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  4. Recombinant Human Acid sphingomyelinase-like phosphodiesterase 3a (SMPDL3A)

Recombinant Human Acid sphingomyelinase-like phosphodiesterase 3a (SMPDL3A)

  • 中文名称：
    人SMPDL3A重组蛋白
  • 货号：
    CSB-YP842119HU
  • 规格：
  • 来源：
    Yeast
  • 其他：
  • 中文名称：
    人SMPDL3A重组蛋白
  • 货号：
    CSB-EP842119HU
  • 规格：
  • 来源：
    E.coli
  • 其他：
  • 中文名称：
    人SMPDL3A重组蛋白
  • 货号：
    CSB-EP842119HU-B
  • 规格：
  • 来源：
    E.coli
  • 共轭：
    Avi-tag Biotinylated

    E. coli biotin ligase (BirA) is highly specific in covalently attaching biotin to the 15 amino acid AviTag peptide. This recombinant protein was biotinylated in vivo by AviTag-BirA technology, which method is BriA catalyzes amide linkage between the biotin and the specific lysine of the AviTag.

  • 其他：
  • 中文名称：
    人SMPDL3A重组蛋白
  • 货号：
    CSB-BP842119HU
  • 规格：
  • 来源：
    Baculovirus
  • 其他：
  • 中文名称：
    人SMPDL3A重组蛋白
  • 货号：
    CSB-MP842119HU
  • 规格：
  • 来源：
    Mammalian cell
  • 其他：

产品详情

  • 纯度：
    >85% (SDS-PAGE)
  • 基因名：
    SMPDL3A
  • Uniprot No.：
  • 别名：
    SMPDL3A; ASML3AAcid sphingomyelinase-like phosphodiesterase 3a; ASM-like phosphodiesterase 3a; EC 3.1.4.-
  • 种属：
    Homo sapiens (Human)
  • 蛋白长度：
    Full Length of Mature Protein
  • 表达区域：
    23-453
  • 氨基酸序列
    LPVAPAGG RNPPPAIGQF WHVTDLHLDP TYHITDDHTK VCASSKGANA SNPGPFGDVL CDSPYQLILS AFDFIKNSGQ EASFMIWTGD SPPHVPVPEL STDTVINVIT NMTTTIQSLF PNLQVFPALG NHDYWPQDQL PVVTSKVYNA VANLWKPWLD EEAISTLRKG GFYSQKVTTN PNLRIISLNT NLYYGPNIMT LNKTDPANQF EWLESTLNNS QQNKEKVYII AHVPVGYLPS SQNITAMREY YNEKLIDIFQ KYSDVIAGQF YGHTHRDSIM VLSDKKGSPV NSLFVAPAVT PVKSVLEKQT NNPGIRLFQY DPRDYKLLDM LQYYLNLTEA NLKGESIWKL EYILTQTYDI EDLQPESLYG LAKQFTILDS KQFIKYYNYF FVSYDSSVTC DKTCKAFQIC AIMNLDNISY ADCLKQLYIK HNY
  • 蛋白标签：
    Tag type will be determined during the manufacturing process.
    The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.
  • 产品提供形式：
    Lyophilized powder
    Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
  • 复溶：
    We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
  • 储存条件：
    Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
  • 保质期：
    The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
    Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
  • 货期：
    Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
    Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
  • 注意事项：
    Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
  • Datasheet ：
    Please contact us to get it.

产品评价

靶点详情

  • 功能：
    Has in vitro nucleotide phosphodiesterase activity with nucleoside triphosphates, such as ATP. Has in vitro activity with p-nitrophenyl-TMP. Has lower activity with nucleoside diphosphates, and no activity with nucleoside monophosphates. Has in vitro activity with CDP-choline, giving rise to CMP and phosphocholine. Has in vitro activity with CDP-ethanolamine. Does not have sphingomyelin phosphodiesterase activity.
  • 基因功能参考文献：
    1. Activation of pregnane X receptor induces SMPDL3A expression in primary hepatocytes. PMID: 28414139
    2. Site-specific N-glycosylation is essential for the intracellular stability, secretion and activity of human SMPDL3A. PMID: 28104755
    3. Solved the structure of SMPDL3a revealing a calcineurin-like fold. A dimetal site, glycosylation pattern and a disulfide bond network are likely to be conserved also in aSMase. We show that the binuclear site of SMPDL3a is occupied by two Zn(2+) ions. PMID: 26783088
    4. Data indicate that sphingomyelin phosphodiesterase acid-like 3A (SMPDL3A) is a nucleotide phosphodiesterase secreted from cholesterol-loaded macrophages. PMID: 25288789
    5. the induction of SMPDL3A is liver X receptor-dependent and is restricted to human blood cells with no induction observed in mouse cellular systems. PMID: 22810003
    6. ASML3a is involved in the process of bladder tumorigenesis PMID: 12442002

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  • 亚细胞定位：
    Secreted.
  • 蛋白家族：
    Acid sphingomyelinase family
  • 组织特异性：
    Detected in blood serum. Detected in macrophages (at protein level).
  • 数据库链接：

    HGNC: 17389

    OMIM: 610728

    KEGG: hsa:10924

    STRING: 9606.ENSP00000357425

    UniGene: Hs.486357