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  4. Recombinant Human Borealin (CDCA8)

Recombinant Human Borealin (CDCA8)

  • 货号：
    CSB-YP700653HU
  • 规格：
  • 来源：
    Yeast
  • 其他：
  • 货号：
    CSB-EP700653HU
  • 规格：
  • 来源：
    E.coli
  • 其他：
  • 货号：
    CSB-EP700653HU-B
  • 规格：
  • 来源：
    E.coli
  • 共轭：
    Avi-tag Biotinylated

    E. coli biotin ligase (BirA) is highly specific in covalently attaching biotin to the 15 amino acid AviTag peptide. This recombinant protein was biotinylated in vivo by AviTag-BirA technology, which method is BriA catalyzes amide linkage between the biotin and the specific lysine of the AviTag.

  • 其他：
  • 货号：
    CSB-BP700653HU
  • 规格：
  • 来源：
    Baculovirus
  • 其他：
  • 货号：
    CSB-MP700653HU
  • 规格：
  • 来源：
    Mammalian cell
  • 其他：

产品详情

  • 纯度：
    >85% (SDS-PAGE)
  • 基因名：
    CDCA8
  • Uniprot No.：
  • 别名：
    BOR; BOREA_HUMAN; Borealin; CDCA8; Cell division cycle associated 8; cell division cycle associated protein 8; Cell division cycle-associated protein 8; Dasra B; Dasra-B; DasraB; FLJ10468; FLJ12042; hDasra B; hDasra-B; MESRGP; OTTHUMP00000004514; pluripotent embryonic stem cell related gene 3 protein; Pluripotent embryonic stem cell-related gene 3 protein
  • 种属：
    Homo sapiens (Human)
  • 蛋白长度：
    full length protein
  • 表达区域：
    1-280
  • 氨基酸序列
    MAPRKGSSRV AKTNSLRRRK LASFLKDFDR EVEIRIKQIE SDRQNLLKEV DNLYNIEILR LPKALREMNW LDYFALGGNK QALEEAATAD LDITEINKLT AEAIQTPLKS AKTRKVIQVD EMIVEEEEEE ENERKNLQTA RVKRCPPSKK RTQSIQGKGK GKRSSRANTV TPAVGRLEVS MVKPTPGLTP RFDSRVFKTP GLRTPAAGER IYNISGNGSP LADSKEIFLT VPVGGGESLR LLASDLQRHS IAQLDPEALG NIKKLSNRLA QICSSIRTHK
  • 蛋白标签：
    Tag type will be determined during the manufacturing process.
    The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.
  • 产品提供形式：
    Lyophilized powder
    Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
  • 复溶：
    We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
  • 储存条件：
    Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
  • 保质期：
    The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
    Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
  • 货期：
    Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
    Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
  • 注意事项：
    Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
  • Datasheet ：
    Please contact us to get it.

产品评价

靶点详情

  • 功能：
    Component of the chromosomal passenger complex (CPC), a complex that acts as a key regulator of mitosis. The CPC complex has essential functions at the centromere in ensuring correct chromosome alignment and segregation and is required for chromatin-induced microtubule stabilization and spindle assembly. Major effector of the TTK kinase in the control of attachment-error-correction and chromosome alignment.
  • 基因功能参考文献：
    1. Results show that CDCA8 is overexpressed in bladder cancer (BC) and its high levels are correlated with poor clinicopathological features of BC patients. Therefore, CDCA8 may act as a novel prognostic marker in the diagnosis of patients with BC. PMID: 30142792
    2. These results were well correlated with the same gene expression pattern analysed in the thyroid tissue of the patient with BOREALIN-p.R114W. These studies open new avenues in the genetics of TD in humans. PMID: 28025328
    3. Borealin dimerization mediates chromosomal passenger complex checkpoint function by enhancing localization to centromeres and kinetochores. PMID: 25854549
    4. enhanced CDCA8 promoter activities by NF-Y overexpression and reduced CDCA8 transcription by NF-Y knockdown further verified that NF-Y is a positive regulator of CDCA8 transcription. PMID: 26170459
    5. In colorectal cancer patients, Borealin expression was positively correlated with age, lymph node metastasis and neoplasm staging. PMID: 25260804
    6. These findings reveal a previously unrecognized but direct link between HP1 and CPC localization in the centromere and illustrate the critical role of borealin-HP1 interaction in orchestrating an accurate cell division. PMID: 24917673
    7. Borealin interacts directly with the Snf7 components of ESCRT-III in human cells.the Borealin central region encompassing residues 110-207 was both necessary & sufficient to bind to CHMP4C. PMID: 22724069
    8. MLL5 functionally interacts with Borealin, facilitates the expression of chromosomal passenger complex, and hence contributes to mitotic fidelity and genomic integrity. PMID: 22797924
    9. important role for phosphorylation of Borealin at S219 in the proper progression through mitosis PMID: 20803554
    10. Data show that INCENP has an important role in stabilizing the chromosomal passenger complex, and that Borealin acts to promote binding of Survivin to INCENP. PMID: 16239925
    11. Cell cycle-regulated chromosomal passenger protein whose aberrant expression and nuclear accumulation are linked to poor prognosis for gastric cancer. PMID: 16427043
    12. A functional module within the chromosomal passenger complex involving the inner centromere protein INCENP, Survivin, and Borealin. PMID: 16571674
    13. Borealin is phosphorylated during mitosis. Neither residue S165, T106 nor phosphorylation of borealin by Aurora B Kinase is required to generate the mitotic, shifted form of Borealin. PMID: 17241471
    14. Suppression of CDCA8 expression with small interfering RNA against CDCA8 significantly suppressed the growth of lung cancer cells. PMID: 17483322
    15. It is recorded that borealin is a cell cycle regulator, down-regulated in response to p53/Rb-signaling, and up-regulated in many types of cancerous tissues. PMID: 17716930
    16. mitotic regulator Survivin binds as a monomer to its functional interactor Borealin PMID: 17881355
    17. Borealin and INCENP associate with the helical domain of Survivin to form a tight three-helical bundle. PMID: 17956729
    18. Borealin plays a crucial role in the early mouse embryonic development PMID: 18311593
    19. Data describe a mitotic SUMO2/3 conjugation-deconjugation cycle of Borealin and further assign a regulatory function of RanBP2 and SENP3 in the mitotic SUMO pathway. PMID: 18946085
    20. found that substitutions at Borealin T230, recently identified as an Mps1 phosphorylation site, can modulate the dimerization state of Borealin PMID: 19530738

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  • 亚细胞定位：
    Nucleus, nucleolus. Cytoplasm. Cytoplasm, cytoskeleton, spindle. Chromosome, centromere.
  • 蛋白家族：
    Borealin family
  • 数据库链接：

    HGNC: 14629

    OMIM: 609977

    KEGG: hsa:55143

    STRING: 9606.ENSP00000316121

    UniGene: Hs.524571