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  4. Recombinant Human Crk-like protein (CRKL)

Recombinant Human Crk-like protein (CRKL)

  • 货号：
    CSB-YP005980HU
  • 规格：
  • 来源：
    Yeast
  • 其他：
  • 货号：
    CSB-EP005980HU
  • 规格：
  • 来源：
    E.coli
  • 其他：
  • 货号：
    CSB-EP005980HU-B
  • 规格：
  • 来源：
    E.coli
  • 共轭：
    Avi-tag Biotinylated

    E. coli biotin ligase (BirA) is highly specific in covalently attaching biotin to the 15 amino acid AviTag peptide. This recombinant protein was biotinylated in vivo by AviTag-BirA technology, which method is BriA catalyzes amide linkage between the biotin and the specific lysine of the AviTag.

  • 其他：
  • 货号：
    CSB-BP005980HU
  • 规格：
  • 来源：
    Baculovirus
  • 其他：
  • 货号：
    CSB-MP005980HU
  • 规格：
  • 来源：
    Mammalian cell
  • 其他：

产品详情

  • 纯度：
    >85% (SDS-PAGE)
  • 基因名：
  • Uniprot No.：
  • 别名：
    Crk L; Crk like protein; Crk-like protein; Crkl; CRKL_HUMAN; Crkol; HGNC:2363 ; Oncogene CrkL; V crk avian sarcoma virus CT10 oncogene homolog like; v crk sarcoma virus CT10 oncogene homolog (avian) like; V crk sarcoma virus CT10 oncogene homolog avian like
  • 种属：
    Homo sapiens (Human)
  • 蛋白长度：
    Full length protein
  • 表达区域：
    1-303
  • 氨基酸序列
    MSSARFDSSD RSAWYMGPVS RQEAQTRLQG QRHGMFLVRD SSTCPGDYVL SVSENSRVSH YIINSLPNRR FKIGDQEFDH LPALLEFYKI HYLDTTTLIE PAPRYPSPPM GSVSAPNLPT AEDNLEYVRT LYDFPGNDAE DLPFKKGEIL VIIEKPEEQW WSARNKDGRV GMIPVPYVEK LVRSSPHGKH GNRNSNSYGI PEPAHAYAQP QTTTPLPAVS GSPGAAITPL PSTQNGPVFA KAIQKRVPCA YDKTALALEV GDIVKVTRMN INGQWEGEVN GRKGLFPFTH VKIFDPQNPD ENE
  • 蛋白标签：
    Tag type will be determined during the manufacturing process.
    The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.
  • 产品提供形式：
    Lyophilized powder
    Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
  • 复溶：
    We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
  • 储存条件：
    Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
  • 保质期：
    The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
    Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
  • 货期：
    Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
    Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
  • 注意事项：
    Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
  • Datasheet ：
    Please contact us to get it.

产品评价

靶点详情

  • 功能：
    May mediate the transduction of intracellular signals.
  • 基因功能参考文献：
    1. Taken together, our data demonstrated that miR-429 might function as an antimetastatic miRNA to regulate HCC metastasis by directly targeting CRKL via modulating Raf/MEK/ERK-epithelial mesenchymal transition pathway. PMID: 29403024
    2. High CRKL expression is associated with Cervical Cancer. PMID: 29295725
    3. Silencing CRKL expression in PTEN-null human cancer cells leads to a decrease in p110beta-dependent PI3K signaling and cell proliferation. PMID: 28723560
    4. Study found the expression of CRKL up-regulated and correlated with that of ABCG2 in gastric neoplasm. CRKL is one of the downstream molecules regulated by ABCG2, which promotes cell growth. PMID: 28029654
    5. We demonstrated that CRKL is a novel downstream effector of ALK signaling in non-small-cell lung cancer PMID: 27078848
    6. Dynamic multi-site phosphorylation by Fyn and Abl drives the interaction between CRKL and the novel scaffolding receptors DCBLD1 and DCBLD2. PMID: 29025973
    7. Data show that miR-193b, by directly targeting focal adhesion kinase (FAK), CRK-like proto-oncogene (CRKL), and methionine sulfoxide reductase A (MSRA), regulates focal adhesion signaling and ROS signaling, which play pivotal roles in liposarcomagenesis and adipogenic differentiation. PMID: 28882999
    8. TP53-miR-215-PCAT-1-CRKL axis might represent an important regulatory pathway in hepatocellular carcinoma. PMID: 28887306
    9. The impaired T-cell proliferation and reduction of CRKL, phosphorylated CRKL, and c-Fos levels suggest a possible role of CRKL in functional deficiencies of T cells in patients with pDGS. PMID: 26875746
    10. he findings in this study indicate a regulation relationship between CRKL and SLC7A5, and provide useful evidence for gastric cancer therapeutic strategies. PMID: 27846244
    11. We identified a recurrent 370-kb deletion at the 22q11.2 locus as a driver of kidney defects in the DiGeorge syndrome and in sporadic congenital kidney and urinary tract anomalies. SNAP29, AIFM3, and CRKL appear to be critical to the phenotype, with haploinsufficiency of CRKL emerging as the main genetic driver. PMID: 28121514
    12. a role for miR-429 as a novel target suppressing invasion and migration of human cervical cancer cells through modulation of its targeting genes ZEB1 and CRKL, is reported. PMID: 27133071
    13. CrkL mediates CCL20/CCR6-induced epithelial-to-mesenchymal transition via Akt pathway, instead of Erk1/2 pathway in development of gastric cancer PMID: 26044596
    14. CrkL regulates CCL19 and CCR7-induced epithelial-to-mesenchymal transition via ERK signaling pathway in epithelial ovarian carcinoma patients. PMID: 25636509
    15. these results suggest that CrkL plays a regulatory role in the SDF-1-induced Erk1/2 and PI3K/Akt pathways and further managed the invasion and migration of breast cancer cells PMID: 25476480
    16. The s show that this potentiation involves reorganization of the natural CrkL-p85beta complex into a novel trimeric complex where influenza A virus NS1 serves as a bridging factor. PMID: 26099693
    17. Our results demonstrate that the p53 target miR-200b/200c/429 miRNAs are negative regulators of the CRKL oncogene PMID: 26079153
    18. results suggested that overexpression of CRKL promoted cell invasion through upregulation of MMP9 expression and activation of ERK pathway PMID: 24664993
    19. CRKL has the potential to be used as a biomarker for the diagnosis, treatment and prognosis of certain tumors PMID: 25531052
    20. We identified ZEB1 and CRKL as potential targets of miR-429 by analyzing combined results from in silico search and global expression array of the same RNA samples. Immunoblot assay confirmed that miR-429 reduced their expression at protein level. PMID: 25405387
    21. These results indicate that CRKL gene amplification is rare in acquisition of resistance to EGFR-TKIs in lung cancer patients with EGFR mutations. PMID: 24939008
    22. CrkL knockdown markedly suppressed the phosphorylated ERK (p-ERK) as well as the phosphorylated AKT (p-AKT) (p < 0.001) compared with control or TGF-b1 alone. PMID: 25307974
    23. our study demonstrated that CRKL was overexpressed in human pancreatic cancers and contributed to pancreatic cancer cell proliferation and invasion through ERK signaling. PMID: 25318601
    24. Study revealed that LASP1 phosphorylation results in an association with CRKL - another specific BCR-ABL substrate and bona fide biomarker for BCR-ABL activity. PMID: 24913448
    25. Haploinsufficiency of CRKL could be responsible for the etiology of conotruncal heart defects in individuals with nested distal deletions and might act as a genetic modifier of individuals with the typical 3 Mb deletion. PMID: 25658046
    26. CrkL is highly expressed in papillary thyroid carcinoma and papillary thyroid microcarcinoma and closely correlated to metastasis. PMID: 25185652
    27. results suggest that CRKL has the ability to regulate colon cancer malignancy and CRKL has the potential to serve as a diagnosis and prognosis marker and a therapy target of colon cancer PMID: 24389200
    28. CRKL is overexpressed in bladder cancer and regulates malignant cell growth and invasion. PMID: 24375195
    29. This is the first report to elucidate the novel function of NS1--binding protein collaborating with viral protein NS1 in modulation of host cell physiology. In addition, an alternative role of adaptor protein CRKL in association with NS1 and NS1-BP during influenza A virus infection is demonstrated. PMID: 24220336
    30. Data show Src-inducible association of CrkL with procaspase-8 promotes cell migration. PMID: 23751956
    31. CRKL may recruit NleH1 to a host kinase on which NleH1 performs its inhibitory function. PMID: 24145029
    32. we identified CRKL/YES as critical interrelated pathways necessary for rhabdomyosarcoma cell growth and survival and suggest a potential therapeutic role of SRC family kinase inhibition in the treatment of rhabdomyosarcoma. PMID: 23318429
    33. Knock-down of CRKL in SGC-7901 cells induced a suppression of cell proliferation along with a significant arrest of cell cycle in G0/G1 phase. PMID: 24055140
    34. Overexpression of CRKL in HBE and H1299 cell lines promoted cell proliferation by facilitating cell cycle progression. PMID: 22753141
    35. High expression levels of the CRKL and CRKL-FLT1 pair strongly correlate with reduced disease-free and overall survival in HCC patient samples. PMID: 23397142
    36. Overexpression of CRKL correlated with progression and malignant proliferation of human breast cancers. PMID: 23686806
    37. SFK activity was shown to be sufficient, but not required for the interaction between ESDN and the CrkL-SH2 domain PMID: 23770091
    38. study reveals a significant association between Crk protein expression with highly proliferative tumors and basal breast cancers of poor outcome; data highlight the physiological importance of Crk proteins in regulating growth of aggressive basal breast cancer cells PMID: 22569336
    39. BCR-ABL activity measured by 50% inhibitory concentration for imatinib, p-CrkL/CrkL ratio or p-CrkL ratio in CD34+ cells of patients with chronic myeloid leukemia does not predict treatment response PMID: 22233112
    40. Stathmin and CrkL proteins may be involved in drug resistance of K562 cells to adriamycin. PMID: 22169288
    41. CRKL overexpression induces cell transformation. PMID: 22586683
    42. These results suggested that CRKL protein is overexpressed in a subset of gastric cancers and is associated with CRKL amplification in gastric cancer. PMID: 22591714
    43. In silico three-dimensional modeling of apoptin, molecular docking experiments between apoptin model and the known structure of Bcr-Abl, and the 3D structures of SH2 domains of CrkL and Bcr-Abl, were performed. PMID: 22253690
    44. these results indicate that CRKL regulates HNSCC-cell growth, motility, and integrin-dependent cell adhesion, suggesting that CRKL plays a principal role in HNSCC tumorigenicity. PMID: 22244889
    45. The molecular signaling set off by ERalpha and CrkL association may have a central role in pregnancy and cancer. PMID: 21700719
    46. Lyn controls spatial activation of Rap1 by recruiting the CrkL-C3G protein complex to the leading edge PMID: 21628423
    47. These results suggest that CRKL, but not MAPK1 is the target oncogene of the rare but recurrent amplification at 22q11.2 in laryngeal squamous cell carcinoma. PMID: 21896986
    48. Findings indicate that amplification and resultant overexpression of CRKL contribute to diverse oncogenic phenotypes in lung cancer. PMID: 19966867
    49. DOCK2 associates with CrkL and regulates Rac1 in human leukemia cell lines PMID: 12393632
    50. directs ASAP1 to peripheral focal adhesions in platelets PMID: 12522101

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  • 蛋白家族：
    CRK family
  • 数据库链接：

    HGNC: 2363

    OMIM: 602007

    KEGG: hsa:1399

    STRING: 9606.ENSP00000346300

    UniGene: Hs.5613