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  4. Recombinant Human ETS translocation variant 4 (ETV4)

Recombinant Human ETS translocation variant 4 (ETV4)

  • 货号：
    CSB-YP007858HU
  • 规格：
  • 来源：
    Yeast
  • 其他：
  • 货号：
    CSB-EP007858HU
  • 规格：
  • 来源：
    E.coli
  • 其他：
  • 货号：
    CSB-EP007858HU-B
  • 规格：
  • 来源：
    E.coli
  • 共轭：
    Avi-tag Biotinylated

    E. coli biotin ligase (BirA) is highly specific in covalently attaching biotin to the 15 amino acid AviTag peptide. This recombinant protein was biotinylated in vivo by AviTag-BirA technology, which method is BriA catalyzes amide linkage between the biotin and the specific lysine of the AviTag.

  • 其他：
  • 货号：
    CSB-BP007858HU
  • 规格：
  • 来源：
    Baculovirus
  • 其他：
  • 货号：
    CSB-MP007858HU
  • 规格：
  • 来源：
    Mammalian cell
  • 其他：

产品详情

  • 纯度：
    >85% (SDS-PAGE)
  • 基因名：
    ETV4
  • Uniprot No.：
  • 别名：
    Adenovirus E1A enhancer binding protein; Adenovirus E1A enhancer-binding protein; E1A F; E1A-F; ETS translocation variant 4; ets variant gene 4 (E1A enhancer binding protein E1AF); ETV4; ETV4_HUMAN; PEA3; PEA3 protein; PEAS3; Polyomavirus enhancer activator 3; Polyomavirus enhancer activator 3 homolog; Protein PEA3
  • 种属：
    Homo sapiens (Human)
  • 蛋白长度：
    Full length protein
  • 表达区域：
    1-484
  • 氨基酸序列
    MERRMKAGYL DQQVPYTFSS KSPGNGSLRE ALIGPLGKLM DPGSLPPLDS EDLFQDLSHF QETWLAEAQV PDSDEQFVPD FHSENLAFHS PTTRIKKEPQ SPRTDPALSC SRKPPLPYHH GEQCLYSSAY DPPRQIAIKS PAPGALGQSP LQPFPRAEQR NFLRSSGTSQ PHPGHGYLGE HSSVFQQPLD ICHSFTSQGG GREPLPAPYQ HQLSEPCPPY PQQSFKQEYH DPLYEQAGQP AVDQGGVNGH RYPGAGVVIK QEQTDFAYDS DVTGCASMYL HTEGFSGPSP GDGAMGYGYE KPLRPFPDDV CVVPEKFEGD IKQEGVGAFR EGPPYQRRGA LQLWQFLVAL LDDPTNAHFI AWTGRGMEFK LIEPEEVARL WGIQKNRPAM NYDKLSRSLR YYYEKGIMQK VAGERYVYKF VCEPEALFSL AFPDNQRPAL KAEFDRPVSE EDTVPLSHLD ESPAYLPELA GPAQPFGPKG GYSY
  • 蛋白标签：
    Tag type will be determined during the manufacturing process.
    The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.
  • 产品提供形式：
    Lyophilized powder
    Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
  • 复溶：
    We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
  • 储存条件：
    Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
  • 保质期：
    The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
    Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
  • 货期：
    Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
    Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
  • 注意事项：
    Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
  • Datasheet ：
    Please contact us to get it.

产品评价

靶点详情

  • 功能：
    Transcriptional activator. May play a role in keratinocyte differentiation.; (Microbial infection) Binds to the enhancer of the adenovirus E1A gene and acts as a transcriptional activator; the core-binding sequence is 5'-[AC]GGA[AT]GT-3'.
  • 基因功能参考文献：
    1. Experimental analysis showed the importance of LEF1, ETV4 and FABP6 as three co-regulated prognostic markers in patients with colorectal cancer metastasis. PMID: 30193961
    2. Many of the ewing sarcoma family of tumors with negative NKX2.2 immunoreactivity were in bone. Strong/moderate ETV4 nuclear expression was detected in two small round cell tumors PMID: 28864350
    3. FOS strongly binds to the same MED25 site as ETV4 activation domain and JUN interacts with the other two MED25 sites. PMID: 28728983
    4. ETV4 overexpression is associated with lung cancer metastasis. PMID: 27869830
    5. The data provide the molecular details of ETV4-mediated NANOG gene expression. PMID: 28412366
    6. NCOA2ETV4 protein would contain the helixloophelix, PAS_9 and PAS_11, CITED domains, the SRC1 domain of NCOA2 and the ETS DNAbinding domain of ETV4. PMID: 27633981
    7. PEA3-subgroup transcription factors are key players of the Met signaling integration involved in regulation of migration and invasiveness of tumor cells. PMID: 26238631
    8. ACC1 and ACLY regulate the levels of ETV4 under hypoxia via increased alpha-ketoglutarate. These results reveal that the ACC1/ACLY-alpha-ketoglutarate-ETV4 axis is a novel means by which metabolic states regulate transcriptional output PMID: 26452058
    9. ETV4 overexpression is associated with prostate cancer aggressiveness. PMID: 25595908
    10. molecular mechanism underlying DBC1 function in PEA3-mediated transcription involves inhibition of SIRT1 interaction with PEA3 and of SIRT1-mediated deacetylation of PEA3 PMID: 25417701
    11. Implicate Pea3 as a mediator of liver metastases in colorectal cancer. PMID: 25516649
    12. Silencing of ETV4 suppresses invasion of prostate cancer cells by inhibiting the expression of uPA/uPAR as well as (MMP)-2 and MMP-9 PMID: 25544710
    13. data suggest that polyomavirus enhancer activator 3 and epithelium-specific transcription factor-1 may play important roles in pluri potent and tumorigenic embryonic carcinoma cells. PMID: 24694612
    14. PEA3 regulates OCT4 gene expression as a transcriptional activator PMID: 24983502
    15. Study reveals molecular insight into how the Ets family transcription factor Pea3 favors EMT and contributes to tumorigenesis via a negative regulatory loop with Cyclin D2, a new Pea3 target gene. PMID: 23989931
    16. Two members of the ETS (E-26) family (PEA3 and ELK-1) regulate the expression of miRNA-200b. PEA3 promotes the expression of miRNA-200b, and ELK-1 is a transcriptional repressor of miRNA-200b. PMID: 24072701
    17. Review evidence for a role of ETV1, 4 and 5 as oncoproteins and describe modes of their action. PMID: 22425584
    18. Notch-1 and Notch-4 are novel transcriptional targets of PEA3 in breast cancer cells. PMID: 21679465
    19. These results indicated that PEA3 could activate CXCR4 promoter transcription and promote breast cancer metastasis. PMID: 21831961
    20. Heterogeneous nuclear ribonucleoprotein-like protein CoAA interacts with PEA3 group member proteins and modulates their transcriptional activity. PMID: 21736557
    21. High PEA3 expression is associated with gastric adenocarcinoma. PMID: 21673681
    22. Study shows that the ERK-PEA3-MMP-1 axis is upregulated in oesophageal adenocarcinoma cells and is a potentially important driver of the metastatic progression of oesophageal adenocarcinomas. PMID: 21143918
    23. Knockdown of MyoD and PEA3 attenuated MDR1 expression and increased the sensitivity of multidrug resistant cancer cells to cytotoxic drugs that were transported by P-gp in SGC7901/VCR cells. PMID: 20980337
    24. human capicua represses mRNA expression for PEA3 (polyoma enhancer activator 3) Ets transcription factors ETV1, ETV4 and ETV5 PMID: 21087211
    25. PEA3 has a central role in tumor progression in ovarian carcinoma PMID: 12684413
    26. No association of E1AF levels with HER2/neu mRNA levels, hormone receptor status, histological grade, tumor size, lymph node involvement or prognosis was found. PMID: 12779089
    27. role in ovarian and breast malignancies and suggests that it may be target for therapeutic intervention PMID: 15387369
    28. PEA3, by its capacity to up-regulate the epithelial marker MUC4 and to down-regulate the ErbB-2 oncogene, appears as a key regulator of the differentiation/proliferation balance in pancreatic cancer cells. PMID: 15461591
    29. E1AF has an essential role in the activation of the human GalT I gene in highly metastatic lung cancer cells PMID: 15611127
    30. E1AF was modified by ubiquitin through the C-terminal region and ubiquitinated E1AF aggregated in nuclear dots, and the inhibition of proteasome-activated transcription from E1AF target promoters PMID: 15629152
    31. important role in the early stage of colorectal carcinogenesis PMID: 15695237
    32. E1AF positively regulates transcription from MT1-MMP genes, which plays an important role in invasion and metastasis of squamous cell carcinoma of the tongue by converting pro-MMP-2 into active-MMP-2 PMID: 15756447
    33. results suggest E1A-F is overexpressed in early stages of human CRC development and may be an important factor in the overexpression of COX-2 and MMP-7 PMID: 15800927
    34. The ETV4 protein was localised to nuclei of spermatogonia and revealed an intense staining in seminoma cells. PMID: 16158187
    35. E1AF is a housekeeping gene, whose expression is controlled in specific tissues. PMID: 16297865
    36. Overexpression of ETV4 is identified in 2 of 98 cases of prostate cancer. PMID: 16585160
    37. These results provide evidence for the antitumor activity of PEA3 in human breast cancers. PMID: 16652376
    38. These data suggest that ETV4 and Myeov may provide novel targets for therapeutic intervention. PMID: 16678123
    39. By manipulating LPP levels, we show that it acts to upregulate the transactivation capacity of PEA3. PMID: 16738319
    40. PEA3 expression is not correlated with HER-2/neu expression in breast cancer tumor tissues PMID: 16752078
    41. PEA3 and c-Jun stimulate synergistically the HER2/neu gene transcription with p300 PMID: 16786139
    42. PEA3 stabilization due to LKB1 inactivation could lead to epithelial/mesenchymal transition and greater lung cancer invasion potential. PMID: 16912160
    43. Overexpression of PEA3 is associated with breast tumour progression PMID: 17060941
    44. E1AF increases cell cycle progression via upregulation of Cyclin D3 transcription, which elicits a new mechanism of breast cancer growth and a new mechanism of Cyclin D3 transcription. PMID: 17467662
    45. These results suggest that PEA3 is regulated by EGFR and that the elevated PEA3 expression detected in human ovarian cancer may divert cells to a more invasive phenotype by regulating MMP-9 and MMP-14. PMID: 17475671
    46. Our results indicate that PIASy negatively regulates E1AF-mediated transcription by both E1AF sumoylation in a dependent and independent fashion. PMID: 17585876
    47. This report describes a new mechanism of glioma invasion involving a cooperative effort between E1AF and Sp1 transcription factors. PMID: 17938207
    48. E1AF plays a crucial role in the invasion and metastasis of malignant melanoma through up-regulating the MT1-MMP expression PMID: 18425363
    49. The two novel ETV4 fusion partners possess as predominant common characteristics androgen-induction and prostate-specific expression. PMID: 18451133
    50. E1AF overexpression markedly enhanced mithramycin A-induced Huh-7 cell apoptosis and the expression of pro-apoptotic protein Bax depending on its DNA-binding domain. PMID: 18510939

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  • 亚细胞定位：
    Nucleus.
  • 蛋白家族：
    ETS family
  • 组织特异性：
    Expressed in keratinocytes.
  • 数据库链接：

    HGNC: 3493

    OMIM: 600711

    KEGG: hsa:2118

    STRING: 9606.ENSP00000321835

    UniGene: Hs.434059