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  4. Recombinant Human Cullin-5 (CUL5)

Recombinant Human Cullin-5 (CUL5)

  • 货号：
    CSB-YP835890HU
  • 规格：
  • 来源：
    Yeast
  • 其他：
  • 货号：
    CSB-EP835890HU-B
  • 规格：
  • 来源：
    E.coli
  • 共轭：
    Avi-tag Biotinylated

    E. coli biotin ligase (BirA) is highly specific in covalently attaching biotin to the 15 amino acid AviTag peptide. This recombinant protein was biotinylated in vivo by AviTag-BirA technology, which method is BriA catalyzes amide linkage between the biotin and the specific lysine of the AviTag.

  • 其他：
  • 货号：
    CSB-BP835890HU
  • 规格：
  • 来源：
    Baculovirus
  • 其他：
  • 货号：
    CSB-MP835890HU
  • 规格：
  • 来源：
    Mammalian cell
  • 其他：

产品详情

  • 纯度：
    >85% (SDS-PAGE)
  • 基因名：
  • Uniprot No.：
  • 别名：
    CUL 5; CUL-5; CUL5; CUL5_HUMAN; Cullin 5; Cullin-5 (vasopressin-activated calcium-mobilizing receptor-1); Cullin-5; Cullin5; VACM 1; VACM-1; VACM1; Vasopressin activated calcium mobilizing receptor 1; Vasopressin activated calcium mobilizing receptor; Vasopressin-activated calcium-mobilizing receptor 1; Vasopressin-activated calcium-mobilizing receptor-1
  • 种属：
    Homo sapiens (Human)
  • 蛋白长度：
    Full Length
  • 表达区域：
    1-780
  • 氨基酸序列
    ATSNLLKNK GSLQFEDKWD FMRPIVLKLL RQESVTKQQW FDLFSDVHAV CLWDDKGPAK IHQALKEDIL EFIKQAQARV LSHQDDTALL KAYIVEWRKF FTQCDILPKP FCQLEITLMG KQGSNKKSNV EDSIVRKLML DTWNESIFSN IKNRLQDSAM KLVHAERLGE AFDSQLVIGV RESYVNLCSN PEDKLQIYRD NFEKAYLDST ERFYRTQAPS YLQQNGVQNY MKYADAKLKE EEKRALRYLE TRRECNSVEA LMECCVNALV TSFKETILAE CQGMIKRNET EKLHLMFSLM DKVPNGIEPM LKDLEEHIIS AGLADMVAAA ETITTDSEKY VEQLLTLFNR FSKLVKEAFQ DDPRFLTARD KAYKAVVNDA TIFKLELPLK QKGVGLKTQP ESKCPELLAN YCDMLLRKTP LSKKLTSEEI EAKLKEVLLV LKYVQNKDVF MRYHKAHLTR RLILDISADS EIEENMVEWL REVGMPADYV NKLARMFQDI KVSEDLNQAF KEMHKNNKLA LPADSVNIKI LNAGAWSRSS EKVFVSLPTE LEDLIPEVEE FYKKNHSGRK LHWHHLMSNG IITFKNEVGQ YDLEVTTFQL AVLFAWNQRP REKISFENLK LATELPDAEL RRTLWSLVAF PKLKRQVLLY EPQVNSPKDF TEGTLFSVNQ EFSLIKNAKV QKRGKINLIG RLQLTTERMR EEENEGIVQL RILRTQEAII QIMKMRKKIS NAQLQTELVE ILKNMFLPQK KMIKEQIEWL IEHKYIRRDE SDINTFIYMA
  • 蛋白标签：
    Tag type will be determined during the manufacturing process.
    The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.
  • 产品提供形式：
    Lyophilized powder
    Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
  • 复溶：
    We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
  • 储存条件：
    Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
  • 保质期：
    The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
    Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
  • 货期：
    Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
    Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
  • 注意事项：
    Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
  • Datasheet ：
    Please contact us to get it.

产品评价

靶点详情

  • 功能：
    Core component of multiple SCF-like ECS (Elongin-Cullin 2/5-SOCS-box protein) E3 ubiquitin-protein ligase complexes, which mediate the ubiquitination and subsequent proteasomal degradation of target proteins. As a scaffold protein may contribute to catalysis through positioning of the substrate and the ubiquitin-conjugating enzyme. The functional specificity of the E3 ubiquitin-protein ligase complex depends on the variable substrate recognition component. ECS(SOCS1) seems to direct ubiquitination of JAK2. ECS(KLHDC1) complex is part of the DesCEND (destruction via C-end degrons) pathway and mediates ubiquitination and degradation of truncated SELENOS selenoprotein produced by failed UGA/Sec decoding, which ends with a glycine. May form a cell surface vasopressin receptor.; (Microbial infection) Seems to be involved in proteosomal degradation of p53/TP53 stimulated by adenovirus E1B-55 kDa protein.
  • 基因功能参考文献：
    1. CUL5 may be an important part of the mechanism for thalidomide-dependent inhibition. PMID: 29746508
    2. These findings report genetic variants possibly associated with susceptibility to HIV-1 infection (CUL5 rs11212495, rs7103534, rs7117111) and partial viral load control (APOBEC3G rs2294367). PMID: 28302043
    3. structural model suggests that the diverse FIV and HIV-1 Vifs use conserved residues for Cullin 5 binding, FIV Vif binds Cullin 5 independently of zinc, in contrast to HIV-1 Vif. PMID: 29263270
    4. these findings identify Cul-5 as a signaling component that connects an LPS-activated TLR4-MyD88 complex to TRAF6 for efficient activation of NF-kappaB PMID: 27233966
    5. CUL5 neddylation may allosterically tune polyubiquitin chain length and topology. PMID: 28082425
    6. Study found that CUL5 expression was significantly decreased in both endometrioid adenocarcinomas with the more aggressive serous type displaying a higher reduction. Its mRNA and protein overexpression in endometrial cancer cells resulted in decreased cell proliferation. PMID: 26847831
    7. Studies indicate that Cullin-RING E3 ubiquitin ligases (CRL) are key players of the ubiquitylation pathway. PMID: 26253693
    8. These results provide important information on the assembly of the Vif-CUL5-E3 ubiquitin ligase and identify a new viV binding interface with CBF-beta at the C-terminus of HIV-1 Vif. PMID: 25424878
    9. Data indicate that cell surface adhesion molecules VCAM-1 and P-selectin play some roles in mechanical stretch-induced HL-60 cell adhesion to mouse common carotid arteries. PMID: 25108430
    10. N-terminal mutants of HIV-1 vif that demonstrated reduced Cul5 binding were also unable to degrade APOBEC3G as well as APOBEC3F. PMID: 24422669
    11. A tumor suppressor gene, CUL5, is identified as a direct target of miR-7 in hepatocellular carcinoma. PMID: 24339204
    12. Silencing CUL5 reduced cellular sensitivity to three distinct HSP90 inhibitors, across four cancer types driven by different protein kinases. PMID: 24760825
    13. In the absence of CBFbeta, Vif does not bind Cul5, thus preventing the assembly of the E3 ligase complex. PMID: 24390320
    14. CBF-beta is critical for the formation of the Vif-ElonginB/ElonginC-Cul5 core E3 ubiquitin ligase complex. PMID: 24390335
    15. data reveal the structural basis for Vif hijacking of the CBF-beta and CUL5 E3 ligase complex, laying a foundation for rational design of novel anti-HIV drugs PMID: 24402281
    16. ASB9 is unstable alone but forms a stable ternary complex with EloBC that binds with high affinity to the Cullin 5 N-terminal domain. PMID: 23837592
    17. crystal structure shows interaction between SOCS2-elongin BC and Cullin-5 PMID: 23897481
    18. Data indicate tht n the Biaka, strong signal of selection was detected at CUL5 and at TSG101. PMID: 23217182
    19. comparison of heat capacity changes supports a model in which CBFbeta prestabilizes Vif((1-192)) relative to Vif((95-192)), consistent with a stronger interaction of Cul5 with Vif's C-terminal Zn(2+)-binding motif. PMID: 23098073
    20. detected a reduced editing associated with the CUL5 SNP6 minor allele and also with certain Vif variants (mutations at sites 46, 122, and 160). PMID: 22145963
    21. Vif alterations may contribute to a rapid AIDS onset and Vif variability could be influenced by APOBEC3G and CUL5 polymorphisms in children PMID: 21571098
    22. study showed VACM-1 DNA in T47D cancer cells is not mutated; SNP found in U138MG, OVCAR-3 and ACHN cell lines results in silent mutation; results suggest in T47D cancer cells, VACM-1 activity may controlled by epigenetic or posttranslational modification PMID: 21635549
    23. Cul5 interacts with the Hsp90 chaperone complex and is recruited to the site of ErbB2 on the plasma membrane, thereby inducing its polyubiquitination and proteasome-mediated degradation. PMID: 19933325
    24. findings report that HIV-1 Vif interacts with cellular proteins Cul5, elongins B and C, and Rbx1 to form an Skp1-cullin-F-box (SCF)-like complex PMID: 14564014
    25. These data indicate that vasopressin-activated calcium-mobilizing (VACM-1) is involved in the regulation of cellular growth. PMID: 15184056
    26. Cul5 promotes vif ubiquination and requires intact SOCS-box PMID: 15574592
    27. the E3 ubiquitin ligase activity of the Vif-BC-Cul5 complex is essential for Vif function against APOBEC3G PMID: 15781449
    28. findings indicate that the assembly of Vif-Cul5 E3 ubiquitin ligases requires a hydrophobic interface within a novel zinc-binding domain in the substrate receptor Vif and a unique region in Cul5 PMID: 16530799
    29. the zinc-binding region in Vif is a novel cullin interaction domain that mediates selective binding to Cul5 PMID: 16636053
    30. Data indicate that in the T47D cancer cell line VACM-1 inhibits growth by attenuating estrogen-dependent/estrogen receptor alpha signaling responses. PMID: 17186378
    31. Adenovirus E4orf6-mediated p53 degradation requires Cul5. PMID: 17351129
    32. CUL5, together with NPAT and PPP2R1B, is implicated in the deregulation of the cell-cycle and apoptosis regulators and in the pathogenesis of B-CLL. PMID: 17449237
    33. Cul5 plays an essential role in regulating neuron migrations during cortical development, possibly by opposing a promigratory effect of Dab1. PMID: 17974915
    34. Degradation of AAV5 proteins can be inhibited by a dominant-negative ubiquitin that prevents chain elongation or by small interfering RNA directed against cullin 5. PMID: 18216112
    35. The purpose of this study was to determine if there is increased expression of Cul5 during granulocytic differentiation of HL-60 cells. PMID: 19118439
    36. HIV-1 virion infectivity factor (Vif) protein-cullin 5 (Cul5) interaction is mediated by zinc binding to the conserved Vif HCCH motif. Zinc enhances the Vif HCCH-Cul5 interaction by 8-fold. PMID: 19588889

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  • 蛋白家族：
    Cullin family
  • 数据库链接：

    HGNC: 2556

    OMIM: 601741

    KEGG: hsa:8065

    STRING: 9606.ENSP00000376808

    UniGene: Hs.440320