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  4. Recombinant Human Growth factor receptor-bound protein 10 (GRB10)

Recombinant Human Growth factor receptor-bound protein 10 (GRB10)

  • 货号：
    CSB-YP009887HU
  • 规格：
  • 来源：
    Yeast
  • 其他：
  • 货号：
    CSB-EP009887HU
  • 规格：
  • 来源：
    E.coli
  • 其他：
  • 货号：
    CSB-EP009887HU-B
  • 规格：
  • 来源：
    E.coli
  • 共轭：
    Avi-tag Biotinylated

    E. coli biotin ligase (BirA) is highly specific in covalently attaching biotin to the 15 amino acid AviTag peptide. This recombinant protein was biotinylated in vivo by AviTag-BirA technology, which method is BriA catalyzes amide linkage between the biotin and the specific lysine of the AviTag.

  • 其他：
  • 货号：
    CSB-BP009887HU
  • 规格：
  • 来源：
    Baculovirus
  • 其他：
  • 货号：
    CSB-MP009887HU
  • 规格：
  • 来源：
    Mammalian cell
  • 其他：

产品详情

  • 纯度：
    >85% (SDS-PAGE)
  • 基因名：
  • Uniprot No.：
  • 别名：
    grb 10; GRB IR; grb-10; GRB10 adapter protein; GRB10 adaptor protein; GRB10; GRB10_HUMAN; GRBIR; Growth factor receptor bound protein 10; Growth factor receptor-bound protein 10; Insulin receptor binding protein; Insulin receptor binding protein GRB IR; Insulin receptor-binding protein Grb-IR; IRBP; KIAA0207; Maternally expressed gene 1; MEG1; RSS
  • 种属：
    Homo sapiens (Human)
  • 蛋白长度：
    full length protein
  • 表达区域：
    1-594
  • 氨基酸序列
    MALAGCPDSF LHHPYYQDKV EQTPRSQQDP AGPGLPAQSD RLANHQEDDV DLEALVNDMN ASLESLYSAC SMQSDTVPLL QNGQHARSQP RASGPPRSIQ PQVSPRQRVQ RSQPVHILAV RRLQEEDQQF RTSSLPAIPN PFPELCGPGS PPVLTPGSLP PSQAAAKQDV KVFSEDGTSK VVEILADMTA RDLCQLLVYK SHCVDDNSWT LVEHHPHLGL ERCLEDHELV VQVESTMASE SKFLFRKNYA KYEFFKNPMN FFPEQMVTWC QQSNGSQTQL LQNFLNSSSC PEIQGFLHVK ELGKKSWKKL YVCLRRSGLY CSTKGTSKEP RHLQLLADLE DSNIFSLIAG RKQYNAPTDH GLCIKPNKVR NETKELRLLC AEDEQTRTCW MTAFRLLKYG MLLYQNYRIP QQRKALLSPF STPVRSVSEN SLVAMDFSGQ TGRVIENPAE AQSAALEEGH AWRKRSTRMN ILGSQSPLHP STLSTVIHRT QHWFHGRISR EESHRIIKQQ GLVDGLFLLR DSQSNPKAFV LTLCHHQKIK NFQILPCEDD GQTFFSLDDG NTKFSDLIQL VDFYQLNKGV LPCKLKHHCI RVAL
  • 蛋白标签：
    Tag type will be determined during the manufacturing process.
    The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.
  • 产品提供形式：
    Lyophilized powder
    Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
  • 复溶：
    We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
  • 储存条件：
    Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
  • 保质期：
    The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
    Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
  • 货期：
    Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
    Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
  • 注意事项：
    Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
  • Datasheet ：
    Please contact us to get it.

产品评价

靶点详情

  • 功能：
    Adapter protein which modulates coupling of a number of cell surface receptor kinases with specific signaling pathways. Binds to, and suppress signals from, activated receptors tyrosine kinases, including the insulin (INSR) and insulin-like growth factor (IGF1R) receptors. The inhibitory effect can be achieved by 2 mechanisms: interference with the signaling pathway and increased receptor degradation. Delays and reduces AKT1 phosphorylation in response to insulin stimulation. Blocks association between INSR and IRS1 and IRS2 and prevents insulin-stimulated IRS1 and IRS2 tyrosine phosphorylation. Recruits NEDD4 to IGF1R, leading to IGF1R ubiquitination, increased internalization and degradation by both the proteasomal and lysosomal pathways. May play a role in mediating insulin-stimulated ubiquitination of INSR, leading to proteasomal degradation. Negatively regulates Wnt signaling by interacting with LRP6 intracellular portion and interfering with the binding of AXIN1 to LRP6. Positive regulator of the KDR/VEGFR-2 signaling pathway. May inhibit NEDD4-mediated degradation of KDR/VEGFR-2.
  • 基因功能参考文献：
    1. Through syngeneic comparison, our study identifies for the first time that Grb10 is associated with the pluripotency state in nuclear transfer embryonic stem cells. PMID: 28476045
    2. Data suggest that GRB10 and GRB14 are both Ca2+-dependent CaM-binding proteins; more than one CaM-binding site and/or accessory CaM-binding sites appear to exist in GRB10 and GRB14, as compared to a single one present in GRB7. (GRB10 = growth factor receptor-bound protein 10; GRB14 = growth factor receptor-bound protein 14; CaM = calmodulin; GRB7 = growth factor receptor-bound protein 7) PMID: 28295264
    3. GRB10 may regulate degradation of the IL-4 receptor-signaling complex through interactions with NEDD4.2. PMID: 27742835
    4. placental expression associated positively with placental weight, birth weight, and neonatal head circumference PMID: 26390806
    5. Risk alleles for 6 loci increased glucose levels from birth to 5 years of age (ADCY5, ADRA2A, CDKAL1, CDKN2A/B, GRB10, and TCF7L2 PMID: 27049325
    6. This study demonstrated that the most significant SNP (rs11770199; p = 0.0003) in single-site analysis was located on chromosome 7 in the GRB10 gene. PMID: 25391383
    7. Association of the intronic polymorphism rs12540874 A>G of the GRB10 gene with high birth weight. PMID: 25103788
    8. Tissue-specific methylation and possibly imprinting of GRB10 can influence glucose metabolism. PMID: 24699409
    9. Studies indicate that insulin receptor (IR) and IGF Type 1 Receptor (IGFR) have been identified as important partners of Grb10/14 and SH2B1/B2 adaptors. PMID: 23190452
    10. H19 and GRB10 methylation was normal in Albright's hereditary osteodystrophy patients. PMID: 22679513
    11. discovery of Grb10 as an mTORC1 substrate PMID: 21659604
    12. Grb10 interacts with Bim L and inhibits its proapoptotic activity in a phosphorylation-dependant manner. PMID: 20174874
    13. GRB10 gene is expressed from the paternal allele in the brain, but from the maternal allele in the placental trophoblasts. PMID: 19487367
    14. role in inhibiting insulin-stimulated insulin receptor substrate (IRS)-phosphatidylinositol 3-kinase/Akt signaling pathway by disrupting the association of IRS-1/IRS-2 with the insulin receptor PMID: 12493740
    15. The structure of this protein's SH domains affect ligand specificity. PMID: 12551896
    16. Grb10 acts as a positive regulator in VEGF-R2 signaling and protects VEGF-R2 from degradation by interacting with Nedd4, a component of the endocytic machinery PMID: 15060076
    17. GRB10 protein products are correlated with parameters of birth size. PMID: 15506681
    18. there is a phosphorylation-regulated complex formation of Grb10 with 14-3-3 and Akt PMID: 15722337
    19. Up-regulation of growth factor receptor-bound protein 10 is associated with cervical squamous cell carcinoma PMID: 15870923
    20. Ser(150) and Ser(476) of human Grb10zeta are phosphorylated in intact cells. PMID: 15952796
    21. Together, our results suggest that, in addition to inhibiting IR kinase activity by directly binding to the IR, Grb10 also negatively regulates insulin signaling by mediating insulin-stimulated degradation of the receptor. PMID: 16434550
    22. Single nucleotide polymorphism is associated with type 2 diabetes among Whites. PMID: 16644667
    23. GRB10 is a novel negative regulator of the Wnt signaling pathway. The finding that GRB10 interferes with the binding of Axin to LRP6 indicated a possible molecular mechanism by which the overexpression of GRB10 suppresses Wnt signaling. PMID: 17376403
    24. Binds to the Raf-1 and MEK1 kinases. Potential role in the regulation of apoptosis. PMID: 9553107
    25. Role in the regulation of mitogenesis by receptor tyrosine kinases. PMID: 10454568
    26. Mostly localized to the mitochondria. Can relocalize to the plasma membrane and lamellipodia under certain conditions. PMID: 10585452

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  • 亚细胞定位：
    Cytoplasm.
  • 蛋白家族：
    GRB7/10/14 family
  • 组织特异性：
    Widely expressed in fetal and adult tissues, including fetal and postnatal liver, lung, kidney, skeletal muscle, heart, spleen, skin and brain.
  • 数据库链接：

    HGNC: 4564

    OMIM: 601523

    KEGG: hsa:2887

    STRING: 9606.ENSP00000381793

    UniGene: Hs.164060