 regulated the expression of multidrug resistance 1, cyclin B1, and Bcl-2. PMID: 28914995
    5. NSBP1 was highly expressed in NSCLC cells. NSBP1 siRNA knockdown suppressed NSCLC cell proliferation and invasion. miR326 has a putative binding site in the NSBP1 3'UTR. NSBP1 overexpression abolished miR326 inhibition of cyclin B1 and MMP9 expression. PMID: 26548724
    6. HMGN5 overexpression is correlated with advanced pathological grade and poorer prognosis in meningiomas PMID: 26315299
    7. Decreased miR-340 expression may contribute to the development and progression of prostate cancer through a mechanism that involves HMGN5. PMID: 26394192
    8. Our data first time identified miR-186 as the upstream regulator of NSBP1 PMID: 26290438
    9. High HMGN5 expression is associated with urothelial bladder cancer. PMID: 25796505
    10. HMGN5 is an oncogene and plays an important role in prostate cancer tumorigenesis and progression. the level of HMGN5 may be used as a biomarker to predict the patients that would benefit from gemcitabine treatment. PMID: 25572120
    11. HMGN5 plays an oncogenic role in human breast cancer by inhibiting cell proliferation and invasion, and activating apoptosis, which could be exploited as a target for therapy in human breast cancer. PMID: 25315189
    12. HMGN5 knockdown sensitizes prostate cancer cells to ionizing radiation PMID: 25307178
    13. HMGN5 plays oncogenic role in osteosarcoma by promoting cell proliferation and invasion, and could be exploited as a target for therapy in osteosarcoma. PMID: 24687550
    14. HMGN5 is a critical factor in the development of chemoresistance through regulating autophagy, and it offers a novel target for improving osteosarcoma therapy. PMID: 24664583
    15. Data indicate that cells lacking either high mobility group protein N5 (HMGN5) and lamina-associated polypeptide 2alpha (LAP2alpha) showed that loss of either protein affects the genome-wide distribution of the remaining partner. PMID: 23673662
    16. HMGN5 silencing to significantly inhibit A549 and H1299 cell proliferation. PMID: 22994738
    17. HMGN5 may be a potential molecular target with therapeutic relevance for the treatment of prostate cancer. PMID: 22504871
    18. NSBP1 plays oncogenic role in clear cell renal cell carcinoma (ccRCC) by promoting cell proliferation and invasion. PMID: 22420896
    19. The data of study suggested that HMGN5 may play a critical role in the development of gliomas. PMID: 21373965
    20. these results suggest that NSBP1 promotes the viability of bladder cancer cells through increased cell proliferation PMID: 21695596
    21. HMGN5 has a highly disordered structure, binds dynamically to nucleosome core particles, modulates the binding of H1 to chromatin, reduces the compaction of the chromatin fiber, and affects transcription. PMID: 21518955
    22. A positive correlation was observed between NSBP1 expression and cell proliferation and apoptosis in DU145 cells. PMID: 20531280
    23. The structure of the HMGN5 gene and the known properties of the HMGN5 protein, are described. PMID: 20123071

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  • 亚细胞定位：
    Nucleus.
  • 蛋白家族：
    HMGN family
  • 组织特异性：
    Ubiquitously expressed.
  • 数据库链接：

    HGNC: 8013

    OMIM: 300385

    KEGG: hsa:79366

    STRING: 9606.ENSP00000350848

    UniGene: Hs.282204