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  4. Recombinant Human Guanine nucleotide-binding protein G (i) subunit alpha-2 (GNAI2)

Recombinant Human Guanine nucleotide-binding protein G (i) subunit alpha-2 (GNAI2)

  • 货号：
    CSB-YP009589HU
  • 规格：
  • 来源：
    Yeast
  • 其他：
  • 货号：
    CSB-EP009589HU
  • 规格：
  • 来源：
    E.coli
  • 其他：
  • 货号：
    CSB-EP009589HU-B
  • 规格：
  • 来源：
    E.coli
  • 共轭：
    Avi-tag Biotinylated

    E. coli biotin ligase (BirA) is highly specific in covalently attaching biotin to the 15 amino acid AviTag peptide. This recombinant protein was biotinylated in vivo by AviTag-BirA technology, which method is BriA catalyzes amide linkage between the biotin and the specific lysine of the AviTag.

  • 其他：
  • 货号：
    CSB-BP009589HU
  • 规格：
  • 来源：
    Baculovirus
  • 其他：
  • 货号：
    CSB-MP009589HU
  • 规格：
  • 来源：
    Mammalian cell
  • 其他：

产品详情

  • 纯度：
    >85% (SDS-PAGE)
  • 基因名：
  • Uniprot No.：
  • 别名：
    Adenylate cyclase inhibiting G alpha protein ; Adenylate cyclase-inhibiting G alpha protein; GIP; GNAI 2; Gnai2; GNAI2_HUMAN; GNAI2B; GTP binding regulatory protein Gi alpha 2 chain; Guanine nucleotide binding protein G protein alpha inhibiting activity polypeptide 2 ; Guanine nucleotide-binding protein G(i) subunit alpha-2; H LUCA15.1; H LUCA16.1; WUGSC:H LUCA15.1; WUGSC:H LUCA16.1
  • 种属：
    Homo sapiens (Human)
  • 蛋白长度：
    Full Length of Mature Protein
  • 表达区域：
    2-355
  • 氨基酸序列
    GCTVSAEDK AAAERSKMID KNLREDGEKA AREVKLLLLG AGESGKSTIV KQMKIIHEDG YSEEECRQYR AVVYSNTIQS IMAIVKAMGN LQIDFADPSR ADDARQLFAL SCTAEEQGVL PDDLSGVIRR LWADHGVQAC FGRSREYQLN DSAAYYLNDL ERIAQSDYIP TQQDVLRTRV KTTGIVETHF TFKDLHFKMF DVGGQRSERK KWIHCFEGVT AIIFCVALSA YDLVLAEDEE MNRMHESMKL FDSICNNKWF TDTSIILFLN KKDLFEEKIT HSPLTICFPE YTGANKYDEA ASYIQSKFED LNKRKDTKEI YTHFTCATDT KNVQFVFDAV TDVIIKNNLK DCGLF
  • 蛋白标签：
    Tag type will be determined during the manufacturing process.
    The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.
  • 产品提供形式：
    Lyophilized powder
    Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
  • 复溶：
    We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
  • 储存条件：
    Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
  • 保质期：
    The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
    Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
  • 货期：
    Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
    Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
  • 注意事项：
    Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
  • Datasheet ：
    Please contact us to get it.

产品评价

靶点详情

  • 功能：
    Guanine nucleotide-binding proteins (G proteins) are involved as modulators or transducers in various transmembrane signaling systems. The G(i) proteins are involved in hormonal regulation of adenylate cyclase: they inhibit the cyclase in response to beta-adrenergic stimuli. May play a role in cell division.; Regulates the cell surface density of dopamine receptors DRD2 by sequestrating them as an intracellular pool.
  • 基因功能参考文献：
    1. The data show that the nonpalmitoylated CB1 receptor significantly reduced its association with Galphai2 . PMID: 28722168
    2. MicroRNA-222-3p/GNAI2/AKT signaling axis inhibits epithelial ovarian cancer cell growth and is associated with good overall survival. PMID: 27811362
    3. Kinocilium is essential for proper localization of Lgn, as well as Gai and aPKC, suggesting that cilium function plays a role in positioning of apical proteins critical for hearing. PMID: 26662512
    4. Data strongly implicate GNAI2 as a critical regulator of oncogenesis and an upstream driver of cancer progression in ovarian carcinoma. PMID: 24423449
    5. Galpha(i2) activates the TRPC4 channel by direct binding. PMID: 25788576
    6. Changes in ion selectivity and pore dilation of the TRPC4 channel elicited by the Galphai2 subunit, were studied. PMID: 24011658
    7. Galphai2(Q205L) regulates satellite cell differentiation into myotubes in a protein kinase C - and histone deacetylase -dependent manner PMID: 24298018
    8. There is an interaction between the activated Gsalpha subunit and membrane lipid microdomains in the pathophysiology of some major depressive disorders. PMID: 23490066
    9. These results suggest that the extent of G-protein-mediated inhibition is significantly reduced in the K1336E mutant CaV2.1 Ca(2+) channels PMID: 23430985
    10. The ubiquitination of Galphai2 and Galphaq is suppressed by expression of Ric-8A. The suppression likely requires Ric-8A interaction with these Galpha proteins; the C-terminal truncation of Galphaq and Galphai2 completely abrogates their interaction with Ric-8A. PMID: 23665327
    11. Gialpha2 plays an essential role in OXT and EGF signaling to induce prostate cancer cell migration. PMID: 22936789
    12. HIV-1 Nef impairs heterotrimeric G-protein signaling by targeting Galpha(i2) for degradation through ubiquitination PMID: 23071112
    13. Galphai2 as a novel claudin-5 partner required for TJ integrity in brain endothelial cells. PMID: 22333621
    14. Suggest that Gnia2 is involved in endothelial TLR pathways along a signaling cascade that is distinct from MyD88. PMID: 21949112
    15. SDF-1 treatment of T cells induced the formation of a novel molecular signaling complex containing RasGRP1, Galphai2, and ZAP-70. PMID: 21856938
    16. Study identified a number of high-confident miR-138 target genes, including proto-oncogene GNAI2, which may play an important role in tongue squamous cell carcinoma. initiation and progression. PMID: 21079996
    17. CXCL12 upregulated T-cell activation, and an alpha(i) G-coupled protein mediated signaling pathway was necessary for stimulation of T cells by CXCL12. PMID: 12088413
    18. Modulation of G(ialpha(2)) signaling by the axonal guidance molecule UNC5H2 PMID: 12359238
    19. valproic acid-induced expression of Galphai2 is regulated by Sp1 transcription factor PMID: 12624107
    20. Cholesterol lowering by pravastatin might increase the response of the heart to parasympathetic stimulation and changes in Galpha(i2) expression might serve as a molecular marker for this effect PMID: 14623802
    21. Galphai2 has a role in calcium-sensing receptor-mediated ERK1/2 activation PMID: 14701866
    22. Abnormal regulation of GNAI2 was studied in cultured skin fibroblasts from insulin-resistant hypertensive individuals. PMID: 15126921
    23. in elderly subjects of similar ages, those with diabetes have 1.7-fold higher levels of Galpha(i2) and twofold higher levels of Gbeta(1). PMID: 15331550
    24. the Galphai2 gene promoter is transcriptionally activated through nuclear factor-kappaB and antioxidant response elements PMID: 15640523
    25. a GNAI2 -318 C>G SNP impairs transcriptional activity through specific binding of Sp1 and is associated with high SBP in Caucasians from Italy PMID: 16565233
    26. These results suggest that Galphai2 is involved specifically in the activation of TRPC4. PMID: 18854172
    27. These results show that G(i2) protein is involved in D(2)R-mediated ERK activation but beta-arrestins 1 and 2 are either not involved or play minor role. PMID: 18940181
    28. This suggests a novel set of candidate genes for NS (GNAI2 and RGS proteins) and if validated could have important implications for therapy as well. PMID: 19282110
    29. Data support the notion that oligomerization of the mu-opioid receptor is not required for agonist and antagonist binding in regard to allosteric regulation of agonist binding by Gi2. PMID: 19542234

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  • 亚细胞定位：
    Cytoplasm. Cytoplasm, cytoskeleton, microtubule organizing center, centrosome. Cell membrane. Membrane; Lipid-anchor. Note=Localizes in the centrosomes of interphase and mitotic cells. Detected at the cleavage furrow and/or the midbody.
  • 蛋白家族：
    G-alpha family, G(i/o/t/z) subfamily
  • 数据库链接：

    HGNC: 4385

    OMIM: 139360

    KEGG: hsa:2771

    STRING: 9606.ENSP00000312999

    UniGene: Hs.77269