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  4. Recombinant Human Ephexin-1 (NGEF)

Recombinant Human Ephexin-1 (NGEF)

  • 货号：
    CSB-YP850809HU
  • 规格：
  • 来源：
    Yeast
  • 其他：
  • 货号：
    CSB-EP850809HU
  • 规格：
  • 来源：
    E.coli
  • 其他：
  • 货号：
    CSB-EP850809HU-B
  • 规格：
  • 来源：
    E.coli
  • 共轭：
    Avi-tag Biotinylated

    E. coli biotin ligase (BirA) is highly specific in covalently attaching biotin to the 15 amino acid AviTag peptide. This recombinant protein was biotinylated in vivo by AviTag-BirA technology, which method is BriA catalyzes amide linkage between the biotin and the specific lysine of the AviTag.

  • 其他：
  • 货号：
    CSB-BP850809HU
  • 规格：
  • 来源：
    Baculovirus
  • 其他：
  • 货号：
    CSB-MP850809HU
  • 规格：
  • 来源：
    Mammalian cell
  • 其他：

产品详情

  • 纯度：
    >85% (SDS-PAGE)
  • 基因名：
    NGEF
  • Uniprot No.：
  • 别名：
    ARHGEF27; Eph receptor-interacting exchange protein; Eph-interacting exchange protein; EPHEXIN; Ephexin-1; Guanine nucleotide exchange factor, neuronal; Neuronal guanine nucleotide exchange factor; Ngef; NGEF_HUMAN
  • 种属：
    Homo sapiens (Human)
  • 蛋白长度：
    full length protein
  • 表达区域：
    1-710
  • 氨基酸序列
    METRESEDLE KTRRKSASDQ WNTDNEPAKV KPELLPEKEE TSQADQDIQD KEPHCHIPIK RNSIFNRSIR RKSKAKARDN PERNASCLAD SQDNGKSVNE PLTLNIPWSR MPPCRTAMQT DPGAQEMSES SSTPGNGATP EEWPALADSP TTLTEALRMI HPIPADSWRN LIEQIGLLYQ EYRDKSTLQE IETRRQQDAE IEDNTNGSPA SEDTPEEEEE EEEEEEPASP PERKTLPQIC LLSNPHSRFN LWQDLPEIRS SGVLEILQPE EIKLQEAMFE LVTSEASYYK SLNLLVSHFM ENERIRKILH PSEAHILFSN VLDVLAVSER FLLELEHRME ENIVISDVCD IVYRYAADHF SVYITYVSNQ TYQERTYKQL LQEKAAFREL IAQLELDPKC RGLPFSSFLI LPFQRITRLK LLVQNILKRV EERSERECTA LDAHKELEMV VKACNEGVRK MSRTEQMISI QKKMEFKIKS VPIISHSRWL LKQGELQQMS GPKTSRTLRT KKLFHEIYLF LFNDLLVICR QIPGDKYQVF DSAPRGLLRV EELEDQGQTL ANVFILRLLE NADDREATYM LKASSQSEMK RWMTSLAPNR RTKFVSFTSR LLDCPQVQCV HPYVAQQPDE LTLELADILN ILDKTDDGWI FGERLHDQER GWFPSSMTEE ILNPKIRSQN LKECFRVHKM DDPQRSQNKD RRKLGSRNRQ
  • 蛋白标签：
    Tag type will be determined during the manufacturing process.
    The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.
  • 产品提供形式：
    Lyophilized powder
    Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
  • 复溶：
    We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
  • 储存条件：
    Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
  • 保质期：
    The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
    Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
  • 货期：
    Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
    Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
  • 注意事项：
    Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
  • Datasheet ：
    Please contact us to get it.

产品评价

靶点详情

  • 功能：
    Acts as a guanine nucleotide exchange factor (GEF) which differentially activates the GTPases RHOA, RAC1 and CDC42. Plays a role in axon guidance regulating ephrin-induced growth cone collapse and dendritic spine morphogenesis. Upon activation by ephrin through EPHA4, the GEF activity switches toward RHOA resulting in its activation. Activated RHOA promotes cone retraction at the expense of RAC1- and CDC42-stimulated growth cone extension.
  • 基因功能参考文献：
    1. A visceral-fat-associated SNP, rs11678490 of NGEF, was found in Korean men. PMID: 26340433
    2. FGFR-mediated phosphorylation of ephexin1 enhances the guanine nucleotide exchange activity toward RhoA without affecting the activity to Rac1 or Cdc42. PMID: 17702745
  • 亚细胞定位：
    Cytoplasm. Membrane. Cell projection, growth cone.
  • 组织特异性：
    Highly expressed in brain specifically in caudate nucleus and to a lower extent in amygdala and hippocampus. Also detected in lung.
  • 数据库链接：

    HGNC: 7807

    OMIM: 605991

    KEGG: hsa:25791

    STRING: 9606.ENSP00000264051

    UniGene: Hs.97316