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  4. Recombinant Human Cofilin-1 (CFL1)

Recombinant Human Cofilin-1 (CFL1)

  • 货号：
    CSB-YP005280HU
  • 规格：
  • 来源：
    Yeast
  • 其他：
  • 货号：
    CSB-EP005280HU
  • 规格：
  • 来源：
    E.coli
  • 其他：
  • 货号：
    CSB-EP005280HU-B
  • 规格：
  • 来源：
    E.coli
  • 共轭：
    Avi-tag Biotinylated

    E. coli biotin ligase (BirA) is highly specific in covalently attaching biotin to the 15 amino acid AviTag peptide. This recombinant protein was biotinylated in vivo by AviTag-BirA technology, which method is BriA catalyzes amide linkage between the biotin and the specific lysine of the AviTag.

  • 其他：
  • 货号：
    CSB-BP005280HU
  • 规格：
  • 来源：
    Baculovirus
  • 其他：
  • 货号：
    CSB-MP005280HU
  • 规格：
  • 来源：
    Mammalian cell
  • 其他：

产品详情

  • 纯度：
    >85% (SDS-PAGE)
  • 基因名：
  • Uniprot No.：
  • 别名：
    18 kDa phosphoprotein; CFL 1; CFL; CFL1; COF1_HUMAN; Cofilin 1; Cofilin 1 non muscle; Cofilin; Cofilin non muscle isoform; Cofilin-1; epididymis secretory protein Li 15; HEL-S-15; non-muscle isoform; p18
  • 种属：
    Homo sapiens (Human)
  • 蛋白长度：
    Full Length of Mature Protein
  • 表达区域：
    2-166
  • 氨基酸序列
    ASGVAVSDG VIKVFNDMKV RKSSTPEEVK KRKKAVLFCL SEDKKNIILE EGKEILVGDV GQTVDDPYAT FVKMLPDKDC RYALYDATYE TKESKKEDLV FIFWAPESAP LKSKMIYASS KDAIKKKLTG IKHELQANCY EEVKDRCTLA EKLGGSAVIS LEGKPL
  • 蛋白标签：
    Tag type will be determined during the manufacturing process.
    The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.
  • 产品提供形式：
    Lyophilized powder
    Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
  • 复溶：
    We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
  • 储存条件：
    Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
  • 保质期：
    The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
    Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
  • 货期：
    Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
    Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
  • 注意事项：
    Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
  • Datasheet ：
    Please contact us to get it.

产品评价

靶点详情

  • 功能：
    Binds to F-actin and exhibits pH-sensitive F-actin depolymerizing activity. In conjunction with the subcortical maternal complex (SCMC), plays an essential role for zygotes to progress beyond the first embryonic cell divisions via regulation of actin dynamics. Required for the centralization of the mitotic spindle and symmetric division of zygotes. Plays a role in the regulation of cell morphology and cytoskeletal organization in epithelial cells. Required for the up-regulation of atypical chemokine receptor ACKR2 from endosomal compartment to cell membrane, increasing its efficiency in chemokine uptake and degradation. Required for neural tube morphogenesis and neural crest cell migration.
  • 基因功能参考文献：
    1. In conclusion, deregulation of cytoskeleton dynamics through TESK1/CFL1 pathway underlies epithelial intestinal dysfunction in the small bowel mucosa of diarrhea-predominant irritable bowel syndrome, particularly in female patients. PMID: 29396473
    2. High CFL1 expression is associated with malignant pancreatic lesions. PMID: 29347944
    3. High CFL1 expression is associated with epithelial-mesenchymal transition of gastric cancer. PMID: 28388575
    4. Cofilin-1 plays a dominant role in angiotensin-(1-7)-induced G0/G1 arrest and autophagy to maintain cellular homeostasis in human aortic endothelial cells PMID: 27748441
    5. NKCC1 not only controls cell volume and Cl- concentration, but it can also regulate the actin cytoskeleton through Cofilin 1. PMID: 28679472
    6. The enhancement of cancer cells invasion is dependent on CFL1, a known regulator of invadopodia maturation. PMID: 28446539
    7. The present study demonstrates that miR-145 plays an important role in inhibiting cell migration by directly targeting PAK4, and identifies miR-145-PAK4-LIMK1-cofilin as a novel regulatory pathway that contributed to colorectal cancer metastasis. PMID: 28440035
    8. Our results revealed that CKS1 is involved in normal glutamatergic synapse development and dendritic spine maturation in adult hippocampus through modulating p27 stability. PMID: 28365778
    9. Our study indicates that Cofilin 1 holds an important position in the development and progression of human bladder cancer PMID: 27768223
    10. Results suggest that the immune-complex (IC) of cofilin-1 in sera is a potentially attractive serum biomarker for the prognosis of pancreatic ductal adenocarcinoma (PDAC). PMID: 28161904
    11. High cofilin expression is associated with adrenocortical tumor. PMID: 28826686
    12. In advance urothelial cancer, overexpression of nuclear cofilin correlates with bladder cancer progression. PMID: 27402302
    13. Report structural basis for noncanonical substrate recognition of cofilin-1/LIMK1 to regulation actin cytoskeleton dynamics. PMID: 27153537
    14. STMN1,COF1 and PAIRBP1 thus represent proteins associated with proliferative and aggressive tumors of high grades, while TSP2 and POSTN were connected to low grade tumors with better prognosis PMID: 28216224
    15. We observed marked increases in LIM kinase 2 (LIMK2) and cofilin 1 (CFL1) gene expressions in metabolic syndrome patients. PMID: 26956845
    16. Therefore, miR-138/LIMK1/cofilin may be considered a potential therapeutic target for the treatment of non-small cell lung cancer PMID: 27665963
    17. These findings indicate that inhibition of the Rac1WAVE2Arp2/3 signaling pathway may promote radiosensitivity, which may partially result from the downregulation of CFL1 in U251 human glioma cells. PMID: 27052944
    18. The present findings demonstrated that the anticancer effect of docetaxel induces the apoptosis of prostate cancer via the suppression of the cofilin1 and paxillin signaling pathways, which will assist in setting a stage for the clinical treatment of prostate cancer. PMID: 27035282
    19. VEGFA induced phosphorylation of Pak1 and its effector cofilin in a manner that was dependent on time as well as p38MAPKbeta. PMID: 26857814
    20. CFL1 itself does not translocate actin into the cell nucleus but this transport requires the functional expression of IPO9. PMID: 26934847
    21. Abnormal CFL1 expression may affect vulvar carcinogenesis and subsequent progression. PMID: 26936386
    22. PAK1-cofilin phosphorylation mechanism to mediate lung adenocarcinoma cells migration promoted by apelin-13 PMID: 26918678
    23. Progesterone (P4) increases human astrocytoma cells migration and invasion through its intracellular receptor, and that cofilin activation by P4 is independent of progesterone receptor action. PMID: 26639431
    24. the presence of the actin-severing protein, cofilin, in human sperm, is reported. PMID: 26085344
    25. Aurora kinase A has a role in inducing papillary thyroid cancer lymph node metastasis by promoting cofilin-1 activity PMID: 27003257
    26. analysis of human Cof1, Cof2, and ADF effects on actin filament severing and turnover PMID: 26996939
    27. Cofilin 1 activation prevents the defects in axon elongation and guidance induced by extracellular alpha-synuclein PMID: 26558842
    28. novel roles for actin-depolymerizing factor and cofilin-1 in regulating the remodeling and permeability of epithelial junctions PMID: 26878213
    29. cofilin1 is involved in hypertensive nephropathy by modulating the nuclear translocation of NF-kappaB and the expression of its downstream inflammatory factors in renal tubular epithelial cells PMID: 26450610
    30. Cofilin-1 and transgelin may play roles in the carcinogenesis and development of esophageal squamous cell carcinoma PMID: 26344167
    31. results demonstrate that in VSMCs mechanical stimulation activates cofilin by a Nox4-dependent mechanism and that this pathway is required for cytoskeleton reorganization and cell reorientation PMID: 25998423
    32. Cor1B, Cof1 and AIP1 work in concert through a temporally ordered pathway to induce highly efficient severing and disassembly of actin filaments. PMID: 25995115
    33. the results of the present study suggested that miR-429 inhibits the migration and invasion of colon cancer cells, partly at least, by mediating the expression of PAK6, as well as the activity of cofilin signaling PMID: 26058485
    34. These results indicated that cofilin might be involved in the modulation of Aspergillus fumigatus internalization into type II alveolar epithelial cells through the RhoA-ROCK-LIM kinase pathway. PMID: 26268695
    35. Increasing radiosensitivity with the downregulation of cofilin-1 in U251 human glioma cells PMID: 25529407
    36. estradiol-17beta (E2beta) stimulates S-nitrosylation of CFL1 via eNOS-derived nitric oxide. PMID: 25635941
    37. Chronophin has a role in coordinating cell leading edge dynamics by controlling active cofilin levels PMID: 26324884
    38. cofilin-1 quantification in clinical samples in combination with presence/absence of EGFR mutation could be used to select patients that would benefit from TKI's treatment. PMID: 25784483
    39. Actin-modulating protein cofilin is involved in the formation of measles virus ribonucleoprotein complex at the perinuclear region. PMID: 26269174
    40. Novel signaling pathway consisting of chemoattractant GPCR/Galphai protein, PLC, PKCbeta and PKDs that regulates SSH2/cofilin activity, F-actin polymerization and directional assembly of actin cytoskeleton in neutrophil chemotaxis was identified. PMID: 25568344
    41. Overexpression of wt-cofilin-1 inhibited VEGF-induced F-actin formation PMID: 25056928
    42. Data indicate that knockdown of let-7b or let-7e could recover the growth rate and the invasion of cofilin-1 over-expressing cells. PMID: 25597880
    43. High-speed atomic force microscopy was employed to observe structural changes in actin filaments induced by cofilin binding. PMID: 25642645
    44. Data indicate that gamma-secretase is a negative regulator of Cofilin 1 activation. PMID: 25315299
    45. Actin filament severing by vertebrate cofilin is driven by the linked dissociation of a single, site-specific cation that controls filament structure and mechanical properties, and that filament severing is an essential function of cofilin in cells. PMID: 25468977
    46. ERK5 acted in concert with the actin remodeling protein, CFL1, and upon hormone exposure, both localized to active nuclear transcriptional hubs. PMID: 24505128
    47. We have also defined in the actin-binding protein cofilin-1 a link between PP2A, actin cytoskeleton, and necrotic death PMID: 25096578
    48. It provides a brief overview of the current knowledge about the central role of cofilin/actin rods formation, where its dysregulation and malfunction might be the trigger to neurodegeneration. PMID: 24813767
    49. Brain phosphorylated cofilin is downregulated in transgenic mice by RanBP9 overexpression, accelerating amyloid plaque accumulation. PMID: 24254706
    50. The CFL-1-dependent recruitment of actin to SPCA1 following calcium influx is critical for secretory cargo sorting. PMID: 25179631

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  • 亚细胞定位：
    Nucleus matrix. Cytoplasm, cytoskeleton. Cell projection, ruffle membrane; Peripheral membrane protein; Cytoplasmic side. Cell projection, lamellipodium membrane; Peripheral membrane protein; Cytoplasmic side. Cell projection, lamellipodium. Note=Colocalizes with the actin cytoskeleton in membrane ruffles and lamellipodia. Detected at the cleavage furrow and contractile ring during cytokinesis. Almost completely in nucleus in cells exposed to heat shock or 10% dimethyl sulfoxide.
  • 蛋白家族：
    Actin-binding proteins ADF family
  • 组织特异性：
    Widely distributed in various tissues.
  • 数据库链接：

    HGNC: 1874

    OMIM: 601442

    KEGG: hsa:1072

    STRING: 9606.ENSP00000309629

    UniGene: Hs.170622