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  4. Recombinant Human Caspase-3 (CASP3), partial,Escherichia Coli

Recombinant Human Caspase-3 (CASP3), partial,Escherichia Coli

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  • 货号：
    CSB-EP004548HU
  • 规格：
    ￥1344
  • 图片：
    • (Tris-Glycine gel) Discontinuous SDS-PAGE (reduced) with 5% enrichment gel and 15% separation gel.
    • Based on the SEQUEST from database of E.coli host and target protein, the LC-MS/MS Analysis result of CSB-EP004548HU could indicate that this peptide derived from E.coli-expressed Homo sapiens (Human) CASP3.
    • Based on the SEQUEST from database of E.coli host and target protein, the LC-MS/MS Analysis result of CSB-EP004548HU could indicate that this peptide derived from E.coli-expressed Homo sapiens (Human) CASP3.
  • 其他：

产品详情

  • 纯度：
    >85% (SDS-PAGE)
  • 基因名：
  • Uniprot No.：
  • 别名：
    A830040C14Rik; Apopain; CASP 3; CASP-3; CASP3; CASP3_HUMAN; Casp3a; Caspase 3; Caspase 3; apoptosis-related cysteine peptidase; Caspase 3; apoptosis-related cysteine protease; Caspase 3; apoptosis-related cysteine protease a; Caspase-3 subunit p12; Caspase3; CC3; CPP 32; CPP-32; CPP32; CPP32B; Cysteine protease CPP32; EC 3.4.22.56; ICE3; LICE; mldy; OTTHUMP00000165052; OTTHUMP00000165053; OTTHUMP00000165054; PARP cleavage protease; Procaspase3; Protein Yama; SCA 1; SCA-1; SCA1; SREBP cleavage activity 1; Yama; Yama protein
  • 种属：
    Homo sapiens (Human)
  • 蛋白长度：
    partial
  • 来源：
    E.coli
  • 分子量：
    20.6kDa
  • 表达区域：
    29-175
  • 氨基酸序列
    SG ISLDNSYKMD YPEMGLCIII NNKNFHKSTG MTSRSGTDVD AANLRETFRN LKYEVRNKND LTREEIVELM RDVSKEDHSK RSSFVCVLLS HGEEGIIFGT NGPVDLKKIT NFFRGDRCRS LTGKPKLFII QACRGTELDC GIETD
    Note: The complete sequence including tag sequence, target protein sequence and linker sequence could be provided upon request.
  • 蛋白标签：
    N-terminal 6xHis-tagged
  • 产品提供形式：
    Liquid or Lyophilized powder
    Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
  • 缓冲液：
    Tris-based buffer,50% glycerol
  • 储存条件：
    Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
  • 保质期：
    The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
    Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
  • 货期：
    3-7 business days
  • 注意事项：
    Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
  • 产品描述：

    To prepare this Recombinant Human CASP3 protein, the recombinant DNA was required, which was generated by fusing the CASP3 gene with N-terminal 6xHis tag sequence. Once the recombinant DNA was amplified and purified, a protein expression system, E.coli, was needed for this CASP3 protein production. After purification, a premium CASP3 recombinant proteinwas obtained. According to SDS-PAGE, its purity turns out to be 85%+.

    CASP3 is a gene encoding a protein named caspase-3 (short name is CASP3) or cysteine protease CPP32 (short name is CPP32). CASP3 protein, a member of asparate-specific cysteinyl proteases family, is a small protein consists of two subunits of 12 kDa and 17 kDa. Recently, it has attracted much attention with its incredible roles in tissue differentiation, regeneration, and neural development. This enzyme is a key zymogen in cell apoptosis and is not activated until it is cleaved by initiator caspases during apoptotic flux. Except apoptosis, tt has been found involved in other biological processes, such as anterior neural tube closure, T cell homeostasis and neuron differentiation.

  • Datasheet & COA：
    Please contact us to get it.

产品评价

靶点详情

  • 功能：
    Involved in the activation cascade of caspases responsible for apoptosis execution. At the onset of apoptosis it proteolytically cleaves poly(ADP-ribose) polymerase (PARP) at a '216-Asp-|-Gly-217' bond. Cleaves and activates sterol regulatory element binding proteins (SREBPs) between the basic helix-loop-helix leucine zipper domain and the membrane attachment domain. Cleaves and activates caspase-6, -7 and -9. Involved in the cleavage of huntingtin. Triggers cell adhesion in sympathetic neurons through RET cleavage. Cleaves and inhibits serine/threonine-protein kinase AKT1 in response to oxidative stress. Cleaves XRCC4 and phospholipid scramblase proteins XKR4, XKR8 and XKR9, leading to promote phosphatidylserine exposure on apoptotic cell surface.
  • 基因功能参考文献：
    1. The optimal melatonin concentration (3 mM) significantly decreased the intracellular reactive oxygen species levels, caspase-3 activity and the percentage of both dead and apoptotic-like sperm cells and increased the vitality, progressive motility and total motility and AKT phosphorylation compared with the control group. PMID: 29196809
    2. As phosphorylation of the serine residue of this tetra-peptide could yield a motif similar to the caspase-3 binding recognition sequence DEVD/E, the region from a representative PE_PGRS protein (PE_PGRS45) was docked to human caspase-3. PMID: 30207307
    3. within the modified loop, Ser(150) evolved with the apoptotic caspases, whereas Thr(152) is a more recent evolutionary event in mammalian caspase-3. Substitutions at Ser(150) result in a pH-dependent decrease in dimer stability, and localized changes in the modified loop propagate to the active site of the same protomer through a connecting surface helix. PMID: 29414778
    4. Caspase-3 and -8 and annexin V may serve as diagnostic markers in Ovarian cancer , also explained that the decrement in control of the S phase in the cell cycle may considered one of the significant factors in the development of ovarian tumors PMID: 30197345
    5. Study seems to indicate a direct connection between SNPS in CASP3 gene and prostate cancer (PCa) risk in the Galician population after stratification. Also, individual susceptibility to PCa becomes more evident when assessing gene-environment interactions. Alleles G and T, in rs1049216 and rs2705897 respectively, are related to an increased risk of PCa in smokers overweight individuals. PMID: 30176316
    6. Low CASP3 expression is associated with Colorectal Cancer. PMID: 29801534
    7. overexpressed miR-337-3p and miR-17-5p/miR-132-3p/-212-3p can regulate executioner caspases-3 and -7, respectively. PMID: 29659498
    8. Caspase-8 and Caspase-3 expressions in tumor tissues are novel candidate prognostic markers for colorectal cancer patients PMID: 29355114
    9. The new findings of this work were that an association between serum caspase-3 concentrations during the first week, apoptosis degree, sepsis severity, and sepsis mortality exists. PMID: 29119350
    10. our data demonstrate that WT1 protein undergoes proteolytic processing by caspase-3 in chemotherapeutic drugs-induced apoptosis. This processing is associated with a reduction of WT1 protein. PMID: 28395566
    11. Increased baseline gene expressions of RUNX2, p21 and caspase 3 in the peripheral blood might predict better responses to methotrexate therapy. PMID: 28741869
    12. The caspase-3-mediated movement of PUS10 and the release of mitochondrial contents enhancing caspase-3 activity creates a feedback amplification loop for caspase-3 action. Therefore, any defect in the movement or interactions of PUS10 would reduce the TRAIL sensitivity of tumor cells. PMID: 28981101
    13. a prolonged anti-apoptotic intervention targeting caspase-3 should be considered with caution due to the potential adverse effects in mitochondria dynamics resulting from a novel potential functional role of procaspase-3 in mitochondrial biogenesis via regulating the expression of mitochondrial biogenesis activators. PMID: 28585712
    14. knockdown of RPA1 suppressed cell clone formation, induced cell cycle arrest at the G1 phase and promoted cell apoptosis by regulating the protein level of Caspase 3 PMID: 29601890
    15. The phosphorylation level of p38 was upregulated by MA1 treatment, and the inhibitor of p38, SB203580, attenuated the MA1-induced p38 phosphorylation as well as caspase3 and PARP activation. These results indicate that MA1 treatment alters invasive and oncogenic phenotypes of human colorectal cancer cells through the stimulation of the p38 signaling pathway PMID: 28713983
    16. Overexpression of full-length AIFM1 suppresses proliferation and induces apoptosis of HepG2 and Hep3B cells. Caspase 3 and DRAM are involved in full-length AIFM1-induced apoptosis in HepG2 and Hep3B cells. PMID: 29501488
    17. Here the s show that sublethal activation of Caspase-3 plays an essential, facilitative role in Myc-induced genomic instability and oncogenic transformation. PMID: 28691902
    18. we show that ABT-737 and TQ activate PKA in a caspase-3-dependent manner, which correlates with platelet inhibition and apoptosis and therefore potentially contributes to the bleeding risk in chemotherapy patients PMID: 28661475
    19. MiR-221 might represent a candidate biomarker of likelihood of response to Sorafenib in HCC patients to be tested in future studies. Caspase-3 modulation by miR-221 participates to Sorafenib resistance PMID: 28096271
    20. In the present study, galangin was found to suppress laryngeal cancer cell proliferation. Also, flow cytometry, immunohistochemical and western blot analysis indicated that cell apoptosis was induced for galangin administration, promoting caspase-3 expression through regulating PI3K/AKT/NF-kappaB. PMID: 28677816
    21. 1,4-BQ evidently induced mitochondria-mediated apoptosis and increased pro-apoptotic genes (Caspase-9 and Caspase-3) expression in a dose-dependent manner. PMID: 27425441
    22. GGN played a tumor-promoting role in bladder cancer through regulation of NFkappaB/caspase3-mediated apoptosis signaling. PMID: 29412153
    23. Serum caspase-3 concentrations are increased in ICH patients as well as correlate with clinical severity and prognosis PMID: 28526532
    24. High caspase-3 expression is significantly associated with adverse breast cancer-specific survival. High caspase-3 expression was significantly associated with HER2 positive tumours.The prognostic significance of caspase-3 expression in different breast cancer phenotypes was also examined.There was a significant association in receptor positive (ER, PR or HER2) and non-basal like subgroups. PMID: 27798717
    25. UV phototoxicity-induced pre-elafin inside keratinocytes prior to cornified envelope formation could be involved in UV-induced keratinocyte apoptosis via cystatin-A downregulation resulting in pro-caspase-3 activation. PMID: 28119996
    26. Overexpression of CASP3 is associated with Breast Cancer. PMID: 26932709
    27. Results show that CASP3 expression is regulated by HOXC13 which represses its transcription by directly targeting its promotor region. PMID: 29168599
    28. Data show that selective histone deacetylase 6 (HDAC6) inhibition or knockdown of HDAC6 expression was able to prevent caspase 3 activation in lung endothelial cells and maintain lung endothelial cell-cell junctions. PMID: 27419634
    29. Genetic variations in the CASP3 gene and the joint effects of working time and CASP3 polymorphisms may modify the risk of developing noise-induced hearing loss PMID: 28738811
    30. Data indicate that through upregulating the expression of caspase-3, the TT genotype of caspase-3 rs1049216 can be associated with not only the risk of cervical cancer but also the progression of this cancer. PMID: 28114230
    31. In conclusion, our findings firstly revealed that GSDME switches chemotherapy drug-induced caspase-3 dependent apoptosis into pyroptosis in gastric cancer cells. PMID: 29183726
    32. Everolimus also induced higher levels of caspase-3/-7 activation in GR over GS cells, and everolimus-mediated mTOR inhibition lead to G2 arrest in GR cells but G1 arrest in GS cells. PMID: 28165150
    33. Results Grb7 and Hax1 may colocalize partially to mitochondria in EGF-treated SKBR3 cells and their interaction can affect Caspase3 cleavage of Hax1 supporting an inhibitory role of Grb7 on Casp3 cleavage function by interfering with the association of Casp3 and Hax1. PMID: 26869103
    34. caspase-3 inhibitors also suppressed the attenuation of cell adhesion and phosphorylation of p38 MAPK by EGF-F9. Our data indicated that EGF-F9 activated signals for apoptosis and induced de-adhesion in a caspase-3 dependent manner. PMID: 27129300
    35. Data indicate that E-cadherin and caspase-3 were targets of miR-421, which was up-regulated by HIF-1alpha. PMID: 27016414
    36. findings suggest that caspase-3 activation can trigger necrosis by cleaving GSDME and offer new insights into cancer chemotherapy PMID: 28459430
    37. These results demonstrate that hyperglycemic-induced endothelial microparticles increase endothelial cell active caspase-3. This apoptotic effect may be mediated, at least in part, by a reduction in miR-Let-7a expression. PMID: 28942148
    38. Epigallocatechin-3-Gallate protects against Ang II-induced HUVEC apoptosis by decreasing oxidative stress and ameliorating mitochondrial injury via activation of Nrf2/casp3 signaling pathway. PMID: 28942440
    39. Prolonged treatment of human PMNs or mice bone marrow derived neutrophils (BMDN) with nitric oxide led to enhanced reactive oxygen species generation, caspase-8/caspase-3 cleavage, reduced mitochondrial membrane potential and finally cellular apoptosis. PMID: 27584786
    40. cleaved caspase-3 and caspase-3/8/9 could be biomarkers for tumorigenesis in oral tongue squamous cell carcinoma patients. PMID: 28700659
    41. TT genotype of CASP3 rs4643701 polymorphisms showed risk in CAD. CASP3 rs4647601 creates a new exon splicing enhancer. PMID: 28633917
    42. These findings shed some light on how a tumor cell may avert apoptosis using Hsp60 and point to the anti-cancer potential of drugs, such as CubipyOXA, which interfere with Hsp60/pC3 complex formation, and thus allow the apoptotic cascade to proceed. PMID: 28212901
    43. Here the s show that in macrophages SipA induces increased caspase-3 activation early in infection. PMID: 28630067
    44. SASH1 is cleaved by caspase-3 following Ultraviolet C-induced apoptosis. PMID: 27831555
    45. Caspase 3 activation in dying glioma cells unfavorably supported post-irradiation angiogenesis. PMID: 27826040
    46. CASP3 is a direct target of specific Epstein-Barr virus BART miRNAs. PMID: 27565721
    47. Data suggest that EV71 infection in enterocytes does not inhibit phosphorylation of STAT1/2 induced by IFN-beta, but p-STAT1/2 transport into the nucleus is significantly blocked; EV71 infection in enterocytes down-regulates expression of KPNA1 and induces degradation of cellular KPNA1 via caspase-3. [EV17 = Enterovirus 71] PMID: 28455446
    48. our results identified that mammalian sterile 20-like kinase 1 is a novel downstream target of pyruvate kinase M2, and knockdown of pyruvate kinase M2 contributes apoptosis via promoting nuclear translocation of mammalian sterile 20-like kinase 1 by enhancing Caspase-3-dependent cleavage. PMID: 28656802
    49. High levels of FADD and caspase-8, but not caspase-3, were associated with increased incidence of coronary events in subjects from the general population. PMID: 28302628
    50. Interestingly, EspC-induced apoptosis was triggered through a dual mechanism involving both independent and dependent functions of its EspC serine protease motif, the direct cleavage of procaspase-3 being dependent on this motif. PMID: 27329750

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  • 亚细胞定位：
    Cytoplasm.
  • 蛋白家族：
    Peptidase C14A family
  • 组织特异性：
    Highly expressed in lung, spleen, heart, liver and kidney. Moderate levels in brain and skeletal muscle, and low in testis. Also found in many cell lines, highest expression in cells of the immune system.
  • 数据库链接：

    HGNC: 1504

    OMIM: 600636

    KEGG: hsa:836

    STRING: 9606.ENSP00000311032

    UniGene: Hs.141125