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  4. Recombinant Human DNA-directed RNA polymerase I subunit RPA34 (CD3EAP)

Recombinant Human DNA-directed RNA polymerase I subunit RPA34 (CD3EAP)

  • 货号：
    CSB-YP004932HU
  • 规格：
  • 来源：
    Yeast
  • 其他：
  • 货号：
    CSB-EP004932HU
  • 规格：
  • 来源：
    E.coli
  • 其他：
  • 货号：
    CSB-EP004932HU-B
  • 规格：
  • 来源：
    E.coli
  • 共轭：
    Avi-tag Biotinylated

    E. coli biotin ligase (BirA) is highly specific in covalently attaching biotin to the 15 amino acid AviTag peptide. This recombinant protein was biotinylated in vivo by AviTag-BirA technology, which method is BriA catalyzes amide linkage between the biotin and the specific lysine of the AviTag.

  • 其他：
  • 货号：
    CSB-BP004932HU
  • 规格：
  • 来源：
    Baculovirus
  • 其他：
  • 货号：
    CSB-MP004932HU
  • 规格：
  • 来源：
    Mammalian cell
  • 其他：

产品详情

  • 纯度：
    >85% (SDS-PAGE)
  • 基因名：
    CD3EAP
  • Uniprot No.：
  • 别名：
    A34.5; Anti sense to ERCC 1 protein; Antisense to ERCC-1 protein; ASE 1; ASE-1; ASE1; CAST; CD3 epsilon associated protein; CD3-epsilon-associated protein; CD3E antigen, epsilon polypeptide associated protein; CD3E associated protein; CD3e molecule, epsilon associated protein; CD3E-associated protein; CD3EAP; DNA directed RNA polymerase I subunit RPA34; DNA-directed RNA polymerase I subunit RPA34; MGC118851; PAF 49; PAF49; RNA polymerase I associated factor PAF49; RNA polymerase I-associated factor PAF49; RPA34_HUMAN
  • 种属：
    Homo sapiens (Human)
  • 蛋白长度：
    Full length protein
  • 表达区域：
    1-510
  • 氨基酸序列
    MEEPQAGDAA RFSCPPNFTA KPPASESPRF SLEALTGPDT ELWLIQAPAD FAPECFNGRH VPLSGSQIVK GKLAGKRHRY RVLSSCPQAG EATLLAPSTE AGGGLTCASA PQGTLRILEG PQQSLSGSPL QPIPASPPPQ IPPGLRPRFC AFGGNPPVTG PRSALAPNLL TSGKKKKEMQ VTEAPVTQEA VNGHGALEVD MALGSPEMDV RKKKKKKNQQ LKEPEAAGPV GTEPTVETLE PLGVLFPSTT KKRKKPKGKE TFEPEDKTVK QEQINTEPLE DTVLSPTKKR KRQKGTEGME PEEGVTVESQ PQVKVEPLEE AIPLPPTKKR KKEKGQMAMM EPGTEAMEPV EPEMKPLESP GGTMAPQQPE GAKPQAQAAL AAPKKKTKKE KQQDATVEPE TEVVGPELPD DLEPQAAPTS TKKKKKKKER GHTVTEPIQP LEPELPGEGQ PEARATPGST KKRKKQSQES RMPETVPQEE MPGPPLNSES GEEAPTGRDK KRKQQQQQPV
  • 蛋白标签：
    Tag type will be determined during the manufacturing process.
    The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.
  • 产品提供形式：
    Lyophilized powder
    Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
  • 复溶：
    We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
  • 储存条件：
    Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
  • 保质期：
    The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
    Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
  • 货期：
    Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
    Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
  • 注意事项：
    Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
  • Datasheet ：
    Please contact us to get it.

产品评价

靶点详情

  • 功能：
    DNA-dependent RNA polymerase catalyzes the transcription of DNA into RNA using the four ribonucleoside triphosphates as substrates. Component of RNA polymerase I which synthesizes ribosomal RNA precursors. Isoform 1 is involved in UBTF-activated transcription, presumably at a step following PIC formation.; Isoform 2 has been described as a component of preformed T-cell receptor (TCR) complex.
  • 基因功能参考文献：
    1. Haplotypes consisting of PPP1R13L, CD3EAP and GLTSCR1 SNPs on Chr19q13.3 may associate with lung cancer risk in the Chinese population. PMID: 30128886
    2. a genetic variant in the CD3EAP gene is associated with outcome of patients treated for multiple myeloma PMID: 26568154
    3. These findings demonstrate that 19p13.3-GADD45B rs7354 variant and interaction between 19p13.3-GADD45B rs3783501 and 19q13.3-CD3EAP rs967591 may play a role in association with smoke-exposed lung cancer among Chinese. PMID: 28870783
    4. There was no interaction between NFKB1 polymorphisms and PPP1R13L and CD3EAP and smoking status in relation to lung cancer risk PMID: 25917613
    5. In nonsmoking Chinese women CD3EAP rs967591 variant allele carriers are at increased risk of lung cancer. PMID: 23624123
    6. Polymorphism in RAI and CD3EAP are associated with outcome of myeloma patients treated with high dose treatment. PMID: 21046104
    7. model for CAST/hPAF49 function in which the network of interactions of Pol I-specific subunits with UBF facilitates conformational changes of the polymerase, leading to stabilization of the Pol I-template complex and, thereby, activation of transcription PMID: 16809778
    8. The ASE-1 polymorphism was associated with an increased risk of adenomas, OR of 1.39 (95% CI 1.06-1.81), which upon stratification was apparent among women only, OR of 1.66 (95% CI 1.15-2.39) PMID: 16817948
    9. ASE-1 polymorphisms and the previously identified ASE-1 haplotype are not associated with risk of colorectal cancer. PMID: 18289367
    10. A SNP in the 5' noncoding region of exon 1 at position -21 from the first codon, which does not affect the final AA sequence but may affect transcription/translation, was associated with drug sensitivity in the NC160 human cancer cell line panel. PMID: 19396015
    11. ASE-1 co-localises with the RNA polymerase I transcription initiation factor UBF/NOR-90 throughout all stages of the cell cycle and these two proteins associate with each other in vitro. PMID: 9426281

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  • 亚细胞定位：
    Nucleus, nucleolus. Chromosome. Note=Found at the fibrillar centers of the nucleolus in interphase and during cell division it is localized to the nucleolus organizer regions of the chromosomes.
  • 蛋白家族：
    Eukaryotic RPA34 RNA polymerase subunit family
  • 数据库链接：

    HGNC: 24219

    OMIM: 107325

    KEGG: hsa:10849

    STRING: 9606.ENSP00000310966

    UniGene: Hs.710495