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  4. Human Caspase 3,Casp-3 ELISA Kit

Human Caspase 3,Casp-3 ELISA Kit

  • 中文名称：
    人胱天蛋白酶3(Casp-3)酶联免疫试剂盒
  • 货号：
    CSB-E08856h
  • 规格：
    96T/48T
  • 价格：
    ￥3200/￥2500
  • 其他：

产品详情

  • 产品描述：

    The Human CASP3 ELISA Kit allows for the in vitro quantitative determination of CASP3 concentrations in serum, plasma, cell lysates, or tissue homogenates. This kit exclusively recognizes human CASP3. CASP3 is a frequently activated death protease and is responsible for the proteolytic cleavage of many specific target proteins leading to typical morphological changes in apoptotic cells. Studies have shown that CASP3 induced apoptosis in neurons during programmed developmental cell death and that CASP3 is required for normal brain development. CASP3-induced apoptosis has also been observed in microglia and astrocytes of developing brains. In addition to its apoptotic functions, CASP3 has also been demonstrated to influence the survival, proliferation, and differentiation of both normal and malignant cells and tissues. CASP3 is also necessary for the development of the auditory function.

    The detection mechanism of this kit is based on the Sandwich-ELISA technique. CASP3 in the sample is bound to the capture antibody immobilized on the microtiter plate and then sandwiched with a Biotin-labeled CASP3 antibody. The solution color develops into blue after the ordinal addition of HRP-avidin and TMB. The color development is terminated after adding the stop solution, and the color turns from blue to yellow. The color intensity is positively correlated with CASP3 content in the sample. The kit has been quality-controlled with high sensitivity, strong specificity, good linearity, high precision, high recovery, and high lot-to-lot consistency.

  • 别名：
    A830040C14Rik ELISA Kit; Apopain ELISA Kit; CASP 3 ELISA Kit; CASP-3 ELISA Kit; CASP3 ELISA Kit; CASP3_HUMAN ELISA Kit; Casp3a ELISA Kit; Caspase 3 ELISA Kit; Caspase 3; apoptosis-related cysteine peptidase ELISA Kit; Caspase 3; apoptosis-related cysteine protease ELISA Kit; Caspase 3; apoptosis-related cysteine protease a ELISA Kit; Caspase-3 subunit p12 ELISA Kit; Caspase3 ELISA Kit; CC3 ELISA Kit; CPP 32 ELISA Kit; CPP-32 ELISA Kit; CPP32 ELISA Kit; CPP32B ELISA Kit; Cysteine protease CPP32 ELISA Kit; EC 3.4.22.56 ELISA Kit; ICE3 ELISA Kit; LICE ELISA Kit; mldy ELISA Kit; OTTHUMP00000165052 ELISA Kit; OTTHUMP00000165053 ELISA Kit; OTTHUMP00000165054 ELISA Kit; PARP cleavage protease ELISA Kit; Procaspase3 ELISA Kit; Protein Yama ELISA Kit; SCA 1 ELISA Kit; SCA-1 ELISA Kit; SCA1 ELISA Kit; SREBP cleavage activity 1 ELISA Kit; Yama ELISA Kit; Yama protein ELISA Kit
  • 缩写：
  • Uniprot No.：
  • 种属：
    Homo sapiens (Human)
  • 样本类型：
    serum, plasma, cell lysates, tissue homogenates
  • 检测范围：
    0.312 ng/mL-20 ng/mL
  • 灵敏度：
    0.078 ng/mL
  • 反应时间：
    1-5h
  • 样本体积：
    50-100ul
  • 检测波长：
    450 nm
  • 研究领域：
    Cell Biology
  • 测定原理：
    quantitative
  • 测定方法：
    Sandwich
  • 精密度：
    Intra-assay Precision (Precision within an assay): CV%<8%      
    Three samples of known concentration were tested twenty times on one plate to assess.  
    Inter-assay Precision (Precision between assays): CV%<10%      
    Three samples of known concentration were tested in twenty assays to assess.    
                 
  • 线性度：
    To assess the linearity of the assay, samples were spiked with high concentrations of human Casp-3 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
    　 Sample Serum(n=4)  
    1:1 Average % 94  
    Range % 90-101  
    1:2 Average % 94  
    Range % 83-101  
    1:4 Average % 96  
    Range % 90-106  
    1:8 Average % 94  
    Range % 85-105  
  • 回收率：
    The recovery of human Casp-3 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.
    Sample Type Average % Recovery Range  
    Serum (n=5) 95 89-104  
    EDTA plasma (n=4) 96 91-100  
                 
                 
  • 标准曲线：
    These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.
    ng/ml OD1 OD2 Average Corrected  
    20 2.758 2.762 2.760 2.653  
    10 2.476 2.461 2.469 2.362  
    5 2.002 1.902 1.952 1.845  
    2.5 1.245 1.209 1.227 1.120  
    1.25 0.622 0.612 0.617 0.510  
    0.625 0.308 0.325 0.317 0.210  
    0.312 0.212 0.224 0.218 0.111  
    0 0.105 0.109 0.107 　  
  • 数据处理：
  • 货期：
    3-5 working days

引用文献

产品评价

靶点详情

  • 功能：
    Involved in the activation cascade of caspases responsible for apoptosis execution. At the onset of apoptosis it proteolytically cleaves poly(ADP-ribose) polymerase (PARP) at a '216-Asp-|-Gly-217' bond. Cleaves and activates sterol regulatory element binding proteins (SREBPs) between the basic helix-loop-helix leucine zipper domain and the membrane attachment domain. Cleaves and activates caspase-6, -7 and -9. Involved in the cleavage of huntingtin. Triggers cell adhesion in sympathetic neurons through RET cleavage. Cleaves and inhibits serine/threonine-protein kinase AKT1 in response to oxidative stress. Cleaves XRCC4 and phospholipid scramblase proteins XKR4, XKR8 and XKR9, leading to promote phosphatidylserine exposure on apoptotic cell surface.
  • 基因功能参考文献：
    1. The optimal melatonin concentration (3 mM) significantly decreased the intracellular reactive oxygen species levels, caspase-3 activity and the percentage of both dead and apoptotic-like sperm cells and increased the vitality, progressive motility and total motility and AKT phosphorylation compared with the control group. PMID: 29196809
    2. As phosphorylation of the serine residue of this tetra-peptide could yield a motif similar to the caspase-3 binding recognition sequence DEVD/E, the region from a representative PE_PGRS protein (PE_PGRS45) was docked to human caspase-3. PMID: 30207307
    3. within the modified loop, Ser(150) evolved with the apoptotic caspases, whereas Thr(152) is a more recent evolutionary event in mammalian caspase-3. Substitutions at Ser(150) result in a pH-dependent decrease in dimer stability, and localized changes in the modified loop propagate to the active site of the same protomer through a connecting surface helix. PMID: 29414778
    4. Caspase-3 and -8 and annexin V may serve as diagnostic markers in Ovarian cancer , also explained that the decrement in control of the S phase in the cell cycle may considered one of the significant factors in the development of ovarian tumors PMID: 30197345
    5. Study seems to indicate a direct connection between SNPS in CASP3 gene and prostate cancer (PCa) risk in the Galician population after stratification. Also, individual susceptibility to PCa becomes more evident when assessing gene-environment interactions. Alleles G and T, in rs1049216 and rs2705897 respectively, are related to an increased risk of PCa in smokers overweight individuals. PMID: 30176316
    6. Low CASP3 expression is associated with Colorectal Cancer. PMID: 29801534
    7. overexpressed miR-337-3p and miR-17-5p/miR-132-3p/-212-3p can regulate executioner caspases-3 and -7, respectively. PMID: 29659498
    8. Caspase-8 and Caspase-3 expressions in tumor tissues are novel candidate prognostic markers for colorectal cancer patients PMID: 29355114
    9. The new findings of this work were that an association between serum caspase-3 concentrations during the first week, apoptosis degree, sepsis severity, and sepsis mortality exists. PMID: 29119350
    10. our data demonstrate that WT1 protein undergoes proteolytic processing by caspase-3 in chemotherapeutic drugs-induced apoptosis. This processing is associated with a reduction of WT1 protein. PMID: 28395566
    11. Increased baseline gene expressions of RUNX2, p21 and caspase 3 in the peripheral blood might predict better responses to methotrexate therapy. PMID: 28741869
    12. The caspase-3-mediated movement of PUS10 and the release of mitochondrial contents enhancing caspase-3 activity creates a feedback amplification loop for caspase-3 action. Therefore, any defect in the movement or interactions of PUS10 would reduce the TRAIL sensitivity of tumor cells. PMID: 28981101
    13. a prolonged anti-apoptotic intervention targeting caspase-3 should be considered with caution due to the potential adverse effects in mitochondria dynamics resulting from a novel potential functional role of procaspase-3 in mitochondrial biogenesis via regulating the expression of mitochondrial biogenesis activators. PMID: 28585712
    14. knockdown of RPA1 suppressed cell clone formation, induced cell cycle arrest at the G1 phase and promoted cell apoptosis by regulating the protein level of Caspase 3 PMID: 29601890
    15. The phosphorylation level of p38 was upregulated by MA1 treatment, and the inhibitor of p38, SB203580, attenuated the MA1-induced p38 phosphorylation as well as caspase3 and PARP activation. These results indicate that MA1 treatment alters invasive and oncogenic phenotypes of human colorectal cancer cells through the stimulation of the p38 signaling pathway PMID: 28713983
    16. Overexpression of full-length AIFM1 suppresses proliferation and induces apoptosis of HepG2 and Hep3B cells. Caspase 3 and DRAM are involved in full-length AIFM1-induced apoptosis in HepG2 and Hep3B cells. PMID: 29501488
    17. Here the s show that sublethal activation of Caspase-3 plays an essential, facilitative role in Myc-induced genomic instability and oncogenic transformation. PMID: 28691902
    18. we show that ABT-737 and TQ activate PKA in a caspase-3-dependent manner, which correlates with platelet inhibition and apoptosis and therefore potentially contributes to the bleeding risk in chemotherapy patients PMID: 28661475
    19. MiR-221 might represent a candidate biomarker of likelihood of response to Sorafenib in HCC patients to be tested in future studies. Caspase-3 modulation by miR-221 participates to Sorafenib resistance PMID: 28096271
    20. In the present study, galangin was found to suppress laryngeal cancer cell proliferation. Also, flow cytometry, immunohistochemical and western blot analysis indicated that cell apoptosis was induced for galangin administration, promoting caspase-3 expression through regulating PI3K/AKT/NF-kappaB. PMID: 28677816
    21. 1,4-BQ evidently induced mitochondria-mediated apoptosis and increased pro-apoptotic genes (Caspase-9 and Caspase-3) expression in a dose-dependent manner. PMID: 27425441
    22. GGN played a tumor-promoting role in bladder cancer through regulation of NFkappaB/caspase3-mediated apoptosis signaling. PMID: 29412153
    23. Serum caspase-3 concentrations are increased in ICH patients as well as correlate with clinical severity and prognosis PMID: 28526532
    24. High caspase-3 expression is significantly associated with adverse breast cancer-specific survival. High caspase-3 expression was significantly associated with HER2 positive tumours.The prognostic significance of caspase-3 expression in different breast cancer phenotypes was also examined.There was a significant association in receptor positive (ER, PR or HER2) and non-basal like subgroups. PMID: 27798717
    25. UV phototoxicity-induced pre-elafin inside keratinocytes prior to cornified envelope formation could be involved in UV-induced keratinocyte apoptosis via cystatin-A downregulation resulting in pro-caspase-3 activation. PMID: 28119996
    26. Overexpression of CASP3 is associated with Breast Cancer. PMID: 26932709
    27. Results show that CASP3 expression is regulated by HOXC13 which represses its transcription by directly targeting its promotor region. PMID: 29168599
    28. Data show that selective histone deacetylase 6 (HDAC6) inhibition or knockdown of HDAC6 expression was able to prevent caspase 3 activation in lung endothelial cells and maintain lung endothelial cell-cell junctions. PMID: 27419634
    29. Genetic variations in the CASP3 gene and the joint effects of working time and CASP3 polymorphisms may modify the risk of developing noise-induced hearing loss PMID: 28738811
    30. Data indicate that through upregulating the expression of caspase-3, the TT genotype of caspase-3 rs1049216 can be associated with not only the risk of cervical cancer but also the progression of this cancer. PMID: 28114230
    31. In conclusion, our findings firstly revealed that GSDME switches chemotherapy drug-induced caspase-3 dependent apoptosis into pyroptosis in gastric cancer cells. PMID: 29183726
    32. Everolimus also induced higher levels of caspase-3/-7 activation in GR over GS cells, and everolimus-mediated mTOR inhibition lead to G2 arrest in GR cells but G1 arrest in GS cells. PMID: 28165150
    33. Results Grb7 and Hax1 may colocalize partially to mitochondria in EGF-treated SKBR3 cells and their interaction can affect Caspase3 cleavage of Hax1 supporting an inhibitory role of Grb7 on Casp3 cleavage function by interfering with the association of Casp3 and Hax1. PMID: 26869103
    34. caspase-3 inhibitors also suppressed the attenuation of cell adhesion and phosphorylation of p38 MAPK by EGF-F9. Our data indicated that EGF-F9 activated signals for apoptosis and induced de-adhesion in a caspase-3 dependent manner. PMID: 27129300
    35. Data indicate that E-cadherin and caspase-3 were targets of miR-421, which was up-regulated by HIF-1alpha. PMID: 27016414
    36. findings suggest that caspase-3 activation can trigger necrosis by cleaving GSDME and offer new insights into cancer chemotherapy PMID: 28459430
    37. These results demonstrate that hyperglycemic-induced endothelial microparticles increase endothelial cell active caspase-3. This apoptotic effect may be mediated, at least in part, by a reduction in miR-Let-7a expression. PMID: 28942148
    38. Epigallocatechin-3-Gallate protects against Ang II-induced HUVEC apoptosis by decreasing oxidative stress and ameliorating mitochondrial injury via activation of Nrf2/casp3 signaling pathway. PMID: 28942440
    39. Prolonged treatment of human PMNs or mice bone marrow derived neutrophils (BMDN) with nitric oxide led to enhanced reactive oxygen species generation, caspase-8/caspase-3 cleavage, reduced mitochondrial membrane potential and finally cellular apoptosis. PMID: 27584786
    40. cleaved caspase-3 and caspase-3/8/9 could be biomarkers for tumorigenesis in oral tongue squamous cell carcinoma patients. PMID: 28700659
    41. TT genotype of CASP3 rs4643701 polymorphisms showed risk in CAD. CASP3 rs4647601 creates a new exon splicing enhancer. PMID: 28633917
    42. These findings shed some light on how a tumor cell may avert apoptosis using Hsp60 and point to the anti-cancer potential of drugs, such as CubipyOXA, which interfere with Hsp60/pC3 complex formation, and thus allow the apoptotic cascade to proceed. PMID: 28212901
    43. Here the s show that in macrophages SipA induces increased caspase-3 activation early in infection. PMID: 28630067
    44. SASH1 is cleaved by caspase-3 following Ultraviolet C-induced apoptosis. PMID: 27831555
    45. Caspase 3 activation in dying glioma cells unfavorably supported post-irradiation angiogenesis. PMID: 27826040
    46. CASP3 is a direct target of specific Epstein-Barr virus BART miRNAs. PMID: 27565721
    47. Data suggest that EV71 infection in enterocytes does not inhibit phosphorylation of STAT1/2 induced by IFN-beta, but p-STAT1/2 transport into the nucleus is significantly blocked; EV71 infection in enterocytes down-regulates expression of KPNA1 and induces degradation of cellular KPNA1 via caspase-3. [EV17 = Enterovirus 71] PMID: 28455446
    48. our results identified that mammalian sterile 20-like kinase 1 is a novel downstream target of pyruvate kinase M2, and knockdown of pyruvate kinase M2 contributes apoptosis via promoting nuclear translocation of mammalian sterile 20-like kinase 1 by enhancing Caspase-3-dependent cleavage. PMID: 28656802
    49. High levels of FADD and caspase-8, but not caspase-3, were associated with increased incidence of coronary events in subjects from the general population. PMID: 28302628
    50. Interestingly, EspC-induced apoptosis was triggered through a dual mechanism involving both independent and dependent functions of its EspC serine protease motif, the direct cleavage of procaspase-3 being dependent on this motif. PMID: 27329750

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  • 亚细胞定位：
    Cytoplasm.
  • 蛋白家族：
    Peptidase C14A family
  • 组织特异性：
    Highly expressed in lung, spleen, heart, liver and kidney. Moderate levels in brain and skeletal muscle, and low in testis. Also found in many cell lines, highest expression in cells of the immune system.
  • 数据库链接：

    HGNC: 1504

    OMIM: 600636

    KEGG: hsa:836

    STRING: 9606.ENSP00000311032

    UniGene: Hs.141125