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  4. Recombinant Human ADP-ribosylation factor-binding protein GGA2 (GGA2)

Recombinant Human ADP-ribosylation factor-binding protein GGA2 (GGA2)

  • 中文名称：
    人GGA2重组蛋白
  • 货号：
    CSB-YP891942HU
  • 规格：
  • 来源：
    Yeast
  • 其他：
  • 中文名称：
    人GGA2重组蛋白
  • 货号：
    CSB-EP891942HU
  • 规格：
  • 来源：
    E.coli
  • 其他：
  • 中文名称：
    人GGA2重组蛋白
  • 货号：
    CSB-EP891942HU-B
  • 规格：
  • 来源：
    E.coli
  • 共轭：
    Avi-tag Biotinylated

    E. coli biotin ligase (BirA) is highly specific in covalently attaching biotin to the 15 amino acid AviTag peptide. This recombinant protein was biotinylated in vivo by AviTag-BirA technology, which method is BriA catalyzes amide linkage between the biotin and the specific lysine of the AviTag.

  • 其他：
  • 中文名称：
    人GGA2重组蛋白
  • 货号：
    CSB-BP891942HU
  • 规格：
  • 来源：
    Baculovirus
  • 其他：
  • 中文名称：
    人GGA2重组蛋白
  • 货号：
    CSB-MP891942HU
  • 规格：
  • 来源：
    Mammalian cell
  • 其他：

产品详情

  • 纯度：
    >85% (SDS-PAGE)
  • 基因名：
    GGA2
  • Uniprot No.：
  • 别名：
    1200007E24Rik; ADP ribosylation factor binding protein 2; ADP ribosylation factor binding protein GGA 2; ADP ribosylation factor binding protein GGA2; ADP-ribosylation factor-binding protein GGA2; ARF-binding protein 2; FLJ20966; Gamma adaptin related protein 2; gamma ear-containing; Gamma-adaptin-related protein 2; GGA 2; Gga2; GGA2_HUMAN; Golgi associated gamma adaptin ear containing ARF binding protein 2; Golgi localized gamma ear containing ARF binding protein 2; Golgi-localized; KIAA1080; Vear; VHS domain and ear domain containing protein ; VHS domain and ear domain of gamma adaptin; VHS domain and ear domain of gamma-adaptin
  • 种属：
    Homo sapiens (Human)
  • 蛋白长度：
    full length protein
  • 表达区域：
    1-613
  • 氨基酸序列
    MAATAVAAAV AGTESAQGPP GPAASLELWL NKATDPSMSE QDWSAIQNFC EQVNTDPNGP THAPWLLAHK IQSPQEKEAL YALTVLEMCM NHCGEKFHSE VAKFRFLNEL IKVLSPKYLG SWATGKVKGR VIEILFSWTV WFPEDIKIRD AYQMLKKQGI IKQDPKLPVD KILPPPSPWP KSSIFDADEE KSKLLTRLLK SNHPEDLQAA NRLIKNLVKE EQEKSEKVSK RVSAVEEVRS HVKVLQEMLS MYRRPGQAPP DQEALQVVYE RCEKLRPTLF RLASDTTDDD DALAEILQAN DLLTQGVLLY KQVMEGRVTF GNRVTSSLGD IPVSRVFQNP AGCMKTCPLI DLEVDNGPAQ MGTVVPSLLH QDLAALGISD APVTGMVSGQ NCCEEKRNPS SSTLPGGGVQ NPSADRNLLD LLSAQPAPCP LNYVSQKSVP KEVPPGTKSS PGWSWEAGPL APSPSSQNTP LAQVFVPLES VKPSSLPPLI VYDRNGFRIL LHFSQTGAPG HPEVQVLLLT MMSTAPQPVW DIMFQVAVPK SMRVKLQPAS SSKLPAFSPL MPPAVISQML LLDNPHKEPI RLRYKLTFNQ GGQPFSEVGE VKDFPDLAVL GAA
  • 蛋白标签：
    Tag type will be determined during the manufacturing process.
    The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.
  • 产品提供形式：
    Lyophilized powder
    Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
  • 复溶：
    We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
  • 储存条件：
    Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
  • 保质期：
    The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
    Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
  • 货期：
    Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
    Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
  • 注意事项：
    Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
  • Datasheet ：
    Please contact us to get it.

产品评价

靶点详情

  • 功能：
    Plays a role in protein sorting and trafficking between the trans-Golgi network (TGN) and endosomes. Mediates the ARF-dependent recruitment of clathrin to the TGN and binds ubiquitinated proteins and membrane cargo molecules with a cytosolic acidic cluster-dileucine (DXXLL) motif. Mediates export of the GPCR receptor ADRA2B to the cell surface. Regulates retrograde transport of phosphorylated form of BACE1 from endosomes to the trans-Golgi network.
  • 基因功能参考文献：
    1. Study shows that reduced expression of GGA3 resultes in accumulation of endocytosed EGFR within enlarged endosomes. GGA2 interacts with EGFR cytoplasmic domain to stabilize the receptor expression and promote cell growth. Finally, GGA2 was upregulated in 30.8% of human hepatocellular carcinomas and 23.3% of colorectal cancers. PMID: 29358589
    2. PI4KIIIbeta interaction with the VHS domain of GGA2 affected PI4KIIIbeta localization. PMID: 28289207
    3. full length alpha2B-AR associated with GGA2 but not GGA1, its third intracellular loop was found to directly interact with both GGA1 and GGA2. More interestingly, further mapping of interaction domains showed that the GGA1 hinge region and the GGA2 GAE domain bound to multiple subdomains of the loop. PMID: 27901063
    4. These data indicate that clathrin is required for the function of AP-1- and GGA-coated carriers at the trans-Golgi network but may be dispensable for outward traffic en route to the plasma membrane. PMID: 24407285
    5. GGA2 mediates a vital function that cannot be compensated for by GGA1and/or GGA3. PMID: 22291915
    6. endocytosis and intracellular transport of memapsin 2, mediated by its cytosolic domain, may involve the binding of GGA1 and GGA2 PMID: 12135764
    7. GGA proteins function with the phosphorylated ACDL in the memasin 2-recycling pathway from endosomes to trans Golgi on the way back to the cell surface. PMID: 15615712
    8. Results demonstrate that cargo proteins contribute to the recruitment of GGA2 onto membranes and to the formation of GGA-positive clathrin-coated vesicles. PMID: 17451558
    9. These results show that the dual roles of PI4P can promote specific GGA targeting and cargo recognition at the trans-Golgi network. PMID: 17494868
    10. p56 tightly cooperates with the GGAs in the sorting of cathepsin D to lysosomes, probably by enabling the movement of GGA-containing transport carriers. PMID: 17596511
    11. GGA2 is recruited to the trans-Golgi network independently of the other Golgi-localized, gamma-ear-containing, ARF-binding proteins and is required for the efficient sorting of lysosomal enzymes PMID: 18431031
    12. GGA overexpression causes various sorting defects as measured by recycling of CD-MPR, internalization of transferrin receptor, and the subcellular localization of proteins like Tsg101, ubiquitin, and Hrs. PMID: 19788741

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  • 亚细胞定位：
    Golgi apparatus, trans-Golgi network membrane; Peripheral membrane protein. Endosome membrane; Peripheral membrane protein. Early endosome membrane; Peripheral membrane protein.
  • 蛋白家族：
    GGA protein family
  • 组织特异性：
    Ubiquitously expressed.
  • 数据库链接：

    HGNC: 16064

    OMIM: 606005

    KEGG: hsa:23062

    STRING: 9606.ENSP00000311962

    UniGene: Hs.460336